Performing Thin Layer Chromatography (TLC)
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- Опубликовано: 16 фев 2023
- We've learned a few separation techniques, so how about one more? Chromatography separates components of a mixture by virtue of their differing polarities, and thin layer chromatography, or TLC, is an invaluable technique that is used almost every day by any laboratory chemist. Let's run through the details of this indispensable tool!
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As a research chemist tlc is an important step in determining a reactions success. I actually learned tlc from your channel back a few years ago
As someone who has already performed multiple TLC experiments, I feel that this video will definitely help students who want to watch how to perform this experiment before actually doing it in class. This has the upside of the student focusing on the understanding part instead of focusing on how to simply perform the experiment. I also appreciate that this youtube channel highly recommends watching an in-depth video of TLC before watching how to perform it in lab.
THANK YOU SO MUCH! This is way more helpful than reading a lot of techniques from the lab manual!
im watching your videos during my JEE prep to get better clarity for certain topics…thank you professor!!
much love from india :)
I'm here for neet as well😂
@@anubhutisingh5920 Get it on guys 💪
Great timing. I’m doing this lab next week
Thank you so much Mr. Dave for explaining so well and simple.
Yesterday was my chemistry practical exam
And I did chromatography very well
Man ur such a god send. I literally have a TLC write up due tomorrow
marvelous!!! simple, but has all the elements needed
Great video! Hope you can teach us how to use different stains to find out compounds that cannot be seen under the UV light.
Perfect and very simple while teaches all necessary info
I love these videos. Thank you. This helps to understand things a lot. I found out that j tolan lied about how far away he was on the 1000 mile video.
These are great. I am a sys admin for a lab and these videos allow me to ask informed questions of the chemists that work there.
oh my god you're actually my savior thank you
amazing video, thank you professor
In addition, if you wish to reuse a capillary you can dip it in acetone to clean it between spottings
What about ethanol or methanol instead?
you are awesome professor Dave thank you for this video
Another fantastic video loving this series
Very good and easy to understand
Keep the good work
TLC can be used quantatively by using different concentrations of the same standard, and comparing the sample spot size to the standards.
Great job man
SO HELPFUL!!!!
thank u so much , it is perfect
Can’t wait to get to HPLC and GC, my specialty 😊
0:29 Slight glitch in the edit there! The intro popped in there for a couple frames.
...But it's something that doesn't take anything away from the video at all. Good work! :)
And at 6:28 I thought my GPU was dying.
Excellent, but you should make many examples for differnt sustances,polar or non polar,in addtion you can explain column chromat. and HPLC.
Thank you sir
Very useful👍
Thank youu professor
Reminds me of my Botany practical in college
Worked for the Jarrel-Ash division of fisher scientific for a bit. OES is interesting
You are great❤
My teacher told me to watch this video 😅
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Meeto😂
I have a qustion. What if the extract sample we have is semi-solid, how do we dissolve it? and how to choose the appropriate solvent for a sample that has never been studied?
good ol' thin layer chromatography
thanks!
What solvent would you need for flavonoids from cabbages, professor Dave?
After Jesus turned water into wine he did a TLC to test its polarity against a store bought wine.
That's not in the Bible!
You're probably an atheist.
lmao
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😂😂
🤣🤣
thanks ❤❤❤
thank you so much, it helped me a lot!!!
Anybody know why the jar needed to be shut? I assume to prevent evaporation during the experiment, but I was wondering if there was a more specific reason. Thanks for the great video!
great video, hope you do one one column chromatography (adsorption or flash chromatography) and explain how tlc can be used to effectively create an efficient solvent system for any purification work-up using the more common column chromatography.
Can a standard UV-C light (~253nm, for disinfecting) be used for visualizing plates at home? they are only like $10, compared to the mineral lights or light boxes used in labs which are hundreds of dollars.
when creating reference samples for the tlc spotting of known or unknown samples, do they have to be in aqueous solution or in a solvent, and does it matter how dilute the sample is?
How do you create an Iodine chamber for developing tlc plates?
Hi!! I was wondering why you should cap the chamber for a few minutes before using it? Is there a specific reason for that?
IT WILL EVAPORATE TO THE SURROUNDINGS
Thermofischer is my favorite chemicals supplier. In my lab setting i always order from them, they provide the highest quality amongst the different chemicals that i work with.
@@bugsbunny852 funny bunny 🐰 😆
Thanx, can You show elisa?
What's the purpose of capping the solvent and letting it sit, before introducing the plate?
Letting the tlc tank become saturated with the solvent vapour means that the solvent doesn’t evaporate from the leading edge as it rises up. This slows down the rate of movement and the components of the mixture separate into better defined spots rather than streaks.
There are some times when this isn’t needed, so you can run the separation without tank equilibration.
Also, many of the solvent mixes used are not “nice” chemicals to have in the air…
Ahh fisher my number 1 MSDS source
My guy you still make videos???
You are a determined man
Respect
Is that a fly in the solvent?
But why in thin layer chromatography the sample spot to be separated is placed on the TLC just above the level of solvents. Why sir??
thank you chemistry jesus
Good
Sir among Be and Ga which is more metallic element
@@bugsbunny852 yes
@@bugsbunny852 i know the answe...but list night a professor said something different...that is why....nd mind ur own business.....
How does this experiment work when gravity doesn't exist?
LOL! Just kidding! I'm enjoying these practical lab videos. They're taking me back to my organic and inorganic chemistry days in college.
I'd guess that the capillary action would be increased in the absence of gravity.
@@wfemp_4730 There you go, balancing forces and stuff. You and your "free body diagrams!"
;-)
@@glennpearson9348 That's correct. I never charge anyone for my body diagrams.
@@wfemp_4730 Well played!
Why couldn't you have been my Chemistry teacher 45 years ago.. All this made total sense to me.
Proof, that there is NO such things as, bad students, only bad teachers.
Nice video!! I was so confused when I was doing this lab😢
Cool
6:40 the video gets super glitchy.
why certain compounds are not visible in UV light in thin layer chromatography.
UV affects conjugated pi systems
is there a bug in your solvent?
lol i noticed it too, a little gnat flew in there because of the acetic acid and died
Back to chem lab in highschool
Back at work today.
Yo i could maybe use this to test for NBOMe/LSD/something else, cool
👍👏
i use TLC to estimate the THC content of my cannabis
ok da
Second.
Love from father of Pakistan 🇮🇳🇮🇳🇮🇳🇮🇳
Haha
Nahi kya mila tumko ya comment karka
By using such kind of language you are showing your intle t
شجابني هههه 😂
4 sips
First!
TLC the method✨
TLC the channel 🔥🚮 🤔
Thank you sir