i don't think it's right for competitive ELISA we want to measure the ag not the ab in this video he is explaining a situation where he measures ab instead of ag
Very easy to understand but am wondering why would you capture the competed Ag. And how is the primary Ab enzyme linked. But have also read that there are different procedures for competitive ELISA. So I won't argue. All in all thank you so much for the videos. I really appreciate
Sir what happens if no antibodies are present and only the enzyme linked antibodies are attached to the antigen present on the microtiter plate. And colour developed..
In this video you said that two types of antibodies are used one label with enzyme while the other is not and they compete with one another. While I have read in research paper that enzyme conjugate antigens compete with sample antigen. And this is tha only difference between indirect and competitive Elisa. Kindly review it again and also guide me regarding this issue.
If you want detect particular antigen from the sampe, you to use conjugate antigens.. if you want to detect antibodies, the conjugate antibodies will be used
Why we are testing antibody instead of texting antigen in case of HIV ? We need to find whether there is presence of antigen or not .. right? Pls explain
HIV antigens are also detected.. in tha case p24 antigens are the target.. in intial weeks hiv antigens are dominant...but antibodies are not found... after 2 to 8 weeks antibodies can be seen
Sir, the enzyme linked ab s r taken from where? From the serum sample? Well the non enzyme one is taken from the sample and that's understood, now since we actually wanna know the concentration of the ab, so how can we link the enzyme to those ab s present in serum? Humein to conc. pata nahi hai, so serum se kitna ab lena hai... Kitne ab ko enzyme linked karwana hai.. I'm finding it confusing 😭
Enzymes linked antibodies redymade hoti hain... jaise blood group detect karne ke liye kits hoti hain.. waise hi ELISA ki kits market me available h...
if your looking at the ability for an antigen to outcompete the one with the enzyme...shouldn't you want the concentration of both antibodies to be the same? Or else obviously the one with the higher concentration is going to bind more
you do a whole bunch of wells each with a diff amt of enzyme (known and labled) and then you can just read the tray to see where it started outcompeting and quantify the amount that way
This is the clearest explanation of competitive ELISA i have seen thus far, thank you!
i don't think it's right
for competitive ELISA we want to measure the ag not the ab
in this video he is explaining a situation where he measures ab instead of ag
You really have good ability to teach 👍 Thank You for your effort 😊
THE ONLY VIDEO THAT EXPLAINS WELL, SIMPLE AND CLEAR!! THANK YOU SOOOOO MUCCCCCH!!!!
Absolutely, the best explanation. ELISA techniques have always been confusing for me. You really made it simple 😅😅.
take all my money man, you deserve it
Amazingly explained and cleared all the doubts through well explanation and the animation...Thanks A lot for this!!!!❤
Thank u sr
Good & easy way of teaching
Make more vdos for us on technique's
Thanks bro apny mayra concept clear kr diya
Contact for Personal or group immunology crash course (online)
saj.99999@gmail.com
Sir bacterial vaccins method of preparion video cheyandi plzz sir
Super and good explanation...
Wishes to Know the way that you makes these types of animated tutorial videos
Thanks bro u saved my brain from going insane
Exam saviour videos.
Very easy to understand but am wondering why would you capture the competed Ag. And how is the primary Ab enzyme linked. But have also read that there are different procedures for competitive ELISA. So I won't argue. All in all thank you so much for the videos. I really appreciate
Also the one that is being competed for is the one that is enzyme linked. Not the competitors
Very beautiful explanation.. Thanku so much for this clarification 🙏🙏
Sir what happens if no antibodies are present and only the enzyme linked antibodies are attached to the antigen present on the microtiter plate. And colour developed..
Excellent explanation🌹🌹💮🌺🌼🌸💮
Best explanation 🎉🎉🎉
Plz upload western and southern blotting..by the way..u gave a wonderful presentation...tqsm
Plz inform me if you get my mail
Great great great job....🔥🔥🔥🔥🔥🔥
Super, good explanation
Sir pls Blood group k doner or recipient smjhain wo nai aj tak smj aya kon kis ko day lay skta antigen or antibodies ka chakar easily smjha dain pls
Sure
@@sajidmicrobiology ok i will be w8ting
Well explained 👌🏼... mashallah
Dude, thanks. That was helpful.
