What PF TEK BRF Jars Should Look Like
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- Опубликовано: 4 янв 2022
- In this video, I will show you what healthy PF tek BRF jars, and bacteria infected jar should look like. They may be covered with mycelium and smells like fresh mushroom, but they can still be contaminated with bacteria.
Great video. It's really helpfull to see actual examples of what it's like to grow mushrooms.
Wow ! Thank you, that's the Most Helpful video I've come across that shows the different stages of the Fail/Success process. I've had success and failure but sometimes just don't understand what went wrong !! . This video really helps break it down.. And if this is your first video many thanks to you.. Keep up the good work
Are you aware that metabolites and moisture are natural within the jars? I think your assessment of what contamination is is flawed. I did not hear you mention anything in regard to TEMPERATURE, possibility that some areas of the substrate could be compacted tightly and inhibiting or slowing growth, inequality of the LC injection at different points, etc, etc, etc. ALL of those jars looked perfectly acceptable. No trich, no black mold, no other contaminants
You still around ?
4:55 am what exactly is PF Tech? Is it grain or substrate. Then what do you do with it? Put it to grain or substrate? I use grain spawn, then either Hrain to Grain to expand out or to substrate.
The first jar just has more rhizomorphic growth. The other side of the jar and 2nd jar appear to have more tomentose (fluffy) growth and no contamination I can see.
If you are growing anything medical you may see a blueing or purple from the mycillion being bruised it's still ok to use.
yes please, more videos, you have awesome information!
Thank you for the kind words!
Thank you...an incredibly helpful video. I've been stalled in my efforts to start trying to grow because I'm uncomfortable not knowing what I should be seeing, and this helps a ton! Question on alcohol as a disinfectant/sanitizer: Does the introduction of a bit of alcohol at the inoculation point impact anything in a significant way? If not, it seems we could use a cotton ball soaked in alcohol on top of the port as an additional simple sanitary layer that the needle passes through. Thanks!
Very helpful video. Thank you!
Super helpful video! Seeing the uncontaminated mycelium growing out in a perfect circle was a big eye opener on what it’s supposed to look like! All my cakes are fuzzy white and non uniform which I was concerned about and this proves me right. Good to know I can still try to fruit them and hope for the best!
There is two different types of mycelium growth. Let it colonize and smell it. It should smell earthy like a mushroom. Usually if your jars are filled with super nutrient rich food, the mycelium is fluffier and less aggressive because there is plenty of nutrients.
I've grown successfully plenty of times, and it's not always circular. Also depends on the type of mushroom/mycelium.
Thanks a ton!
I was reading about how the rhiz and piss could also mean bacteria. You can’t always be 100% positive with every specimens growth.
Thank you for the info!
Thank you for the kind words!
I think the bacteria is coming from the spore liquid or liquid culture you used my friend.. I have seen this before. It is growing where the needle inoculated
This is why we ALWAYS get the needle red hot with the lighter between each jar.
I agree. This almost certainly a contaminated spore syringe. Had one happen to me a while back. Its obvious when it happens especially for me when I had had something like 50-60 jars all the way to fruit without any problems until six or seven jars all from one inoculation had contaminates. Pissed me off. When you spend as much time as I do to make SURE that doesn't happen and then its introduced with the spores themselves from the vendor....vendor is not around any longer and went out due to LOTS of contaminated spore syringes.
Absolutely
The biggest problem is not being able to tell the difference between white mold and white mycelium. White mold likes to hang out in the same places and doesn't mind indirect sunlight. Tends to be fuzzy compared to spider web mycelium, but then again mycelium can look fuzzy too. So it's frustrating, not knowing what to try to kill off, and what to leave alone. Good to note that you can still harvest with a little bit of contamination, at least on the second flush, I was able to harvest 5.5 grams. Better than nothing.
Spray with hydrogen peroxide. Kills the "spiderweb" mold. Not the shroom.
