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This video is gonna save my live in genetic engineering. Thank you!

that perfect as hell you must get much much mre mybe if you make playlist according to lippincot or harper , will be ammzing

How it g/L equivalent to microgram/mL. Isn't it mg/L?

Thank you so much! Please could I ask how you would estimate an Amax value based on the experimental data?

very good explanation,watched about 5min and then I know this is what I needed. Also saved another 2 or 3 vedios for my paper (pharmacology😢) Many thanks

one in all, everything we must know is here in the video, I appreciate you <3

Great video. Thanks.

Katharine.God bless your mother

Nice

you saved me with this amazing video: clear and complete! <3

Thanks for sharing these videos for free, it's super helpful for Biotechnology section in my National Biology Olympiad!

thank you so much！Your explanation about the stop codon really helps me a lot with my assignment.❤

thanks mam, you save my money and time. God bless you. No one had explain the STOP CODON properly

It is not completely clear how you bring the second image with the scale at the bottom?

How is no one talking about her perfect arrows??? Great video btw

A lovely video ❤ however there's something i don't understand, in nested pcr, a primer amplifies a specific region in a sequence but i don't understand how, since a Primer is supposed to amplify from the binding site to the end no?

Hiya , I’ve a question. Is agrobacterium possibly why some get morgellons leisions ? Or unlikely ? Can this bacteria copy dna of insects? Thanks 🙏

Excellent video. Very, very well explained.

This is exactly good lecture

15:43 Antibiotic resistant- why would you want to lower the plants natural defense & let a mimic run around, Like sneaking your buddy into the strip club (he's good, because he has escorted service 😂)

Because agrobacterium has BSL level 1. Implies it is man made! Only native plants (parent plants are symbiotic to the environment & thus do not contain any evidence of agrobacterium in the soil or roots because it is man made.

8:36 Lies The plant knows it is foriegn. This is observed in vito, because the m f mimic/ replication does not contain the same information the host recognizes this as a deletion. & this is where you get a certain percentage of abnormalities and mutations in your lineages & offspring. It is through the host. Because you just created it's own secretory pathway to allow the impermeable membrane to allow it to secrete the foriegn genomes it was designed to keep out! 🤦‍♂️ Like giving your loved ones abnormalities and mutations because you didn't know better because someone said it was safe. Do you really believe you're helping to create less dis-ease / disease & illnesses, abnormalities and mutations?

Simplified, straightforward approach, thanks:)

6:55 I've read organisms engulfing others like that is rare but was recently observed and the description was published in the journals _Cell_ and _Science_ .

Hi is there wobble in the recognition of the restriction site

Thank you very much, a very straightforward explanation. I have a question: what happens if the designed primers have very different Tm between them. Is it possible to design the primers before or after the gene sequence to avoid this and also get better CG percentages? If not, is there another approach to increase the PCR efficiency if the gene sequence only allows to design a non compatible pair of primers?

Hey! If I am thinking of transcribing my plasmid into an mRNA, do I use the entire gene of interest? Or do I try to find the mRNA sequence and use the cDNA of that instead to put into my plasmid?

Great Video!

I am curious if this really gives consistent results. I had the impression that modern mobile phones do all kinds of non-linear "corrections" attempting to give the most pleasing image, so the absolute R,G,B pixel values are not necessarily directly related to just that one point on the image, but everything else in the image too, and possibly even the recent history of image frames as well, if the phone is attempting to compensate for what it believes is the currently-present ambient lighting?

thank you

I have a problem with your use of two different restriction enzymes to cut the palsmid and gene of interest, won't this affect the joining of sticky ends through hydrogen bonding before they are ligated?

Shukran, asante sana

thank you so much!! brilliant explanation with drawings. you saved my time !! <3 <3 <3

At 3.01 how is the bond broken????

Thank you so much for your clear explanation!

Thank you for your video! It saved me a lot when I started my new experiments using plasmids!

How do molecular machines like triple-A ATPases work? It is known that they do work upon the hydrolysis of ATP+H2O to ADP+Pi, but how is this converted into mechanical energy? And how comparable are these -30 kJ mol-1 compared to the constant random noise of the environment these molecular machines are in?

Awesome - this is great thank you Katherine :)

Awesome lecture, thanks for simplifying a complicated subject

hbb147-honey

Can i know the matters which can be measured by it and the matters can't

Great job 👍

Why you speaking so fast😭

Hi, the reverse primer should be 5-CTCGAG or 5-GAGCTC? I am confused now😅

Amazing video, thank you!

what is the purpose of adding additional nucleotides at the end ?

This helped SO MUCH I was going mad over my lecture slides but this was amazing. Thanks a lot

Is epsilon the same as wavelength?

amazing video! thanks!

You are incredible, you have fantastic teaching skills! I was finally able to understand the entire process, even though I had been doing it for months, reading articles and everything, I was only now able to make the necessary connections.