Using a Micropipette - University of Leicester

A short film showing how to use a Gilson Micropipette. The film was made to support the teaching of undergraduate Biological Science students at the University of Leicester.
The film was produced by GENIE, based within the Genetics Department at the University of Leicester. Further information and materials can be found on the GENIE web site www.le.ac.uk/genie and the GENIE VGEC web site www.le.ac.uk/vgec
The film was directed by Jon Shears of ITS-Multimedia Services and the film was produced by Raymond Dalgleish, Nicola Suter-Giorgini and Cas Kramer.

[@DA-js7xz]

I laughed so hard at that sound effect for the "Don't Do's".

[@nicholaspark4165]

The Nos sounded like a broken tuba or a fart

[@burcusatelier]

me too!! the sound...was great! :)

[@seven7wenty]

haha that's how you'll remember the do's and don'ts

[@valeriozippo5720]

poooo poooo X .......... LOL

[@cubehire3653]

This is the best Pipette instructional video I have found thank you.

[@ceciliarosenqvist9797]

Cube Hir. e

[@cmrnqueen]

only pipette video that shows how to read the the P2...Thank YOU!!!...just saved me from getting hell from my lab proff

[@willdrew6825]

This video made me want to micropipette some liquids so bad

[@HZAexNB]

Not as easy as it looks mate

[@denwin4221]

No bro
What my teacher could not explain
Like first stop and second stop
Easily explained by the mam ,for me to understand.
Really helpful ❤️

[@melissarichten6323]

Same

[@Dinlek]

Without a doubt the most helpful and informative lab video I've watched. Thank you very much.

[@gilong1311]

Awesome video, answered all my questions.

[@johannesmthembu8663]

Thanks University of Leicester for this video,well explained thank u.

[@chriswong1516]

How a clear explanation. I'm going to do laboratory practical tomorrow. This may help a lot to me.

[@austindiehl6540]

This video was terrific. Thank you

[@dontownsend9724]

You've covered the possible ways the pipette can be contaminated or damaged,but you've failed to follow up on what to do about it. I started working in an analytical lab over 20 years ago and they were having trouble passing the unknown quantitative metals tests each year required by the EPA. My first year on these analysis's I was testing the output of my eppendorf pipette on an analytical balance with reagent grade water to insure in was delivering the proper volume before making my standards. Another analyst asked me what I was doing , I told them and they then said." well I worked in the medical field for over 10 years and we never tested our pipettes". All I could think was no wonder so many people die in hospitals if this is normal SOP in the medical field. If the pipette is found to be not delivering the set volume it must be taken apart and cleaned or repaired !!!! After this all our measuring equipment was checked closely for accuracy. We never failed our yearly test again .

[@TheSnakeboy11]

Awesome story, that's making the world a better place.

[@jauld360]

The first thing I did on receiving a new pipette was to test it. a) for accuracy, b) for repeatability and c) to check my technique. That analyst wasted 10 years.

[@Hiphop101ize]

Oh yeah, all this talk about the tip got my body tingly!

[@oreadeboye4506]

Thank you so much for the video. You made pipetting so easy to understand.

[@christyxoxo]

lecturer told me to watch..
and i found it very useful!!! Thanks!!

[@marcelomesa2154]

Excellent video, thank you for bringing such a great content, University of Leicester! Subscribed : )

[@jtxc3]

Thank you! This is brilliant! It helped me review for my lab mid-term

[@anjichant543]

That was an excellent revision of my 4 years of study in lab.

[@김규형]

정말 유익해요! 과학 실험을 진행하는데 필요한 기본적인 지식들이 알차게 들어있네요!!

[@cherryy_2468]

this was honestly a really helpful video thank you

[@ifeyinwaobikwelu1314]

A Very Good video, detailed and well explained. this video really helps. thank you

[@KhanaHatake]

I had to watch this for uni, but it's actually so interesting!

[@korenmoscovich4681]

Literally the best pipetting video ever

[@quack703]

This video truly helped me! Thank you!

[@akritino7437]

Thank you for such an informative video ! Loved it :)

[@fabyalauture5929]

Thank you this video explain the process of pipetting very clearly

[@KulbirAhluwalia]

This video is so good 😍😍😍
Covered the smallest details
Awesome work

[@lizziesinclair4254]

Thank you so much for making this video!

[@Nayyarscorner93]

greatest demonstration ive evr seen good job

[@cherylpatel8197]

Such a helpful video! Very thorough.

[@fireemblem2770]

I agree!!

[@zahidwajdan2672]

Best Explanation, and of course Sound Effect.

[@thewirelessbrain9331]

perfect explanation, thank you!

[@nayabghazali5455]

thats the best video i hv found so far!

[@raghadalani9706]

Nice work.. I understand now... Thank you very much 👍👍👌😊😊

[@mohammedahmedjalloh531]

This video is super helpful... so very detailed

[@chauhuynh8158]

Wonderful video, thank you for helping me with my lab

[@yolniejacques8117]

This video is very helpful, thanks!

[@retinitispigmentosawillbec6447]

Thank you so much! A very helpful video!

[@BioLogicalNerd]

Thank you for this, I'm a micropipette pro now!

