Tutorial : Agilent Techs High Performance Liquid Chromatography (HPLC) 1260 Infinity with DAD (HD)

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  • Опубликовано: 27 ноя 2024

Комментарии • 88

  • @denniskiprotich603
    @denniskiprotich603 Год назад +6

    This has been a super clear stepwise guide. Just as if I am there running the test. Thank you.

  • @LabBrat8
    @LabBrat8 2 года назад +7

    Thank you for the well edited footage and showing every step in running a sample with one method in the sequence.
    Our HPLC just came in this week! The maintenance technician said 100mcL was the max injection and did not mention a need to filter samples or solvents. We will follow these protocols as we get comfortable with the machine :)

  • @saminariaz9052
    @saminariaz9052 3 месяца назад +1

    One of the best videos on hplc👍

  • @MuhammadBilal-ln4ft
    @MuhammadBilal-ln4ft 2 месяца назад

    I was looking for this complete guide. Thanks for sharing

  • @educationpoint778
    @educationpoint778 Год назад

    really good informative video. great work. i am phd student from china

  • @amit4647
    @amit4647 3 года назад +8

    only 1L solvent 15 min sonication is not need its 1min per 1L, for filtering samples never use something to pull sample into syringe.. you should pour from top with plunger removed and filter attached. and never ever fill hplc vial past the 1.5ml mark. very nice instrument i wish i used that in school

  • @mahdiseif6242
    @mahdiseif6242 8 месяцев назад

    Thank you for this video. It was so informative🌹

  • @SayukeRecintoOtaku
    @SayukeRecintoOtaku 2 года назад

    Thank you for the video and I'd like to say that I loved to hear Big Bang's song "Monster" as a soundtrack!

  • @ramlakamal4076
    @ramlakamal4076 3 года назад +3

    Thank you very much for this video tutorial. It is really enlightening.

  • @From_the_city_of_Queen_Jhansi
    @From_the_city_of_Queen_Jhansi 9 месяцев назад

    Very well explanation ✌️

  • @mahammadajaruddin.s
    @mahammadajaruddin.s 2 года назад

    Thank you so much Myam.. Because of i have gained to the lot of information ...

  • @hellenlabol1102
    @hellenlabol1102 2 года назад +1

    Thanks for such nice educative illustrations

  • @owaisqadri1441
    @owaisqadri1441 2 месяца назад

    Very nice presentation

  • @bakhodirjonkakhkharov344
    @bakhodirjonkakhkharov344 Год назад +1

    Thank you very much! Great job!

  • @Unstopable27
    @Unstopable27 2 года назад +2

    Muy buen video! Hubiera faltado explicar cómo purgar el equipo, y cómo colocar y lavar la columna por si hay que correr más de un compuesto
    Gracias por la explicación! 😁

  • @dr.maqsoodahmad8572
    @dr.maqsoodahmad8572 Год назад

    Thanks for a comprehensive information.

  • @chemistryabcxyz2763
    @chemistryabcxyz2763 6 месяцев назад

    This is a very good video. Well done

  • @Aminulchem
    @Aminulchem Год назад +1

    Excellent one.

  • @alekudavid
    @alekudavid 2 года назад

    Very interesting HPLC analysis method.

  • @Claireshoon
    @Claireshoon 2 года назад +1

    Thank you so much sharing such a useful knowledge.

  • @tw9200
    @tw9200 2 года назад

    Thank you for give to all information. This is very useful information .

  • @luckyhub7632
    @luckyhub7632 3 года назад +1

    Thank you so much for give to all information. This is very useful information . 👍🙏

  • @obedsanchez5182
    @obedsanchez5182 6 месяцев назад

    Is big bang in your music 😍

  • @Urdulove1314
    @Urdulove1314 6 месяцев назад

    such a Clear guide

  • @Dr.AninditaDe-of1lk
    @Dr.AninditaDe-of1lk Год назад

    Really awasome. Thank u ❤

  • @adekunleajiboye1244
    @adekunleajiboye1244 11 месяцев назад

    Very concise. Thanks

  • @majdkittaneh1908
    @majdkittaneh1908 2 года назад

    Thank you for this video.. very helpful

  • @dushyantpatel6678
    @dushyantpatel6678 3 года назад +2

    great job this video is very useful , Thanks
    but my request to you please explain from desktop screen, So understand very clear.

    • @jassi6174
      @jassi6174 3 года назад +1

      Yes even I want to understand that software things. Plz make a detailed video on method development. Thank you so much for this video..v helpful 🙏👍

  • @mdasrafulislamkhan7260
    @mdasrafulislamkhan7260 Год назад

    Thanks u boss from the core ❤of my heart 💞❤️❤

  • @04deshmukhanas67
    @04deshmukhanas67 2 года назад +1

    Thank you so much
    Great job

  • @md.abdurrahmanrahad
    @md.abdurrahmanrahad 11 месяцев назад

    Thanks you so much,, Give me a good Advice.

  • @aliceyonemin241
    @aliceyonemin241 Год назад

    That was useful, thank you so much

  • @matchmedicines8209
    @matchmedicines8209 2 года назад

    it was a great information, thank you so much

  • @peaceandlove9131
    @peaceandlove9131 3 года назад

    Thank you so much for this great video

  • @kiyanazare5668
    @kiyanazare5668 Год назад

    That was awesome thank you

  • @nitikshamistry2040
    @nitikshamistry2040 3 года назад

    Thank you so much for this Great video. Thank you.........

  • @MuhammadNaveed-bw8ob
    @MuhammadNaveed-bw8ob 2 года назад

    What a great sir

  • @ahmedalkhdri9562
    @ahmedalkhdri9562 Год назад

    Thank you so much for this wonderful illustration video

  • @juanpena8642
    @juanpena8642 7 месяцев назад

    AWESOME VIDEO! Does anyone know what the results mean? particularly the first distribution it goes in the negative. What does this mean? Thank you!

