I am having an issue running Benzoic acid on a Phenomenex Luna C18 column, the peak for the benzoic acid starts eluting fine but then has a really bad tail towards the end affecting my USP tailing, what I don't understand is that my salicylic acid peak is coming out fine as I have both of those components in the same standard solution what can be causing this tailing? I reverse-flushed the column thinking the column was dirty but it didn't help.
I am having an issue running Benzoic acid on a Phenomenex Luna C18 column, the peak for the benzoic acid starts eluting fine but then has a really bad tail towards the end affecting my USP tailing, what I don't understand is that my salicylic acid peak is coming out fine as I have both of those components in the same standard solution what can be causing this tailing? I reverse-flushed the column thinking the column was dirty but it didn't help.
Thanks
I m always amazed with your explanation prof. I did have a good experience last year at Axion last during my chem 500 .
Longue life prof
love the videos, really helped me in my studies, great explanation, thanks!
1.physical problem due to instruments
2.chemical problem due to acid base reactions
Thank you, You are great
Base deactivated column C18 columns