How to Make Psilocybin with Yeast | Journal Club
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- Опубликовано: 9 окт 2024
- Psilocybin and psilocin are the active ingredients in magic mushrooms. In this paper review, I go over how researchers genetically engineered baker's yeast to produce these valuable compounds! The paper is titled:
Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives
www.sciencedir...
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W algorithm pull.
Right!?
@@BBWahooRight?
@@hippopotomostrosesquippeda5804
Right!
RUclips:
You don't normally watch this channel, but I'm pretty sure you'll like this one.
Me:
😃
🧐
🤔
🫠
Would be nice to have such "pulls" more often than once a freaking month tho xD
It’s all fun and games until you get a yeast colony in your gut and end up tripping 24/7
YO
@@DIYBiotech❤😂👍
nah unless you intentionally try to do it, giving yourself auto-brewery syndrome is pretty hard. The body becomes tolerant to this drug insanely fast too so I can't imagine the effects would really last all that long even if you did succeed
The transition to that being your base reality is wild to think about, its like when you're tripping, and you feel like you'll be tripping forever, except you actually do in this case...
@@alexrogers777 it still metabolizes into a lot of really nice things
I wonder if that would be a worthy trade, perfect, maybe too perfect serotonergic neurochemistry and never needing tryptamine containing foods again
but you will never ever trip again
Kegg of psilocybin got me tryptophaning.
What a time to be alive!!
straight up drinking it, and by "it" I mean, ahaha, let's just say, my psychedelic pilsner.
@@tissuepaper9962
Tryptophilsner~ Sign me up for a 6pack.
@@tissuepaper9962How was that trip
@@MacGuffin1two minutes papers reference 😂
we can design and release a highly competitive yeast strain that rapidly replaces all normal yeast in the world, and produces psilocin, so now bread gets you high, and bread will be like that forever basically.
Muhahaha
Most bakers use a single strain of yeast for each type of bread. The same scrutiny is exercised for beer and wine as well as mashes for spirits. There are other situations where it would slip on there. Sourdough bread is usually a local yeast bouquet from putting a dish of water with a touch of flour out, then when the tase is preferable, the "mother" batch is kept and regrown in a dish with a cloth over it to keep other spores out. Occasionally, they will split the mother and allow more local yeast to settle in. In that case, you could definitely affect a sandwich. Some fruits are fermented with local yeast as well to produce wines. If you have ever seen the video of the chateau elan reporter (ooh oooh ooh ooooh) making wine, you see her smash grapes with her feet. Think about that, your feet generally stay warm, dark, and often moist, making a perfect habitat for yeast to live. There are other places on the body susceptible to yeast reproduction. Think infections specifically in the downstairs regions. Now imagine a foot colony or other colony on your body producing a constant supply.
Now imagine how long it would take the general public to figure out they aren't just tripping over their feet, but tripping because of them.
Crazy
it probably doesn't get you high any more once your brain adjusts.
psilocybin breaks down at high temperatures
Given how competitive modern brewers yeast strains are, it would be pretty hard to engineer one that still gave good psilocybin yields while also producing the amount of ethanol and reactive oxygen species needed to outcompete wild and cultivated strains. You got strains that already go the yeast version of scorched earth and push 18+% alcohol/volume yields in their attempts at biocide.
Yeast is not just extreme pleasure. Yeast is the realization of immortality and the realization that everything is heaven. When you realize that, it's so good, it's infinitely good. It's not just good. It's good to the infinite degree. It's so much goodness that you can't contain it. your body can't hold it. Your entire body shakes and shudders in a cosmic orgasm of bliss. It's perfection. It's total peace. It's absolute love, that's truly yeast.
bros on something not onto something
I'll have what he's having
You just described that time I took 8 drops of acid not knowing what would happen 😂
Chametz essentially
This paper got me into biotech
I have never clicked on a video faster
Man I’m glad I found your channel. I’m a sophomore studying biochemistry and I’ve been thinking hard about what I want to study. I’m going to be binge watching your videos for inspiration. Thank you!
