Agarose Gel Electrophoresis of DNA fragments amplified using PCR

This video is the third lesson in a series of resources detailing the PCR process and surrounding activities. It shows how to analyse a DNA sample using agarose gel electrophoresis, as well as how to make the agarose gel.
The following links are for similar materials to those used throughout the three stages of this tutorial. These items are not identical to all those used in the video, but should warrant similar results.
0.9% Saline Solution: amzn.to/2xQCoZO
High precision pipettes: amzn.to/2T41An5
Screw cap tubes: amzn.to/2YMVf32
Centrifuge: amzn.to/35GPUeO
Microcentrifuge tubes: amzn.to/3fvLlbV
Thin walled PCR tubes: amzn.to/3fvLlbV
PCR tube rack: amzn.to/2LeVtaV
PCR Cycler: amzn.to/2LdSWhu
Agarose Gel creation:
50x TAE buffer solution: amzn.to/3cn79og (this will need turning into a 1x concentration)
Agar powder: amzn.to/3bbtp2Q
Electrophoresis tray and system: amzn.to/2WkdBqx

[@zmazn]

absolute lifesavers, i had this experiment as my lab report and this video helped me like no other ❤

[@callyx5783]

Because of COVID my science course at college are unable to do certain experiments like PCR and gel electrophoresis, so this account is a life saver for my assignment on them. Thank you!!!

[@godislove8740]

Are your labs being used?

[@maryprojectsph]

@@godislove8740 nnnnnnn

[@maryprojectsph]

@@godislove8740 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnñnnnnnnnnnnñ9n

[@Entertainmenttz360]

Thank you so much, i did it with lab mates and then come here watching it, completely clear understand it, be blessed much!!

[@Anchal187]

Are you in college or school

[@TheWaleedKhalid]

Great video.
It helped me to better understand the concept of Gel Electroforesis.
Thanks ❤️

[@prabhathdeep.m9836]

GEL ELECTROPHORESIS is really easy to understand after seeing this video
Thanks

[@tessemabibo7381]

it is nice.I got enough understand about laboratory session as biotechnology student and thank you!

[@747070]

Never understood how electrophoresis worked by just being told about it, nice to see it for real. Thank you, very thorough video! :)

[@aayushshroff03]

Do u see Newton before studying his law?🙄🤔😄

[@GunCops]

Electrophoresis is the separation of molecules with the use of an electrical field. Every substance, usually a nucleic acid or a protein, has a pI. That's the Isoelectric point and it's a feature that comes from the amount of positive and negative radicals it contains around it's perimeter (if it's a protein) or in it (if its a nucleic acid. Positive radicals move towards the negative pole of the field and negatives - to the postive. If you submerge a gel with the proper pH containing these molecules in a medium that allows an electrical current to pass through, the current will pull the molecules along with it. Heavier molecules stay behind, lighter move faster, thus, a mixture is seperated. Now, the molecular weight (how heavy they are) of a molecule is also its signature. You identify the molecules you need by their molecular weight.

[@nomanshah2491]

Great, I have used same method in my research , it's very sample and very beneficial video, Thanks ♥️

[@user-bi8uh5fq3q]

Thank you for showing the entire procedure , now I understood electrophoresis properly after seeing all the steps... reading it from the book doesn't clear the entire concept of it.

[@yashfaasif938]

Now I understood better reading book is useless after watching this animation it's clear my all concept Thanks for making this Video

[@umknownchannel7244]

which books are you learning for bsc zoology 1st year

[@y.t.7832]

شكراً جزيلاً thank you very much

[@emmanuelgkigkilinis9124]

Very intersting and helpful video! Quick misiterpretation question : In 5:45 you say to add 10 micro-L of control sample (DNA ladder) and then 20 μL of each of the other DNA samples, am I correct?

[@ziaullah4916]

A bundle of thanks

[@mr.musical1853]

Amazing. Thank you 🙏

[@prathibhajay845]

Thank you so much❤... Plz continue to do more videos like this

[@prachetaghosh2308]

Amazing video
Thank you☺I hope that can also do the same in future days✌

[@supershuckel4298]

So u was a neet aspirant

[@NourishHub-zh5lv]

Thank you! it is a helpful video for my experiment.

