Performing Thin Layer Chromatography (TLC)

We've learned a few separation techniques, so how about one more? Chromatography separates components of a mixture by virtue of their differing polarities, and thin layer chromatography, or TLC, is an invaluable technique that is used almost every day by any laboratory chemist. Let's run through the details of this indispensable tool!
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[@veronicaaguiniga8496]

As someone who has already performed multiple TLC experiments, I feel that this video will definitely help students who want to watch how to perform this experiment before actually doing it in class. This has the upside of the student focusing on the understanding part instead of focusing on how to simply perform the experiment. I also appreciate that this youtube channel highly recommends watching an in-depth video of TLC before watching how to perform it in lab.

[@somewhatuninterested8989]

Yep, your prediction is correct. We're learning TLC and CC in Orgo Lab rn at my uni and I wanted to watch how to do it so I could understand what was happening.

[@jameshahollomon4893]

THANK YOU SO MUCH! This is way more helpful than reading a lot of techniques from the lab manual!

[@dominoot2652]

same these lab manual descriptions are making me lose my mind

[@cactuslover5]

Exactly😂

[@arpita5517]

im watching your videos during my JEE prep to get better clarity for certain topics…thank you professor!!
much love from india :)

[@anubhutisingh5920]

I'm here for neet as well😂

[@manharisglitched]

@@anubhutisingh5920 Get it on guys 💪

[@ajinkyajadhao2674]

Hua kya fr clear?

[@arpita5517]

@@ajinkyajadhao2674 yes but i chose bits pilani over tier-2 iit's

[@Anish-IITP]

@@arpita5517 isnt it lovely the way it works for us

[@RashedMA_TKO]

Man ur such a god send. I literally have a TLC write up due tomorrow

[@syruponcereal]

Great timing. I’m doing this lab next week

[@heavenlysongs7307]

Yesterday was my chemistry practical exam
And I did chromatography very well

[@SaadAli-qv9nf]

Thank you so much Mr. Dave for explaining so well and simple.

[@hennascornerpodcast]

oh my god you're actually my savior thank you

[@bendfocuspro]

These are great. I am a sys admin for a lab and these videos allow me to ask informed questions of the chemists that work there.

[@Thaumius]

In addition, if you wish to reuse a capillary you can dip it in acetone to clean it between spottings

[@s.9417]

What about ethanol or methanol instead?

[@hatlessjet7802]

I love these videos. Thank you. This helps to understand things a lot. I found out that j tolan lied about how far away he was on the 1000 mile video.

[@seaacow6347]

Lol, did this lab a year ago. Have to do it again tomorrow morning for a lab. Needed to freshen up.

[@seyedmostafahosseipourmash9168]

Perfect and very simple while teaches all necessary info

[@KJT_izumi]

0:29 Slight glitch in the edit there! The intro popped in there for a couple frames.
...But it's something that doesn't take anything away from the video at all. Good work! :)

[@maxine_q]

And at 6:28 I thought my GPU was dying.

[@laanz]

@@maxine_q Bad news:

[@MuhammadRizwan-e7y]

What a mind to understand such an easy way

[@salmonorange]

Great video! Hope you can teach us how to use different stains to find out compounds that cannot be seen under the UV light.

[@kaushikumarihami1982]

marvelous!!! simple, but has all the elements needed

[@qzh00k]

Worked for the Jarrel-Ash division of fisher scientific for a bit. OES is interesting

[@elizz6599]

Thank you so much, your way of explaining things is wonderful

[@apurva845]

Reminds me of my Botany practical in college

[@simulation1037]

Great Video! word of caution though @4:16, as much as possible avoid using funnel on a graduated cylinder.

[@niloofarkh4779]

thank u so much , it is perfect

[@beverlylapierre9894]

SO HELPFUL!!!!

[@Sidistic_Atheist]

Why couldn't you have been my Chemistry teacher 45 years ago.. All this made total sense to me.
Proof, that there is NO such things as, bad students, only bad teachers.

[@NadaNaserEddin]

amazing video, thank you professor

[@shereea1823]

Another fantastic video loving this series

[@JntGrc]

Bless you, I am struggling in lab given that we have to write our own procedures. 😓

[@holgerharild1638]

Very good and easy to understand

[@Bludgeoned2DEATH2]

Can’t wait to get to HPLC and GC, my specialty 😊

[@vidcoyoyo3156]

TLC can be used quantatively by using different concentrations of the same standard, and comparing the sample spot size to the standards.

[@_soyel786]

My teacher told me to watch this video 😅

[@munavathtanuja4401]

😅😅😅

[@asnsikder9576]

😅😅😅

[@ShahinaShahi-vt4cq]

Meeto😂

[@chirag__kumar__]

Also

[@keynaan0239]

Same 😂😂😂😂

[@mahmoudsanad6447]

Excellent, but you should make many examples for differnt sustances,polar or non polar,in addtion you can explain column chromat. and HPLC.

[@dharanireddy1806]

you are awesome professor Dave thank you for this video

[@mahdinajm9310]

Keep the good work

[@samanthaperry7788]

So helpful, thank you!!

[@scp_at_iitb]

Thanks for this video

[@ridhafaizal2620]

Very useful👍

[@karishmasingha764]

Thank you so much❤🥺

[@waelfadlallah8939]

Thermofischer is my favorite chemicals supplier. In my lab setting i always order from them, they provide the highest quality amongst the different chemicals that i work with.

