Mastering Thin Layer Chromatography Technique | TLC Hands-On Lab Experiment

Hi Friends, Thin Layer Chromatography is a quick and simple method for separating and identifying compounds in a mixture. In this video we learn about the concepts of Thin Layer Chromatography from basic to advanced level.
We have also performed the experiment in Rapture Biotech Lab using TLC technique to master the concepts with practical knowledge.
We also calculate the RF value at the end to fully grasp the mathematical calculations involved in TLC. Hope this video will help you master the TLC technique with all the concepts!!
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#tlc #chromatography #biotechnology

[@BaaYo]

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[@DaniyalKhan-gt5ol]

Ma'am please ,never stop we are with you .
Thank you very much for great explanation.
You are so down to earth person .
Thanks a lot .

[@BaaYo]

Thanks so much 👍🏼

[@subhalaxmi422]

Thanks a lot mam.......I don't have deep knowledge regarding biotechnology practicals......you are a life savier mam...

[@BaaYo]

Thanks 😊

[@24-ankitajagtap67]

Correction as sir mentioned in video ..polar solvents and polar components move fast but mam said correctly ...
The sample in polar in nature move slow because polar components have strong interaction with the polar stationary phase ,causing them to move slowly through the TLC plate
And
Sample is non polar ..move fast
This is because non polar components weaker interaction with the stationary phase in TLC, allowing them to move quickly through the TLC plate.
The reduced affinity between the non polar sample and polar components results in faster migration on TLC plate.

[@chemcatalyst..]

If you use a polar solvent as the mobile phase in silica TLC, the polar compounds will travel faster, and the non-polar compounds will travel slower. This is because:
- Polar solvents, such as methanol, acetonitrile, or water, can form hydrogen bonds with the silanol groups on the silica gel, reducing the interactions between the silica gel and polar compounds.
- Polar compounds can dissolve and move more easily in the polar solvent, increasing their migration speed.
- Non-polar compounds, on the other hand, have weaker interactions with the polar solvent and are more retained by the silica gel, slowing their migration.
By using a polar solvent, you're essentially reversing the elution order, making it suitable for separating polar compounds.
Some common polar solvents used in TLC include:
- Methanol
- Acetonitrile
- Water
- Ethanol
- Acetone
Keep in mind that the choice of solvent depends on the specific compounds you're trying to separate and their properties.

[@24-ankitajagtap67]

@@chemcatalyst.. thank u so much

[@AS-ws8nn]

Thank you so much Mam and Sir....Very helpful explanation....❤

[@look6300]

Really Appreciable, Thank you so much ma'am 💙

[@BaaYo]

thank you . please share

[@rashmipandey1305]

Thank you so much ma'am 🙇‍♀️🙇‍♀️🙇‍♀️🙇‍♀️
Want more videos regarding techniques

[@BaaYo]

Thank You Rashmi, will try to create more such videos 😊

[@panda5818]

Thank you so much ma'am. Apko main bohot phyle se follow kar raha huun . Apse padhke sab topic Cristal clear ho jata hyy. Aur theory padhne ke baad practical main zyada samj ata hyy . 😊

[@BaaYo]

Thank You 😊

[@divyatiwari8573]

Thank you so much mam ❤

[@muhhammadsaeed9879]

Mam upload more vedio about chemistry and chemistry experiments

[@devkumarsamanta8507]

Awesome session❤❤👍👍

[@BaaYo]

Thank you 😁

[@aranksharoy8415]

Thank so much mam its just amazing session

[@BaaYo]

Thanks 😊

[@motivational_infinite]

very nicely explained maam.

[@ZainabDalvi-q3d]

Thats a perfect explanation thanks mam suddenly it's was very useful for me

[@BaaYo]

@@ZainabDalvi-q3d thank you. Please share with your batchmates

[@wisewave28as058]

Ma'am you are doing a great job.... Hume kafi help rhegi apke is video se😊 we love you ma'am

[@BaaYo]

Thanks a lot :)

[@thepulak1757]

Informative video .. thank you so much ❤

[@yasaswinipadala2488]

Mam..ithni aasan se samajh gaya mam..Tq..

[@BaaYo]

Thanks for watching :)

[@beatstore6918]

Oh damn 🎉🎉🎉 wow kitna acha samjhaya hai mam aapne😊😊

[@BaaYo]

Thanks a lot :)

[@bsbk6065]

Thank you mam...aur bhi aisi tara vid chahye mam

[@BaaYo]

Sure, Thank You 😊

[@LabKiBaat]

50 Subscriber completed. Thank you everyone.

[@meenupruthi9890]

Highly appreciated step, thank you mam

[@BaaYo]

Thank You 😊

[@razorr1920]

Apart from the great work that you been doing since past years, which i have been following you with appreciation of your work and knowledge, I would see you as the next Kiran M Shaw in your domain. Apart from the knowledge that you impart towards biotech which drives the community by engagement, I think product or service based collaborative research would be great too.

[@BaaYo]

This comment made my day 😊, thanks for such kind words. 🙏🏻

[@aleenaahmad6784]

Glad to have u ma'am waiting for more such videos ❤️

[@BaaYo]

Thank you 😊

[@tahirsmedicalandbiologyvid4969]

mam is k through ham lysozyme ko egg white sy separate kr skty hein?

