Enzymes (Part 3 of 5) - Lineweaver Burk Plot - Double Reciprocal Plot

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  • Опубликовано: 2 фев 2025

Комментарии • 124

  • @sogazelle
    @sogazelle 10 лет назад +103

    Mahfoud, God Bless you...I just failed my biochem first exam because I didn't grasp the concepts from the way the teacher explains them. And we started the enzyme chapter last week and I had a headache throughout the whole 2 hour class even after reading the book. But I just spent the most beautiful hour listening to your 5 videos on enzymes and I cried from the joy of how amazingly and simply you explained the concepts and I just understood the whole chapter. Thank you so much! You are blessed with the mind of a real teacher. I hope that because of you, I will pass my biochem class. Thank you.

    • @MoofUniversity
      @MoofUniversity  10 лет назад +23

      sogazelle Wow! I'm going through a bunch of comments saying "you're welcome," and I come across these incredibly kind words from you! THANK YOU for the kind words! I'm very happy to have helped you understand biochemistry! :]

  • @randomdude4628
    @randomdude4628 7 лет назад +1

    Thanks to you, cramming is no longer what it used to be. This is like a TV show, only more engaging.

    • @MoofUniversity
      @MoofUniversity  7 лет назад

      More engaging than a TV show? Really? What sort of TV shows are you watching, dude? Haha! There are some pretty great shows out there.

    • @randomdude4628
      @randomdude4628 7 лет назад

      Haha! That was meant with the utmost sincerity. With TV shows I find myself playing around on my phone within the first 10 minutes, but your video lessons are very interesting and genuinely make me want to watch more. Thank you for the great videos! :)

    • @MoofUniversity
      @MoofUniversity  7 лет назад

      Lol. Well, I'm glad you find my content that engaging and interesting. Thanks so much for watching and for really enjoying them.
      In case you're interested in taking TV show suggestions, here are some that I'm into at the moment and/or have enjoyed in the more recent past: Master of None, Narcos, Game of Thrones, Better Call Saul, Sherlock, and Peaky Blinders. If none of those float your boat, please feel free to continue watching my videos. Haha!

  • @DowaKaday
    @DowaKaday 10 лет назад +4

    all your videos give me an understanding of biochemistry in just a week when i cant understand 3 months of lectures from my lecturer. Bless you my good man! :)

    • @MoofUniversity
      @MoofUniversity  10 лет назад +2

      DowaKaday Lol. Wow! I'm happy to have helped, dude!

    • @MoofUniversity
      @MoofUniversity  10 лет назад +2

      DowaKaday ....and thanks for the blessings! :]

  • @001button
    @001button 10 лет назад +37

    saving my life one lecture at a time :)

  • @subsixtynk95
    @subsixtynk95 8 лет назад +6

    This man is a LEGEND! Ohhhh my GOD, the clarity is unreal!! Thank you so much!

  • @marcelkhoury6768
    @marcelkhoury6768 10 лет назад +6

    This is the best thing i have ever found on the internet and i am eternally grateful Mahfouf. Thank you.

    • @MoofUniversity
      @MoofUniversity  10 лет назад +1

      Mark Khoury Hahaha! What a compliment! Thanks, dude!

  • @ngrubs55
    @ngrubs55 8 лет назад

    Holy crap, it's all so clear to me now. I swear you have saved my blood pressure. I feel like I owe you for how relieved you have made me.

  • @amberlange9082
    @amberlange9082 8 лет назад +11

    You're seriously getting me though biochemistry, thank you so much!

  • @bhupendramahawar9010
    @bhupendramahawar9010 8 лет назад

    I think some people are trying to get the clinical explanation for this and not the mathematical one. I came here to watch the clinical part too.... but this is probably one of the best derivation i have seen and i wish i had watched this vid when i was learning this stuff for the first time! Great explanation. Thank you so much !

  • @matthew_bang
    @matthew_bang 6 лет назад +1

    i have a midterm coming up in less than a week and you are helping me so much

  • @MarzNet256
    @MarzNet256 8 лет назад

    I learned all this in cell biology about 30 years ago. You do a very good job explaining it.

