Column Chromatography Basics and Lab Procedure

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  • Опубликовано: 5 фев 2025
  • In this video I have discussed about what is Column Chromatography, what is its principle and lab procedure and How to perform Column Chromatography in lab manually step by step.
    First step is to choose the stationary phase based on sample.
    Second step is to pack the column, packing can be done by two method 1) Dry method 2) Wet method.
    Third step is to choose Mobile Phase
    Forth step is to load sample onto the packed column. Sample gets purified on the basis of polarity.
    Later we have also look in brief at isocratic elution and gradient elution.
    Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos.
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    #BaaYo #ColumnChromatography

Комментарии • 729

  • @BaaYo
    @BaaYo  Год назад +4

    To access notes and practice questions, please join our Telegram channel t.me/baayo_official

  • @nousheenjaweria384
    @nousheenjaweria384 3 года назад +247

    A night before exams and videos like this are beat combination for complition of syllabus must say flawless explanation 🙌🏻

  • @norinqureshi8031
    @norinqureshi8031 3 года назад +35

    I m in MSc Biotechnology ....Mam you are great ... I have done this topic only 15 mins for my tomorrow's exammm 🥳🥳...it's very easy to learn with full understanding explanation....keep teaching mam...and Thanks a lot

    • @abhinavsharma2457
      @abhinavsharma2457 2 года назад

      I'm a student of bsc biotechnology (final year) can you guide me ? I want to do msc in biotechnology too but i don't clearly know how I should get jobs after it as i don't think my parents would let me do a PhD 😅

    • @abhinavsharma2457
      @abhinavsharma2457 Год назад

      @ans piya kyuki bhosdike agr comment pdha hoo toh mai bhi biotechnology ka hi ho toh biotechnology hii dekhunga... Kbhi kbhi dimag se kaam lee lete haa zada oversmart nii bnte... Agr nii aa ra hoga kcuh smjh tbhi logi ki videos me comment kr kr puch ra na... Pr ni bc tumko toh cool bnane ki koshish krnii haa... Nihayti chutiya aur anpad lg re ho mt koshish kroo

  • @safwan7471
    @safwan7471 6 лет назад +80

    Best video explanation of chromatography on RUclips. From basic terms to the whole procedure. Much respect.

    • @BaaYo
      @BaaYo  6 лет назад +7

      Thanks so much Safwan for the compliment 😊

    • @ritesh6561
      @ritesh6561 4 года назад

      Yes dr Safwan true said

    • @-AnanyaMohanty
      @-AnanyaMohanty 3 года назад +1

      True

  • @miryaadein9098
    @miryaadein9098 4 года назад +10

    I'm from Bangladesh 🇧🇩 and I don't understand hindi.
    But I can understand your every vedio fully. You're the best teacher I have ever seen to make a concept clear. Thank you very much. ❤❤🇧🇩🇧🇩❤❤

  • @mawraakhtar7904
    @mawraakhtar7904 2 года назад +1

    Lots of love from from Pakistan ..ap. kam time ma zeada sy zeada cover krti hen ap ....ap jaisa chrmatography or spectroscopy ka teacher nhe dekhaa youtbe py....Allah apko hamsha khush rakhy or bht kamyab kry ..ameen

  • @Al-Imtiaz
    @Al-Imtiaz 4 года назад +2

    Best teacher ever on RUclips.
    Much love from pakistan😍

  • @manishsingh3629
    @manishsingh3629 3 года назад

    Ek puri unit complete krwa di aapne, itne km time m...amazing. All videos are awesome.

  • @SufiyanAhmed-h3p
    @SufiyanAhmed-h3p 3 месяца назад +1

    Thanks a lot ma'am for your explanation . It helped me a lot to understand column chromatography 😊

  • @MujahidKhan-qu6lv
    @MujahidKhan-qu6lv 4 года назад +15

    Your way of delivering lecture is outstanding I enjoy it and it clear my doubt thank uh so much♥️

  • @MotivativeAlakh793
    @MotivativeAlakh793 5 лет назад +4

    Your teaching technique Waw😲😲😲🤭🤭🤭🤭 super explanation mam thank you so much

  • @jaydeepdumadiya2464
    @jaydeepdumadiya2464 4 года назад

    Best video on internet.
    .Thank you ma'am ...
    God bless you...😇

  • @drxanandmedicinegyan6742
    @drxanandmedicinegyan6742 4 года назад +5

    One of the great lecture mam. God bless you and your lovely family

  • @prachi645
    @prachi645 4 года назад

    Watching this video before exam ... Explanation is best ....

