From Smearing to Smiling: SDS-PAGE Troubleshooting Tips

Are you having problems running your SDS-PAGE gels? Discover the answers you've been looking for in this in-depth video on troubleshooting SDS-PAGE gel running issues. We break down six common issues while running your protein sample in an SDS-PAGE gel, including protein sample migration, smearing, and improper separation. For each issue we will provide analytical insight into what might have gone wrong, and then offer troubleshooting solutions to solve that problem.
Indications of Problems
0:16 - Smeared Bands
0:46 - Proteins Ran Off Gel
1:10 - Smiling Shape in Gel
1:38 - Bands on the Edges are Distorted
1:55 - Proteins not Separated or Resolved
2:30 - Proteins Migrating Out of Wells
More Resources
Troubleshooting SDS-PAGE Gel Running Issues - goldbio.com/articles/article/...
Protein Electrophoresis Using SDS-PAGE: A Detailed Overview- goldbio.com/articles/article/...
Protein Ladders: Prestained Versus Unstained - goldbio.com/articles/article/...
Products
GoldBio Protein Gel Electrophoresis Products - goldbio.com/collection/protei...
Bromophenol Blue Free Acid, ACS Grade - goldbio.com/product/3722/brom...
BLUEstain™ 2 Protein ladder, 5-245 kDa - goldbio.com/product/915/blues...
Tris (Tris Base) - goldbio.com/product/3414/tris
Glycine, USP Grade - goldbio.com/product/3237/glyc...
Written, Edited, and Narrated by Karen Pierce
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Gold Biotechnology, Inc. - www.goldbio.com
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