Axion Labs & Training Institute
Axion Labs & Training Institute
  • Видео 85
  • Просмотров 227 166
How does mass spec work?
Watch the full webinar on Axion Lite - Free HPLC and GC training.
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Subscribe for more insights into chromatography techniques and applications.
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Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you c...
Просмотров: 276

Видео

What's unique about Axion courses?
Просмотров 3495 месяцев назад
See what an Axion course is like. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: axionlabs.com/ Facebook: axionlabs LinkedIn: www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or h...
Everything you need to know about HPLC flow rates
Просмотров 6565 месяцев назад
HPLC flow rates very depending on the column diameter. In this video we talk about columns ranging from 2.1 to 50 millimeter ID. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: axionlabs.com/ Facebook: axionlabs LinkedIn: www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResea...
When to use a gradient in HPLC?
Просмотров 9095 месяцев назад
How do you know when you should use an gradient elution instead of isocratic elution? In this exploration of gradients in chromatography, we unpack the pivotal question: when is a gradient necessary? The heart of the matter lies in the hydrophobic similarities of the molecules being analyzed. If they share similar hydrophobicities, a gradient might not be needed, as indicated by a scouting run ...
Dwell Volume vs Dead Volume
Просмотров 5615 месяцев назад
Dwell Volume vs Dead Volume
Monomeric vs Polymeric C18 Columns
Просмотров 2416 месяцев назад
In this video, we dive into the world of chromatography to understand the difference between monomeric and polymeric C18 columns. We break down the technical jargon to explain how these columns work and their significance in chromatographic separations. From the basics to practical applications, join us as we unravel the mysteries behind these essential tools in the lab. Subscribe for more insi...
Can I run an HPLC gradient from 0 to 100%?
Просмотров 3686 месяцев назад
For related videos watch: Can you run 100% water with a C18 column? ruclips.net/video/xwMYDUYPJR0/видео.html HPLC Problems: Very polar molecules. ruclips.net/video/HZV5B_p6OeM/видео.html
What is void volume, void time, t0 and solvent front?
Просмотров 6126 месяцев назад
What is void volume, void time, t0 and solvent front?
HPLC Tips: How to calculate void volume
Просмотров 1,4 тыс.7 месяцев назад
In this video we delve into the critical aspect of calculating void volume in High Performance Liquid Chromatography (HPLC). Understanding the void volume, or the space within the column not occupied by the stationary phase, is essential for precise chromatographic analysis. We start by introducing T0 measurement-how to determine the time at which solutes pass through the column without interac...
Can you run 100% water with a C18 column?
Просмотров 6737 месяцев назад
🔬 Mastering Your C18 Column: Essential Tips for Chromatographers 🔬 Welcome to our latest video where we dive into the nuances of using a C18 column in chromatography. Whether you're a seasoned chemist or just starting out, this video is packed with valuable insights and tips to help you get the most out of your C18 column. 👉 What's Inside: The Water Dilemma: Can you run your C18 column with 100...
HPLC problems with very polar molecules.
Просмотров 4438 месяцев назад
🔬 Welcome to the world of HPLC columns, specifically focusing on the 'Polar Embedded Phase' technology! 🔍 In this video, we unravel the intricacies of these highly efficient columns. If you're a scientist, researcher, or anyone interested in analytical chemistry, this video is for you! 🌊 What Are Polar Embedded Phases? 🌊 Discover the unique 'Polar Embedded Phase' columns in HPLC technology. We ...
Do I need to use an HPLC Guard Column?
Просмотров 6498 месяцев назад
In this insightful video we focus on the use of guard columns. We answer these questions: What's the deal with the guard column? Do I need one? Is it required? What's it for? 🔬 What You'll Learn: The fundamental purpose of a guard column in chromatography. How to decide if a guard column is necessary for your specific application. Tips for maintaining your analytical column and extending its li...
Best solvent to dissolve HPLC samples
Просмотров 7879 месяцев назад
🔬 Welcome to our latest educational video on chromatography! In this comprehensive guide, we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. Whether you're a seasoned chemist or a student just starting out, this video offers valuable insights for everyone. 🌟 What's Inside: Impact of Sample Solvent on Chromatography: We start by exploring how ...
How to test a LC column?
Просмотров 4989 месяцев назад
In this video, we dive deep into the best ways to test an LC column's performance to ensure optimal results. We'll explore different methods for testing columns, including using specific probes like dimethyl aniline and quinizarin to check for acidity and metal content. However, we emphasize a more practical approach: using your daily standard samples to evaluate column efficiency and peak shap...
How to clean a C18 column?
Просмотров 4,2 тыс.10 месяцев назад
🔬 In this video guide, we explore the necessary techniques for maintaining the efficiency of your C18 column in chromatography. Our expert tips help identify column issues and offer practical cleaning solutions. 🌐 Highlights of the Article: Identifying Column Issues: Learn how to detect and prevent permanent damages to your HPLC column. Dealing with Dirty or Clogged Frits: Discover the reasons ...
What's the difference between HPLC & UPLC?
Просмотров 2,4 тыс.10 месяцев назад
What's the difference between HPLC & UPLC?
HPLC Tubing & Fittings
Просмотров 1,5 тыс.11 месяцев назад
HPLC Tubing & Fittings
HPLC Sample Prep Basics
Просмотров 3,7 тыс.Год назад
HPLC Sample Prep Basics
Ghost Peaks in HPLC - 5 common sources
Просмотров 2,4 тыс.Год назад
Ghost Peaks in HPLC - 5 common sources
How long does it take for a column to equilibrate?
Просмотров 1,3 тыс.Год назад
How long does it take for a column to equilibrate?
Problems caused by dwell volume
Просмотров 1 тыс.Год назад
Problems caused by dwell volume
What is dwell volume?
Просмотров 2,3 тыс.Год назад
What is dwell volume?
Dr. Polite's Teaching Style
Просмотров 192Год назад
Dr. Polite's Teaching Style
HPLC - Negative Peaks and Baseline Drift
Просмотров 6 тыс.Год назад
HPLC - Negative Peaks and Baseline Drift
Meet Dr Polite
Просмотров 240Год назад
Meet Dr Polite
It doesn't matter which brand GC or HPLC you use.
Просмотров 522Год назад
It doesn't matter which brand GC or HPLC you use.
Hone Your Chromatography Skills at Our Chromatography Happy Place
Просмотров 277Год назад
Hone Your Chromatography Skills at Our Chromatography Happy Place
Tour of Axion Labs
Просмотров 325Год назад
Tour of Axion Labs
HPLC Troubleshooting: What is causing baseline noise?
Просмотров 13 тыс.Год назад
HPLC Troubleshooting: What is causing baseline noise?
# 1 Most Common Problem in HPLC: Air Bubble in the Pump
Просмотров 27 тыс.Год назад
# 1 Most Common Problem in HPLC: Air Bubble in the Pump