  • @hamzatahir14
    @hamzatahir14 5 лет назад +9

    I have my pharmacognosy lab tomorrow and this lecture really helped me out. Thank you, ma’am. Your student from Pakistan 🇵🇰

  • @bhaskarsingh8517
    @bhaskarsingh8517 2 года назад +2

    Thank god I found your videos mam tomorrow will be my exam..and I have completed all the topics becouse of your videos..🌸❤️

  • @vishalkaundal914
    @vishalkaundal914 5 лет назад

    Bahut hi nice mam and easy way me btaya, jitna pta tha usse jyada pta chl gya

  • @MLT100
    @MLT100 3 года назад

    Ayeee ayeee madam apki speed boht teez raftar , but wow 🤩 boht zabardasht lecture han main ny presentation and exam kly apki video sy preparation kia thank you ma’am
    Healthy raho aur ase lecture upload karti raho 👌♥️

  • @aasiyamaryam
    @aasiyamaryam 4 года назад +1

    Best teacher ever😘....thnku sooo muchh mam

  • @Studyhub1221-t3n
    @Studyhub1221-t3n 2 года назад

    I love you ma'am 💖 Chromatography ka pura chapter revision ho gaya... Thank uuuu sooo much ❤️

  • @rajjaiswal5224
    @rajjaiswal5224 4 года назад +2

    All concept clear in 10min.,
    thank you mam

  • @ABC-dq4io
    @ABC-dq4io Год назад +1

    Thankyou very much mam

  • @Aroobaacademy
    @Aroobaacademy 2 года назад +1

    madam each point become clear through watching ur excellent presentation

  • @arunshelkeavs2918
    @arunshelkeavs2918 5 лет назад +3

    Thanks u mam..u help at last moment.. tomorrow is my exam..n i was totally blank on chromatography..ur way of teaching is excellent...no need to read book now...Ty.

  • @Kurrm
    @Kurrm Год назад

    Nice explination in very short time.
    Love from pakistan❤

  • @Ajaykumar-tr2nb
    @Ajaykumar-tr2nb 6 лет назад +1

    Best explanation in this topic on RUclips..👌👌👌👌👌👌👌👌

  • @mohammadtuha7957
    @mohammadtuha7957 4 года назад

    I am from Bangladesh.your teaching method is very awesome... ❤❤❤👍

  • @Cinematicdaily64
    @Cinematicdaily64 4 года назад +1

    Very nice understand this video 👍👍👍👍👍👍👍

  • @syedshahfahim8823
    @syedshahfahim8823 6 лет назад +11

    The way of teaching is very good mam..

  • @rehanakhtar4861
    @rehanakhtar4861 4 года назад

    best lecture ever.first time understand chromatography....

  • @smruti011
    @smruti011 Год назад +1

    Thank you ma'am

  • @tmko734
    @tmko734 4 года назад +3

    this video makes my concept , crystal clear... thank you ma'am

  • @shahidkhan-p5l9c
    @shahidkhan-p5l9c 6 месяцев назад

    Very good lectures mam thank you so much best for exam

  • @anishamehta5243
    @anishamehta5243 3 года назад

    Thank u
    With out showing practically u made it more.n more clear
    Thank u so. Much

  • @twinklechauhan8709
    @twinklechauhan8709 Год назад

    I usually never comment but how beautifully she explained everything related to the topic😊❤❤wow❤

    • @BaaYo
      @BaaYo  Год назад +1

      Thank you Twinkle

  • @imanulhaq9256
    @imanulhaq9256 5 лет назад +13

    Best teacher ever✌

  • @rushikesh2709
    @rushikesh2709 5 лет назад +1

    Kya Kamal ki teacher Hain ye

  • @haseebahmad1578
    @haseebahmad1578 5 лет назад +4

    mam you are suuuuuuch a genius. love from Pakistan.

  • @Chem4students
    @Chem4students 4 года назад +1

    Baayo is my favorite channel. your explanation is excellent. i started youtube channel by inspiration of your channel. bless me i did video on column chromatography