  • @ayesha2014
    @ayesha2014 3 года назад +1

    I have an exam in 2.5 hours. Watching this because i just couldn't understand the x-intercept thingy. THANK YOU !!!!

    • @MoofUniversity
      @MoofUniversity  3 года назад +1

      You're very welcome! I hope you did well on the exam!

    • @ayesha2014
      @ayesha2014 3 года назад

      @@MoofUniversity I did !!! Thanks once again :)

    • @MoofUniversity
      @MoofUniversity  3 года назад

      @@ayesha2014 Awesome! You're very welcome!

  • @hb6839
    @hb6839 6 лет назад +1

    I thank heavens for the day I found your videos as they'll help me become a good doctor soon, god bless helpful people like you.

    • @MoofUniversity
      @MoofUniversity  6 лет назад +1

      RIght on. Thank you, and good luck, Doc!

  • @Silvergladegirl
    @Silvergladegirl 10 лет назад +1

    Thank you SO MUCH for this video and the others on biochemistry related stuff. YOU sir, are the reason I will pass biochemistry. You're my hero, seriously.

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      Wow! Thanks for the kind words, and you're very welcome! :]

  • @ayesha2014
    @ayesha2014 3 года назад +1

    Thank you for making the math in biochem easier :)

  • @urockit2011
    @urockit2011 8 лет назад +1

    A much better explanation than Khan Academy's! Thank you!

  • @Evrce
    @Evrce 10 лет назад +1

    Dang! i just graduated BCH from UCR and some friends asks me for help. Wish i found you sooner because you're very clear and helpful.

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      ***** Dang! If they need help, you can definitely have them contact me via email. :]

  • @chelseajenkins7325
    @chelseajenkins7325 7 лет назад +1

    You seriously saved my life!!!!!!

  • @alphamsesay333
    @alphamsesay333 3 года назад

    This video is all I needed for my presentation. Thanks and God bless you

  • @sharshal465
    @sharshal465 6 лет назад

    Awesome video, Appreciate the way you take your time to properly explain what is going on here.

  • @erkanbatti
    @erkanbatti 8 лет назад

    you have no idea how much you are helping me. thank you so much.

  • @jonqn07
    @jonqn07 8 лет назад +1

    thank you Moof University. You helped me understand this! I have no clue looking at the book here. Thanks for the little Vmax etc..explanation too.

  • @yomayranegron-torres2428
    @yomayranegron-torres2428 8 лет назад

    Amazing explanations!!.. You are helping me to excel this part of my USMLE Step 1 !!!

  • @camerongj1
    @camerongj1 9 лет назад

    This was a fantastic explanation of the L-B plot! Thank you very much.

  • @Rawmon94
    @Rawmon94 10 лет назад +1

    AWESOME! thanks! gonna ace that biochem test!

  • @FortisAquila14
    @FortisAquila14 10 лет назад +2

    God bless u and this day when i found you!

  • @jo-jr2dv
    @jo-jr2dv Год назад

    youre very good at explaining things

  • @MsMopanda
    @MsMopanda 10 лет назад +1

    Your wonderful. You should be a professor . Your calm, and adorable. I love you side comments. Thank you for saving my grade

    • @MoofUniversity
      @MoofUniversity  9 лет назад

      MsMopanda Lol. Thank you so much! You're far too kind!

  • @LetUsBeLazy
    @LetUsBeLazy 8 лет назад +1

    This video helped so much! Thank you!

  • @NoorKhan-tb9dl
    @NoorKhan-tb9dl 5 лет назад +1

    Dude you are the FREAKING BEST!!

  • @edgarmalesa2367
    @edgarmalesa2367 9 лет назад +1

    thanks man.. you the best ... like the way you explain things.. make them seem easy ... thanks again

  • @naamaxo
    @naamaxo 9 лет назад +2

    Hi thank you so much fre this! i just have one question. How is it possible to get a negative 1/[S] value in the first place? Why is the x-intercept in the 2nd quadrant to begin with? Thanks!