Комментарии

  • @imadkheldoun6379
    @imadkheldoun6379 День назад

    Amazing explanation thank you sir

    • @axionlabs
      @axionlabs 13 часов назад

      You are welcome

  • @elit_andrew
    @elit_andrew 26 дней назад

    How do I remove the bubbles in Agilent Infinity II 1260 series

  • @kalrbaum.
    @kalrbaum. Месяц назад

    Exactly what i was looking for, thanks!

  • @BlahBlah-gb6st
    @BlahBlah-gb6st 2 месяца назад

    What could be wrong if my inlet total flow falls when the oven temp is ramping up? I have changed the septum.

  • @srirampmna135
    @srirampmna135 2 месяца назад

    Sir how to calculate the delay time from detector to fraction collection tube .Could u pls share ur ideas ?

  • @LaLa-qf1qz
    @LaLa-qf1qz 2 месяца назад

    You are a great speaker. Very confident and helpful, thank you

  • @gooddaniel8603
    @gooddaniel8603 2 месяца назад

    How do I apply to this class ?

    • @axionlabs
      @axionlabs 4 дня назад

      Go to axionlabs.com/lc-gc-bootcamp/. This is the hands-on class. We also offer on-demand courses.

  • @Ccccccccccsssssssssss
    @Ccccccccccsssssssssss 2 месяца назад

    Thanks for the great video!

  • @lucasclementinomourao5677
    @lucasclementinomourao5677 3 месяца назад

    I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?

  • @srirampmna135
    @srirampmna135 3 месяца назад

    Sir, how does the molecule come.down when we place positive charge repeller .

  • @srirampmna135
    @srirampmna135 3 месяца назад

    Sir, could u pls explain about mass tuning in one video.

  • @bsadeghi1098
    @bsadeghi1098 3 месяца назад

    Thats very great,thanks for these useful points,could you please explain the thermostat and autosampler parts too?how can i understand the effeciency of these two parts

  • @mohamedislambenghaname133
    @mohamedislambenghaname133 3 месяца назад

    Is there any good method for erythropoietin in albumin serum ?

  • @denniswhv7546
    @denniswhv7546 4 месяца назад

    wow thanks!

  • @Will_be_back_by_2024
    @Will_be_back_by_2024 4 месяца назад

    I like you! You're very practical

    • @axionlabs
      @axionlabs 3 месяца назад

      I appreciate that!

  • @tayyabaraja433
    @tayyabaraja433 4 месяца назад

    Sir can u please tell me... Which type of standards used for the Hplc of The sample of TSB broth inoculated with bacterial strains for growth with crude oil?

  • @Sarahishamvasileva
    @Sarahishamvasileva 4 месяца назад

    How i can learn abt calibration, is it available for gcms

  • @wahmfarad2719
    @wahmfarad2719 4 месяца назад

    Dear sir you explained well..

  • @137bob3d
    @137bob3d 5 месяцев назад

    this Axion guy gets my vote for having fab' knowledge at the tip of his tongue

  • @dewittk9404
    @dewittk9404 5 месяцев назад

    I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove/prevent this?

