Ion Exchange Chromatography | Principle, Instrumentation & Lab Experiment
HTML-код
- Опубликовано: 10 фев 2025
- This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram
This lecture explains about the instrumentation, principle of Ion exchange Chromatography.
Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule-including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column-a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions.
Article source: wikipedia
Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel !
Subscribe: / @baayo
HPLC Chromatography: • HPLC Chromatography Ba...
UV Visible Spectroscopy: • UV Visible Spectroscop... #BaaYo #Chromatography
👋😃 Connect with me on
LinkedIn 👉🏼 - www.linkedin.com/in/artirani/
Instagram 👉🏼- instagram.com/baayo_official/
Telegram 👉🏼- t.me/baayo_channel
RUclips: ruclips.net/user/BaaYo
You are blessed me as an angel for my 1st semester 😊😊
Congratulations 🎉
you and shomu are truly great teachers shomu does not have the flow but he have in depth knowledge which he can deleiver with ease but you have great consistency
Exactly.....
Amazing
Jai ho shomu bhaiya
In shomu biology classes, there are too much mistakes regarding the gene function. I think u have not seen
Who is shomu?
Just want teacher like u who explain the content every well and teaching style ..... just happy to watch this video india needs teachers like uh best of luck❤️
This consept is totally cleared with your class ma'am everyone need a teacher like you who can explain a topic clearly
I have always found the basics of chromatography difficult to understand until I saw this video. Thanks a lot ma'am
Avuna
Great
Much better than Puspendra Kumar
Hi
Do u have any notes of analysis
Ek br v focus khrb ni hua vdo dkhte hue... Concept ekdm crystal clear 🙏🙏
Thnku so mch mam ❤️❤️❤️❤️
N u very cute mam ❤️❤️
This topic is slightly difficult and confusing,I have also watched other videos and books,but your video is clearly explained... thank you for making these these videos....🙏🙏🙏🙏
Maim pehly me shomus biology se lecture sunta tha... But ap to the point aur effective way se lecture deliver kerti han... I am big fan of you mam
Thanks a lot 😊 and wish you a very happy happy new year
Funny Boss G 2 shomus biology is best okay!!
@@zainabkhan4871 yes
Thanks a lot...This is a very helpful and informative video for chromatography
Mai bhi
Thank-you so much ma'am 😊your explanation is great and very easy to understand
Thank You :)
I still remember the days in 2020 when i used to prepare for an exam by watching her videos for Microbiology exams...😊
Huge Rsepect from Pakistan. Thanks for your lectures by the grace of God you have a very strong grip on analytical chemistry and your way of giving lecture is fantastic..Thanks Very much
It helped me a lot., actually i started watching because you were good🙊😛later i started listening the concept and i got good marks in exam👍❤️❤️😍
So, ma'am I have my MSc instrumentation paper tomorrow, I do not attend my college , and was sure about getting low marks in this paper, but since morning after watching your videos on every topic in my course, I am assured to get good marks in my last paper also, I wish I top my college this semester also..😊 Thankyou ma'am..
Amazing explanation. Koi bhi bnda smjh jaye aap jo bhi smjhate Ho😍
Ys right
Chota phatakha Badha Dhamaka!!!
This Short video is full of information, any other tutor would Atleast take 2-3 classes to transmit this much information. You are amazing ma'am. Thank you ❤️💕
Apki sari vedios itni effective way me smjh ati h mam thank u so much apke jese jitne bhi teachers h youtube par, un sab ko thnk u ,puri bsc in microbiology youtube or books se sikh ke pasout ho gyi or ab msc me bhi ap hi kam arhe ho thank u so muchhh and lots of love ma'm ❤❤❤❤
Kl paper h mam... Or y a topic a rha h... Abhi tk mujhe y topic samjh nhi a rha tha..... Bt apka video dekhne k baad ab mujhe bhut acge s clear h.... Thanku mam
Mam I am big fan of your Chanel, because biophysics topics can't understand me but when I was started for looking your chanel, biophysics topics became easy now . Thanx mam
I works in separation of biosimilars using process AKTA chromatography....whatever you explained deserves a appreciation and huge respect
Yes, I enjoyed to watch this video about chromatography séparation until now I can give the ideas to someone lots of love mam❤
best mam👌🏻
You are such a great teacher ma'am.. You compiled everything in a very short video.. Huge respect to you🙏
You Are A Truely Masterpiece Of Biology..I am A BIG FAN of Yours....