  • @dalaeloubeidi6254
    @dalaeloubeidi6254 9 лет назад

    Great job! I think you did a really good job of explaining all these concepts!

  • @Gera1988mtl
    @Gera1988mtl 7 лет назад

    Excellent presentation and explication, thanks man

  • @m.t1446
    @m.t1446 5 лет назад

    The last 5 hours of my life in 7 minutes. Not all heroes wear capes

  • @sphamguyo6551
    @sphamguyo6551 8 лет назад +1

    thank you

  • @ambrosemadongonda7630
    @ambrosemadongonda7630 2 года назад

    Thank you you have made it easier

  • @StanleyWilhelm
    @StanleyWilhelm 8 лет назад

    thanks for making this series

  • @treesageorge3258
    @treesageorge3258 8 лет назад +1

    well explained sir!! thanks :) but how did u derive "x intercept"?? (dint understnd that part)

  • @1991AngelAyala
    @1991AngelAyala 8 лет назад

    this video is magic

  • @maria11135
    @maria11135 9 лет назад +1

    you are legendary!!!

  • @emiliabri1781
    @emiliabri1781 5 лет назад +1

    Thank you!

  • @YoungBuddhaEzuk
    @YoungBuddhaEzuk 5 лет назад

    DUDE yes, thx so much 🤘🏽🤘🏽🙏🏽🙏🏽🔥🔥(Biochemistry)

  • @thailizvazquez6830
    @thailizvazquez6830 6 лет назад

    Thank you so much!!! Very helpful video! I could easily understand the graphs! :)

  • @amalabdallah2885
    @amalabdallah2885 10 лет назад +1

    I'm so grateful for this video.
    thank you soo much!!!!

  • @shazwannaim8543
    @shazwannaim8543 8 лет назад

    Really helped me to understand...thanks!!!!

  • @travisscott5779
    @travisscott5779 7 лет назад

    Thanks from Saudi Arabia i love u man u made easy for me may allah bless u

  • @sabrinarashidi3226
    @sabrinarashidi3226 Год назад

    This was so helpful but i have question for lineweaver burk plot does it have to be that straight line because my enzyme esterase graph it has curve ?

    • @MuthuThala-ih2wu
      @MuthuThala-ih2wu 9 месяцев назад

      Lineweaver developed this plot along with mathematicians to reduce the complexity for biology
      Students.
      In order to calculate for graphs with curves it needs calculus
      But for straight line graphs it's easy to calculate graph parameters..
      So all lineweaver plots will have straight line function
      U might have seen michealis menten graph for esterase...

  • @allaalbonijim2748
    @allaalbonijim2748 8 лет назад +1

    THIS IS AMAZING!!!!

  • @violinsheetmusicblog
    @violinsheetmusicblog 8 лет назад +2

    How is -1/Km derived?

  • @karinaaguilar4941
    @karinaaguilar4941 2 года назад

    Was here in 2018 when I took undergraduate biochemistry, from your videos I made an A in the class! Now here in 2022 for review because I have started a Biochemistry PhD and we are taking the class as our 1st year curriculum. Thank you!!!!!

    • @MoofUniversity
      @MoofUniversity  2 года назад

      That's so awesome! Congratulations! It's so awesome to be part of your journey! :]

  • @DanNguyen-hu6pt
    @DanNguyen-hu6pt 10 лет назад

    Thank you so much Mahfoud. This video is extremely helpful :)

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      Danny Nguyen You're very welcome! I'm glad you found it helpful! :]

  • @aishahhearts8294
    @aishahhearts8294 7 лет назад

    Thank you all I say is thanks seriously with all my heart❤️🌹🌹🌹 please keep it up your great videos

    • @MoofUniversity
      @MoofUniversity  7 лет назад

      You're very welcome! Thanks for watching! :]

  • @rareflower88
    @rareflower88 9 лет назад +1

    Thank you - really helpful!

  • @abigailnizahnambeye6959
    @abigailnizahnambeye6959 4 года назад

    Wow...thank you very much

  • @magic0chicklets393
    @magic0chicklets393 11 лет назад

    this helped me a lot!