Your channel is litterally the best one in the biotech category tbh
Thanks!
@@DIYBiotechI was just thinking about doing this, but for salvia. The diviner sage I believe it's called salvanorium a
Commenting before this goes viral, already sent it to my mushroom buddies. 😂 great video
Legend!
THIS is alchemy.
You did trick me into learning. I tricked you into educating too. Good video.
I think you're right about the future where you can brew your own psilocybin in a jar, and I think that's pretty dope :D
That's how you grow mushrooms
For recreational use, it's already easy to legally get spores online and get to growing. This kind of stuff is more for mass production to enable low cost use in therapeutic settings.
Erm "in the future" ?? Anyone can do it now, just order the mycelium and a bag of grains as a kit, even a kid can grow these.
@@orion9k Well to get psilocybin you don't even have to grow anything yourself. Just go to your local park and look for naturally growing Psilocybe-species, like P. semilanceata. They are pretty widespread and contain high concentrations of those active compounds. Just remember to not eat any mushrooms that you're not 100 % sure what it is.
So yeah, getting psilocybin is not a hard task and anyone could do it.
But there is a big difference between growing your own mushrooms from mycelium and grains and putting some yeast in a bucket with water and sugar and getting psilocybin out of it. And last time I checked there wasn't any vendors offering these kinds of yeasts for sale, so brewing your own psilocybin is still a thing of the future.
@@theKashConnoisseur yeah that is the intended use for this tech, but when it escapes the lab to the streets and the ability to mass produce psilocybin is in anyone's grasp we can finally say that the drugs have won the war on drugs.
This is the best news I've seen in a looooong time!!!! ❤😊
maybe one of the best teachers ever
BTW - a bright red dragon told me getting the dose right is a bit tricky. A lot of plants produce DMT so maybe those pathways are known and that molecule will be next. As a Chemical Engr. I see DMT as better suited to extraction, purification, analysis, quantification and storage for the home craft brewers. Now you've got me thinking about how to process the Psi-brew. Thanks for this video, and congrats on your PhD.
isn't psilocybin already pretty close? 4-HO...
@@microfx Structurally yes. Purification, isolation & preservation require higher skills. DMT processing is a known art. Having said that it will be interesting if the yeast method gets out in gen pop and producers find a product form that meets consumer requirements. I'm betting the community works out those challenges.
Can you isolate dmt from animals more easily? I'm not interested in DMT as a drug I just am interested in the chemistry and stuff.
I'm not a chemist by any means but isn't DMT just melatonin with a small change? Couldn't it be like bought OTC .. acid or based or whatever to produce the DMT?
@@Superstar-nl5tl They are both derivatives of tryptamine, but DMT is closer to psilocybin than it is to melatonin. Psilocybin is just 4-phosphoryloxylated DMT.
I dont know who you are, but I have a feeling this is about to blow your channel up.
this will be your biggest video. wait and watch xD
You gonna get some likes with this one lol
Dude no joke he just summoned a whole neighborhood of communitys 😂😂
Valid comment
Tru
Already got recommended to me and I have no search history in any of that stuff lol
Well, you did trick me to learn about metabolic engineering.
So, if I understand correctly, you could use genetic engineering to produce a ton of every compound made by organisms? That is damn powerful.
Brewers yeast will reduce tryptaphan to tryptaphol as it is, then put an enzyme coded to attach a dimethylamine tail on it. At that point you got dimethyl tryptamine. For psilocybin you would want the enzymes from the fungi and increase the free phasphate added as diammonium phosphate to push the equilibrium toward the production of psilocybin.
holy fuck brother i have been into this subject for years and you just blew my mind,. thank you for your dedicated work, kudos to you
Taught me more about biotech than any others, don’t usually comment but this is a really great channel, I would pay for a course!