Thank you so much Sayantan
Thank you mam
My all doubts is clear in this video.
It is very simple way to understood the ion exchange chromatography and any other topics
Thanks a lot ma'am 🤗🤗
I'm big fan of yur mam beacause aap sare doubt ko clear kr deti hai Thank you so much mam
Very good explanation mam..thank u so much..it helped me a lot...i watched ur all video..
Thank you ma'am for such video
I didn't know any thing about instrumentation and the only subject which was very difficult for me but then I found your Chennel and I watch all video regarding the chromatography and spectrometry and now it becomes my favorite thank you again ❤
Ma'am kafi achha explain karte ho.... Hum yaha kuchh students milkar apke videos dekhate hai hamesha.. aur sach me samajh aa jata hai easily... Ma'an kafi achha hoga agar aap JAM exams me aane vale topics bhi padhana shuru kare to...hamare jaise bachhe jo tuition afford nahi kar sakte unake liye badi help ho jayegi ma'am youtube pe JAM biological sciences ke topics clear karne vala ek bhi channel nahi hai.... Plz... Requested from 7 students... Thank you🙏
Thank You so much Suresh 🙏🏻, bohat achcha laga ki aap log saath milkar meri videos dekhte hain. Mai koshish karungi ki zyada se zyada topics cover karun jo JAM syllabus me bhi help kare, Many thanks to you and all your friends😊
This topic is quite difficult but after watching this video i really want to give u a respectable salute ma'am really impressive 😍😍😍😍😍
Chemistry samjh na tho chod hi Diya tha apne teachers k wajeh se🙂
Thank youuuu soo much for wonderful explanation❤❤❤
Ur teaching method is gr8.. i lik u soo mchh.. May Allah succeed u more & more.. u r sooo mchhh hard working... best teaching method
Easiest video I found for all Technics 👌
Ma,am aaj apki vedio dekhi.....aap bhut hi amzinggghh....smjhte ho reallly tq so much ma,am😘
Thanks so much Varsha 😊
Ur flow and way of teaching provide eternal pleasure thankyou ma'am ❤️
I was trying to understand this technique from notes but its very difficult to learn from that book.Thanks mam your way of teaching is very easy and simple.Thank you sooooooooo much again.
Thanks Arshad 😊
Really ma'am you are amazing i can not understand main concept of ion exchange chromarography but after watching your lecture it seems so easy for me actually i understand the concept thank you thank you sooooo much 🙏🙏🙏
No doubt very strong concept and very charming style of teaching
You read many books and pdfs but this video is amazing. Totally simple and easy to understand.
Respect and love from Bangladesh 🇧🇩🇧🇩
Thank you very much ❤❤
Very easy and helpful to understand for me.Very well explanation.
I pray to Almighty for your better future ❤❤
Thanks Mir
@@BaaYo Always welcome ❤❤
You are great teacher ❤️❤️ no one can teach like you.
Meam aap.. Akdum jakas padhati ho.., mind me fix ho jata h... Sb.., lekin hr baar mera mind diverse ho jata hai... Kyo aap Itni beautiful ho or cute b... Nd aapke hairs... Me to aap bolti h.. To aapke 💋 ko hi dekhta rehta hu..., Aap topic explain bhut Accha krti h... Aaj mere exam h... Or mene aapki vdo dekh hi exam dene jana h... Thanku and love u medam ji... Aap isi trh hme apne cute si smile ☺ ke sath padhati rhe.. 😊🙏😍👌📖
Thanku mam nice video
Mam aap k samjhane ka trick bahut achha hai aap kathin se kathin topic ko easiest way me batate ho
Thank You 😊
your lecture is THE BEST among this topic
I am pleased by your teaching . Thank you mam ❤️ .
