How do you use a Centrifuge? A step-by-step guide!

Can an Antarctic fish do blood doping? Learn how a centrifuge helped answer that question - and learn practical tips for how to use a centrifuge! This video focuses on how to use a microcentrifuge, but the principles apply to large centrifuges as well.
0:00 Introduction
0:17 What does a centrifuge do?
0:58 Scientists used a centrifuge to study blood doping in fish
1:34 How do you use a centrifuge?
1:49 WARNING Make sure the tubes are balanced
2:27 Pro-Tip: There are several ways to balance tubes in a centrifuge
3:03 Pellet and supernatant
3:21 Pro-Tip: Orient the hinges of the tubes outward
3:45 Questions you can answer with centrifuges
The Antarctic Fish research story is based on this paper:
J. Brijs, M. Axelsson, M. Rosengren, F. Jutfelt, A. Gräns, Extreme blood-boosting capacity of an Antarctic fish represents an adaptation to life in a sub-zero environment. The Journal of Experimental Biology 223, jeb218164 (2020). doi.org/10.1242/jeb.218164
Cartoon Illustrations by Michelle Lotker
Scientific consultation by Ashley Lakoduk
Images by:
Blood into capillary tube by Marek M - CC-BY SA 3.0
Hematocrit in capillary tube by PookieFugglestein - Public Domain
Centrifuge tubes by Kathy Zarilla with permission
Handyfuge video with permission from Manu Prakash
Learn more about the Handyfuge hand-powered centrifuge and efforts to bring electricity-free Coronavirus testing to resource limited areas:
web.stanford.edu/group/prakash...
github.com/ethanjli/handyfuge
This video is copyright Jayme Dyer, published under the Creative Commons CC-BY SA 4.0 license.
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[@darcysanders7082]

I can't believe I didn't know about you. You're bridging the lab to practice gap for me and its looking like magic rn

[@zizuzebra]

Well explained thanks great

[@chrstina]

I love how fun you make learning, thanks!

[@Sp-fe1eo]

thanks for this beautiful video...really a fun way to learn something ❤

[@mechanical_engineering_passion]

excellent teaching style

[@lorazam_]

this video deserves more than millions likes

[@YouTooBio]

☺️ Thanks!!

[@debjitbanerjee8512]

Please make more such amazing.

[@sorlos]

THANK YOU!

[@monaesmaeili9090]

Thank you so much

[@DeliciaTrini]

Love this! subscibed!

[@YouTooBio]

Yay! Thank you!

[@witgiz2868]

great video, i cant wait to buy one and separate my pulp from my orange juice

[@YouTooBio]

Well, that's certainly one application of a centrifuge! 😆

[@zizuzebra]

😂😂😂😂🎉

[@monishamitra151]

Is there any mathematical formula for balancing tubes

[@jonathansmithCrabAuthor]

Off-topic comment, but did you film those outdoor scenes at the Eno River? I'm pretty sure I've crossed that very spot!

[@YouTooBio]

Not the Eno, but good guess! The outdoor scenes were filmed on New Hope Creek.

[@user-dl9of5el2w]

lovely and sweet

[@fathiyahauliadarmawan4807]

Hi! How do you remove solid pellets from the bottom of the centrifuge tube?

[@YouTooBio]

I've never seen an application for a completely dry pellet. Usually you resuspend the pellet in a small amount of liquid - either a little leftover supernatant, or a new solution. You resuspend the pellet by pipetting up and down. If you have a particularly big pellet, it can help to use the tip of the pipette to "stir" the pellet into the resuspension solution.

[@GES188]

Wow 10 q I like it

[@abhikapase]

You are best Teachers Ever🔥🥲❤️