The Point Spread Function
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- Опубликовано: 1 июл 2024
- If you want to understand microscopy, you gotta understand the PSF. The PSF is the basis for many practical and theoretical concepts in light microscopy, both basic and advanced.
Movies focusing on airy disk and 3D reconstruction of PSF were created by Talley Lambert.
Images of cells expressing GFP-tubulin by Talley Lambert.
Image of Abbe statue and diffraction gifs are from Wikipedia.
French subtitles were provided by BioImaging North America.
0:00 Intro
0:24 Definition of resolution
1:39 Close look at a PSF
3:40 PSF results from diffraction & interference
6:16 Diffraction in the microscope
7:09 The effect of the PSF on your images
7:42 Resolution equations
8:54 Rayleigh criterion
9:47 Size of PSF
10:13 Convolution 
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Couldnt ask for a more clearer explanation than this, you made my day. Thank you so much!!!
Fantastic video. The visuals are very well made!
Also appreciated the image of the Abbe memorial. I see it almost every day when I walk to uni. :D
One the best lectures of PSF
Thank you very much for this video! It was very informative and I learnt how the PSF relates to the Rayleigh resolution limit!
Rightly said an expert can make it simple!! Muchas gracias!
The best explanation on PSF. Completely agree with all the comments below. Thank you very much
wonderfully explained! Great narrating too!
This is by far one of the bests videos I have ever seen.
this is an amazing explanation about PSF. Thank you very much!
One of the best videos about PSF out there!
Best explantion out there. Congratulations.
amazing video. I can't wait for future courses
Very clear explanation, thanks.
Thank you so much for this informative video!
Besides the visual stress felt from staring at the bright circular light shown in the initial half of the video, it was an amazing explanation on point spread function.
Lovely to see the pink heart picture flashing out when doing the axial scanning (around 3'30") and she says "the PSF is beautiful!".
Thank you for your great explanation!
Excellent illustrations of PSF
Thank you so much for this video! Really helped me understand PSF without having to read pages and pages of writing!! You deserve more subscribers.
Glad it was helpful!
Fantastic.. I just taught a PG class on PSF.. This helped me a lot to prepare materials.
Incredible video! Really helped me out with my microscopy class
Great to hear!
I seriously applaud these videos. They are so thoughtfully put together and present the information in the clearest way possible. I can only hope that every instructional video out there some day explains things in an equally engaging, informative, and simple format. Well done!
Wow, thank you! I'm so glad you find them helpful.
you were able to make me understand these concepts with a 12 minute video
no body can explain like this thank you very much
Pretty neat explanation! What is missing for a nerd like me are some remarks on the differences between coherent and incoherent imaging and Abbe theory of image formation. What was really enlightening to me was when I read the treatment of Goodman on Fourier Optics that shows that in the coherent case, Abbe theory based on the collection of diffraction orders from the object interfering in the image plane and the convolution approach using the Amplitude PSF are equivalent, because the amplitude transfer function involves a second fourier transform of the Airy Disc PSF that gives a circ while the incoherent case (e.g. from a self-luminous object) yields the autocorrelation of the aperture function because of the square operation in the spatial frequency domain (convolution theorem).
Thank you for the brilliant lecture
Glad you liked it!
thank you for this amazing content :)
amazing video
PSF? More like "Perfect videos; you're the best!"
excellent video, damn!
excellent!
really useful
Now I get the whole point of interference and diffraction in microscope!
Thank you ♡
I've never come across lateral and axial in these waves...
Thanks...
how does enhanced resolution work (e.g. closing a pinhole a little bit)?
Hello. I found your video very explanatory, since I'm currently doing a presentation about PSF and diffraction limit for my University. Can I use some of your visuals for the presentation, and if so how can I give reference?
PS sorry for poor english
Yes, please do! You can reference the channel URL: RUclips.com/microcourses.
@@Microcourses although I finished the presentation , thank you, for giving me the permission and for helping me understand the terms
Nice video. However, two slits in front of a classical wavefront demonstrating interference is not the double-slit experiment; that refers specifically to the self-interference of a single particle's encountering a double-slit.
So the PSF is essentially the impulse response of the imaging system?
Are all fluorescence emissions coherence to make an interference pattern at the image plane?
Fluorescence emitted from a diffraction limited spot is partially coherent, and will therefore result in an interference pattern.
Just a question. What wavelength is being spoken about here? It is the emission wavelength of the green light right?
Yes, emission wavelength.
Thanks for your great vedio about PSF,do you have another vedio about OTF?
Not yet, but it's on our (long!) to do list.
Well I’ll just watch this 20 more times until my dumb brain catches on.
This is an exceptional video, but the diatom at 1:00 is certainly not Pleurosigma angulatum. Not trying to nit-pick or act smug, this is one of the best PSF videos I have watched so far.
Thank you! My records may be incorrect. What species is it?
@@Microcourses I think it should be Frustulia rhomboides.
By the way, it is one MAXIMUM and two or more MAXIMA ;-) @ 3:53 And this really is 2x the amplitude ;-(
Stop the vocal fry please
I am very curious if you ever commented that under a video of a male speaker...