  • @priyav227
    @priyav227 4 года назад +1

    The best and understandable video ever I have seen

  • @aditi9413
    @aditi9413 4 года назад

    Best explanation mam. Your way to explain is best

  • @The_poetry_world0
    @The_poetry_world0 Год назад

    teaching like legend.... jai ho apki mam

  • @kamrankhankamrankhan4452
    @kamrankhankamrankhan4452 2 года назад

    All time best lectures so skillfull

  • @swatirajshalu9268
    @swatirajshalu9268 4 года назад

    Vvv nice ma'am .....excellent...this is best video. On this topic

  • @aliyasultana5444
    @aliyasultana5444 5 лет назад +6

    Your explanation was awesome ma'am
    Thank you so much

  • @Smartstudyoffcial
    @Smartstudyoffcial Год назад

    Best lacture keep it up easily samj agaya

    • @BaaYo
      @BaaYo  Год назад

      Thank you 😊

  • @mayurhucche7736
    @mayurhucche7736 2 года назад

    Very simple explanation of chromatography Thanks Mam,,

  • @thakurankit7159
    @thakurankit7159 4 года назад +2

    another great video ☺️🙌tyvm mam u r our dronacharya 😇

    • @BaaYo
      @BaaYo  4 года назад +1

      Thank you Ankit :)

  • @pkknowledge3174
    @pkknowledge3174 2 года назад

    Mam tomarrow is my paper and today I am taking your lectures that are really helpful for me

  • @EnThuSiasT111
    @EnThuSiasT111 2 года назад +1

    very nice explanation. thank you so much

  • @swati-q6m
    @swati-q6m 4 года назад

    Very easy way of explanation.Cleared all concepts

  • @surajprasadsingh1786
    @surajprasadsingh1786 5 лет назад

    thanku so much mam ........bht hi acha eexplain kiya apne sare doubt clear ho gaye

  • @khanmotivation3823
    @khanmotivation3823 4 года назад

    Mam your explanation method is too good

  • @sachinmarko4313
    @sachinmarko4313 Год назад

    Thankyou mam for your lovely explanation. It's very easy to understand

  • @HarshPatel-xn6du
    @HarshPatel-xn6du 4 года назад +2

    Your explanation is useful for theorical as well as practical knowledge purpose too.

  • @zpchannel8504
    @zpchannel8504 2 года назад +1

    Amazing way of teaching🧡🧡

  • @mr.rajput1832
    @mr.rajput1832 5 лет назад

    Tussi great ho ma'am.....this video is really helpful for me......

  • @rahulsahu4730
    @rahulsahu4730 3 года назад

    Love the way you teach mam ❤️👍🏻 lots of love ❤️😁

  • @shyamsingh9490
    @shyamsingh9490 5 лет назад

    Aap teaching ke liye hi bani Ho... Bhut khoob bhut accha padti ho

  • @NedaMAlam
    @NedaMAlam Год назад

    brilliantly explained 👍

  • @RajeevKumar-gv7fc
    @RajeevKumar-gv7fc 4 года назад

    You are great teacher.
    😊😊😊😊😊👏😍

  • @mansirajput7655
    @mansirajput7655 4 года назад +8

    Nice explanation without consuming much time.
    And the video describes almost everything.
    But i suggest to please add instruments used too in the end of the video..
    Name of the instruments are also frequently asked in viva.
    Although i love your way of explaining things this easily.

  • @dreamtobesurgen1948
    @dreamtobesurgen1948 Год назад

    Well explanation 🎉

  • @arvindswami5982
    @arvindswami5982 5 лет назад +1

    Such a nice method of teaching, n specially these rare topics with full explanation , salute to u

    • @BaaYo
      @BaaYo  5 лет назад

      Thanks Arvind 😊

  • @DhavalPatel-ml4fi
    @DhavalPatel-ml4fi 2 года назад +1

    Nice explain 😌❤️😍

  • @nisharawat664
    @nisharawat664 6 лет назад +1

    Ap bhut ache se explain krte ho madam thanku so much for help

    • @BaaYo
      @BaaYo  6 лет назад

      Thank You Nisha 😊

    • @nisharawat664
      @nisharawat664 6 лет назад

      Mam ap chemistry m or kuch bhi explain krana plzzz

  • @DigitalCreatorp90
    @DigitalCreatorp90 4 года назад

    Very Very nice mam explanation video ❤️❤️❤️✌️✌️

  • @shahwazalam7986
    @shahwazalam7986 7 месяцев назад

    Wow great👍

  • @AqsaSalamat-u3o
    @AqsaSalamat-u3o 5 месяцев назад

    Best all over

  • @naturalvibs600
    @naturalvibs600 2 года назад

    Easy to understand your method , thanks ☺️☺️☺️

  • @suniltiwari6321
    @suniltiwari6321 5 лет назад +2

    Too good......u r really good to explain well about any field...thanks for helping!

  • @birdrockon1065
    @birdrockon1065 6 лет назад +1

    Thanku very much mam aap k lecture bahot hi umda hain.

