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shouldn't the primer be complementary to the gene of interest?@11:43

Thank you🙏

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This is going to save my biochemistry grade

YOURE AMAZING!!! Godsend 💕💓 beautiful explanation, perfectly understood!

What was the use of the 255 number in the normalisation formula?

Thanks very much.

Amazing video thank you very much

Thank you so much that was so very well explained and straight to the point.

Thats perfect!!!

Truly well done, thank you for your insight!

Amazing, thank you so much!!!

how to design primers if we want to add a tag to protein?

My 6th grader asked me about Spectrophotometer, "what does it do and what is it used for?" Aside from telling him, ''It is a light-related instrument'' - I didn't have much to say about it. What better feedback when he returns from school😊. No School like RUclips, Thanks Katharine Hubbard.

TY SM , DOING GODS WORK

Thank you for this great video! Very informative!

Hello from România, i havent 110 apple tree with agrobacterium tumefaciens, i remove all trees , after how much years i cant plant apple tree in that soil near 2meter to the tree how have agrobacterium. Thanks a lot. And how i cant clean the soil...thanks...a lot

Awesome explanation 🎉

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:)

Great tutorial! And about the volume of the reaction? Do you think we should consider it?

Please can you tell the tool to design this type of primers?

Thank you so much ma'am, this video really helped me a lot :)

Thankyoy

This is Varsity stuff but How i wish RUclips was around when I was in high school 😢

Here is a general structure of a primer combining these components: 5' - Leading Sequence - Restriction Site - Gene-Specific Sequence - 3' When designing primers for cloning, amplification, or mutagenesis, it's important to include specific sequences that ensure the primer's proper function. Below are the key components to consider when designing primers for these purposes: The primer above includes: Leading sequence: GCG (3 bases to enhance restriction enzyme efficiency) Restriction site: GAATTC (EcoRI) Gene-specific sequence: ATGACTGACTGACTGAC

Thank you very much for your videos! They are very well structured and helpful (from a chemist who learns bio)

very nice , can you please help tell , can i use this for Gems Spectrometer

I guess after watching this video I just HAVE TO subscribe to this channel

You are adding in the reverse primer (when you write It 3'-5') the sequence of the MCS in 3'-5' but that sequence that you added is actually the MCS written in 5'-3' (i ve searched the sequence). So, do you have to change the order of the MCS into 3'-5', dont you?

AMAZING.. WONDERFUL CLEAR EXPLANATION

Just imagine if a cell had a cloraplast and a mitochondria. They would be passing oxygen atoms back and forth.

Incredible video thank you so much !!!

You are my hero!

You are a life saver. You explanation made it look like a simple thing to know.

Can I just use test tube instead of quivet

Did bacteriophages evolved not to have those palindromic sequences?

Can you please explain how to choose the wavelength in the spectrophotometer for calculating absorbance

I Lov it

Thank you for sharing! Very informative! You're such an effective educator!

Amazing 😍

Hello, I'm writing a lab report involving chlorophyll determination but I'm having trouble finding a study that uses these exact conversion factors and formulas. Could you please send me the link of where it was taken from? Thanks!

When an expert teaches something, life becomes easy. Thank you!

This video is gonna save my live in genetic engineering. Thank you!

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How it g/L equivalent to microgram/mL. Isn't it mg/L?

Thank you so much! Please could I ask how you would estimate an Amax value based on the experimental data?

very good explanation,watched about 5min and then I know this is what I needed. Also saved another 2 or 3 vedios for my paper (pharmacology😢) Many thanks

one in all, everything we must know is here in the video, I appreciate you <3

Great video. Thanks.