  • @user-nm4yb7gl5u
    @user-nm4yb7gl5u 5 месяцев назад

    Great content! Thank u so much for sharing this!!

  • @Phasianidaes
    @Phasianidaes 6 месяцев назад

    Great explanation! This is critical when transferring methods into a lab with different brand and models

  • @heboottoobigforhegotdamnfeet
    @heboottoobigforhegotdamnfeet 6 месяцев назад

    How to deal with negative peaks on refractive index?

  • @MOHDSALEEM-gh7tf
    @MOHDSALEEM-gh7tf 6 месяцев назад

    Thank you sir for clearing my doubts 😊

  • @leewilliam3417
    @leewilliam3417 6 месяцев назад

    Thanks so much 😊

    • @axionlabs
      @axionlabs 3 месяца назад

      You're welcome 😊

  • @marcoromo9252
    @marcoromo9252 6 месяцев назад

    how does it work if using an internal standard and using their ratios?

  • @shreem3859
    @shreem3859 6 месяцев назад

    Very pocket friendly PI... fellows from this lab will be very lucky

  • @user-gc1ys5sh8v
    @user-gc1ys5sh8v 6 месяцев назад

    HI Lee indeed inspiring,

    • @axionlabs
      @axionlabs 3 месяца назад

      Glad you think so!

  • @srirampmna135
    @srirampmna135 6 месяцев назад

    Iam facing issue in gcms as it shows vial out of range.hiw can I fix it

  • @user-yz8lr6zv4u
    @user-yz8lr6zv4u 6 месяцев назад

    What HPLC machine is good enough to check Thc potency?

    • @MrSpicabooo
      @MrSpicabooo Месяц назад

      The best right now are waters arcs hplc amd waters acuity uplc

  • @srirampmna135
    @srirampmna135 6 месяцев назад

    Can u pls explain how to setup peak purity and threshold in spectra option in agilent

  • @srirampmna135
    @srirampmna135 6 месяцев назад

    I wish I could work under u and learn .Ur videos r really informative and interesting

  • @srirampmna135
    @srirampmna135 6 месяцев назад

    Couod u please explain how to set the peak purity and threshold in agilent

  • @srirampmna135
    @srirampmna135 6 месяцев назад

    I wish I could work under u and learn

  • @yerielhiciano7635
    @yerielhiciano7635 7 месяцев назад

    What about highly acidic or basic samples? Will that affect the C18 ligands of a column. I work with human serum and plasma that are treated with acid, precipitated and the supernate is injected so not the same as the mobile phase.

  • @srirampmna135
    @srirampmna135 7 месяцев назад

    Could u pls explain how to setup peak purity setup in spectra option in agilent

  • @user-vr6ol2hj3d
    @user-vr6ol2hj3d 7 месяцев назад

    wow how excellent video ! this video was very helpful for me, but i want to know amount of sample using calibration curve you described

  • @MrScorpioAngel
    @MrScorpioAngel 7 месяцев назад

    good cure to learn!

  • @srirampmna135
    @srirampmna135 7 месяцев назад

    Thank you soo much explaining and making such informatve video.Looking for more such videos .Can you please do a video on peak purity and independent column compartment port switching in agilent in detail

  • @rpsly1873
    @rpsly1873 7 месяцев назад

    Excellent explanation, awesome video

    • @axionlabs
      @axionlabs 3 месяца назад

      Glad you liked it!

  • @MemoreSPalay
    @MemoreSPalay 7 месяцев назад

    Thanks for this knowledge but I have a question Sir. Does pH also affect resolution and how?

  • @srirampmna135
    @srirampmna135 7 месяцев назад

    Can u explain how to check whether uv lamp.intensity has reduced or.not?

  • @srirampmna135
    @srirampmna135 7 месяцев назад

    I find this video and channel very useful and informative.Super and amazing

    • @axionlabs
      @axionlabs 3 месяца назад

      Thank you so much 🙂

  • @srirampmna135
    @srirampmna135 7 месяцев назад

    Amazing .where is ur lab ?

    • @axionlabs
      @axionlabs 3 месяца назад

      It's in Chicago. Check out our lab here axionlabs.com/lc-gc-bootcamp/

  • @kirranaslam6077
    @kirranaslam6077 8 месяцев назад

    when there is the need of new claibration table then a option of then this table would be deleted??

  • @nadeemiqbal4742
    @nadeemiqbal4742 8 месяцев назад

    Thank you sir.

  • @andyrieder3345
    @andyrieder3345 8 месяцев назад

    I am assuming Courts use the GC method right?

  • @muhamedhammoud9312
    @muhamedhammoud9312 8 месяцев назад

    love the videos, really helped me in my studies, great explanation, thanks!

  • @MrScorpioAngel
    @MrScorpioAngel 9 месяцев назад

    always best advices🙂

  • @MoodysZed
    @MoodysZed 10 месяцев назад

    Great video. How simple easy and concise!

    • @axionlabs
      @axionlabs 9 месяцев назад

      Glad it was helpful!