ELISA Test : All types with Mechanism discussed in details : Microbiology

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  • Опубликовано: 29 сен 2024

Комментарии • 103

  • @neerajchhimwal359
    @neerajchhimwal359 2 года назад +34

    Video is informative but wanted to bring to your notice that , In competitive ELISA - no Color means positive(I.e no antigen is free to bind the enzyme coated antibody) so the added enz. Coated antibody are washed and on addition of the substrate no Color produced . If Color is produced it means antibody are not present in the pt. Sera I.e on added enz. Coated antibody are bound to antigen on plate and on addition of the substrate Color produced.

  • @DrMSSR_tips_tricks
    @DrMSSR_tips_tricks Год назад +1

    Broo...first explain why we do it

  • @alshammary94
    @alshammary94 Год назад

    bad language

  • @harshukutty4350
    @harshukutty4350 2 года назад +19

    I thought of skipping this topic but now I understood this so well with crystal clarity✨ because of your teaching.🥰🥰 Thank you so much sir❤️

  • @sadafchaudhary6063
    @sadafchaudhary6063 Год назад +1

    Sbko english ka 14 banna h🤦‍♀️🤦‍♀️
    Bhai atlst hindi m agar sahi se explain kr skte ho to please explain it.. Hindi and english doesn't matter if you have good communication skills.. English just a language please don't do. That

  • @merishaacharya2257
    @merishaacharya2257 3 года назад +14

    I really appreciate you for creating such a great content ❤️ keep uploading 🔥

  • @soumighosh9075
    @soumighosh9075 3 года назад +2

    Why Sandwich ELISA is more sensitive?

    • @afreenlirani
      @afreenlirani 3 года назад

      A sandwich ELISA is more sensitive and robust as the antibody binds to two sites on the antigen. This increases the binding specificity of the primary capture antibody to the antigen as well as the binding specificity of the detection antibody to the antigen.

    • @soumighosh9075
      @soumighosh9075 3 года назад +1

      Thank you Sir

  • @ujjalmandal8061
    @ujjalmandal8061 4 года назад +11

    Please recheck your explanation of competitive elisa. As the second antibody is of the same type of the first one, so it will not react and no color will be produced. No color = positive result

    • @POChinkiGaming
      @POChinkiGaming 2 года назад

      Agreed, if Color produce in Competitive ELISA it means the results is negative while on the other hand No color production gives Positive Results

  • @merlinjohn7381
    @merlinjohn7381 Год назад +1

    Competitive and indirect Elisa looks the same ?

  • @umairkhan-gy3bc
    @umairkhan-gy3bc Год назад +1

    Thank you so much sir , Your way of teaching is very good ... I have subscribed your channel right now

  • @fatimabushra1758
    @fatimabushra1758 2 года назад +2

    Awsome video... Thanku soo much sir🙂

  • @Spiritualworld1122
    @Spiritualworld1122 3 года назад +1

    Very nyc

  • @sunkireddymonika8747
    @sunkireddymonika8747 2 года назад +1

    Ultimate vedio , very good explaination 👌👌👌.. Really ur explanation was sooo good👍

  • @WKTVlogs
    @WKTVlogs Месяц назад

    Please clear your concept too many mistakes

  • @surajwanjari5826
    @surajwanjari5826 8 месяцев назад

    You are not mentioned in any substrate name similarly not washing agents..🥺

    • @IrisRim
      @IrisRim 5 месяцев назад

      Substrates are
      1) Alkaline phosphate - Paranitrophenyl phosphate (blue clr)
      2) Horseradish peroxidase - tetramethyl benzedene (yellow clr)
      The buffer solution is used to wash

  • @cubexycgaming7797
    @cubexycgaming7797 3 года назад +1

    no colour formation in competitive elisa (reference cp baveja)

  • @vigneshd6735
    @vigneshd6735 3 года назад +2

    thanks, bro really helpful nice explanation...

  • @farhankhan8197
    @farhankhan8197 9 месяцев назад

    How can i listen this video in urdu?