Thank you sooo much for this❤❤❤ tomorrow is my exam and this topic was going from above the head... After this video i am able to learn it easily ..thanks for clearing my concept😊😊❤❤
Nice video mam
Sab samaj me agaya
Bohot hi badhiya
Aap samjate he perfect
Thank you ma'am excellent Technic of your teaching and 110%your way of the teaching.
Thanks you mam
Your content can be understood at once proparly as due to your understanding level is excellent and good voice clearty.
Really helpful for me
OMG , before I have so many confusion BUT Now after watching this video it's become easy to easy :: Thank you so so much mam ::
baayo is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
mam very good job
i am also a teacher and lab trainner in pharma lab
m being a teacher appreciate you bc yr convyng mthd z good
Uh are too good ma'am... Only bcz of uh.. I am clearing my concepts..
Mam your teaching method is very amazing and excellent 👌 soo thanks from Pakistan 🇵🇰
You are greatt ma'am........very wellll explaining skills 👍👍
Really mam so useful video for basics. Ty mam so much for this video 👍
Superb mam.. After watching your video I was clear about ion exchange chromatography. Types of ion exchange resins which i got confused even after reading books.. Thank you very much mam
The way you are teaching is superb.
You, your voice and your way of teaching all are beautiful and best
Its too much helpfull for me...way of
Teaching is best keep it up
So well explained in a clear cut way 👏
I just love the way you teach and loves you❤❤❤
I can't stop my self....to like subscribe and share this video my God too good content in effective time
Thankyou so much mam ..🙌
This is my saminar topic..🍒
Nd u helped me alot..💚
Thanks mam for explaining it in the simplest way, thank a lot....
Agar aap nah ho toh, Instrumental Analysis app jesa khubsurat aur haseen nhi hota. Thank you ma'am🙏💕
Concept clear in just 15 min
Thank you very much
Thanks for your explanation today was my presentation on this topic and it went very nicely
Didi aap bht acha pdhati h... Fail hone se bacha lia aapne hme.. keep it up you are great!!
perfectly explain i got it clear every concept thankyou so much
Mam ap bhut ache se explain krti ho.. every thing is perfectly cleared thanks ma'am 😍😊
Thank you so much Aaliya 😊
@@BaaYo mam you know pta nhi kbse ye chromatography mujhse clear nhi ho rhi thi pr jbse apki video dekhi everything is perfect 😊
Great , feels so good to hear that it is helped you 😊
@@BaaYo 😊😍
@@BaaYo mam ap electrophoresis pr bhi video bnaye...
Maam you are the best teacher i ever seee
Nice explanation & very useful video for study Thanku mam❤
Bahut achhe se samj aata h apke lecture me
Thanks Mahesh 😊
Mam your lecture is very easy and understandable
Thank u soo much maam u r way of explanation is perfect 😚😚
Mam aap ka way of studying bahut accha hai as to other females
Really what a amazing teaching style
How fantastic mam I just understand it completely 🤩🤩
IEC ab pani ki tarha easy ho gaya. thanks maam
Best......
Thank you for video Ma'am ...
God bless you...😇
Explanation was ofcourse awesome but ma'am you are really gorgeous 😍🌹🙊
Mai bhi yahi kehna chah raha tha 😁
Ma'am u have vast knowledge in pharmaceutical analysis ❤😊
Thank you Amrit 😊
@@BaaYo one request maam if you st pharmaceutical chemistry both med.che and organic those are also help us pls ma'am take my request 🙏🏻❤️
U r too good keep it up and all that the best for ur next vdo 👍👍👍👍👍
✨ Thank you so much ma'am for make a concept very easy ✨
I glad that i found ur channel
Are mam...kaha thi ab tak aap,,,, previous exam main iss tarah ki details information ki jarurat thi,,,,,,,par koi nahi der aaye durrst aaye,,,,,,thank u👍🙏
Such a great teacher❤
Maammmm youu make me survive ... thnkuu for deatailed videos
So nice method to teach 😊
Thanks Zain 😊
Your vedios are easy and informative
awesome teaching 👍👍
Your teaching is very nice...
I just love the way you teach ❤
Beauty and brain. Nice explanation.
Thanks mam 🙏 clearly understand ion exchange chromatography
U r amazing
U explaination is just perfect
Tq mam for this video 🙏🙏🙏