    • @BaaYo
      @BaaYo  6 лет назад

      Thanks mohd sahil 🙏🏻

  • @alllearninginmarathi3618
    @alllearninginmarathi3618 5 лет назад +7

    Thanks you so much.
    Best teacher ever...mam thank you so much

  • @basicstudy1644
    @basicstudy1644 Год назад

    Thank you so much maim aaj. Mera exam hai very very nice explanation ❤❤❤

  • @tanishasingh1737
    @tanishasingh1737 Год назад

    Thank you so much ma'am ❤🙌

  • @vishwajeetmagdum8223
    @vishwajeetmagdum8223 5 лет назад +5

    Wonderful teaching✌✌

  • @sadullahsadi3475
    @sadullahsadi3475 6 лет назад +2

    U are explaining very well. Love ur lectures Sadullah Sadi from Afghanistan. ❤️❤️❤️

    • @BaaYo
      @BaaYo  6 лет назад

      Thanks Sadullah Sadi..🙏🏻

    • @sadullahsadi3475
      @sadullahsadi3475 6 лет назад

      Maam may I have ur email address

    • @SnehaMunni
      @SnehaMunni 5 лет назад

      Plzz once visit my channel hope it night be useful for you
      ruclips.net/video/OKvgsEFId-A/видео.html

  • @snehasingh5658
    @snehasingh5658 2 года назад

    Best explanation 👌👌👌👌 mam

  • @saidurrohman5393
    @saidurrohman5393 5 лет назад +1

    Class me jo clear nahi huwa is video me ho gaya .. thank u ma'am

  • @shivanidigra4753
    @shivanidigra4753 4 года назад

    Best explaination ever

  • @gktech.4321
    @gktech.4321 2 года назад

    Very helpful video 🙏🏼🙏🏼🙏🏼✅✅✅❤️❤️❤️❤️❤️

  • @Mr.masochist_3755
    @Mr.masochist_3755 2 месяца назад

    You are superb mam 😊

  • @vaibhavkumar3894
    @vaibhavkumar3894 5 лет назад

    really,too good mam👌👌👌👌👌👌👌👌

  • @varshapatil716
    @varshapatil716 5 лет назад +1

    Very good teacing. I like it very much

  • @VJNIKS
    @VJNIKS 4 года назад +2

    Thank you for the simple and wonderful video ✌🏻

  • @mominaaamir8353
    @mominaaamir8353 2 года назад

    Very well and easily explained💯💯💯

  • @akshayshinde2028
    @akshayshinde2028 3 года назад +1

    Nice explanation mam

  • @romicarashid7060
    @romicarashid7060 3 года назад

    Mam thanku Soo much u have made things very easy for me God bless mam❤️

  • @Fabulousbiochemistry
    @Fabulousbiochemistry 2 года назад

    Thank you so much mam
    Your lectures are awesome
    Can't define in words

  • @poojapatel3761
    @poojapatel3761 Месяц назад

    I have exam in 1 hour and watching this video 😅 and obviously I'm going to do good in exam. Thank you.

  • @samiullahkhanniaziniazi5488
    @samiullahkhanniaziniazi5488 3 года назад

    Mam your voice is so clear and cute

  • @samikshakokare6408
    @samikshakokare6408 5 лет назад

    Mam aap bhohot accha padhathi ho. Thanks for teaching mam😊😊😊

  • @suryakantsonwane6514
    @suryakantsonwane6514 5 лет назад

    ma'am your all video are very nice and usefull for study thank you very much

  • @amitbhatt9841
    @amitbhatt9841 6 лет назад +1

    ty so much mam no word for u and ur effort are really great ....ty so much mam

    • @BaaYo
      @BaaYo  6 лет назад +1

      Thanks Amit, happy to hear that 🙂. If you liked this video, don’t forget to hit the like button 😊

  • @chandrabhagapadulkar2163
    @chandrabhagapadulkar2163 3 года назад

    I really like ur teaching mam

  • @manishachahar108
    @manishachahar108 6 лет назад +2

    And a big thanks for uploading this video ... Thanku once again

    • @BaaYo
      @BaaYo  6 лет назад

      Thank you dear Manisha :)

  • @dimpalsharma8530
    @dimpalsharma8530 4 года назад

    Amazing mam great work👍

  • @DoctorAhmadZain
    @DoctorAhmadZain 6 лет назад +2

    Thnku fr that... material... from Pakistan❤❤❤

    • @BaaYo
      @BaaYo  6 лет назад

      Thanks a lot :)

  • @alivia181
    @alivia181 3 года назад

    So nice explanation Ma'am. Thank you so much.

  • @aysha840
    @aysha840 4 года назад

    great mam for your nice explanation from Pakistan 💓💓💓