Much appreciated!
so what you're saying is: "I want large quantities of psilocybin" 😂
@@microfx😂
Magic Beer would fly off the shelves in stores! One beer per gram would be perfect.
😂you taking some weak shrooms, or you drink like a Muslim😂one of the two, cause a beer and a gram of shrooms are not equivalent. Put like 0.3 in each
Probably a bit high, that would be like a 5 gram hero dose of dried shroomies. I think 200-250 mg per beer would be perfect.
@@IronTeddyBear I think you're off by an order of ten. standard is around 10 mg Ps/ gram.
@@IronTeddyBeari don’t think 25gs of shrooms would be fun especially per beer
The idea is really crazy. It is amazing they figured this out. The yeast is literally just eating sugar and making the end product?
yea exactly! Imagine if people had this in their kitchens...
Woud be better to supplement with tryptophan and ammonium dihydrogen phosphate and other stuff to push the equation more in favor of psilocybin synthesis.
Very very nice video, it's not easy to learn BioTech (or not as easy as many other topics) by yourself and you made a amazing job explaining this paper. Had a little ChatGPT window to get some definitions or insights on things you mention I didn't know or remembered.
Also 100g equivalent of dry mushrooms in a 1L bioreactor is insane !
Glad it was helpful!
Awesome video
I had theorized that this was possible along with THC and CBD pathways in alge, but thank you for fleshing this vague idea out. Great stuff!
THC/CBD video may be coming soon...
@@DIYBiotechwow really? So honestly could you tell me how viable this would be for a home lab?
@@microfxyou might wanna look into conversion ofc cbd into thc, which is really easy imo
@@DIYBiotechthey already do cbd and THC. I forget the name but one of the companies that does it is out of Québec.
Yup it does work as algae have the right hardware for efficient biosynthesis of lipids.
can’t wait to take a 4 year biochem degree to spend 4 months genetically engineering the thing that makes bread rise to see shadows laugh at me
I think I heard that it is also possible to make opioids in a similar way. Imagine having a cellar full of psilocybin, opioid, and good old fashion beer. Find some coke yeast and the party would never end!
woow, this knowledge is applicable for so many biochemical synthesis, thank you so much for sharing!
There's a paper from around 15-20 years ago on making hydromorphone from yeast that I've been waiting to see actual usage. A video on that though similar would be interesting too. The paper was widely reported at the time but I haven't heard anything since.
opioid production in microbes has had a big push recently, but as far as I know, titers are still in the ug/L range, which may make the process not very economically viable yet. I did find some older papers on opioids and microbes, one where they fed a bacteria morphine as the sole carbon source? If you have the specific paper you want me to look at, shoot me a message or an email!
@@DIYBiotech I'll see if I can find it, I think they were starting with sugar.
i love where this is going
Imagine being able to do this for certain central nervous system stimulants, looking forward to that haha
imagine shaking a jar and then harvest enough material for a month quickly
@@microfx😂😂😂
Walter White has entered the char
Mind, officially, blown 🤯. This is game-changing. Im going to have a hard time sleeping tonight for sure. A lot to think about 😁.... Hahaha yes you did trick me with drugs to learn about metabolic engineering and yes it was interesting for sure😂 great video!
THC in yeast video tomorrow ;)
Bravo.......metabolites.......im backyard scientist........new content for thought.......in process I make a lot of brown liquids........metobolits.......form mycelium sweat....ur paper gives me cross references and gives me new direction .......I lack testing facility's....or haven't build them yet.......self funded.......I don't publish anything........cheers
Oh thankyou im interested ill watch this video later and again
This is really great. Thanks!
This yeast culture would become popular, for home use.
You wanna talk about something that has MK Ultra potential? This is it.
I was able to do telekinesis through lsd
@@thisguy9993 I time traveled on salvia and the second I had a thought of using it to MY advantage I shot right back. Says a lot about universal morality in my opinion. Like if my first thought was helping humanity It might have let me stay, or at least a little longer. Till this day I'm ashamed I was selfish in that scenario. Made me a better person.
