Electrophoresis
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- Опубликовано: 20 июн 2015
- Electrophoresis lecture - explains about the gel electrophoresis principle and the role of electrophoresis in separating DNA and proteins using agarose gel and sds page. Gel electrophoresis is a process for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, founded on their size and charge.
This video lecture also explains the types of electrophoresis like agarose gel electrophoresis and SDS PAGE.
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Thank you for watching the biology techniques lecture on electrophoresis.
Thank you , u have the blessings of making ppl understand easily
Thank you! This video really cleared things up.
thanks again sir, your videos are the best
Thank you so much. You are a life saver.
thank you very much for your efforts
nice video. thank you from hong kong. :)
شكرا لك كثير...لقد امتعتنا في الحديث عن كل انواع الكتروفوراس
u speak very well gentlman...i like the way u explain
Thank you Mr. for nice presentation but how we can know weather our electrolysis is contaminated or not and should the positive controls must have lager size than to the samples how we intemperate
Thank you sir your videos are always awesome !
great sir ...mindblowing
Amazing you are !!
thankyou for the video.
Super !! .... As always !!
Thank you so much !!!
+Amelou Melou thank you. Glad you liked my lectures
thankyu sir bahuuuuuuuuuut achcha smjhate ho ap .great ho ap
+shama shahid glad you liked it
Hello Sir, I want to know which protein or DNA size moves faster or slower in electrophoresis? because in some lectures smaller are moving fast and in other lecture larger is moving fast...... Please explain and clarify
Thank you
very nice and simple explanation of electrophoresis...
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you.. molecular biology exam chelo , valo vabe bujte parlam..
You're welcome. Glad to hear that you're getting benefit from my lectures
sir add some video of data analysis using image lab software (gel doc imager) how can we obtain sequence of dna using this software
thank u sir, it is good as always🙂
Topic send me
youre doing a great job. really appreciate it 💯respect
+Sushma Agravat thank you very much. Glad you liked my lectures
Would you tell me plz from which book you have taken this topic
You are just 🔥❤ @shomu sir
Thank you
Plz tell me about the book from which i prepared notes of electrophoresis
This has been helpful
You're welcome
thank you sir good lecture and essay language
+Kalamuddin Ansari glad it helped
Nice video thank you sir ☺️
You're welcome. Glad to hear that you're getting benefit from my lectures
Sir, if the particles are separated by size from the bigger to smaller, does that mean the holes in the gel are gradually smaller? Because if all the holes have the same size, i think the particles will divide into to side: the large ones can not pass the hole and the small one can.
If you wish to maintain this model, imagine that polymers that are too large for the holes can fold on themselves to fit through a hole. The time it takes for a longer polymer to properly fold to fit through a hole is longer than the time a smaller polymer would have taken to pass through since no reorientation was necessary.
To be more accurate:
Every polymer must fold in some way to fit through the hole. The shorter the polymer, the less likely it is to require folding to pass through the mesh.
Thanks sir
You're welcome
ur the best
Thank you. Glad you liked my lectures
hello sir plz explain me these...
While migrating from negative to positive end , how can we make sure that our dna dont come out of the gel ? and why we cannt load dna samples in the wells before loading the buffer?
We tag the DNA with stain so that we can see its movement in gel and we stop its flow after it reaches a certain distance.
If we load buffer after loading DNA, it will cause DNA to come out of the wells
Because if you add before the buffer then DNA will denature
how we tag protein into a negative and positive charge?
why we use etbr? is there a special reason
Thank you very much.. I finally understand
keep it up
+syed sahil thank you
Thank u sir
You're welcome
hindi medium m ho sakta G
h...
Tq sir
You're welcome
How can i thank u!
You're welcome
what do u do in ur personal life? Are u a teacher? Or PhD student? Or doing job?
Sir please is vedio ko hindi me bhi banaiye na
Okay
Sir hindi vedio daliye
Hindi videos are coming
Cathode is positive charge molecule !
Firstly go and correct it 🤗🤗
Cation is positively charged. Cathode is negative. Clear your concept from the basics.
Insufficient knowledge
Thank you sir
You're welcome