I got like 8 grams with less than a pint of bacterial oat spawn that took months to colonize (about 3/4 of the jar colonized) but I was able to throw the last 1/4 of the bacterial spawn in a small flower pot layered with potting soil letting it colonize and once it got small pins I threw in a flower pot Getting 2.5 extra grams out of it
Someone feel free to correct me if I'm getting this wrong, but... My understanding is that spore syringes have to be assumed contaminated because the mushroom the spores originate from was most likely not grown in a sterile environment and there's no clean up involved in their production. That isn't to say that they are contaminated, just that they should be handled under that assumption because there is a risk that they are. In contrast, liquid culture from a reliable supplier can be assumed to be uncontaminated (if handled properly) because it contains mycelium that was initially grown out on agar, allowing the opportunity to isolate a culture free of contamination.
sounds accurate
Wait so if I understand correctly we should spawn Trichoderma sp. cakes to contaminated bulk substrates?
Also, you want to make sure that when you put the alcohol on your syringe port, that it dries completely before you inoculate, because the alcohol will not kill the bacteria, if the alcohol remains wet and then gets pushed inside where all that moisture is, it will never kill the bacteria
Hey someone said that if from spawn to sub it doesn't break apart easily it's prob contaminated?? ...I found it doesn't break apart easily when shaking hard but it's all very white with no discoloration or any signs of contamination... is there another good reason why does break apart easy when shaking hard ...using oats
people have never thought to do one week of colder temp to prevent contam growth at its most vulnerable stage can also inoculate in 65 degree temp then move to 75 degree environment
I agree. This almost certainly a contaminated spore syringe. Had one happen to me a while back. Its obvious when it happens especially for me when I had had something like 50-60 jars all the way to fruit without any problems until six or seven jars all from one inoculation had contaminates. Pissed me off. When you spend as much time as I do to make SURE that doesn't happen and then its introduced with the spores themselves from the vendor....vendor is not around any longer and went out due to LOTS of contaminated spore syringes.
thats why you should put it to agar first no?
My cat liked it!
Can you use stale nuts as a substrate or a supplement to grow mushrooms? No shells or salt.
You can always try, can't hurt if it's properly sterilized
@@mycoweek8727 Right. I'm propagating several wild native strains of Oysters. So I can try the nuts in some, and have a control. Thanks for the response.
would you try to fruit those jars that look iffy? Maybe in a separate chamber?
I will fruit everyone of them, since it's just bacterial, no need to put it in a separate chamber. However, since it's bacterial, it could end up with mold more easily, so I would pay closer attention to the whole tub, and take infected cake out as soon as I see any mold growing on it.
First timer here. I have 2 bags of grain that are all covered with mycelium, however, the bottom of each bag is bare with no mycelium growth, does that mean there is contamination on the bottom of the bags?
Update please
No redistribute the grains by shaking it. It will eventually reach the bottom but a shake is faster to colonize it all.
Hey mate, can you do a video on your liquid culture / inoculant? Props if you explain the difference to me :D
IC, LI, they are the samething with living mycelium tissue floating around in sterile water. I will note it down as one of the suggested videos.
So how long after you birth the cake will it take to see pins in the fruiting stage? Great video by the way thanks.
Depends on substrate, type of mushroom, species, breed or "strain"(PS this is not a scientific mycology term, bu a term used by many psilocybin breeders). That said, your looking at about a month for pins, for most hobbyist mushrooms.
I would just do 4 holes a little bigger then the needle with a 1/2 layer of verm on top. Your growth will be much better and fewer contams . If the tek aint broke, dont fix it !
It sounds like you are trying to figure out why the bacteria in the last one is so bad, and there could be bacteria along with the spores in that spore syringe because they are inherently not sterile, so that may be why you’re seeing a little bit of bacteria here and there.
Have to burn needle to red hot to prevent contamination. Good lock.
I am Sub#2 ..w..question
Why do you have to sterilize the jar for so long?
How can anything survive boiling water for more than 5 minutes?
Extremophiles excluded
because it's a semi solid mass, heat needs to penetrate the whole jar, not just the exterior.