[@waelfadlallah8939]

@@bugsbunny852 funny bunny 🐰 😆

[@apoenzyme8085]

Great job man

[@sciencenerd7639]

good ol' thin layer chromatography
thanks!

[@jpnye]

Anybody know why the jar needed to be shut? I assume to prevent evaporation during the experiment, but I was wondering if there was a more specific reason. Thanks for the great video!

[@Joemamahahahaha821]

After Jesus turned water into wine he did a TLC to test its polarity against a store bought wine.

[@fukpoeslaw3613]

That's not in the Bible!
You're probably an atheist.

[@keypo790]

lmao

[@atifashhabatif8391]

💀💀💀💀💀

[@GarfieldAtLasganaParty]

😂😂

[@notveryrea1]

🤣🤣

[@sherlackholmesholmes8374]

Thank you sir

[@theh4sh]

Thank youu professor

[@shadali4264]

thank you

[@Danthebestman]

My guy you still make videos???
You are a determined man
Respect

[@pranavsingh5235]

thanks ❤❤❤

[@glennpearson9348]

How does this experiment work when gravity doesn't exist?
LOL! Just kidding! I'm enjoying these practical lab videos. They're taking me back to my organic and inorganic chemistry days in college.

[@wfemp_4730]

I'd guess that the capillary action would be increased in the absence of gravity.

[@glennpearson9348]

@@wfemp_4730 There you go, balancing forces and stuff. You and your "free body diagrams!"
;-)

[@wfemp_4730]

@@glennpearson9348 That's correct. I never charge anyone for my body diagrams.

[@glennpearson9348]

@@wfemp_4730 Well played!

[@redvelvet3479]

I have a qustion. What if the extract sample we have is semi-solid, how do we dissolve it? and how to choose the appropriate solvent for a sample that has never been studied?

[@صابرين-ص4ه]

You are great❤

[@Akaeru]

What solvent would you need for flavonoids from cabbages, professor Dave?

[@dmbms]

THANKS

[@hanniehmar8885]

Ahh fisher my number 1 MSDS source

[@aswathykrishna9157]

Nice video

[@nyamburamwikwabe-fd7lb]

But why in thin layer chromatography the sample spot to be separated is placed on the TLC just above the level of solvents. Why sir??

[@jjrc3105]

If it was initially touching the solvents, then instead of traveling up the TLC plate, the spots would instead dissolve into the solvent and you would lose the spots.
If it was above the solvent level, then as the solvent travels up the TLC plate, the solvent would instead carry the spots through the TLC plate, where the spots would then interact with the polar surface of the plate resulting in a slower/faster travel time depending on whether the spots are polar or non-polar.

[@marizarm]

thank you so much, it helped me a lot!!!

[@heehee4454]

thank you chemistry jesus

[@Jenny-gv9gt]

What did I do wrong if my spots are running with the solvent front?

[@draganmirkovic9377]

Thanx, can You show elisa?

[@infinitivez]

What's the purpose of capping the solvent and letting it sit, before introducing the plate?

[@colinprice712]

Letting the tlc tank become saturated with the solvent vapour means that the solvent doesn’t evaporate from the leading edge as it rises up. This slows down the rate of movement and the components of the mixture separate into better defined spots rather than streaks.
There are some times when this isn’t needed, so you can run the separation without tank equilibration.
Also, many of the solvent mixes used are not “nice” chemicals to have in the air…

[@jenniferwinkel4588]

Hi!! I was wondering why you should cap the chamber for a few minutes before using it? Is there a specific reason for that?

[@mnemonicallysathyan4339]

IT WILL EVAPORATE TO THE SURROUNDINGS

[@LordCaptainFather]

Thanks....

[@hananl.3147]

Is that a fly in the solvent?

[@jwl796]

Nice video!! I was so confused when I was doing this lab😢

[@mcchillx]

lovely video woow

[@ilmacademy5735]

Sir among Be and Ga which is more metallic element

[@ilmacademy5735]

@@bugsbunny852 yes

[@ilmacademy5735]

@@bugsbunny852 i know the answe...but list night a professor said something different...that is why....nd mind ur own business.....

[@rassimsimou1594]

Good

[@BhargavGajera-pd2mr]

why certain compounds are not visible in UV light in thin layer chromatography.

[@ProfessorDaveExplains]

UV affects conjugated pi systems

[@asaltysurprise4107]

Back to chem lab in highschool

[@OldBenOne]

Back at work today.

[@LucasPlay171]

Yo i could maybe use this to test for NBOMe/LSD/something else, cool

[@PBMS123]

6:40 the video gets super glitchy.

[@PBMS123]

is there a bug in your solvent?

[@penguiin12]

lol i noticed it too, a little gnat flew in there because of the acetic acid and died

[@youtubeuser206]

i use TLC to estimate the THC content of my cannabis

[@ProbusMihraban]

Cool

[@-JA-]

👍👏

[@Maymaverick2000]

Toto-Africa ahh guy

[@intrestedtonature3246]

Love from father of Pakistan 🇮🇳🇮🇳🇮🇳🇮🇳

[@salikkhan9579]

Haha
Nahi kya mila tumko ya comment karka

[@hammadkhazir8230]

By using such kind of language you are showing your intle t

[@VijaysunderChambat]

Hii

[@diffracted_sky]

ok da

[@guycoolSpore2]

Second.

[@alialmarbouai7457]

how may I cite u lol

[@riksstaden4927]

TLC the method✨
TLC the channel 🔥🚮 🤔

[@Snaomib]

4 sips

[@76ks]

شجابني هههه 😂

[@dipwannitadas9633]

Thank you sir