very easy and informative video, you have explained it very well, thank you ma'am, but no explanation about the addition of ladder and positive control and negative control, plz tell about these too ?
A DNA ladder is just a standard set of DNA fragments that are various, but known, lengths (bought from a biological supply company). They will be pulled through the gel, with the smallest pulled through faster than the larger fragments. When the gel is disconnected and stained, the ladder can be compared to the unknown fragments in other wells/columns, so their lengths can be estimated. (The ladder is used to make a standard curve with semi-log paper (distance vs. length of fragment), and the unknown fragments can be estimated by using the curve made with the semi-log paper). I don’t think we actually used a ladder or controls for this lab, just the three strains of (fake) virus DNA (it's a 9th grade, intro to biotechnology lab). Here is a link to the lab directions: www.cpet.ufl.edu/wp-content/uploads/2013/10/Outbreak_virus_dna_teacher_manual.pdf
This is the only video I was able to understand about gel electrophoresis.😊
very easy and informative video, you have explained it very well, thank you ma'am, but no explanation about the addition of ladder and positive control and negative control, plz tell about these too ?
A DNA ladder is just a standard set of DNA fragments that are various, but known, lengths (bought from a biological supply company). They will be pulled through the gel, with the smallest pulled through faster than the larger fragments. When the gel is disconnected and stained, the ladder can be compared to the unknown fragments in other wells/columns, so their lengths can be estimated. (The ladder is used to make a standard curve with semi-log paper (distance vs. length of fragment), and the unknown fragments can be estimated by using the curve made with the semi-log paper). I don’t think we actually used a ladder or controls for this lab, just the three strains of (fake) virus DNA (it's a 9th grade, intro to biotechnology lab).
Here is a link to the lab directions:
www.cpet.ufl.edu/wp-content/uploads/2013/10/Outbreak_virus_dna_teacher_manual.pdf
thank you for your video and further explanation
10/10 would watch again. will probably show it to my class
Really beautiful demonstration. Appreciate it! Thanks!
Beautifully made video 😊 I myself am from the field of Biotechnology and also in the teaching profession...loved viewing the video 😊
hey! i am an aspiring biotech major and had some question that i want to ask if you have time!
Wonderful video.....I am from the field of Biotechnology and also in the teaching profession....I loved viewing the video😊
this is a really informative and detailed video
thank you so much
Thank you, this was very illuminating.
Thank you for this wonderful video ❤❤❤I like it so much ❤❤❤❤
Ethidium bromide is orange dye . But here it is voilet which dye it is.
What are used as DNA samples???
We used DNA from this kit: www.carolina.com/dna-gel-electrophoresis-kits/outbreak-fingerprinting-virus-dna-8-station-kit-dna-only/211204.pr
Awesome
Tell we CDNA library
Probably nice video.
May be Bromophenol blue
How to attach DNA in vacter
Those googles though, lol
I bet her eyes were boiling from steam and pressure lol
Strand? LOL