Ma'am you are an incredible proff how gifted u are in teaching so I wanted you more to upload on some videos.thank you ma'am I m really getting so many things from your teachings
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
@@udkmyname5251 proteins are different from dna as the dna goes through transcription and translation to produce protein. proteins are made of amino acids and dna is from nucleotides
Southern blotting is named after British biologist Edwin Southern. So the the first letter of Southern blotting is capitalized....northern blotting and western blotting are not associated with names of any scientist but named so to keep similarity with 'Southern'.
Thank you so much ma'am.... students who can't afford for coachings can visit this .... I'll recommend about you whoever asks me how to understand biology concepts......i respect you and appreciate your efforts.. thank you Madam ❤️❤️
thnks.. i m getting a lot of help from it.. as my bio teacher got transferd to another school.. so i m using your videos to make my concepts.... thnks again :-)
Hi mam.. I think u went wrong about this topic. Isoelectric focusing is used only in 2DGE but here we have only 1DGE process which is only based on electrophoretic mobility of nucleic acids which don't require pI .. as it has common surface negative charge.please go through it once again
Maam I have a question how we decide which sequence should be der in a dna probe ??? Or if we take any random sequence how can v be sure that this random sequence is also present in our dna fragmets....
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Mam...i have a question..from transfer assembly the Effinity of both gel and nitrocellulose is less the DNA but From nitrocellulose DNA was not moved and from gel DNA was moved why??
When separating DNA fragments vertically on the basis of Density Centrifugation, are large DNA molecules stay up in the agarose gel or they are gonna come down as happens in heavy density..?
Good evening ma'am. Your teaching is very excellent but I'm getting confused about sequential steps. Which step is first 2nd,3rd and so on especially in biotechnology. Please Clarify it madam.
How do I make the appropriate DNA probe? I mean, if i already know what is its complementary strand then why do I need to check it by X-ray radiography!
I am sorry but Southern, Western and Northern blots are not just electrophoresis separations. They do include separation in their protocol, but that is just the first step.
prof. I would like to request you if you can upload some videos on how to read the phylogenetic tree and different types of selection in detail! I will appreciate it.
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Wanted to ask a question. In the separation of fragments you didn't mention any type of electric field applied . Then how the fragments will travel into the gel on the basis of their sizes or densities??
thanku so much mam my humble request for you to upload vedios about molecular markers particularly RFLP ,RAPD ... PLEASE MAM.. thanking you in anticipation
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Ma'am you are an incredible proff how gifted u are in teaching so I wanted you more to upload on some videos.thank you ma'am I m really getting so many things from your teachings
I m believe in self study and u help me a lot to gain a perfect knowledge
The explanation is very simple manner, and I can easily caught the points. Thank you so much mam👍
Goddess Saraswati, as if descended on Earth!!
😊👍😌
really it is true bro/sis/trans
@@puneethnagaraj796 I'm glad, that u agreed with my notion, trans/sis/bro😊
Sorry sis i really disappointed seeing your replay
I shouldn't write that comment
@@puneethnagaraj796 🌝😌
Ma'am if we will have teacher like you in our schools we students will never miss any class and will love every subject ❤️
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Hi as far as I know, isoelectric focusing technique used for protein separation not for DNA
Ahmed Ali dts true
Well as far as I have understood proteins are segment of DNA and this method deals with the fragments of dna so it's proteins are basically 🧬 🙂
@@udkmyname5251 proteins are different from dna as the dna goes through transcription and translation to produce protein. proteins are made of amino acids and dna is from nucleotides
@@shreedhamji6554 true....yeah you're right
Ty for correcting 🥂
Hello guzs i am from food technology so can i right it same as she describe in examination?
Tnak u mam.... Your teaching style really nice....... It helps me a lot
This woman is so brilliant.. thank you ma'am
Ma'am I would like to bring to your notice that we do not dissolve agarose in distilled water instead we use TAE or TBE buffer.
.
What is buffer..
