TISSUE CULTURE UPDATE 2023

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  • Опубликовано: 25 окт 2024

Комментарии • 37

  • @SXJAYSX
    @SXJAYSX Год назад

    I'm so happy for you and your Thai Cons! It's been great following up on your journey. You were so defeated in that one video but it's so worth seeing you succeed at it now! Go Stratum!
    I just put my baby TC in stratum and perlite two days ago. So far so good. Fingers crossed!

    • @leafletco
      @leafletco  Год назад

      Aww! Thanks for watching! I truly was defeated but I'm happy they are still growing and thriving :) sending all the positive planty vibes your way!!

  • @prismatica8416
    @prismatica8416 Год назад +1

    Monstera in general really don't like transitioning media. If you do your plant will die halfway before it starts picking up again. So yeah, pick a medium and try to remember your soil blend. I'd try to use the same brand of whatever you used if possible.

  • @HellYeaBizzleImonyoutube-hc6ts

    I bought a beautifully variegated Billietiae from tc and after 2 new leaves it reverted. About 7 more into it's life now and still no sign of it returning. Grown under Barrina T5 grow lights. I totally get the frustration 😢

  • @austinmesta9862
    @austinmesta9862 Год назад +1

    Don't confuse genotype with phenotype! A plant that's genetically variegated can make a few leaves with more chlorophyll if the total plant overall needs more photosynthesis. Once the plant has more leaves and green stems, it won't need to max out the chlorophyll content of the leaves and the variegation trait will show. Likewise most plants will grow a white root but if you expose that root to light for days, even the root will turn green. Another reason to have more chlorophyll is that is acts as a sunscreen until the plant forms enough carotenoids to do some of that job.

  • @ofcv1238
    @ofcv1238 Год назад

    1:33 your disappointment is still a proud insight for you & others so feel confident sharing. Also, I spent most of academic life in plant physiology and cell specimen propagation. I believe you should refer to you babies as “specimen” or “baby whatever” but not really “tissue culture” once moved from monoculture hermetically sealed environment toward other (I.e., seal broken).

  • @austinmesta9862
    @austinmesta9862 Год назад +1

    I had a pothos that was variegated for five years and I reported it into some very rich homemade compost and it grew a few green leaves just because it had the extra nitrogen and protein. It grew like four green leaves then it grew more variegated leaves after that.

  • @ofcv1238
    @ofcv1238 Год назад

    11:35 lol…i have been doing it since before pandemic AND your birth. Terminology fuzzy here. Three basic stages: 1) mono culture in hermetically sealed space (your “tissue culture” samples), 2) transfer from 1 and 3) 100% conditioned . The secret is getting carefully from 1 to 2 and fastest arrival to 3. Different techniques and too hard to explain here. Happy to explain but typing fatigue setting-in…perhaps reading fatigue for others too?

  • @Universityofjamon
    @Universityofjamon Год назад

    Stratum is most definitely the best thing since slice bread. Those Thai's look great!

  • @colorfulsomething5008
    @colorfulsomething5008 Год назад

    Hey! I'm a bit late... Amazing video^^ What kind of substrate is stratum actually? I'm not native english and struggled a bit when searching for stratum. I only know (and found) it as a volcanic soil underwater substrate for aquariums. It's sometimes sold for terrariums as well. Is it actually that which you're using? Or is it something different?

  • @miad.4976
    @miad.4976 Год назад

    Totally agree! All my thai cons in pon since tissue culture acclimation process,for bigger ones I added bigger chunks of pumice/leca/akadama but yeah keeping semihydro, they're really hates this transferring. Learned a hard way 😢😅

    • @leafletco
      @leafletco  Год назад +1

      Same! I find that if the plant has already really matured it just doesn’t look change at all 🤷🏻‍♀️

  • @greenhut1642
    @greenhut1642 Год назад +1

    Is it possible to use led light for growth of plants

    • @leafletco
      @leafletco  Год назад +1

      Yes you can! All my tissue cultures were under led grow lights

    • @greenhut1642
      @greenhut1642 Год назад +1

      @@leafletco Thanks for reply, how many watt led should I use.

