HPLC Tubing & Fittings

🔬 Welcome to our educational series on High-Performance Liquid Chromatography (HPLC). In this episode, we delve into the intricacies of HPLC tubing and fittings, essential components in liquid chromatography systems.
What's Inside:
Stainless Steel vs. PEEK Tubing: Understand the strengths and limitations of stainless steel and PEEK (polyetheretherketone) tubing in HPLC applications. Discover why stainless steel, known for its high pressure handling, is challenging to cut, and why PEEK offers more flexibility in cutting.
Fittings Types & Techniques: Learn about the differences between stainless steel and PEEK fittings. We discuss how stainless steel fittings provide a permanent fit once swaged, and the versatility of PEEK fittings, which can be made to fit various columns without worrying about ferrule depth.
Cautions with PEEK Tubing: Explore important considerations when using PEEK tubing, especially concerning solvent compatibility and pressure ratings. Find out why PEEK is a preferred choice for many, especially in reverse-phase HPLC with common solvent combinations like methanol/water or acetonitrile/wat​er.
Cutting and Sizing Tubing: Discover tips on selecting the right diameter for PEEK tubing and techniques for achieving a perfect cut, ensuring optimal performance in your HPLC syst​​em.
Achieving Perfect HPLC Fittings with PEEK: Step-by-step guide on creating ideal fittings for HPLC systems using PEEK tubing, covering aspects like ferrule depth and ensuring a tight fit for high-pressure application​​s.
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what is the functional difference between running green stainless vs red stainless @ 0.3mL/min (Ion Paired, RP, LCMS). nice vids, thx for sharing