Gel Electrophoresis Lab

Teacher Ms. Curley explains how to perform our gel elctrophoresis lab. In this lab (from Carolina Biological), students will separate DNA fragments of three "viral" samples to determine how closely related the samples are (and, perhaps, how virulent the most recent viral outbreak will be).

[@user-lf1fv3uf6s]

This is the only video I was able to understand about gel electrophoresis.😊

[@walterwhite4699]

Really beautiful demonstration. Appreciate it! Thanks!

[@tinam1415]

10/10 would watch again. will probably show it to my class

[@nibrassbio8937]

Thank you for this wonderful video ❤❤❤I like it so much ❤❤❤❤

[@MrSoumyaBanerjee]

Thank you, this was very illuminating.

[@GodiyaMoses]

this is a really informative and detailed video
thank you so much

[@nafisasamad3115]

very easy and informative video, you have explained it very well, thank you ma'am, but no explanation about the addition of ladder and positive control and negative control, plz tell about these too ?

[@ScienceCreAchins]

A DNA ladder is just a standard set of DNA fragments that are various, but known, lengths (bought from a biological supply company). They will be pulled through the gel, with the smallest pulled through faster than the larger fragments. When the gel is disconnected and stained, the ladder can be compared to the unknown fragments in other wells/columns, so their lengths can be estimated. (The ladder is used to make a standard curve with semi-log paper (distance vs. length of fragment), and the unknown fragments can be estimated by using the curve made with the semi-log paper). I don’t think we actually used a ladder or controls for this lab, just the three strains of (fake) virus DNA (it's a 9th grade, intro to biotechnology lab).
Here is a link to the lab directions:
www.cpet.ufl.edu/wp-content/uploads/2013/10/Outbreak_virus_dna_teacher_manual.pdf

[@scolary5147]

thank you for your video and further explanation

[@swapnadeepd7363]

Wonderful video.....I am from the field of Biotechnology and also in the teaching profession....I loved viewing the video😊

[@swapnadeepd7363]

Beautifully made video 😊 I myself am from the field of Biotechnology and also in the teaching profession...loved viewing the video 😊

[@lamie7767]

hey! i am an aspiring biotech major and had some question that i want to ask if you have time!

[@ramkeshsaini9345]

Tell we CDNA library

[@diksha8756]

Awesome

[@AuraNaturae]

Ethidium bromide is orange dye . But here it is voilet which dye it is.

[@082.apoorvanidhiacharya8]

What are used as DNA samples???

[@ScienceCreAchins]

We used DNA from this kit: www.carolina.com/dna-gel-electrophoresis-kits/outbreak-fingerprinting-virus-dna-8-station-kit-dna-only/211204.pr

[@ramkeshsaini9345]

How to attach DNA in vacter

[@vineetpandey5309]

Probably nice video.

[@BarbacoaAppliances818]

Those googles though, lol
I bet her eyes were boiling from steam and pressure lol

[@LarsLarsen77]

Strand? LOL

[@sunitaagnel-3629]

May be Bromophenol blue