@@mycoweek8727 Great Video...quick question, after inoculating the BRF jars, I'm guessing there is no "shaking" the jar throughout the colonizing process (unlike standard grain jars) and just let the mycelium fully colonize the BRF jar before transferring to substrate?
@@Tony-dx3eo Yes. for BRF jars, you just have to wait after inoculation. Which is why I recommend "broke boi" tek for beginners, instead of PF tek. PF tek takes too long in my opinion.
@@mycoweek8727 Thanks for the quick reply! I've really been enjoying going through your video library..."mush' respect! 🙌🏻
how long till 500ml jar is fully colonized with mycelium?
4oz wide mouth the best to use.
WARNING: A few years ago my second batch of PF TEK M. Mushrooms was much larger than I could use for private use...and I had read that the potency withers after awhile. STUPIDLY I froze a large batch of Golden Teachers to have when needed...>I ate about a kilo and nothing happened....
@Gideon Pioneer I will answer your questions and I HOPE you answer mine! No, I remember why now I even did freeze them: When my spores arrived I had asked about the potency of them from the company, since they had arrived in my country from another continent and it took about 5 weeks to arrive..they wrote that the packaging was good and I might put them in the refrigerator until usage. Later when I realized I had more than I needed a large crop for my own use,and potency does go down of the mushrooms, I figured to freeze them until next use...and I did NONE of the above that you mentioned. I unfroze them and saw "mush" and no amount of mushroom and I ate a lot effected me and it tasted horrible. I would check about the variables you said on this great forum: but before i list it: I happen to talk to people named "Gidon" since the name "Gideon" is pronounced in its originally Hebrew "Gee-Dohn" and its meaning etc. My army brigade infantry was 4 smaller groups and our name was "The Boakim: and another unit was called "Gideon". Boakim kind of means "Smashing In" which we did in 1973 when Syria attacked us in Israel but 12 of my 27man unit died, I served combat from 1971-1990, and still cannot figure why humans need to attack humans. www.shroomery.org/forums/dosearch.php?terms=pf+tek May the Infinite One Bless You
Did you contaminate them? Were they sterilized?
I agree. This almost certainly a contaminated spore syringe. Had one happen to me a while back. Its obvious when it happens especially for me when I had had something like 50-60 jars all the way to fruit without any problems until six or seven jars all from one inoculation had contaminates. Pissed me off. When you spend as much time as I do to make SURE that doesn't happen and then its introduced with the spores themselves from the vendor....vendor is not around any longer and went out due to LOTS of contaminated spore syringes.
Pretty sure thats water with ,
Spores in it. Also pretty sure “syringe water” is not a thing.. like at all
And he was never seen again
I was wondering if there’s a way to tell if I killed my mycelium because I was trying to shake my bag to speed up the growth but I think I squished it and killed it…someone help
No. You can't kill it that way. It may stall a little while regrowing, but not killed. The only problem is if there's contamination, because you can spread contaminants with the mycelium.
Healthy mycelium will always bounce back so no need to worry
@@thispersonisblanked3457 well it had wet rot so it was not healthy and it in fact did not “bounce back”
Make more videos. Just speak.
Thank you for the kind words!
Ok sooooo this is just me but isnt your laze pointer putting out a technically bad spectrum of IRRays ? I mean couldn’t you just as easy use a pencil or stick or your mother in laws crochet hooks ? Lol
Syringe water??? Lol
spore syringes have water in them… lol
@@positivevibesveda Yup its called a liquid inoculate instead of culture and actually typically outperform liquid cultures. That's why people use condiment cups for agar then suck up a syringe of sterile water poke a hole through the top of the cup and blast the mycelium away until you get it all broken apart from the top of the agar and then suck as much of it as you can in the syringe and boom ready for some great inoculations. Works great for a larger grain bags especially over cutting and dumping cups of agar unless you have multiple cups of the same "strain" to dump into a larger amount of grains. The only time I wouldn't recommend it is if your grains are a little bit too much on the wet side.
@@POSSUM3K3 Sounds cool! Thank you for explaining
Doesn't know what he's talking about