  • @leenaevangeline1231
    @leenaevangeline1231 2 года назад +1

    First of all thanks for uploading video....simple n clear cut explaination

  • @mohitpatel209
    @mohitpatel209 3 года назад +2

    Competitive elisa 8:24

  • @nehak5457
    @nehak5457 4 года назад +2

    Easy and simple... Very good explanation thnk u

  • @riseinwise5202
    @riseinwise5202 3 года назад +1

    Why don't you tell the concept🤦🤦👎👎

  • @vimbainashemakaha9231
    @vimbainashemakaha9231 3 года назад +1

    Very helpful and the explanation is clear, thank you so mucj

  • @vivekkumarprajapati1782
    @vivekkumarprajapati1782 23 дня назад

    Nice content

  • @mrmc55
    @mrmc55 9 месяцев назад

    Am rushing towards my exams but this video is super ❤❤❤ thank you Sir for the video

  • @ZinIsMe-yq8ih
    @ZinIsMe-yq8ih 5 месяцев назад

    thank u so much

  • @kaurshsidhu5417
    @kaurshsidhu5417 2 года назад

    Tnku Tnku so much sir...es topic ko easy bnane ke liye nhi to mujhe ye topic pta nhi kya lg rha tha 😤😤😤😤

  • @sami6169
    @sami6169 Год назад

    Thanks sir

  • @babitasingh3703
    @babitasingh3703 3 года назад +1

    Thanku sir it's really helpful to me

  • @rabiyashajahan6287
    @rabiyashajahan6287 Год назад

    ❤👍🏻

  • @sami6169
    @sami6169 Год назад

  • @shivanithakur2168
    @shivanithakur2168 3 года назад +1

    more helpful easily understand tq so much 🥰

  • @junaidbabar8446
    @junaidbabar8446 Год назад

    Thanks. It is really helpful. Have zero knowledge about it but after watching this video. I can explain it in my own words ❤

  • @zaid9878
    @zaid9878 2 года назад

    Thanks

  • @sayimamukhtar2626
    @sayimamukhtar2626 Год назад

    Osmmm

  • @Anonymous.128
    @Anonymous.128 3 года назад +1

    Fully explained thanku sir

  • @Megha-ij8eo
    @Megha-ij8eo 3 года назад

    Thank u for sharing this video..😊😘👍👍👍

  • @MuneebMagray
    @MuneebMagray 2 года назад

    In competitive appearance of colour indicates -ive test

  • @vetprofessional1656
    @vetprofessional1656 2 года назад

    Very informative video...Thank you very much 🙏

  • @gerriekeuning4523
    @gerriekeuning4523 3 года назад +7

    What are the reasons to opt for a specific type of ELISA test? The direct ELISA and competitive ELISA seemed very similar, so when do you use which one?

    • @chrisallen5025
      @chrisallen5025 3 года назад

      i realize it's pretty randomly asking but does anyone know of a good website to watch newly released movies online?

    • @jakobfinn4339
      @jakobfinn4339 3 года назад

      @Chris Allen I would suggest flixzone. Just google for it :)

    • @aydenjackson8328
      @aydenjackson8328 3 года назад

      @Jakob Finn yup, been watching on flixzone for since march myself :D

    • @chrisallen5025
      @chrisallen5025 3 года назад

      @Jakob Finn Thanks, I signed up and it seems like they got a lot of movies there :) I really appreciate it !!

    • @jakobfinn4339
      @jakobfinn4339 3 года назад

      @Chris Allen glad I could help xD

  • @aparnaaparna904
    @aparnaaparna904 Год назад

    Thank you for making it simple

  • @elianamartos2992
    @elianamartos2992 Год назад

    Thank you!!!! this was such a great intro explanation

  • @bananali2676
    @bananali2676 3 года назад

    you saved me THANK YOU great explanation

  • @arthiindira6925
    @arthiindira6925 4 месяца назад

    Very helpful video ☺️

  • @bereketbekele4918
    @bereketbekele4918 2 года назад

    I Really appreciate you thanks a lot

  • @nishamandal1331
    @nishamandal1331 2 года назад

    Today is my exam thank u so much u made my day 🙏

  • @varshasenthilkumar1541
    @varshasenthilkumar1541 2 года назад

    Thank you for clear my doubts

  • @christogonusekenwaneze24
    @christogonusekenwaneze24 Год назад

    This is helpful. Thank you

  • @arajeshwari914
    @arajeshwari914 Год назад

    Nice very good helpful

  • @DrMSSR_tips_tricks
    @DrMSSR_tips_tricks Год назад

    Directly jumped into topic

  • @ts6911
    @ts6911 2 года назад

    Really really thanks for this

  • @BRIGHTSWAIN
    @BRIGHTSWAIN 2 года назад

    Thank you sir

  • @ismetarakhatun9024
    @ismetarakhatun9024 2 года назад

    Thank u so। Much 💙

  • @reshmi.g3590
    @reshmi.g3590 2 года назад

    Very use full vedio

  • @krishnendhuraj3166
    @krishnendhuraj3166 Год назад

    Helpful video 👍

  • @chirudeepu1980
    @chirudeepu1980 2 года назад

    Thanks for this ❤

  • @ved3113
    @ved3113 3 года назад

    Where is reverse ELISA?