Nice video to ho with a few others out there. Opioides grown in yeast. Then this. I expect to see some soon on THC in yeast and coca and other social drugs in yeasts. From biofactories to genetically engineering enable plants and fruits and such. Thank you for sharing the video. A good chunk of chemistry in them all.
THC in yeast video tomorrow ;)
highly fascinating. thank you for sharing!
Glad you enjoyed it!
Phantastica. Kind of science I dig. 👎 😎 👍
This is awesome science 😎
If you could, you would, and if you can, you will. A great time to be an explorer. I'll have a kykeon on the rocks please.
Bookmarking this channel to come back for a status update on his psilocybin synthesis journey and recruitment of willing beta testers 🙏 aka underground human trials..🤔
Alright alright you don't have to twist my arm, ok I'll do it. I'll pioneer the front genetically modified mutant mushroom sludge
STFU it's my fantasy and I'm allowed to have it
someone should totally reproduce this result. for science of course
Yeah and make a tutorial for beginners how to do it at home.
@@lijuminati We're Breaking Bad with this one!
@@soumyobiswaslko or baking bread ...depending on the yeast and other ingredients 🤪
@DIYBiotech I'd like to see someone do this for ergotamine, which is a fungus derived precursor to LSD. Is it possible to coax organisms to produce chemicals that are made synthetically and normally not natural products?
Yes! And I believe ergotomine has been made in yeast before! Might do a video on that.
Can you please make a DIY tutorial for making galactosyl-lactose (aka beta-GOS) at home? Evidently certain kinds of beta-galactosidase enzymes under certain conditions can cause partial hydrolysis of lactose and also combining of galactose with lactose to form galactosyl-lactose. Beta-GOS is the best prebiotic in the market and the literature for improving lactose tolerance.
I'll look into this! Very new to me.
Is casomorphone addiction the reason people that are lactose intolerant won't give up on dairy? Similarly, if I had celiac, I wouldn't eat wheat, not look for more ways to manipulate it so I could eat something harmful, as a drug. Guess that's just me being too logical again, sigh.
If a "food" made me sh*t my brains out, I probably would take the hint and avoid it, but that's me.
Asimov was all about what could be done with yeast in his foundation series as well.
I wish there was a RUclips tutorial which is so easy to follow along that I don’t need to use any brain power :D
Ngl, the idea of not having to wait weeks for a mushroom flush is exciting
ho ho let the search for some special yeast begin.. guess its gonna take some time before the man catch up to the magik brewers yeast hehe
Can you do a video show of the lab steps/ techniques? I find this very interesting. I have questions like Can it be done using Petri dishes? Can you see with the naked eye when you have achieved each step? If not, what process can you use to know that you have achieved each step before going to the next step? Thanks for the great video.
This process takes years of training in genetic engineering techniques. Part of the reason why I made this channel is to show people some of them!
idk about psilocybin - but I'm trippin on how much research and just how the heck did they do it in order to produce the knowledge to build those data maps.
We stand on the shoulders of giants
psychedelic beer is the future, soon folks will be microdosing their microbrews
makes me wish i had finished my chemistry degree program sooo many years ago ~
“I’m comin’ for every enzyme” 🧛♂️
"This paper might be more helpful if you actually want to do this project."
> *Proceeds to chug from container that looks suspiciously like 1L in volume.* 😳
Thanks for sharing, shared.
Thanks for sharing!!
Magic beer.
Mmmmmm, beer.....oh....oh shit
Imagine if you got autobrewery syndrome with that yeast 😮😮😮
@@christopherleubner6633 Given how quickly tolerance to psilocybin rises, it wouldn't be much of an experience for long.
@@christopherleubner6633 One word: tolerance. Go watch a video of some maniac who wanted to trip on acid every day for a whole week😅 You end up with crazy doses to keep tripping after a few days. With psilocybin it's the exact same (they even have a cross tolerance). My guess: there will never be any symptom, even if you somehow manage to get autobrewery syndrome from this yeast.