In my lab we always used distilled water to dissolve agarose. The buffer was only used in the electrophoresis.
@@MultiLeandrini S...right
Southern blotting is named after British biologist Edwin Southern. So the the first letter of Southern blotting is capitalized....northern blotting and western blotting are not associated with names of any scientist but named so to keep similarity with 'Southern'.
Thank you so much ma'am.... students who can't afford for coachings can visit this .... I'll recommend about you whoever asks me how to understand biology concepts......i respect you and appreciate your efforts.. thank you Madam ❤️❤️
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET
Her approach is so captivating. She is a treasure.
Mam can you please add more video explanation for NET exam purpose because ur explanation is really easily understandable .
Thanku mam😊
way of teaching is really awesome
Theivame !! One like is not enough.👍
why DNA has different negative charge?
please do human health and disease
thnks.. i m getting a lot of help from it.. as my bio teacher got transferd to another school.. so i m using your videos to make my concepts.... thnks again :-)
Mam u used term buffer here. which buffer we use here?
Hi mam.. I think u went wrong about this topic. Isoelectric focusing is used only in 2DGE but here we have only 1DGE process which is only based on electrophoretic mobility of nucleic acids which don't require pI .. as it has common surface negative charge.please go through it once again
yes exactly she need to correct it. IP does not imply for DNA molecule its for proteins
I would request you to read ncert pls
Mam. Wat does ncert diagram 11.3 mean by digested and undigested dna?
Maam I have a question how we decide which sequence should be der in a dna probe ??? Or if we take any random sequence how can v be sure that this random sequence is also present in our dna fragmets....
Very good lectures madam
God bless you !
From srilanka 🇱🇰 🙏
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
you are incredible ma'am
Why we need to boil agarose? Rather than normal dissolving with buffer.
which buffer is talked about here mam???
According to me two buffer were uesd....Tris borate EDTA and Tris acetate EDTA
Thanku so much for the videos mam, but the ecology portion videos are not available on you tube plz upload videos of ecology also
Buffer in transfer assembly is made of? How it goes up
Pls ans
mam your study notes are not available on amazon as they are out of stock so mam can you please tell when would the notes be available again.
Mam...i have a question..from transfer assembly the Effinity of both gel and nitrocellulose is less the DNA but From nitrocellulose DNA was not moved and from gel DNA was moved why??
please explain the interpretation of how to read results
Mam can I ask a question that how is probe going to attach with DNA if DNA is double stranded???
it will not attach to dna instead it will go toward the dna and form hydrogen bond with it
due to which we can judge the sequence of other strand
What is eastern blotting?
Mam please give a chance to take screenshot..
Many students make notes from your videos
When separating DNA fragments vertically on the basis of Density Centrifugation, are large DNA molecules stay up in the agarose gel or they are gonna come down as happens in heavy density..?
Thank you mam for spending hours in preparing this useful video
Good evening ma'am. Your teaching is very excellent but I'm getting confused about sequential steps. Which step is first 2nd,3rd and so on especially in biotechnology. Please Clarify it madam.
what is a buffer?
How do you make probe for that DNA to which probe binds although u don't know the sequence !
How do I make the appropriate DNA probe? I mean, if i already know what is its complementary strand then why do I need to check it by X-ray radiography!
Mam plz upload the blotting techniques
I am sorry but Southern, Western and Northern blots are not just electrophoresis separations. They do include separation in their protocol, but that is just the first step.
I am new to this channel. Are these videos based on ncert reader, cbse board ?
mam please tells about agrobacterium tumefaciens genetic information and functions
I love way you r teaching mam so accurate ....
Mam Isoelectric focusing we use for protein not for DNA.... We use electric gradient for protein 1D separation not for DNA....
Madam how can I get hard copy for Gel Electrophoresis Topic.
Maam aap itne easily samjhate ho😊
What we are separating by gel electophorasis
Mam video 2 ,3 ,4 is not available
ALLAH bless u .I always follow u ...now I'm lecturer botany
prof. I would like to request you if you can upload some videos on how to read the phylogenetic tree and different types of selection in detail! I will appreciate it.
