Thank you for this tutorial. How many cells do you load in your tube to be sorted, and in what volume? Also, how do you select a proper flow rate? Thanks.
Thanks for the question! We generally try keep sorting volumes under 1mL in my facility with at max cell concentration of 10^7 cells/mL (this can change greatly depending on the cells type). Selecting a proper flow rate is a balance between the sort efficiency and the sort timing- you don't want a low efficiency as then you are losing a lot of cells but you also don't want a long time for the sort as the cells then start to die. Generally we try maintain an efficiency of at least 85%.
Thank you!
Thanks and appreciate .......
Thank you for this tutorial. How many cells do you load in your tube to be sorted, and in what volume? Also, how do you select a proper flow rate?
Thanks.
Thanks for the question! We generally try keep sorting volumes under 1mL in my facility with at max cell concentration of 10^7 cells/mL (this can change greatly depending on the cells type). Selecting a proper flow rate is a balance between the sort efficiency and the sort timing- you don't want a low efficiency as then you are losing a lot of cells but you also don't want a long time for the sort as the cells then start to die. Generally we try maintain an efficiency of at least 85%.
Do we use fluorochromes every time in flow cytometry?
No you do not- it is commonly done that way but is not necessary.
Hi,
Is the their any book you can recommend for the preparation of ASCP SCYM exam