CRISPR and
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- Опубликовано: 15 дек 2016
- Today we explore CRISPR! What is it, how do scientists use it, and what does a CRISPR experiment look like in the lab?
Huge thank you to the Google Making & Science team and their #ScienceGoals campaign for making this video possible! None of this would have happened without their support! Also huge thank yous to the Stanford at the Tech program for sharing their yeast with me!
Animation Studio: Zedem Media - www.zedemanimations.com
Directed by: Michael Kalopedis
Interview Videography:
Elliott Morin
3MotionCreative.com
Narration Setup for CRISPR Animation:
Neil Hodge
Script Edits:
Rob Pilkington
Trying to document grad school one RUclips video at a time, from lab equipment to genetics lessons to interviews with other students! Each week is a new view into life as a grad student, and the rollercoaster that is getting a PhD.
Twitter: @AlexDainis
Instagram: Alex.Dainis
Facebook: BiteSciZed
Music:
Pamgaea and Kawai Kitsune by Kevin MacLeod (incompetech.com)
Licensed under Creative Commons: By Attribution 3.0 License
creativecommons.org/licenses/b...
Thank you to DCMP for captioning! dcmp.org/
(All thoughts and opinions are my own and do not reflect the thoughts or opinions of my institution.) 
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That is the best video about CRISPR I've seen so far!!!
I'm so glad you liked it :)
you should do more interviews! Most of us have no real access to what is actually being researched, it is cool to hear what little piece of the puzzle they are trying to solve.
I've got more lined up and coming soon :)
Absolutely agreed. This is also a good resource that can be pointed-to when doomsday-nay-sayers go off on a tirade saying "OMFG BIOLOGISTS ARE TOYING WITH NATURE AND GMOS AND THE WORLD IS GONNA END!!!"
Because the reality is a lot more tame. No one is trying to make babies with four eyes here.
I love that you said SHOT TO THE POINT . Thats gives me more knolages . Thank you alex 🧪🧬
Great video! I really like how you included the demonstration with red yeast. Unlike many videos about CRISPR where the theory is only explained in the abstract, it made the theory very concrete for me. Thanks for posting!
Wow, Alex! This is a very well made video!
At first I was thinking not another CRISPR video... But honestly best CRISPR video yet! The only thing I would possibly add is the fact that anyone can go online and buy a CRISPR kit! Love your videos! You are so inspiring.
Alex this video is amazing. I know some people working with CRISPR in archaea. Will definitely show them this video, it´s incredibly well made.
The ending was gold!
OMG, doing science? Interviews? This is great!
This is a great video. Thanks for contributing to maiking RUclips an awesome place to learn.
Alexis I know almost nothing about genetics but since coming to ur channel you have brought me interesting and entertaining knowledge about basic genetics. Thanks for making my life a bit more interesting.
Great production value!
That's Google, I suppose...
Clearly Really Important Stuff People Research exactly! I can't imagine what Microbiology will look like in 10 years.
Thank you so much for making this video! I've been wanting to find out about CRISPR and Wikipedia was too complicated so this made it at just right level. Also to find out about all the different applications for it. It's much wider than I could have ever imagined!
I LOVED this video! Thanks for including interviews on what researchers are doing! I love seeing people who are passionate and excited about things share those passions.
Thank you Alex for making this. absolutely amazing video, you can clearly see the effort you put Into this, the animation, the demonstration and all those lovely researcher with their amazing work. I'm always glad when I see your videos pop up in my subscription box. also this really helps with my genetics final on Tuesday 👍
Fantastic video Alex!
Brilliant video! Keep up the work.
Love love LOVED this video!! I'm still in school, but you inspire me so much
Great video, loved the animations
Fantastic CRISPR video! :)
A nice "from scratch" list of reading so we can understand every single concept will be appreciated, I'm refreshing all my chemistry classes from college, I will change a little things to include biochemistry and genetics.
The topic of my essay final for my bioethics class was on genetic enhancement through the use of CRISPR-Cas9. Out of all the videos I watched, this was the only one that explained the process so clearly I could actually grasp the concept. Thanks for the video!!
I'm so happy that it helped!! And certainly a fascinating essay topic!
Perfect👌🏾.. The script,animation, montage, lab work, I truly appreciate the effort you put into this and how great the video resulted 💜🙏🏽
Aww, thank you! I'm so glad you liked it!
Very well done video. I learned a lot. Thanks!
Your videos have a very high production value, they should have way more views! Keep up the good work, your channel is awesome :)
Awesome video Alex!
Perfect !
Cool video! Its well edited and easy to understand :)
Oh, this is exciting! I'm watching as I type (well, paused it) but I've caught BBC programmes that introduce these concepts... looking forward to seeing where you take the subject in this video and how you're maybe getting involved! Hope everything's awesome over there, Alex :D
This is such a good video and I feel like I've learnt a lot! New goal is to make my own scicomm this engaging :)
Very cool! More of this!
Working on it now :)
More of this video 👌❤💕
wow your best video yet.
Great vid! :D
Jessica Chang is hilarious!
3:47 that's some fancy equipment there.
Hah that incubator is so ancient. Every time I open that lid and lean in I'm terrified it's going to fall closed and eat me...
More of these videos! If you could do some stuff on recombinant DNA technology and/or gene therapy, in general, that would be great.
love your videos! what editing software do you use? finalcutpro?