[@jaisramchaudhari505]

This is helping in reading

[@tejendrarathod8351]

Great video

[@bhushanbhadade9064]

Can we use 1X TBE buffer in to the tank

[@khansaamin8620]

Best way❣️

[@urvashilomas6504]

why etbr or any other visualization dye is not added?

[@lunallena5519]

Muchas gracias i understand very good thank You for help me.

[@jaydenmweshilonga463]

Thanks so much

[@subasubi8145]

I`m.. biotechnology student bsc.. i do'nt know please tell any project topic..what kind of project i.. do?

[@Getgot-en1kv]

May I use QG buffer in agarose gel instead of TBE buffer?

[@renukarenu4954]

Superb👍👍

[@itspooja6181]

Thanks a lot 😊

[@sathisht8596]

The addition of Ethidium Bromide before the solidification of agarose gel was missing.

[@Tufailbhatt]

you forget to add Ethidium Bromide to the conical flask after heating the agarose solution.

[@yermiamokosuli7172]

thank you

[@tony232cool]

why do you need to separate dna by charge after pcr? my boss says pcr can tell us everything that is what they use in forensic files.

[@nisrinejarmoune6415]

Thank you

[@Hurrdurrderpable]

Protips. Use thin wells for sharp bands. Use black paper to ease loading. You need only 1 tip if you wash ur tip in the buffer

[@pratimagahane5112]

best vedeo sir

[@diewo3364]

hello，I want to ask that have you miss the EB or Gel Red?

[@dyanakhan2011]

I also want to know is it ok to skip EB?

[@priyankaflorina8411]

Do they add amplified DNA sample from PCR or only DNA sample?

[@mjchronicles1438]

Great video I think I will pass my lab test

[@nextagro]

Not sure is the comments are still answered, but... Why do we need a PLATINUM wire electrode for the electrophoresis? What exactly will happen if one replaces this to an ordinary Copper wire electrode?

[@IzzaKamalHaridhi]

Thank you so much..

[@thunderthunder9251]

I did the same but after cutting gel to extract the DNA, the concentration was very low, dose anyone knows how to increase it?

[@ujjalpatra1223]

Thanks.

[@eff_rah6475]

Wow great

[@sheikhforid2698]

Thnks..

[@mandavitiwari1073]

Awesome...
😊😊😊😉

[@jpjsjp4870]

Thanks alot.nice explanation with video...

[@priyaprakashu8605]

its very intresting

[@supershuckel4298]

But longer molecules of DNA will have more -ve charge then why it is behind shorter ones

[@iqramalik6031]

In DNA fingerprinting! DNA from gel is transferred to......... For hybridization?

[@goodnessnwanya5372]

Thanks

[@Darko4323]

tips on not piercing the gel when inducing the dna?

[@georgeieboy3875]

this method i trust because you can watch it develop and prove it visually. i don't trust people saying you can hand carry a sequencer and plug it into a laptop. that doesn;t make any sense.

[@zahidbhat5709]

there are master mix reagents where we dont need to add tracking dye , but making gel without etbr i hv nvr seen

[@priyankanagra3637]

Nice👍👍

[@jhonkhalid2608]

Sir upload also video on elisa pcr chromatography centrifugation and dna fingerprinting...

[@preethathilakan5402]

You didnt show elution.

[@ItachiAmiraLucy]

Won't the DNA molecules leave the walls created in the gel to swim in the TAE buffer ?

[@liniencemaposa8677]

No, because they are being pulled in one direction by the current.

[@Nityashah492]

Why don't you mix agarose with etbr

[@satheesanek932]

Good

[@dyanakhan2011]

Can we use 2 micro- L dna ladder with 5 Micro- L of pcr product/sample?

[@ambikaheerekar2158]

How about addition of ethidium bromide???

[@divyar8063]

Yeah, I think they forgot to mention about it in the video..