Asli teacher to ye hai 👌👌👌👌
Aaj kal koi kaam nahi mila to bas teacher ban jao
Bhale aata ho ya na ho
Paisa aana chaihiye 😠😠😠
your classes are really helpful
thank you teacher...
Thank you madam 😇🙏🏻
mam can u plz explain bloting techniques.?.....plz mam...
Can any one tell what is buffer about which maam is talking at 20:37
that can resist Ph change upon the addition of an acidic ao basic components
MAM what is flush ends,sticky& blunt ends can you give examples for these plz....MAM
Thank u soo much this helped me a lot in my exam hope I score well
Ur examination is very nice & marvelous
Super awesome video. Many thanks! and one question: how do we know what DNA probe to put in?
mam
in gel electrophoresis does Dna is separated on basis of size as wall as charges??? so cofused
brother dna is seperated on the basis of charge by ion exchange chromatography and on size by size exclusion chromatography
With gel electrophoresis only on basis of size separation
yes we separate according to charge aur size with this technique
@@deepagandhi5076 No according to charge we can also separate the DNA....isoelectric focusing
Very nice and informative.
really nice.i naver give any coment any vedio i open my gmail and suscribe and like u know why . coz i understand all first time
thanku mam
Well understood mam.
Now clear 👍👍👍
Thank you so much ma'am.. You are simply fabulous!!
Thank you mam ❤
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Excellent teaching style. Thank you mam
mam in gel electrophoresis , are we separating rDNA ??
rDNA is a technique recombinant DNA technology
Thanks mem meri mem ne bhut difficult pdaya but Apne very easy Kiya
thank you mam...we got ideas very clearly
I dont explain your importance but i told your talent somethin different.i am very very thankful
Mam plz ans my que
How is DNA isolated in purified form
Mam plzz add a lecture on chromatography..it would be a great help.
ma'am you are teaching are so great
Would be evn less if i thank u mam,seriously uve helped so much . Just cmplimented my self studies.still can juss thank .thnku so much
Dushyant Katara
what is buffer
Please make any other biotechnology lesson in mgkvp bsc 1st year syllabus
U make it all simple to understand thank-you mam
very useful video mam thank you so much😊
Aabha u look soo pretty💖💖
Mam u r like a god to me🙏🙏🙏🙏
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Thank u soo much maam ....u r grrt ....love frm kashmir💚
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
So simply discussed, I am unable to say anything as I am already a bio teacher 🙏
AWESOME
.... FULLY HELPFUL.
THANK YOU
Please teach remaining lessons as much as possible 😍 😊 😋 😎 😊 mam please
Awsm nd thnku for dis tutorial 😊
Mam what is buffer
Buffer....that can resist pH change upon the addition of an acidic or basic components
Mam it's an excellent and wonderful class.Thankyou mam
your work is amazing and simplified.
This video is very helpful for me... Thanks.....
Wanted to ask a question.
In the separation of fragments you didn't mention any type of electric field applied . Then how the fragments will travel into the gel on the basis of their sizes or densities??
Of course we can apply the voltage that's why the DNA was run on the gel if we not apply then there is no movement
very nice explanation of all the topics
Tq so much Mam ...we easily catch u r points..from bottom of my heart tq soooo much Mam..
9:43
thanks madam for ur teaching
Thank you so much mam this video is very useful.
thanku so much mam
my humble request for you to upload vedios about molecular markers particularly RFLP ,RAPD ...
PLEASE MAM..
thanking you in anticipation
Reealy informative..
superb lec💖
How the DNA probe gets attached to double stranded DNA fragments......
I want to know that too..
Do you know what buffer means here
sure omg har jagah buffer puch rkha hai tumne ....equilibrium of class 11 chemistry padho
Phle usko single strand krge Alkaline medium add krke
there is no double standard DNA present only single standard was present.....those are complementary DNA with that probe they were attached.....