  • @sumeshsudhakaran4823
    @sumeshsudhakaran4823 3 года назад

    Bro you can add notes also along with this so that it will be more useful 🙏

  • @Deep_blue49
    @Deep_blue49 Год назад

    Helpful content

  • @S...n123-o3p
    @S...n123-o3p 2 года назад

    Thank you sir ❤️

  • @sambashiva1988
    @sambashiva1988 3 года назад

    Very nice explanation 👌

  • @kalyani5888
    @kalyani5888 2 года назад

    Thank you sir

  • @poojitha4158
    @poojitha4158 2 года назад

    Nice explanation 🤗🤗👏tqq:)

  • @AmitisL
    @AmitisL 3 года назад

    very helpful , thank you

  • @prajwalareddy2603
    @prajwalareddy2603 3 года назад

    Thank u so much Sir

  • @mohanm8530
    @mohanm8530 3 года назад

    Very very helpful tnq

  • @pujaa__21
    @pujaa__21 2 года назад

    Excellent explained ....👍 Thank you

  • @sanjaisrao484
    @sanjaisrao484 3 года назад

    Exelent thanks

  • @creativity_with_Nomi
    @creativity_with_Nomi 2 года назад

    Awesome

  • @mandasweshika7935
    @mandasweshika7935 3 года назад

    Very easy for learning thank you

  • @akashreshmi1092
    @akashreshmi1092 3 года назад

    I like it bro

  • @niranjaniyeggidi7038
    @niranjaniyeggidi7038 3 года назад

    Thank u sir

  • @arundart9395
    @arundart9395 2 года назад

    Thnx👍🏻

  • @kellibryant707
    @kellibryant707 3 года назад

    Thank you.

  • @Mittalnayan04
    @Mittalnayan04 3 года назад

    Thankyou !

  • @mahmoudaldeeb452
    @mahmoudaldeeb452 3 года назад

    Thanks man

  • @jasminflower9762
    @jasminflower9762 3 года назад

    Thanks

  • @souravcreativity..590
    @souravcreativity..590 3 года назад

    Amazing

  • @fouziapp
    @fouziapp 2 года назад

    👍

  • @dharitreesonowal8343
    @dharitreesonowal8343 3 года назад

    Thank you

  • @sakshichaudhary260
    @sakshichaudhary260 4 года назад

    Very helpful vedio
    Thnx sir

  • @nandanimalhotra1461
    @nandanimalhotra1461 3 года назад

    Helpful

  • @Imran-tv7ic
    @Imran-tv7ic 3 года назад

    Supeb sir

  • @imranrasheed.793
    @imranrasheed.793 2 года назад

    wah maza hi agya

  • @implyunil2937
    @implyunil2937 3 года назад

    Why we use secondary antibody ??

    • @afreenlirani
      @afreenlirani 3 года назад

      Secondary antibodies provide signal detection and amplification along with extending the utility of an antibody through conjugation to proteins. ... Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody.
      I hope this helps

  • @sakshichaudhary260
    @sakshichaudhary260 4 года назад

    Nice explanation

  • @hajarqaireine6048
    @hajarqaireine6048 3 года назад

    thank you so much, Dr !

  • @kheiramakreloufi6209
    @kheiramakreloufi6209 4 года назад

    very well explained thank you so much

  • @hruaiichhangte626
    @hruaiichhangte626 4 года назад

    Very helpfull

  • @RohitPrasad-ep5wu
    @RohitPrasad-ep5wu 4 года назад +1

    Nice explanation, thanks for the lecture

  • @abhishekbaranwal2006
    @abhishekbaranwal2006 2 года назад

    You explained it really well.. thank you 😊

  • @anaghar2354
    @anaghar2354 2 года назад

    Thank you for making it simple ❤️