[@MatTroiLuoi]

Can I ask how many liters the microwave is ?

[@preethathilakan5402]

How can we see the DNA without exposing it to UV radiation???

[@adi6899]

for that your going to need a microscope

[@cynthiarao8324]

U can't see DNA in visible light

[@Woanne28]

Where is the gelred or gelgreen?

[@marsimohi6920]

Best to gain better knowledge about........

[@ritikawaghmare8822]

uh did not added etbr

[@soummi1]

How can we see the DNA without the EtBr i.e intercalating agent which was not being added in the Agarose gel ?

[@TheScharda]

Indeed . Thatswhat i was thinking too . We used Gelred instead of EtBr.

[@kartikeyvishnoi2978]

Nivedita Mitra he did say that the gel is ready to be stained. Though he did not showed the process.

[@DivyaSharma-vj4vz]

Loading dye is also called ETBR

[@dipanwitasarkar6285]

yess loading dye is also called Etbr

[@jhonkhalid2608]

Loading dye ****?????

[@Biswajit_Goswami]

Thanks.. helpful

[@awatifalahmad6024]

اريد باللغه العربيه نفس التجربه

[@prettyladyp2318]

Which loading dye did you use?

[@preethathilakan5402]

Ethidium Bromide

[@trump408]

@@preethathilakan5402 😲 it's too toxic... It's not suggested for use, better SYBR Green

[@vaibhavmhaske3480]

Thanks a lot

[@ristusjeesus]

you are so cute and pcr goes firmly

[@dhruvitvadher6955]

thank you kevane laayak nathi tu

[@hassanyousaf7824]

its gud video but you did n't tell about the loading dye and tracking dye.Try to give complete concept about experiment...

[@animeenjoyer9382]

THANK YOU

[@karimabdelbary1898]

شكرا

[@user-dq9ic5bx2c]

كنت اظن نفسي الوحيد هنا الذي يتكلم اللغة العربية

[@lavadibo3987]

@@user-dq9ic5bx2c نو لست لوحدك

[@sayantansaha53]

Can we use TBE instead of TAE?

[@ahamedtahur8357]

Yup

[@maryamghuman8852]

yes u miss etbr

[@abed0019]

You forget put ethidiom bromaid in liqued gel befor rins it

[@awanimillavithanachchi931]

if can disply the procedure by words while play the video good

[@noname-qn1lf]

What happens when we eat a DNA

[@princetonseparations7241]

Hey all! If anyone's interested, check out our channel to see Electro-Sep - a product that can capture DNA bands in an agarose gel!

[@omsingharjit]

7:23 can we see it just by naked eyes ?

[@arnavgandhi2942]

No, u need UV light

[@omsingharjit]

@@arnavgandhi2942 is dna Fluorescence ?

[@soumyanr2442]

Etbr?😶

[@Mircovoice]

I think she broke the walls in the gell

[@jameelgee1859]

Plz explain it in Urdu

[@puneethnagaraj796]

I'm from mit

[@williambdagoseh541]

Barkai wayy

[@arresteddevelopment2158]

Why do I always say Methyl A instead of Poly A tail? Sheesh

[@jsvclubdeciencia6283]

In silico: ruclips.net/video/BkTRYMjyatA/видео.html

[@MannansElearning2021]

ruclips.net/video/Fv-vayq5MCA/видео.html (Watch this video for precautions)

[@baapsabka7615]

Everywhere I go I found loads of Indians in the comment section

[@areebakhan141]

That is according to ur location set in the yt settings

[@meenaeghazal3155]

These basterds are almost Avery where

[@vaishnavipawar1603]

Try muting the video ☕

[@kichu2555]

Any class 12 Bois

[@lincolnoliveira3041]

Rmddu

[@cansunuryaylacoglu6272]

Pls add Turkish language

[@skilvarajput3151]

average

[@tessemabibo7381]

it is nice.I got enough understand about laboratory session as biotechnology student and thank you!

[@vinayvinni6827]

R u BSC student

[@sagarsagar3397]

Thanks

[@asclepius-thegodofmedicine3810]

Thanks