Very clear and easy to understand
Thanks
In this video you said that two types of antibodies are used one label with enzyme while the other is not and they compete with one another. While I have read in research paper that enzyme conjugate antigens compete with sample antigen. And this is tha only difference between indirect and competitive Elisa. Kindly review it again and also guide me regarding this issue.
If you want detect particular antigen from the sampe, you to use conjugate antigens.. if you want to detect antibodies, the conjugate antibodies will be used
@@sajidmicrobiology ok thanks
@@sajidmicrobiology it really means a lot for me
That was amazing , thank you
Strange sounds behind the video scary
So helpful. Thank you so much
Why we are testing antibody instead of texting antigen in case of HIV ? We need to find whether there is presence of antigen or not .. right? Pls explain
HIV antigens are also detected.. in tha case p24 antigens are the target.. in intial weeks hiv antigens are dominant...but antibodies are not found... after 2 to 8 weeks antibodies can be seen
👍
Im subscribing ur channel
Why high concentrated win competition
the best explanation of competitive ELISA! thank you! 👏👏👏
I think I heard the call to prayer
Very well explained ...
Very nice 👍👍
Supar se bhi upar 👌👌👌
Thank you sir
Thank you for you effort ❤️
Thanx... whr r u from ?
You are welcome ,lebanon
Ok
Thank you
Plz check your mail
I had mail my syllabus to you
Nice
Awesome bhai.. thanks for this video. I made a notes out of it. ❤️
this is best explanation of competitive elisa on youtube...thank u so much
Sir, the enzyme linked ab s r taken from where? From the serum sample? Well the non enzyme one is taken from the sample and that's understood, now since we actually wanna know the concentration of the ab, so how can we link the enzyme to those ab s present in serum? Humein to conc. pata nahi hai, so serum se kitna ab lena hai... Kitne ab ko enzyme linked karwana hai..
I'm finding it confusing 😭
Enzymes linked antibodies redymade hoti hain... jaise blood group detect karne ke liye kits hoti hain.. waise hi ELISA ki kits market me available h...
Excellent Explanation
Thank you so much, sir 🙏
What exact reverse for antigen?
Can you explain it?
Nicee❤
If more colour more we affected ? Or less colour more affected with anti gen?
thanks sir
Whether ELISA and EIA are the same or different?
Short and clear Explanation Thanks sir!
Can there be equal concentration of specific antibodies and enzyme linked antibodies? And what happens if there is?
I think standard readings or graph are always there to compare
Interesting scenario. But you are going to avoid that by using more sample
Excellent Explanation Sir❣️❣️
Very low sound
Good explanation
thanks a lot ☺️
Very Impressive keep it up bro 😉
Nice job sir
👏👏👏
Thanks a lot!
if your looking at the ability for an antigen to outcompete the one with the enzyme...shouldn't you want the concentration of both antibodies to be the same? Or else obviously the one with the higher concentration is going to bind more
you do a whole bunch of wells each with a diff amt of enzyme (known and labled) and then you can just read the tray to see where it started outcompeting and quantify the amount that way
best explanation, thank you!
❤Thanks
Plz upload more videos on microbiology
Which topic ?
Thx a lot
✌️👌✌️
antigen is reacting with both bro whose conc. we detrmine
Damn man , you made it easy
👍👍👍👍👍💯💯💯
God bless u brother
Such a easy and clear explanation by u really u have a great way of explaining Keep it up
Thank you so much for appreciation
Beautifully explained the concepts
🫶🤲
Thank you❤️❤️❤️
Awesome!!!!!!!
Is Competative Elisa is also called as Direct Elisa.......?
No
Okk thank you sir
Your efforts are appreciated!
Thank you so much
thank u so much
Most welcome