[@hafeezulhaq9920]

Mam kamal ka smjhaya.❤ Please PCR and its types aur baki sb common use hny wali techniques ki ak ak kr ky vedioes upload kr dian. hm studenta k liy yeh bht helpful hngi.❤

[@BaaYo]

Thanks for appreciating, we will try to upload more such videos.

[@shivamraja9978]

🤩🤩🙏bhut bhut Sukriya mam

[@BaaYo]

Thanks for watching.

[@debashismohanty7549]

Thank you ma'am ❤ it's very helpful

[@BaaYo]

Thanks ☺️

[@AnushaP123]

Really helpful

[@richalatu1349]

Waiting for more videos like this ma'am 🤩

[@BaaYo]

More to come!

[@jitubhatt69]

Madam near ftnir pr vedio banaye

[@kajalagrawal02]

Amazing mam👏🏻👏🏻😍 plz bring such more videos.. thankyou soo much mam😊🙏

[@BaaYo]

Thanks Kajal. Yes we will upload more such videos.

[@its_vikrant_here]

Thanku so much mam this is very helpful
Mam i completed MSC in pharmaceutical chemistry please suggest me job after this?

[@DaniyalKhan-gt5ol]

Superb

[@gulabsingh1621]

How to join this program/ rupture biotechnology

[@Bionika.]

Mam Microbiology se bhi related lab work procedure n detail plz upload kre ......

[@BaaYo]

Thank You, sure will try to create more such videos.

[@k.m.chavan]

Unknown sample hai. To mobile phase decide kaise kiya

[@vaibhavaghao3037]

Solvents konsa he

[@thakurankit7159]

Wah Ma'am new avatar mein 🙌

[@BaaYo]

haha thank u ankit. ye avatar kaisa hai :)

[@AstonishingBuffer]

Thankyou so much Mam ✨🌸

[@BaaYo]

Welcome!! Thanks for watching.

[@souviknandi464]

Very helpful

[@BaaYo]

Thank You 😊

[@AryaRathi-b1g]

Jo polar component rahega wo solute front ke jyada paas yani kam distance travel karega n kyuki silica bhi polar nature ka hai ?

[@BaaYo]

yes

[@shilpagawade9575]

Love u maam

[@BaaYo]

@@shilpagawade9575 love ❤️

[@aanchalgupta6139]

Jo sample ko dry krna h vo hot air oven m krna h ya phir open air m

[@vishantkumar8645]

Hot Air oven

[@muhhammadsaeed9879]

Please upload more vedio regarding chemistry

[@BaaYo]

Sure, we will try to upload Chemistry videos as well

[@rinkalgupta1373]

Thank u maam,

[@BaaYo]

Welcome :)

[@rinkiboora1399]

Thanku mam...

[@BaaYo]

Welcome 😊

[@shaifalisoni8479]

Mam plz share video on transcription nd translation

[@BaaYo]

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[@ujjwal_maurya]

Mam please guide me mam aapne class 12 th ke bad kon sa course ki hai 🥺
Mai ne abhi 12 th ki hai
Mujhe iss field me jana hai

[@gajanandbairwa6033]

Ma'am
Private research institute me job kaise receive kre

[@Xushdkd]

Maam aisa he kuch technique certification course bhi kijiyee❤

[@BaaYo]

Good suggestion, we will consider this idea.

[@Xushdkd]

@@BaaYo 🩷🤗

[@aniruddhabhowmick2771]

Mam yeh aapka khud ka lab 🧪🧫 hain kya?
Have you started a new company?

[@BaaYo]

No, this is Rapture Biotech lab.

[@jaysinha9500]

सर का आवाज बहुत कम है, वॉल्यूम फूल करना पड़ रहा है, 👂 फोन लगाने के पश्चात् भी

[@patel2209]

7:27 meto dry ke bina hi karta hu 30 second funk marta hu or ho jata he 😢

[@vishantkumar8645]

Mam jis lab me record krri h usi lab me mai work krta hu😊

[@BaaYo]

yesss :)

[@janak100]

Maim,I want to learn practical and i where to learn please suggest
Mera job nahi lag rha hea

[@dhanashrijitkar6181]

Apne to only one time hi sample load kiya h
College hme 10 times load karne ko kaha tha
Sahi kay h mam

[@BaaYo]

@@dhanashrijitkar6181 10 times kyo.? 10 different sample or one sample 10 times?

[@dhanashrijitkar6181]

@@BaaYo one sample

[@kuldeepraj7839]

🎉

[@muhhammadsaeed9879]

You teach very well as like you beautiful

[@shallowdark4185]

Mam aapki ki sound nhi aa rhi ahi

[@BaaYo]

Please try again, audio is clear in the video

[@bikashkumar-pz4sq]

Mam is type ki or videos lao

[@BaaYo]

Yes, we are going to upload more such videos.

[@patel2209]

Me to TLC without gloves karta hu 😅

[@patel2209]

Or mere sir to direct hi spots kar dete he without any drawing 😂

[@ritikpal7968]

😂

[@guillermoiturriaga7863]

No entiendo nada ..pero estás guapa 😘😘😘😘