  • @lauragoldsborough9974
    @lauragoldsborough9974 Год назад

    What’s your grow light intensity for TC?

  • @ofcv1238
    @ofcv1238 Год назад

    8:40 what vendor in Thailand does smaller volumes? I only know wholesale vendor.

  • @ofcv1238
    @ofcv1238 Год назад

    18:26 problem was hobbling your way to “2” in previos comment about stages & crawling along to 3. Soft walls of cups or movement in 3 dimensions is shifting substrate around and disrupting the earliest phases of root development. Transparent walls okay for monitoring progress but need opaque trays to eliminate light during incubation & vertical lifting only until you are at stage 3. I think YOU need to think of 2-6 oz sized cups to go from stage 1 to 2. Focus on technique and dispel perceived notions on what really are critical factors impacting results? I can explain if not clear. For example: substrate obviously not flying out of your vessel. However, the physics involved is forcing shifts and pressures that would be equivalent of earthquake on “earth scale”. The hairy part of roots are the billion square footage multiplier that gives a plant robust access to its environment. I am not positive this was harmful to your specific plants; just saying like at the micro-root structure of plants online…even an electron microscope level magnification…that plant architecture stuff DEFINITELY getting messed up while hobbling along through stage 2.

  • @MyLocsMyPlants
    @MyLocsMyPlants Год назад

    nice ! so I have a thai con and an albo that almost died on me so I finally was able to reroot them lol they're still sitting in moss I am so nervous but I think I need to place them in soil now.

    • @leafletco
      @leafletco  Год назад +2

      Lolol so long as you’re giving it nutrients it can stay in there. But if you’re adamant on putting it in soil you can make your soil mix and have some moss in there too so that it can transition easier (moist moss you don’t want to water log it)

    • @MyLocsMyPlants
      @MyLocsMyPlants Год назад

      @@leafletco awesome thanks ! keep your fingers crossed for me 🤣

    • @leafletco
      @leafletco  Год назад

      @@MyLocsMyPlants I sure will!!

    • @jvsings9146
      @jvsings9146 Год назад

      Where are you getting your TC plants?

  • @thankxvan7183
    @thankxvan7183 Год назад

    What soil mix is it in?

  • @thankxvan7183
    @thankxvan7183 Год назад

    I have a thai in stratum rn and it’s in a enclosed humid environment. It’s been doing well but should i water the stratum😭 the stratum is moist

    • @leafletco
      @leafletco  Год назад +1

      You should only be adding water when needed. If your stratum is still moist there is no need to add more water as you’ll run the risk of root rot. Hope this helps.

  • @ofcv1238
    @ofcv1238 Год назад

    3:52 i laugh at what your are missing: get yourself a black light flashlight and some other wavelength light sources…your leaves will explode with hidden colors you can’t see initially under traditional light sources. You will never have had as much fun in dark with your black light flash light in your entire life….

  • @Mstes2241
    @Mstes2241 Год назад

    I bought tissue culture thinking it's starter plant. I opened it and to my shock, "what the f is this"? Checked my order and It says, "tissue culture", lol!
    Oh goodness, I'm gonna kill it, wish me luck! 🤞

    • @leafletco
      @leafletco  Год назад

      👀🫣lolol no you’re not!! Sending you all the positive planty vibes!! Hope the videos are helpful and your TC grow and thrive!

  • @roseyreefs
    @roseyreefs Год назад

    Would you recommend stratum over a perlite moss mix? For taking them out of the baggies into a dome cup

  • @PlantSpam
    @PlantSpam Год назад

    Hopefully it comes out with maturity. 🫶

    • @leafletco
      @leafletco  Год назад +1

      I moved it closer to the light source to see if it will help!