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It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
Thank you Sir! Very helpful that you included the case where the primary antibodies aren't specific to the antigens to see the comparison.
THANK YOU SO MUCH! Your video was fast, simple, and informational. I appreciate you.
You explained the toughest part in simplest way.hats off sir
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is valuable for us. Paytm/Google pay number 8830061003.
best ever explained...thank you for explaining it so well.
The Best Ever Explanation One can Get. Precise
A really precise and pedagogical explanation. Well done.
You’re amazing! Thank you so much :)
what would you say the ease and speed of the process is? how long does it take to perform indirect elisa?
Your lectures contains more amount of data , your vocals are easy for us to understand ..... 💓
It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
Thank you from Cameroon 🇨🇲❤️
Best immunology video I hv ever seen. .great job ..😊😊😊
Excellent explanation! Thank you
well the way you teaching is awesome great work buddy keep going.....
i would like to add a question that
after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation??
looking forward to your reply thank you
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
@@sajidmicrobiology thank you soo much buddy
The sample we are putting in the titer after the coating buffer. Is the sample just the blood of the person?
Its not whole blood... it is serum most of the time
It's well explained and it's really helpful. Thanks for the video and hope you can do more. LOVEYOURSELF ♥️
Nice presentation, very easy to understand. Thanks a lot.
Is it possible to do the opposite by putting the antibody bound to the polystyrene plate to determine the antigen concentration in the sample?
Yes
@@sajidmicrobiology ok thanks
Excellent explanation/instruction. [Antibodies is misspelled at 6:15 on the video.]
Well explained! Hats of to you sir.
Best explanation I swear the doctor who give me the lecture don’t know what to do
I red the lecture for for 6 Hours and didn’t understand what my doctor was saying
While you explain it in few minutes
You are the best♥️♥️♥️
Most welcome
best explaination and helped me before my presentation 😊
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
Thanx Mohit ... i am in the process of uploading
this topic wasn't even clear in wilson and walker but you explained it very well. thank you
Thanx.. would you like to help our channel by donating small amount (Rs 5 to 100)? This will encourage out team to make more videos
tq so much sr..concept clear n easy explanation
Best explanation. Thank you
What an explanation!
Very well-explained! 😊 👍
Awesome
veryy hardworking masahaalh keep it up sir ap electrophoresis pa bhi video upload krya
Why are we adding the acid when we already getting the color by substrate addition only
To stop the reaction
You are brilliant ...Brooklyn continue. with new video
Thanx
Very clear discussion!! Thank U Sir:'>
Well explained ..thank you.
Excellent explanation with diagrams
Assalamoalaikum. Brother! I enjoy your lecture very much. Please keep it up. May Allah livelong you. Thank you
Walekumasslam... most welcome
Well presented... excellent..
Sir the chromagen color dissolve inside the glass or produces colored light? Thanks
It dissolves
Amazing👍🏻
Thanks for the guidance
Explained very well 😊
Brilliant explanation 💚
Very nice 👍🏼
has secondary antibody gain antigen property after binding enzyme to bind primary antibody!!
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
Bro your teaching method is too good and perfect
Most welcome Joshwa .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
Initially from where we have to take antigen...?
Which are attaching to microplate surface.
Yes
Ur not understood my question.
Ooo antigen kidarse aya
Artificially availability is there of antigen
Agar apko koi antibody detect karni hain to apke pass uss antibody ka specific antigen purified form me chahiye..
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain
Just woaw
Well explained!.. Thanks.
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
Too gooddd
Very very helpful video 👏👏👏
Thanks a lot.
Very well explained.
Excellent explanation, thank you 👍😀
Thanx
This was so great
That was perfect ,tnx so much
THANK YOU SO MUCH THIS VIDEO WAS REALLY REALLY HELPRFUL ...... EXCELLENT
Most welcome
If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5)
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💞👍💞
Very nice explanation sir thanks
Thanku so much. Explanation is too good 😊
Vatsala sharma most welcome
Great
Thank you so much😊
thank u so much.... you did a great job...😊
Most welcome
Very nice indirect Elisa lecture
Very good explanation
Nice
best explanation
Sir update more videos. It is very useful to understand with animation.
Sure
thanks alot ❤
Thank you for this
Really well explained
Thanx
very nice video sir thanks 👌👌👌☺️☺️
Good explanation thanks
very nicely explained.. thank u sir.
Most welcome
Best ever
Thank u so much
Tysm sir
Is it 4 generation or third
Very well explained. Tq sir
Most welcome vijayachandra .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
Sir will you plz conduct lecture on how to take notes from textbooks
I subscribed.
Good explanation
Excellent thanks so much 😘 sir
Most welcome
Tqq so much
Thank you
Thank you ☺☺
Where is video of direct elsiaa?
Nyc sir
difference between sandwich anf this one
thank you so much this was very helpful 🤍🤍
Most welcome
Direct also plzz sir
Sure
@@sajidmicrobiology thankyou so much sir.
Awesome
Awesome