Video is informative but wanted to bring to your notice that , In competitive ELISA - no Color means positive(I.e no antigen is free to bind the enzyme coated antibody) so the added enz. Coated antibody are washed and on addition of the substrate no Color produced . If Color is produced it means antibody are not present in the pt. Sera I.e on added enz. Coated antibody are bound to antigen on plate and on addition of the substrate Color produced.
Sbko english ka 14 banna h🤦♀️🤦♀️ Bhai atlst hindi m agar sahi se explain kr skte ho to please explain it.. Hindi and english doesn't matter if you have good communication skills.. English just a language please don't do. That
A sandwich ELISA is more sensitive and robust as the antibody binds to two sites on the antigen. This increases the binding specificity of the primary capture antibody to the antigen as well as the binding specificity of the detection antibody to the antigen.
Please recheck your explanation of competitive elisa. As the second antibody is of the same type of the first one, so it will not react and no color will be produced. No color = positive result
Substrates are 1) Alkaline phosphate - Paranitrophenyl phosphate (blue clr) 2) Horseradish peroxidase - tetramethyl benzedene (yellow clr) The buffer solution is used to wash
What are the reasons to opt for a specific type of ELISA test? The direct ELISA and competitive ELISA seemed very similar, so when do you use which one?
Secondary antibodies provide signal detection and amplification along with extending the utility of an antibody through conjugation to proteins. ... Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody. I hope this helps
Video is informative but wanted to bring to your notice that , In competitive ELISA - no Color means positive(I.e no antigen is free to bind the enzyme coated antibody) so the added enz. Coated antibody are washed and on addition of the substrate no Color produced . If Color is produced it means antibody are not present in the pt. Sera I.e on added enz. Coated antibody are bound to antigen on plate and on addition of the substrate Color produced.
Broo...first explain why we do it
bad language
I thought of skipping this topic but now I understood this so well with crystal clarity✨ because of your teaching.🥰🥰 Thank you so much sir❤️
Sbko english ka 14 banna h🤦♀️🤦♀️
Bhai atlst hindi m agar sahi se explain kr skte ho to please explain it.. Hindi and english doesn't matter if you have good communication skills.. English just a language please don't do. That
I really appreciate you for creating such a great content ❤️ keep uploading 🔥
Why Sandwich ELISA is more sensitive?
A sandwich ELISA is more sensitive and robust as the antibody binds to two sites on the antigen. This increases the binding specificity of the primary capture antibody to the antigen as well as the binding specificity of the detection antibody to the antigen.
Thank you Sir
Please recheck your explanation of competitive elisa. As the second antibody is of the same type of the first one, so it will not react and no color will be produced. No color = positive result
Agreed, if Color produce in Competitive ELISA it means the results is negative while on the other hand No color production gives Positive Results
Competitive and indirect Elisa looks the same ?
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Awsome video... Thanku soo much sir🙂
Very nyc
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Please clear your concept too many mistakes
You are not mentioned in any substrate name similarly not washing agents..🥺
Substrates are
1) Alkaline phosphate - Paranitrophenyl phosphate (blue clr)
2) Horseradish peroxidase - tetramethyl benzedene (yellow clr)
The buffer solution is used to wash
no colour formation in competitive elisa (reference cp baveja)
thanks, bro really helpful nice explanation...
How can i listen this video in urdu?
First of all thanks for uploading video....simple n clear cut explaination
Competitive elisa 8:24
Easy and simple... Very good explanation thnk u
Why don't you tell the concept🤦🤦👎👎
Very helpful and the explanation is clear, thank you so mucj
Nice content
Am rushing towards my exams but this video is super ❤❤❤ thank you Sir for the video
thank u so much
Tnku Tnku so much sir...es topic ko easy bnane ke liye nhi to mujhe ye topic pta nhi kya lg rha tha 😤😤😤😤
Thanks sir
Thanku sir it's really helpful to me
❤👍🏻
❤
more helpful easily understand tq so much 🥰
Thanks. It is really helpful. Have zero knowledge about it but after watching this video. I can explain it in my own words ❤
Thanks
Osmmm
Fully explained thanku sir
Thank u for sharing this video..😊😘👍👍👍
In competitive appearance of colour indicates -ive test
Very informative video...Thank you very much 🙏
What are the reasons to opt for a specific type of ELISA test? The direct ELISA and competitive ELISA seemed very similar, so when do you use which one?
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@Chris Allen glad I could help xD
Thank you for making it simple
Thank you!!!! this was such a great intro explanation
you saved me THANK YOU great explanation
Very helpful video ☺️
I Really appreciate you thanks a lot
Today is my exam thank u so much u made my day 🙏
Thank you for clear my doubts
This is helpful. Thank you
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Helpful video 👍
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Where is reverse ELISA?
Bro you can add notes also along with this so that it will be more useful 🙏
Helpful content
Thank you sir ❤️
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Thank you.
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👍
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Why we use secondary antibody ??
Secondary antibodies provide signal detection and amplification along with extending the utility of an antibody through conjugation to proteins. ... Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody.
I hope this helps
Nice explanation
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Very helpfull
Nice explanation, thanks for the lecture
You explained it really well.. thank you 😊
Thank you for making it simple ❤️