[@FieldGamers]

This was perfect. Thank you

[@Sp-fe1eo]

thank you so much for the video...it's really cool and easy to understand

[@winterhatharrived2037]

P2 range: 0.2-2ul
P20 range: 2-20ul
P200 range: 20-200ul
P1000 range: 100-1000ul
(in this case u is the symbol for micro, or 10^-6)

[@TheSexyN3RDZ]

"puhpet"

[@essentialsofbiology1643]

Puppette! It's French.
'Whilst' is the worst. Why not 'while,' dear Brits et al.?
But excellent video, thanx Leicester.

[@noshinnamira6608]

this is so detailed, i learned a lot from it

[@Evemaru2]

Best video tutorial I have seen.

[@KingofDundee]

Sounds like Douglas from the IT Crowd, great video!

[@lalayatem3758]

amazing video , thank you and God bless!

[@bhaktisatam726]

Thanks for risking your pipette for us

[@hamismwinyi3723]

Thank you, this video is really helpful

[@lglintott]

Brilliant. Putting this on my teaching lab page.

[@JoChen-gb4xr]

honestly, the part which the video demonstrates dos and don'ts is the best

[@natxnatxnat]

What a brilliantly useful and helpful video. Thank you!

[@selamyemane1640]

You have been very helpful. Thanks

[@hazelezeribe5767]

Soooo Helpful!! Thank you: :)

[@palashmassey]

wonderful...love it and learn it..keep uploading please

[@ifrahjameel4588]

best explanation so far

[@bottledblue]

thanks heaps - helped my prepare for my first molecular biology lab! :)

[@xeryd6037]

same but 6 years later

[@nafis_rk]

@@xeryd6037 same but a month later

[@elenaautumnalmermaid6913]

As a biochemistry student, my heart breaks everytime the liquids touch the actual pipette.
But great video for learning what to do and what not to do.

[@deadbzeus]

I feel this lol

[@kkmatt]

¡Muy excellente! Totally ready for lab!

[@mikejedi182]

Thank you for creating this video

[@sobster123]

Best pippetting vid out there!

[@minasdrakos7544]

excellent, very analytic!

[@yeyhu4767]

excellent video, thank you !

[@Teo_live]

Great video. The [X] don't-do's were entertaining

[@brookebain7250]

Thanks for this video! My professor didn’t go over how to set the volumes and I was so lost

[@JustAethnicDonut]

Thanks, so helpful

[@microfetish]

A lovely job indeed!

[@fatimainigo25]

Best Video! Thank you very much!

[@lucysnowe9974]

Very useful tips, thank you. :)

[@liying1994]

Excellent video.

[@sarahjt1]

Very helpful! Thank you!!!

[@riyadhdahham2075]

Cood... amazing explains
Thank you شكرا

[@razahasanbhatti8454]

Thank you very much very informative video. kindly tell me how to pack culture of nitrifying bacteria in sashay or a bottle actually i am asking about the media some people pack bacteria in powder form which media they use to bring alive bacteria into powder form?

[@thanhmiennguyen895]

Thank you so much. It's useful.

[@lyubomirgizdarski8191]

Great video!

[@ayeshashaikh9729]

Thanks a lot for the best video 😊💗

[@andraskovacs5247]

fantastic video

[@victorenriqueriosortega5917]

Es un video muy interesante sobre el uso de las micropipetas, desconocía qué algunas pipetas tenían en el botón un número lo que nos dice hasta cuanto puede medir, además de los números rojos qué también sirven para lo mismo

[@rabindrakhatiwada3558]

is there genetic engineering lecture series? if yes than please do post it.

[@LoveResidentEvil4]

Thank you :) helps my lab bio a lot :)

[@nxph2108]

So satisfying.

[@Densitometry]

Amazing tutorial.

[@KeptNow]

not bad for an instructional video.
these science instructions are usually 20 years old and out of date. this video was really well done--with camera angles, explanation, and reasonings

[@spkspk1416]

Literally I found gem in the form of this video

[@fisslimen]

Great help!

[@kirad1947]

I have a Biology lab pre-quiz tomorrow, haha thank you

[@CWGminer]

Same

[@joanne7699]

Hi may I ask does the
Pipette need to be vertical at all times even when dispensing liquid and
Does the pipette tip need to be against the wall of the receiving tube during dispensing ?
Thank you

[@huongnguyenvan4020]

thanks! it is very useful!

[@cutler610]

awesome vid thank you

[@charlesvictoria6800]

good video, thank you!

[@roisfatawy1712]

Thank you. You explained properly..

[@Navid115]

awesome video

[@sasa012]

great video ...

[@a.s9509]

If we have to use the second stop to eject the volume into the receiver tube there is going to be bubbles with that which is going to give you inaccurate results when say you are measuring the OD of serum samples. And if we don't use the second stop there would still be liquid in the pipet tips. What do we have to do?

[@soulijm1757]

Thank you so much

[@nadew.02]

Thank you so much

[@chillagunilla]

Great for preparing blotters!

[@ayomideolorunfemi]

such a good video

[@estelamestellaestellaiscry4417]

Excellent PLEASE .give examples of calculation of valume...

[@vanniah1]

Thank you!!!!

[@mdmohibullah5791]

Thank you

[@hewagoda]

Thank you.