say you're using a 60X objective with a wide field, would binning decrease the resolution? Pixel size w/o binning is probably going to be in the neighborhood of 125nm or so depending on the configuration, and binned pixels will be at least double that, what do you recommend?
Optimal pixel size depends on the numerical aperture of the objective lens (in addition to magnification - there is a microcourse on numerical aperture if you aren't familiar with it) and what you are trying to image (ie, diffraction-limited objects would require a different pixel size than whole cells), so there isn't a standard recommendation. I recommend you post the question on our microscopy discussion forum at forum.microlist.org - it's quite active so you should get some answers there!
Excellent videos! Short and sweet with appropriate visuals to help understanding. Please do more of these! :D
What a brilliant lecture. Thank you.
Very good Job explaining! This should be higher up in the search list.
Again..your videos are just amazingly explained so clearly
Thank you so much 😀
Another top quality vid.
This was great! Could you please post a video on Fourier transform?
Thanks! It's on the list of future videos. In the meantime, Bo Huang's video in the iBiology microscopy lecture series is very good.
@@Microcourses thank you! I will check it out
The curve at 1:30 under noise was used in today's online workshop over Zoom!
Good eye Tao! Talley made those slides, and we all use them. :)
Thank you for your videos
say you're using a 60X objective with a wide field, would binning decrease the resolution? Pixel size w/o binning is probably going to be in the neighborhood of 125nm or so depending on the configuration, and binned pixels will be at least double that, what do you recommend?
Optimal pixel size depends on the numerical aperture of the objective lens (in addition to magnification - there is a microcourse on numerical aperture if you aren't familiar with it) and what you are trying to image (ie, diffraction-limited objects would require a different pixel size than whole cells), so there isn't a standard recommendation. I recommend you post the question on our microscopy discussion forum at forum.microlist.org - it's quite active so you should get some answers there!
awesome, thank you :]
Thank you
increase the subject illumination!!!
Sure, with the trade-off of increased photobleaching, and phototoxicity in live samples.
🦉