  • @sanaabedouane1910
    @sanaabedouane1910 10 лет назад

    thank you so much it's very helpful that's helped me to see the concept of the enzymes :D

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      sanaa bedouane Sweet! I'm happy to have helped! :]

  • @fortecimo3547
    @fortecimo3547 9 лет назад

    gotta love this..thanks a lot

  • @1Shoda
    @1Shoda 10 лет назад

    Why didn't I look up up for u earlier??? Now I am stalking all ur kinetics video as I study for final!!!!

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      sneha hoda I don't know, dude. You should tell your study buddies about me and my channel, though! Lol. I hope you did well on that final.

  • @mustachegurl1714
    @mustachegurl1714 7 лет назад

    if the km is always positive. How can I plot it?

  • @drnazmy8128
    @drnazmy8128 7 лет назад +1

    thank you you saved my grades i will not fail in the course i guess

  • @merzouqhind4373
    @merzouqhind4373 4 года назад

    thank you so much

  • @raissacanales6103
    @raissacanales6103 8 лет назад

    what do you mean they flip over and make it easier by turning it into a line? i dont get that.

  • @udari111
    @udari111 11 лет назад

    loved it! thanks!

  • @RomajaneAbrenio
    @RomajaneAbrenio 8 лет назад

    Thank you again.

  • @georgelagalle2298
    @georgelagalle2298 6 лет назад

    Thank you so much, you made that so easy lol :)

  • @AlyssaCuriel
    @AlyssaCuriel 9 лет назад

    Thanks.

  • @kaileyrobertson8018
    @kaileyrobertson8018 9 лет назад

    Thank you so much!

  • @sarajs1394
    @sarajs1394 7 лет назад

    I wish we have only one of you at our uni!

  • @LordHancelotThe69th
    @LordHancelotThe69th 4 года назад

    THANKS!!!!

  • @arinsadeghi3155
    @arinsadeghi3155 8 лет назад

    Great series of videos, just as a friendly reminder that the "actual meaning of Km depends on specific aspects of reaction mechanism such as the number and relative rates of the individual steps. " Source: Lehninger Principal of Biochemistry 5th ed. page198.

  • @chetan_358
    @chetan_358 5 лет назад

    How to derive derive X = Y(Sf -D×Ks/Umax-P)

  • @rachellediab7751
    @rachellediab7751 8 лет назад

    THANK YOU

  • @MOalharbi19
    @MOalharbi19 11 лет назад

    thanks alot very usfel

  • @carmenbooth6350
    @carmenbooth6350 9 лет назад +1

    you are awesome!!!!!

  • @barakreshef5096
    @barakreshef5096 10 лет назад

    your vids are great, but this particular - does not load...:(
    could u please upload it again?

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      I'm not sure what the problem is, but the video is loading just fine on my end. I'm sorry, but I have no idea why it's not working for you. :/ Maybe try loading it again? Or using a different browser? I dunno.

    • @barakreshef5096
      @barakreshef5096 10 лет назад +1

      Ok - prbolem solved. just grabbed the time pointer to 0:02 so it started...great vids, again.

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      Barak Reshef Sweet! I'm happy you got it to work. Thanks, dude!

  • @agdoombagdoom8540
    @agdoombagdoom8540 4 года назад

    could not understand why km was positive?

  • @funsizedstoichiometry8572
    @funsizedstoichiometry8572 10 лет назад

    thank you :-)

  • @mimik9948
    @mimik9948 7 лет назад

    THANK YOUUUY

  • @darl2588
    @darl2588 10 лет назад

    I love you

    • @MoofUniversity
      @MoofUniversity  10 лет назад

      Da Rina Lol. Right back at ya! Thanks for the support!

  • @ifrahmalik1031
    @ifrahmalik1031 7 лет назад

    Thank you
    I understood everything you said but could barely see what you wrote...I make notes so sir please try to write in a more defined manner ☺

  • @andreachiarini4685
    @andreachiarini4685 4 года назад

    Incredibly unintuitive

  • @anupriyakp4073
    @anupriyakp4073 6 лет назад

    thank you