Thank you for the well edited footage and showing every step in running a sample with one method in the sequence. Our HPLC just came in this week! The maintenance technician said 100mcL was the max injection and did not mention a need to filter samples or solvents. We will follow these protocols as we get comfortable with the machine :)
only 1L solvent 15 min sonication is not need its 1min per 1L, for filtering samples never use something to pull sample into syringe.. you should pour from top with plunger removed and filter attached. and never ever fill hplc vial past the 1.5ml mark. very nice instrument i wish i used that in school
Muy buen video! Hubiera faltado explicar cómo purgar el equipo, y cómo colocar y lavar la columna por si hay que correr más de un compuesto Gracias por la explicación! 😁
Thanks a lot but I would love to see the appearance of those peaks in the chromatogram while running the analysis. Also the software is not clear enough for us to see.
К сожалению я не говорю на английском. Из иностранных языков только русский, но достаточно хорошо. Я из Узбекистана, преподаю органическую химию специалистам медикам в одном из учебных заведений. У меня грузом лежит новый оборудование HPLC компании агилент. рабочий стол и интерфейс на английском языке и я не могу загрузит данные на компьютер. Вы не можете показать на примере последовательность загрузки работы на компьютер. Заранее спасибо.
This has been a super clear stepwise guide. Just as if I am there running the test. Thank you.
Thank you for the well edited footage and showing every step in running a sample with one method in the sequence.
Our HPLC just came in this week! The maintenance technician said 100mcL was the max injection and did not mention a need to filter samples or solvents. We will follow these protocols as we get comfortable with the machine :)
mcl means?
@@rajasaravanan1967 microliter
@@aaronwhitt1636 thanks brother
One of the best videos on hplc👍
I was looking for this complete guide. Thanks for sharing
really good informative video. great work. i am phd student from china
only 1L solvent 15 min sonication is not need its 1min per 1L, for filtering samples never use something to pull sample into syringe.. you should pour from top with plunger removed and filter attached. and never ever fill hplc vial past the 1.5ml mark. very nice instrument i wish i used that in school
Thank you for this video. It was so informative🌹
Thank you for the video and I'd like to say that I loved to hear Big Bang's song "Monster" as a soundtrack!
Thank you very much for this video tutorial. It is really enlightening.
Very well explanation ✌️
Thank you so much Myam.. Because of i have gained to the lot of information ...
Thanks for such nice educative illustrations
Very nice presentation
Thank you very much! Great job!
Muy buen video! Hubiera faltado explicar cómo purgar el equipo, y cómo colocar y lavar la columna por si hay que correr más de un compuesto
Gracias por la explicación! 😁
Thanks for a comprehensive information.
This is a very good video. Well done
Excellent one.
Very interesting HPLC analysis method.
Thank you so much sharing such a useful knowledge.
Thank you for give to all information. This is very useful information .
Thank you so much for give to all information. This is very useful information . 👍🙏
Is big bang in your music 😍
such a Clear guide
Really awasome. Thank u ❤
Very concise. Thanks
Thank you for this video.. very helpful
great job this video is very useful , Thanks
but my request to you please explain from desktop screen, So understand very clear.
Yes even I want to understand that software things. Plz make a detailed video on method development. Thank you so much for this video..v helpful 🙏👍
Thanks u boss from the core ❤of my heart 💞❤️❤
Thank you so much
Great job
Thanks you so much,, Give me a good Advice.
That was useful, thank you so much
it was a great information, thank you so much
Thank you so much for this great video
That was awesome thank you
Thank you so much for this Great video. Thank you.........
What a great sir
Thank you so much for this wonderful illustration video
AWESOME VIDEO! Does anyone know what the results mean? particularly the first distribution it goes in the negative. What does this mean? Thank you!
V.nice
excellent
thanks for your useful video! It helps me a lot!
Thank you so much ❤️😀
Thanks a lot but I would love to see the appearance of those peaks in the chromatogram while running the analysis. Also the software is not clear enough for us to see.
great work
Use the solvent transfer into HPLC vial through syringe. This is solvent was filter and sonicated. It's true . But why color is change.
thank you so much🙂🙂🙂
thank you very very much , this was very helpful.
Nice guide
Hi Nurul.How to save the data using excel/notepad. Because I need the raw data to plot in Origin software.
Ah niceee♥️
16:02 cmon good sir I'm tryin to learn here and you bustin out Bigbang Monster song I can't focus no more. Came in for HPLC, got out as VIP.
What is First solvent name used for filteration?
Thanks sir
Hi, i want to ask how to set up gradient elution in this softwarr?
Thanks for this video
Which type of 4 solvents used for tubing?
Thanks for the great video, so informative, please do you do online courses?
👏
thank you
why filter the solvents which are already HPLC grade?
👍🏻😍
thanks do you have tutor for 1290?
very helpful
thanks you so much
К сожалению я не говорю на английском. Из иностранных языков только русский, но достаточно хорошо. Я из Узбекистана, преподаю органическую химию специалистам медикам в одном из учебных заведений. У меня грузом лежит новый оборудование HPLC компании агилент. рабочий стол и интерфейс на английском языке и я не могу загрузит данные на компьютер. Вы не можете показать на примере последовательность загрузки работы на компьютер. Заранее спасибо.
This is very nice and informative vedio, could you please open this to download the vedio which will help us more.
Snaptupe is there
How long does it take to train someone to use this machine?
Deionized water also called distilled water it's rue
Thanks 👍👍
You are not doing purging
Still, the computer part of the HPLC sooo fuckn confusing
The video is great but did not understood what all went on software. Wish the steps were more clearer
Can someone tell me with mobile phase is best for KHP
May I know the measurements of the chemicals
Does the fitration unit comes with the HPLC or do your order separately? Am thinking of ordering one.
why the music?
05:50 don't forget to label 🤣
Sir empowere video kavli sir
SIR may i get your no..sir i want to know about voltametry...plzz sir make videos on voltametrey
Contact me
That was useful, thank you so much
Thank you so much