Retrovirus mediated Gene transfer | RMGT | procedure | Bio science
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- Опубликовано: 7 ноя 2020
- Retrovirus mediated Gene transfer | RMGT | procedure | Bio science
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• Retrovirus mediated Ge...
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• Plant biotechnology
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• Immunology
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ZOOLOGY :-
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This is indeed helpful! 👍🏻
Thanks a lot sir ❤ tomorrow is my exam and now i think confident enough to answer these question😊
Such a simple and detailed explanation loved it
The part that I find weird is how inserting host bacterial cells into embryo will initiate expression of desired gene in the progeny. Because the embryo already has it's own genome. If you can please explain...
👌👌👌👌👌👌 annaya..we want more videos for CSIR NET topics please annaya
Thank you so much sir for such a helpful information ❤❤
How host cell inoculate into bacterial cell culture in step 5?
Thank u mam luv from Jammu and Kashmir..
Simple and easy 💓
You are awesome 👍
Thank youu so much sir your videos are very helpfulll 😊
My pleasure
Thank you .. this video help me a lot .. really thank you🤗🤗
My pleasure
well explained
How would you join the sequence of RNA with the help of DNA ligases 🤔
Thank u sir ...well explained
My pleasure
Rat nhi mice....thanks for the explanation 🙇
Thanks bhai ❤️
Sir please make a video on axis formation in zebra fish.
Hybridoma technology and in vitro in vivo fertilization kuda explain cheyara sir
Worthy afford !!!✌️.......
Bravo
awesome explaination
Thanks
Thanks a lot sir
🙂🙂
Ulmately explaining tq
My pleasure
Superb ga cheparu sir
Thanks
@@bioscience6853 sir set syllabus topics explain cheyandii sir
Thankyou so much sir ♥️
My pleasure
Thank you sir 😍
My pleasure
thanku nice explanation
My pleasure
DNA of virus having desired gene will also have their own gene which might be lethal for host(embryo) .. these lethal gene will not have undesire affect on host(embryo)????
Thanking you broo🙏🙏
My pleasure
Well explained
🙂🙂
Thank uhh...
My pleasure
Sir notes v banake dena
Bohot kssstttt hua Cc on karke notes banate
Could you please update of iron absorption, transport, and storage.
As soon as possible. Help me for the exam.
Easy👍👍👍👍👍🙏🙏🙏🙏
Retroviral vector method ana RMGT method ana same ana annayya
is the restrictions enzymes also cuts the RNA? because dna and rna nucleotides are different and how they cut RNA also?//
thanks
Did u introduce host cells (bacterial culture) into the embryo?
Yes
Upload vedio on hybridization technique :probes (radioactive and non radioactive ), detection plzzz bro... upload soon
Sure dont worry!! ☺
@@bioscience6853 okay bro upload as soon as possible and next vedio indhadhan irukum nu namburen bro❣️👍
Can u provide notes?
Good effort....plz can u tell me drawbacks of this technique.?
Target gene is a dna fragment and the retrovirus genetic material is rna based. how can we intergrate a dna fragment into an rna genome?
Either the retrovirus has the reverse transcriptase enzyme or we provide it to convert the rna into dsDNA, then the integration process follows.
By the help of intergrase enzyme as shown in the video
It is not replicating in RNA genome it is replicating in cytoplasm of host cell which has dna as genetic material
Dude u just asked such a good question that even I forgot to use common sense 😂. The answer is very silly.
When the restriction enzyme cuts the desired dna which is double stranded. It cuts only single strand. So technically u have single strand which is then ligated into viral RNA of retrovirus
can we use this tech. in plants also?
No..
@@kalaiselvir9389 ok thank you
plz must reply?
Hi man! i contacted u on whatsapp yesterday, i hope that you see messages ! important stuff! answer me asap
Thank you so much sir🙏
My pleasure
Thank you sir
My pleasure
Well explained
Thank you