Hey, loved this series about plasmid design. I didn't quite grasp the concept of eukaryote promoters though... Could you maybe make a seperate more in depth video about those? would be greatly appreciated, coming from an aspiring biologist!
so do I, I think the concept of the eukaryote promoters is difficult for me. Such many upstream factors. I want to learn more detailed information, Thanks!
Viruses are a lot more complicated! Some viral genes are expressed using a viral RNA polymerase, while other viruses exploit their host cells (either bacterial or eukaryotic) and express their genes using a host polymerase. It depends what virus you are interested as how it's promoter works/is structured. There is therefore a lot of variation in bacterial promoter structure and more than I can easily capture in a 15min video!
@@katharinehubbard5043 Thanks for your kind feedback. Let's say "cassava mosaic virus". It will be highly appreciated If you could share some good references with us, Thanks in advance
@@abdoulayeassane2852 I've not worked on Cassava Mosaic Virus so am not an expert here! However, Cassava Mosaic Virus is a geminivirus which has a single stranded DNA genome. Like a lot of DNA viruses, geminiviruses don't encode their own RNA polymerase, but rely on the host (eukaryotic) polymerase for transcription. Because the genes are transcribed with a eukaryotic nuclear RNA polymerase, they have a similar promoter structure to eukaryotic genes - Figure 4 of this paper shows the presence of TATA box motifs in regulatory regions. Hope that helps! www.ncbi.nlm.nih.gov/pmc/articles/PMC6640248/
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This is really neat. I am determined to learn this molecular biology. Thanks for the upload.
Muchas gracias profesora por explicar de forma tan clara. You're the best
Please keep going you are gorgeous. Many thanks goes to to from form UEA Norwich!!!
I love you!
Hey, loved this series about plasmid design. I didn't quite grasp the concept of eukaryote promoters though... Could you maybe make a seperate more in depth video about those? would be greatly appreciated, coming from an aspiring biologist!
Thanks - eukaryotic promoters are on my list to do!
@@katharinehubbard5043 thankss
so do I, I think the concept of the eukaryote promoters is difficult for me. Such many upstream factors. I want to learn more detailed information, Thanks!
How about promoter for viruses?
Viruses are a lot more complicated! Some viral genes are expressed using a viral RNA polymerase, while other viruses exploit their host cells (either bacterial or eukaryotic) and express their genes using a host polymerase. It depends what virus you are interested as how it's promoter works/is structured. There is therefore a lot of variation in bacterial promoter structure and more than I can easily capture in a 15min video!
@@katharinehubbard5043 Thanks for your kind feedback. Let's say "cassava mosaic virus". It will be highly appreciated If you could share some good references with us, Thanks in advance
@@abdoulayeassane2852 I've not worked on Cassava Mosaic Virus so am not an expert here! However, Cassava Mosaic Virus is a geminivirus which has a single stranded DNA genome. Like a lot of DNA viruses, geminiviruses don't encode their own RNA polymerase, but rely on the host (eukaryotic) polymerase for transcription. Because the genes are transcribed with a eukaryotic nuclear RNA polymerase, they have a similar promoter structure to eukaryotic genes - Figure 4 of this paper shows the presence of TATA box motifs in regulatory regions. Hope that helps! www.ncbi.nlm.nih.gov/pmc/articles/PMC6640248/
@@katharinehubbard5043 Thanks for your kind feedback