Makes sense that they did it more easily in another saprophyte. I wonder if it opens the door for production in other unrelated saprophytic fungi, though brewers yeast has to be one of the most convenient.
Yep! Brewer's yeast is the most well studied yeast and thus the easiest to work with (also very high homologous recombination efficiency). I'll discuss a bit in my next videos about non-conventional yeast, but with recent advances in genetic engineering, other organisms are much easier to work with now.
@@DIYBiotech Yeah that high HR efficiency must make it an easy choice for this kind of work. Always nice when something like that appears in nature. Kinda like how P. Cubensis has the good fortune of being so easy to cultivate on so many different substrates. Little serendipities, I guess!
Gotta say though, in dissent with your opinion on the inclusion of Methods and Materials sections, I rather like seeing them, not just for more intensive peer review and replication, but for transparency and the democratization of science as well. Certainly a specialist might be able to infer the process from the rest of the paper or even contact the authors, but I like the idea of non-specialists being able to either replicate the results or concoct offshoot experiments based on the specific methodologies employed. I mean, we wouldn’t be able to have the “will someone try this at home?” conversation without it. Moving away from them feels like a “pay no attention to the man behind the curtain” moment, and even with the best of intentions still might come across that way. I’d think, especially given the current academic climate, we’d want to reduce that kind of academic stratification at any opportunity, even if the inclusion of that information may be redundant for certain parties. I’m interested in what your rationale is with regard to not liking them. Maybe I’m missing something?
so is this yeast gonna make its way into the ecosystem?? especially if as you say, it will probably eventually be available to regular people. suppose it could be outcompeted by other yeasts and other microorganisms but still, i feel like it would persist at some level.
Heavily modified yeast like this carry the burden of several genes that make them not competitive against wild yeast. Yes, this could be a concern, but it is highly unlikely. It is not genetically advantageous for them to be producing this compound!
I'm a junior at the University of Arizona studying plant sustainability and plant sciences I was wondering if you could go over a cannabis journal similar to this one.
Isnt this kind of like Ergot?
yea similar! I might do a video on ergotamine production in yeast
I think I'll just stick to foraging in the cow paddock. 🍄
That's nice and all but how do you purify it? You're going to have to separate out the product from yeast culture.
Do you purify with a large column? What kind of column do you use to purify it too? I would be interested in seeing a paper purify a small molecule product like that from cell culture.
Can you use an instrumental separation technique to purify it from cell culture in fraction pooling?
Like these are the things I'm curious about lol.
Yes this is definitely a concern! But there are plenty of extraction protocols out there, here's one: www.researchgate.net/profile/John-Casale/publication/19122936_An_Aqueous-Organic_Extraction_Method_for_the_Isolation_and_Identification_of_Psilocin_from_Hallucinogenic_Mushrooms/links/5a56465ba6fdcc30f86d4df3/An-Aqueous-Organic-Extraction-Method-for-the-Isolation-and-Identification-of-Psilocin-from-Hallucinogenic-Mushrooms.pdf
i got a general question on such engineered yeast strains: do they diverge from the engineered variant after a while? is there a contamination risk with wild yeast strains etc.? i have no idea about any genetic engineering/biotech topics, i just find it fascinating and also saw some simpler DIY stuff about it on the thought emporium which was interesting to see how it works in practice. i'm wondering because i've heard about these types of yeasts quite often but i'm wondering if it could ever happen that someone reproduces one of these papers and just starts selling this stuff 🤣or if that's unreasonable because it'll degrade too quickly
People could definitely make this and sell it! Yes, over time the genetics of the strain will change. It is not evolutionarily advantageous to have these bulky, irrelevant genes integrated, so the yeast will eventually lose them given enough generations. However, we can create glycerol stocks (there's a video on this on my channel!) of the yeast to basically freeze them in time. It's like finding an heirloom tomato seed from a 200 year old tomato or something. The genetics will be very similar to the first sell that had those genetics.
