Polymerase Chain Reaction (PCR) Live demonstration. Practical process for PCR
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- Опубликовано: 22 авг 2024
- Hello and Welcome to everyone. Guys this is an attempt from our side to simply the concept of PCR and live demonstration so that students and researchers belonging to other streams could understand it practically. We have not highlighted the composition of reaction mixture because it varies according to condition of primer annealing, type of PCR and DNA samples. For queries related to PCR and reaction mixture, you contact us @ampliconsofbiotech and also can comment in the comment section.
Make sure to Subscribe to our channel and hit the like button if you liked our video, don't forget t share relevant ones.
This is a fantastic video for learning and getting knowledge..For the reson of COVID I am unable to learn practical techniques and handle of machines and apparatus..and in rural areas Universities there is no advance lab present..Thank you for this amazing demonstration..Plz sir and mam ..make many more technical demonstration of life science practical..There shall be I remain grateful..🙏
Thank you for the appreciation. Stay connected with us as we are trying our best to cover all the basic and advance practical techniques.
@@ampliconsofbiotech Thank you so much 🙏🙏
Awesome U explaining in fabulous way
you shouldn't have added the background music, it's very distracting.
Ok from next time I will not add
💯💯
After travelling through so many channels finally i got the right one..thanks for uploading such videob
👍we will upload more pcr related videos on our channel, make sure to subscribe for the updates 😊 thank you!
This is much needed and bringing best out of demonstration and will helps each student individually for instruments and handling. Thanks you Amplicons of biotech.😍
Most welcome @rajeshtamatta9068 we are happy to see that you are satisfied with our video and it helped you understand pcr with more clarity in easiest way. 😊
@@ampliconsofbiotech yes in most clearly and effective way again thanks
@@learnthefacts9068Lllll4rlll4rlllll
You're doing awesome things ❤
it is really helpful for many students (like me thanks a lot 😇
Thank you 👍
Thank you . Crystal clear demonstration.
Happy to help
appreciate your effort because I found first time like this video on youtube keep it up.
Thank you so much for appreciation
Very informative and useful. Kindly continue the series of video and upload as much as possible all biotech lab experiments
Sure I will
Thank you sir and mam for explaining each and every part ❤
Best ....best.... video on PCR.... thank
you very much ❤
I have some questions Regarding PCR
1) 1 Ul kya hai....
1) 5U aur 1U taq ki quantity ko kaise nikal kisi rxn main.
3) Kis company ka "Taq polymerase" use karna hai, aur uski kitni quantity milti hai aur price bhi mention kar dijiyega. Please 🙏😌🙏
Thank you, we are happy that you liked our video. Your questions will be answered asap.
very informative and highly knowledgeable topic.
Thank you meghraj Sir ❤️
Thank you Dr. Raj. Keep supporting 👍🏻
@@ampliconsofbiotech ❤️
Great information Sir and Mam👏
Very helpful video sir. Thankuu so much
Thank you sir for this important information of PCR 🙏🙏🙏
It's our pleasure @pandurang let us know what else we can help you with, we are open to suggestions for more topics in biotechnology experiments and concepts.
It's a best way for learning....tnx
Most welcome
Very informative.. Fine explanation.. Thank you!!
❤
U both of u did a great job ....it help me alot ... thank you...
@simra it's our pleasure to hear that our video has helped you understanding PCR. Stay connected to the channel for more such videos, and let us know if we can help you and others in any topic related to Molecular biology, biotechnology, etc
Insightful.. 🤩
Thank you 😊
Great way of explaining
Loved it, thank you so so much for making this video🎉 .
Just great but, kindly also cover the gel doc system for the visualization of DNA. Thank u.
Yes for sure.
Bro good informative video
Amazing video tysm.. 🔥🔥💯💯
👍
Very helpful..
Thank you sir
very good explanation ✔
Thank you
Very Helpful! 😊👍
Thank you
love from Pakistan !❤
Just wow explanation
So much useful 💯
Informative💕
👍🏻🙂
Great efforts😊
Thank you
Thank you so much for the video. 🧬 sir can please show the result part through gel electrophoresis after a pcr.