I love the Adobe Suite (these videos are usually a combination of Premiere, After Effects, and Illustrator) though I've used Final Cut in the past and enjoy working with it as well!
It's interesting that these things are palindromic. It's somewhat redundant but necessarily so.
And here I am, a simpleton, who thought a crispr was a drawer in my fridge.
Pretty nice video on CRISPR. I don't know if it was the techniques your fellow grad students, but isn't the CRISPR method still fairly inaccurate? I remember toward the end of my last program that I was preparing to possibly use CRISPR to silence a gene and I had 8 off-target sites. But then again it could have been the technique (I think it was developed by MIT). I don't think it was due to the organism complexity because I was working with C. elegans.
The #ScienceGoals playlist hasn't yet been updated to include your video.
Thanks for explaining what CRISPR is actually doing where it was found. I only ever saw videos and popular science articles about how it was now used, but nobody really bothered to explain where it did come from. I always thought CAS9 was actually like synonymous with CRISPR because it is used that way a lot, but now I think it only describes one particular type of CAS protein. Is that correct? Do have an estimate how many different CRISPR systems are known/researched right now? And one last question: do you know anything about the evolutionary history of these CRISPR systems? About where they come from?
Cas9 *is* often used synonymously, you're right, though it's not the same thing! For simplicity, even scientists often use them loosely in conversation: we'll talk about putting crispr into something, or crispring something, when we really mean cas9 or another cas protein. And you're right, cas9 is a specific cas protein from the most well studied/well used system. Other cas proteins from the same system help to actually do the "stealing and storing" of information. As to how many different systems are known and researched right now, my best guess is: lots! There was first one big one (S. pyogenes, where the well-used cas9 is from) but now, as Sukrit mentioned, we're discovering more at an astounding pace! Some with smaller cas proteins, some that target RNA, some whose functions we don't even know! Once we knew what to look for, we discovered multitudes! As to the evolutionary history, I've never actually looked into that, though I'm positive many labs have. A future video I think...
Thank you so much for your answer! A video about that would really be cool!
how do you make the graphics of the crispr simulation at the beginning? what software Is that
That animation was done by the amazing Zedem Media! They're linked in the description :)
that's especially relevant now that it has been (allegedly) used for editing human genes.
According to Jennifer Doudna, the CRISPR/CAS9 protein requires a 20 nucleotide RNA sequence to uniquely define a section of DNA.. but how is it determined where one gene ends and another begins? i.e. each gene length?
Also, assuming you can target a specific organ/tissue, is the idea that the genetic cut/replace needs to take place in 100% of those cells?
Do you have any thoughts on if/how epigenetics fits into all this? Making a gene edit still requires another mechanism on top to express it, no? How does it differ from RNAi/RISC complex?
BTW, on the Intelligence Squared episode ("End of Antibiotics?"), they mention how viruses have developed a strategy against the bacteria's Crispr/Cas9 mechanism!
There was also an interesting article in theatlantic entitled "You May Already be Immune to CRISPR" that talks about how the bacterial protein used to deliver the edits, is killed off by the immune system before it even starts!
Regarding how it's determined where one (of the 25k) human genes starts/stops, there's a video called 'The Noncoding Genome: Finding Treasures in our Junk DNA' that talks about (9m mark) the markers that identify dna segments, as well what could be the use for the 95% "junk/satellite" dna.. i.e. the majority of the 500 million base pairs for which don't seem to result in any rna/protein transcription.
😍Plzzzz someone tell me the name of that music from 4:30 to 11:00
hello there i am looking for
- two of every animal on earth
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any suggestions?
Do you think crispr will be the key to a hiv cure?
Make new videos. For 2018 and let us know if we can ask your doctor about this and how we get it. And when would it be available and how far you come. And include the date. That this film was made.
Hey Alex, could you please turn on crowd-sourced subtitles for this video? I would love to translate it into Portuguese
They are now on! I'm embarrassed to say that I thought they were always on automatically and just no one ever wanted to contribute any!
haha it happens. I also didn't even know about this feature until recently
5:15 My cat actually died because of hypertrophic cardiomyopathy.
I'm still unclear on how the mechanism works to add new information. The finding and cutting is clear, but then the video jumps over the interesting part of the process.
Are you on patreon ? i think i would donate 1$ per video, your videos are interresting,
completely independant of that on a different topic, id love to see experiments like this in more detail and more complete (that is without some steps being done by others off camera, more like what Nurd rage is doing with chemistry). Either way thanks alot for all your videos i belive they do a great contribution to the education of people interrested in this stuff!
I'm not, but I'm considering launching one in the new year. I'd love to make more videos like this, with high quality animations and real experiments! What would you (and others!) be interested in seeing as patreon rewards?
I am happy to just donate, i think your videos are valuable
So we can disable aging in living people? Cool!
Not yet, no. Aging is a very, very, very complicated process. Personally I doubt we'll truly beat aging without nanotechnology; literally nanobots to repair damaged tissue, though they may use many biological processes to get the work done.
And even then, we won't have cracked clinical immortality, since just by sheer chance you can expect to be dead from some sort of accident within a thousand years or so, assuming you could live indefinitely.
UnkieRich wrote "You have been exposed to the wrong YEC crowd! I promise you that someone can be a person of faith and love science."
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Where in my comment do you see me say that religious people cannot love science?
Thanks for showing everyone what religion does to the mind:
it causes you to see things ... that aren't there.