  • @SonooDixit
    @SonooDixit Год назад

    V.nice

  • @antocharles4203
    @antocharles4203 2 года назад

    excellent

  • @木子玉-d6m
    @木子玉-d6m 3 года назад

    thanks for your useful video! It helps me a lot!

  • @rk-tube24
    @rk-tube24 2 года назад

    Thank you so much ❤️😀

  • @okechukwuernest8844
    @okechukwuernest8844 2 года назад +2

    Thanks a lot but I would love to see the appearance of those peaks in the chromatogram while running the analysis. Also the software is not clear enough for us to see.

  • @nancymohamed1656
    @nancymohamed1656 3 года назад

    great work

  • @BinteNavaira
    @BinteNavaira 3 месяца назад

    Use the solvent transfer into HPLC vial through syringe. This is solvent was filter and sonicated. It's true . But why color is change.

  • @vilasbirajdar6998
    @vilasbirajdar6998 2 года назад

    thank you so much🙂🙂🙂

  • @tareksaber2018
    @tareksaber2018 3 года назад

    thank you very very much , this was very helpful.

  • @landofmyth221
    @landofmyth221 2 года назад

    Nice guide

  • @azlinaak
    @azlinaak 11 месяцев назад

    Hi Nurul.How to save the data using excel/notepad. Because I need the raw data to plot in Origin software.

  • @aishayesha3458
    @aishayesha3458 3 года назад

    Ah niceee♥️

  • @TSoul-fp4vq
    @TSoul-fp4vq 2 года назад +1

    16:02 cmon good sir I'm tryin to learn here and you bustin out Bigbang Monster song I can't focus no more. Came in for HPLC, got out as VIP.

  • @BinteNavaira
    @BinteNavaira 3 месяца назад

    What is First solvent name used for filteration?

  • @villagenaturalcooking2935
    @villagenaturalcooking2935 2 года назад

    Thanks sir

  • @DhabitahJamal
    @DhabitahJamal 6 месяцев назад

    Hi, i want to ask how to set up gradient elution in this softwarr?

  • @ravidhyani1011
    @ravidhyani1011 3 года назад

    Thanks for this video

  • @kashifkumbher3602
    @kashifkumbher3602 2 года назад +1

    Which type of 4 solvents used for tubing?

  • @Redemption4U
    @Redemption4U 3 года назад +2

    Thanks for the great video, so informative, please do you do online courses?

  • @aysegocer3308
    @aysegocer3308 Год назад

    👏

  • @Carmelo12320
    @Carmelo12320 3 года назад

    thank you

  • @vipulkumar7035
    @vipulkumar7035 Месяц назад

    why filter the solvents which are already HPLC grade?

  • @seymacnar3610
    @seymacnar3610 Год назад

    👍🏻😍

  • @giantbird
    @giantbird 4 месяца назад

    thanks do you have tutor for 1290?

  • @eugenenyanmaomy8804
    @eugenenyanmaomy8804 3 года назад

    very helpful

  • @vlog19sm78
    @vlog19sm78 3 года назад

    thanks you so much

  • @bxmx4627
    @bxmx4627 Год назад

    К сожалению я не говорю на английском. Из иностранных языков только русский, но достаточно хорошо. Я из Узбекистана, преподаю органическую химию специалистам медикам в одном из учебных заведений. У меня грузом лежит новый оборудование HPLC компании агилент. рабочий стол и интерфейс на английском языке и я не могу загрузит данные на компьютер. Вы не можете показать на примере последовательность загрузки работы на компьютер. Заранее спасибо.

  • @nusratsubhan3705
    @nusratsubhan3705 3 года назад

    This is very nice and informative vedio, could you please open this to download the vedio which will help us more.

  • @LeNguyen-im8dm
    @LeNguyen-im8dm Год назад

    How long does it take to train someone to use this machine?

  • @BinteNavaira
    @BinteNavaira 3 месяца назад

    Deionized water also called distilled water it's rue

  • @jonsolkar
    @jonsolkar 3 года назад

    Thanks 👍👍

  • @rupalimahadik2424
    @rupalimahadik2424 6 месяцев назад +2

    You are not doing purging

  • @landofmyth221
    @landofmyth221 2 года назад +1

    Still, the computer part of the HPLC sooo fuckn confusing

  • @manjiris.shinde4302
    @manjiris.shinde4302 3 года назад

    The video is great but did not understood what all went on software. Wish the steps were more clearer

  • @LL_INHIBITION
    @LL_INHIBITION Год назад

    Can someone tell me with mobile phase is best for KHP

  • @frennezemoran6212
    @frennezemoran6212 2 года назад

    May I know the measurements of the chemicals

  • @franciemahap6580
    @franciemahap6580 3 года назад

    Does the fitration unit comes with the HPLC or do your order separately? Am thinking of ordering one.

  • @giselemaia_
    @giselemaia_ 3 года назад

    why the music?

  • @rddept.santej7571
    @rddept.santej7571 2 года назад

    05:50 don't forget to label 🤣

  • @kotireddyvarikuti6261
    @kotireddyvarikuti6261 3 года назад

    Sir empowere video kavli sir

  • @royalstage507
    @royalstage507 2 месяца назад

    SIR may i get your no..sir i want to know about voltametry...plzz sir make videos on voltametrey

  • @dineshdwivedi9026
    @dineshdwivedi9026 Месяц назад +1

    Contact me

  • @aliceyonemin241
    @aliceyonemin241 Год назад

    That was useful, thank you so much

  • @NishithSir
    @NishithSir 3 года назад

    Thank you so much