We have already uploaded a gel electrophoresis video in general context, for these results we will soon upload 96 wells gel assembly video. To stay notified press the bell icon and make sure to subscribe. Thank you
Fantastic video
Thank you so much
@@ampliconsofbiotech thnk u sir
Fantasticks 👍🏻
Thank you
Thank you.very informative.
🙏
thanks it was very helpful
How to design primer... Can you make a video on it
Very good helpful 🙏
Thank you
In Pcr primer bind to target region of dna and with Polymerase enzyme further step start
my question is .
in template dna some portion where primer not bind when reaction start.
what about that portion where primer not bind .how this portion multiply
Very nice explains brother 😁😊
Thank you
Excellent video Sir, rise and shine
Thank you 😊
Great❤
Please make video on real time pcr , cDNA ,cDNA dilution, optimisation and then qpcr. Plzzz it is a request
Yes we will make a video and upload it soon
Thanks a lot.
Nice
Great❤❤❤❤
Sir iski quantity kaise decide karenge kaunsa component kitne quantity mein add karna hai ????
Good job bro
Thank you
Sir i have some queries. I made a gel of 1.8 % and my DNA ran faster then the ladder!!! I used primer of 218 bp and ladder of 50kb. My DNA bands crossed the 50kb band 😓😓😓.. (O.D was 1.6) annealing temp 58
If you are loading only DNA then go with 0.8% gel, and if it's PCR product try loading 100bp ladder because your product size is 218bp, in 2% or 3.5% gel (volt 90-120v)
@@ampliconsofbiotech and for how much time??
@@ampliconsofbiotech sir , I’m talking about PCR product. I even dont know how to make a reaction mixture calculation of DNA ( like how much DNA should I take from my extracted total DNA ) I just used one paper and followed it might be that’s y I failed in same. I need to contact u. Plz help
After loading samples you can check for amplification one hour later under uv light torch, on 3.5% gel the product of 218 bp will take 2 hours to separate
You can contact us at 9834128616 then according to your DNA, primer and reaction we can finalize a protocol
Good demonstration😊
Thanks
Thank you
👍
Thank u both
Sir please tell us career options after Btech Biotechnology,or after qualifying GATE..
What are the job opportunities after Btech Biotechnology... please sir
Thanks
.
Hello sir inorder to join in laboratory (like you ..) which degree we should complete bsc biotechnology or btech biotechnology ..
You can go with any of these but further you need to do atleast a master's degree after graduation
Thanku sir can u pls explain plate setup or template for conventional pcr
Yes we will try to make a separate video on conventional PCR
Yes please make one kn rtpcr as well
Yes we will prepare another video
Fantastic video sir. I have a doubt, I am using applied biosystems gradient PCR machine. When the cycle is completed it shows time remaining as 00. 0 hours at that time can I press the pause/ stop button?.
Yes, once the program is ended and temperature goes to 4 degrees you can click on stop/ pause button
@@ampliconsofbiotech Thanks for the reply.
Needful information for me
👍
Thank you bro❤️
👍
Any bubbles formation in final sample and we insert into PCR machine then variation in results
Bubbles don't create any problem, just a 30 second spin to the vials will sort everything
Kya RT PCR result ko -20 degree par result ko store kar skte h?
Yes we can store it.
Thank you guy's :)
👍
Thank you so much!
👍
👍🏻👍🏻
Continue other video
Yes sure
Q.1what is nucleic acid prob technology?
Q.2what is nucleic acid topology?
Probe- the use of DNA or RNA probes to detect particular gene sequences.
Topology- how the two complementary single strands are intertwined in Watson Crick model
Thankyoy so much sir 😊
👍
thank you
👍
Sir I want to know the method about origin determination
Please elaborate
Any video in English version please
Knowledge ache hai but video editing thore casual hai
Why did we change the temperature to 56degreescentigrade .
Dna extraction
High tem.m denature..
To get to the next step of annealing we keep temp 55-60 depending on the primers used.
Dear real time or thermal cycler Mae Kia faraq Hy ??
Kia thermal cycler Mae channel nhi hotae kiaa ??