Ah yes, the yeast of thoughts and mind.
I haven't watch the video yet but the title makes me imagine somebody buying some beers thinking their just gonna get drunk but accidently ingest Psilocybin lol
sassy pic goes hard brother
@@sopeylive Thanks I drew it a few years ago
So should i make bread 🥖 pizza dough with brewer’s yeast, or is it more complicated
Im on a list again
We're all on there too brother
Subscribed!!😎👍
@nilered pls do this for SCIENCE!!
Make a video step by step and explain everything that would be great. Thank you.
Brewer's yeast and baker's yeast aren't that far off. (In some bootleg applications, it's substituted anyways.) Imagine that something crazy happens with the next slice of toast as well.
Can it work on other plants medicine 😅 the next wave of brew speed lol 😅
Yes! I got more videos on this coming!
18:50 so realistically you are saying it will take you at least 5 months to make that sketchy website.
Really interesting
Glad you think so!
Making this is keggs right now!!
Now were getting interesting.
This is cool as f. How do i get involved?
*holds up jar of yeast with a flower in it* - like this?
about time
Pouring sugar over the mucky areas, will update on progress
cool but its fairly easy to grow magic mushies from spore at home. rather see yeast that can produce things that have to be synthesized usually... 2cb, mdma, ghb, k, etc. is that even possible?
For some things it is possible! Just gotta find the right enzymes to catalyze each step.
I don't know, most of the chemicals you name are totally synthetic and don't occur in natural organisms. It would be interesting to be able to biosynthesize ergotamine, though, the precursor to LSD.
thats soo crazy...
The should bake some psychedelic bread
Even better, psychedelic pizza! With mushrooms on top, of course.
Psilocybin breaks down with increasing heat. it's pretty stable at room temp but at baking temps it's suffering thermal degradation.
The real question is if I brew my beer with this yeast, will I get psychedelic beer or have I just made like Psychedelic Tea?
How cool tyvm
Let's get the LSA pathway to make LSD 😊
I believe this had been done already! Maybe I'll make a video on this soon!
@@DIYBiotech no way 🙃😆 that would be very interesting. I just ran across this channel, but I have read about many similar efforts utilizing yeast including the production of ephedrine and some opiate precursors. It is a very interesting topic. Thank you.
Is it possible to forego the pathways leading up to Trp synthesis by using readily available retail 5-HTP?
Asking for a friend …
Tryptamine can quite easily be made from Tryptophan
W video RUclips. Very cool algo
man i love science and drugs
That tryptophan molecule is almost identical to melatonin might be easier to just start with one of those.
Theeeeeeeeeeeeeeeeeeeeeeeeen , how to buy the enzymes ?
Is there a similar experiment or process for DMT?
Yes! I believe it had been made in yeast. Might do a video on it!
@@DIYBiotech awesome! please do!
Sounds like Jesse needs his walter white 🕴
Orange peel oil can be concerted to delta9thc
Amazing chanel
4-ACO-DMT is better than psilocybin. It also metabolically converts to Psilocin -- but not completely - and is active on its own. It's better than psilocin.. And the byproduct of metabolically converting psilocybin into psilocin is phosphorous. which causes nausea.
i dont get why they put so much effort to produce an inferior compound. Its not difficult to synthesize 4-ACO-DMT and it's a more effective psychedelic. The cost of 1 gram of 4-Acetyl-oxy DMT costs around $120 - that's 1000 mg with the typical dose being 25-35 mg.
WAY cheaper than psilocybin. Yet labs are seemingly dogmatically spending unnecessary money and time just because psilocybin is what's in mushrooms without asking whether mushrooms know what's best lol
Are there any by-products in the 'brew' that need to be removed before it is safe for consumption?
probably the yeast since raw yeast would hurt the stomach and cause discomfort most likely if you filter it out its possible
We getting bread cancelled with this one