DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques
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- Опубликовано: 1 апр 2020
- #genetransfer
DNA microinjection | Embryonic stem cell mediated gene transfer | Gene transfer techniques
DNA microinjection is the dominating technique leading to random integration of a transgene via the introduction of DNA into the pronucleus of a developing zygote. Following fertilization of a mouse egg, the male and female pronuclei remain separated for a few hours before they fuse to make the zygotic nucleus, thus allowing for microinjection of the desired genes into the larger male pronucleus. Eggs that can survive the injections are transferred into the oviducts of a foster mother i.e., pseudopregnant female mice for the generation of Founder mouse, from which permanent transgenic lines can be established. The presence of transgenes in the offsprings can be identified by PCR analysis or Southern blot hybridization.
For embryonic stem cell mediated gene transfer, skip this video directly to 5.45
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Electroporation
Gene transfer techniques
Dna
Rna
Transcription
Translation
Proteins
Animal biotechnology
Animal transgenesis
Alampally phanindra
Phanindra gupta
molecular biology
dna microinjection method
microinjection method of gene transfer in plants
dna microinjection transgenic animals
microinjection gene transfer method
embryonic stem cell mediated gene transfer
microinjection gene transfer method animation
Beautifully explained..!
Thanks hope your channel grow well ..
My pleasure✨
TQ soo much for your clear explanation ❤ sir
Amazing explanation
My seminar topic is "gene transfer to animal cells" I was so confused about methods... But after watching this... I feel relaxed lil bit🙂 thank you sir 🙏 I hope I can...
Thank you
Thank you ☺️
Very nice and simplest explanation 👍👍
Thanks
Useful vedio. That helps to understan the topic clearly
My pleasure
Thank u sir I watch the full video that very clearly I appreciate u sir
Thank you
Super explanation sir. Thanq.
My pleasure
Thank u sir good lecture
My pleasure✨
sir please kindly makes the video about mitochondria and chloroplast gene transformer
sir plz help me ...about retroviral transfection plz send me link of that vedio of urs
U are awesome bro. Thnks
☺☺
Thank you so much
My pleasure
WHICH FACTOR RESPONSIBLE FOR THE NEXT GENERATION WILL TRANSFER INTO GENE TARGETED MICE OR NORMAL MICE.
Thank you brother you cleared all my doubts and now i am ready to take the seminar 🥰🥰🥰 keep posting more videos ✌️✌️✌️
Sure
Fan from Kashmir
Sir aap video rcording kis chij se karate hai bataye please
Sir can we introduce more than one target gene in the ES cells
Thank you sir
❤☺❤
I'm a little bit confused. In embryonic stem cell mediated gene transfer method, if the the target genes are incorporated into the ESCs and then added to blastocyst and finally when it is implanted into the host, why do the host has to undergo fertilization? Because after fertilization, the blastocyst will be formed.
Thnku so much
My pleasure
Thanks yrr
Sir where is retroviral gene transfer method topic?
When foreign DNA is transferred to the host cell, how does the foreign DNA bind to the nuclear DNA of the host cell?
Nice
Thanks
Sir explain in hind..and also explain other methods of transformation
Sirr which vector is used for microinjection plzzz reply
In microinjection technique, can we use sperm cell as a host.??After which we fertilize egg cell and the sperm which have foreign DNA. So kindly give me ans...
Can this process used human?
Thnks
My pleasure
You make it clear and easy for me🤝
Thank u🤍🤍🤍
My pleasure
Good but your explanation method is very fast so sorry did not understand on single word
Sir ak video hinde me bhi bta de
Bhai thoda sa aaram se bolo itni jldi kha Jana hai kuch samajh bhi nhi aarha baki Nice explanation 👍
sir please help me with retroviral gene transfer
Sure
Next topic transgenic anamils please sir tell me
Sure
kal exma hai isliye yaha aaa k dekhte🤣
Aap thoda dhere dherre samjhaya karo well done 👍
Thanks
sir hindi me explain Karo 😢
nots mast bhanaya hai ❤
Sir what is difference between electroporation and microinjection
Electrical voltage is used in electroporation for creating pores around cell membrane. And in microinjection you can see it in the video
Urdu mein ya hindi mein
Sir thelugulo cheppandi
Go to 5:07 for embryonic stem cells
Urdu mein explain krein sir
I don't know urdu
You are going to....
Ur words are very speed.
Little difficult to understand 😐
PK
Sir please explain in hindi
If I explain hindi... South indians can't understand... Hope u understand 🙂
@@bioscience6853 but sir
You can make this video in hindi or not ?
We are so many students of rajasthan have subject biotechnology and we are getting right teacher
I feel you are a best teacher so please help us
We will be so much thankful of you
Amazing explanation