Thermal cycler is just name for PCR
Also make video on gel electrophoresis
Already on channel, please visit videos section to watch
Sir can you make a vedio of reporting a hiv pcr
We work on plants
Sir intrest of DNA kon se step Mai add karinge
If you are adding individual components, you can add dna at starting step or the last step.
I am from pakistan.
Main ny app ko subscribe karleya.
Thank you 👍
Plz suggest how to make primer.
You how to design primer, or you can use available ones from different databases
Can we spin in centrifuge machine
Yes
👍
Thank you
I have a real time pcr pioneer excicyler 96 ,how can I use it
. I don't know ،
Just try following the manual. All machines are near about same, you sure will find out how to operate it.
Can heparin be used for pcr qualitative?
Or what will happen with result?
Hairpin PCR can be a useful tool for qualitative analysis of RNA molecules, but careful optimization and validation of the assay are necessary to ensure reliable results.
@@ampliconsofbiotech so does that mean results may be reliable ?
Bhai pcr thermal cycler mn eppendorf tube ka temp kitna hota hy.
Tube ka koe temperature nahi hota hai, machine ka temperature jyata hai 110 degrees tak jo tube ki capacity me hota hai isliye tube melt nahi hoti. Sometimes if low quality plastic wares are used then tubes might melt in pcr
Ok thank you bhai v.good video
What is 30 sec step between denaturation and annealing
There are overall 5 steps, Initial denaturation for 5 min then the cycle starts with (denaturation - annealing - extension) last final extension for 10 min
rt pcr pe vdo banaiye practical
Ok
Sir ham kese pta krte hain ki hame konse part of dna ki hi copies banani hai
By using region/trait specific markers, organisms genomes are available on data bases. Bioinformatics tools ki help se marker design karte hai aur fir usi region k sath wo marker/primer attach hokar amplify karta hai region ko.
@@ampliconsofbiotech sir jab dna ke specific part ki bahut sari copies ban jati Hain to unke sath me wo extra dna bhi to machine me rhta hoga fir ham only specific part ko kese separate krte hain
Extract kasy Kiya DNA?
ruclips.net/video/ZMpDJ_ztKZ8/видео.html
Watch this video to know how we isolated DNA?
Sir how to identify microorganism using sequencing?
Metagenomics is the technique for identification, it would be more helpful to answer if you could be more specific about the question.
@@ampliconsofbiotech identification of using Sanger sequencing.
Sanger sequencing of internal and external microbial cultures.
See the principle is when a pure PCR product of the 16S gene is obtained, sequenced, and aligned against bacterial/microbial DNA data base, then the microorganism can be identified. Now what exactly are the steps involved and how it's practically done is little bit complicated to explain in the comments
@@ampliconsofbiotech OK . Thanks a lot
Please can you be speaking more of english..🙏
My latest videos are completely in English, I do it selectively according to type of video.
😂 thanks 😊
👍
Bhaiya apni salary bato😊
94...96 do time Kyo lete h.... Means sequence me
Samples pe depend karta hai jaise kisi me standard is 95 but can vary from samples, kisi ka denaturation 94 pe hota hai to kisi ka 95
@@ampliconsofbiotech Lekin.... Aapne dono set kiya h.... Thoda bta dijiye..... Waise bahut bahut Dhanyawad...... Aapne reply kiya 🙏
Ok I got your question. Their are two steps for the reaction, first is denaturation at 95 or 94 for 4 to 5 minutes then comes the cycle of pcr (denaturation, annealing, extension) and at last final extension
@@ampliconsofbiotech thank you sir🙏
sir, it is different from rtpcr?
Yes
Last me infinite Kyo set krte h
Actually it's not literally kept or set infinite, like other steps it does not have any standard time for example it can be kept for hours minutes or long term storage if you can afford that much electricity to store samples at 4 degrees in pcr but no does that
@@ampliconsofbiotech thank you sir
Don't use music with study videos it's distracting
Ok, will keep the next without music
Not only you have the Indian accent that it is annoying by itself that you have to deal with the background music
Ok, sorry for the inconvenience caused by background music and Indian accent. I will try to improve next time
l
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