So overall, if I have a mixture of bacteria and I n eed to isolate only the Klebsiella pneumonia, how do I do that? Would MacConkey agar plates with lactose be enough to select and differentiate K. pneumonia from rest of the microbes?
Not necessarily. K. pneumoniae appears as a mucoid lactose fermenter on MacConkey agar, but other types of bacteria could be similar. There may be other media, like the one here, better for definitive identification. www.ncbi.nlm.nih.gov/pmc/articles/PMC273960/
The CV forms a bulky complex with the peptidoglycan layer, cross-linking a lot of it tightly together and interfering with function. It is explained a bit more here: onlinelibrary.wiley.com/doi/abs/10.1111/j.2042-7158.1967.tb09550.x
Hi ! great videos. Thanks. Would you tell me please why we are talking about Fermentation "and not respiration" when we describe the Macconkey utilisation of lactose ? especially because of its occuring in an aerobic enviroment which is the surface of the agar. Thanks so much in advance for your reply
Many bacteria still use fermentation even in the presence of oxygen, these are called aerotolerant anaerobes and include lactobacilli and streptococci. This type of agar changes color with the production of those fermentation products. en.m.wikipedia.org/wiki/Aerotolerant_anaerobe
hi! can anyone please tell me if MacConkey Agar is chemically defined or chemically complexed media? from what I have seen, it has defined chemical components and based of that I think its chemically defined. but there are many internet sources which say it is chemically complexed. I would highly appreciate if someone could guide me in this regard,.
Hi, i just saw your video for pseudogenes then the central dogma and now this video and PROFESSOR YOU GONE SLIM, smart, cute, slim, girl thats something you should be proud of!!!. im so proud
Thanks for the suggestion! I try to do videos that haven't already been done by another channel and it looks like there are several videos on the Lowry method: ruclips.net/user/results?search_query=Lowry+protein Let me know if you have other suggestions!
ur kind of saving my life in micro rn and i am really grateful! thank u
Omg, I love you so much. My teacher made them DRY and you made them interested!
Cool, happy to help! :)
This was very well explained and informative thank you so much Dr
Thanks :)
Thanks so much Professor!
Very simple accurate information.
Thank you so much. You made it so easy to understand.
I love to hear that. Thank you for telling me! :)
@@BiologyProfessor :)
Can you make a video like this on CLED AGAR as well please
Nice explanation 👍
So overall, if I have a mixture of bacteria and I n
eed to isolate only the Klebsiella pneumonia, how do I do that? Would MacConkey agar plates with lactose be enough to select and differentiate K. pneumonia from rest of the microbes?
Not necessarily. K. pneumoniae appears as a mucoid lactose fermenter on MacConkey agar, but other types of bacteria could be similar. There may be other media, like the one here, better for definitive identification.
www.ncbi.nlm.nih.gov/pmc/articles/PMC273960/
Ya this was absolutely fantastic.
Thank you!
Thank you!
Thank you for sharing 👍 to helpful for me.
whats the mechanism for how crystal violet inhibits gram-positive growth? I tried searching for answers but couldn't find much
The CV forms a bulky complex with the peptidoglycan layer, cross-linking a lot of it tightly together and interfering with function. It is explained a bit more here: onlinelibrary.wiley.com/doi/abs/10.1111/j.2042-7158.1967.tb09550.x
Thank you for this great lesson ❤️
You are welcome. Thanks for watching!
that is very fantastic
Hi ! great videos. Thanks. Would you tell me please why we are talking about Fermentation "and not respiration" when we describe the Macconkey utilisation of lactose ? especially because of its occuring in an aerobic enviroment which is the surface of the agar.
Thanks so much in advance for your reply
Many bacteria still use fermentation even in the presence of oxygen, these are called aerotolerant anaerobes and include lactobacilli and streptococci. This type of agar changes color with the production of those fermentation products. en.m.wikipedia.org/wiki/Aerotolerant_anaerobe
Wow very amazing , thank alot dr💝
All the MacConkey plates I have ever used are light pink not pale yellow..
This helped a lot! You’re great! Thanks! :-)
Awesome video, help my a lot
hi! can anyone please tell me if MacConkey Agar is chemically defined or chemically complexed media? from what I have seen, it has defined chemical components and based of that I think its chemically defined. but there are many internet sources which say it is chemically complexed. I would highly appreciate if someone could guide me in this regard,.
I believe it is chemically complex. It is complex or undefined due to the digests of animal tissue, and bile salts in the medium.
Thank you ma'am....
I have many doubts in my mind, but you have solved them all...
I’m glad! 😃
Hi, i just saw your video for pseudogenes then the central dogma and now this video and PROFESSOR YOU GONE SLIM, smart, cute, slim, girl thats something you should be proud of!!!. im so proud
can you explain chocolate agar , please ?
I’ve added it to my list. :)
Biology Professor Thank you 🙏
no offense , you should probably make a video how did you manage to loose weight
Keto diet ;)
Hi my name is mehmet i am a phd student in Turkey i have a youtube channel like yours
I watch your videos that give me information that i need
Please do a video illustrating the complete chemical mechanism of the Lowry protein assay (room temperature method)
Thanks for the suggestion! I try to do videos that haven't already been done by another channel and it looks like there are several videos on the Lowry method: ruclips.net/user/results?search_query=Lowry+protein
Let me know if you have other suggestions!
@@BiologyProfessor the top result was not exclusive to only Lowry method so it got kinda confusing and I am not that smart already
Good evening ma'am
Hi :)
Where I can follow you...???
vishnu singh May be in knowledge
What's the use of knowledge in front of godess
@@vishnusingh-zo7cu saale tharki
You have gone slim...😅
i was here to update my knowledge , but thing which is updated is my Crush