Polycystic Kidney Disease: Knockdown of wnt5a Causes | Protocol Preview

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A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys - a 2 minute Preview of the Experimental Protocol
Liwei Huang, An Xiao, Andrea Wecker, Daniel A. McBride, Soo Young Choi, Weibin Zhou, Joshua H. Lipschutz
Eastern Virginia Medical School, Department of Medicine; Medical University of South Carolina, Department of Medicine; University of Michigan, Department of Pediatrics;
We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish.
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