Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?
There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.
Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder
I would like to know what justification has to be provided to the USFDA for more bacterial count to the first 4 plates and reject them. Reason for fixing last dilutions to the fda
Same methods can we use for Drinking water or juice analysis? One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water. Thank you very much.
I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.
@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole
I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3 Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml The accepted count is
There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates
![Noob To Max With DRAGON REWORK In Blox Fruits [FULL MOVIE]](http://i.ytimg.com/vi/LnBMOinoOvA/mqdefault.jpg)
Very good explanation thanks a lot
very nice presentation continue with clear
Your videos are saviour for people like me who are not from pure microbiology field 🥹
Thank you so much.
Am blessed to have watch this❤
Thank you
"Excellent explanation."
Explained so clearly
Glad to know that it was helpful
Wow very useful video
Thank you for your all the videos.
Love you ❤️
Thanks
Wow it's Amazing and helpful❤
Thank you
Wow, thank you very much for sharing this.
You are welcome
Excellent, very clear and to the point presentation.
Glad you liked. Thanks
Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?
There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.
Very nice procedure thank you
Thank you
Thank you so much, well explained.
Thank you
Love you 😀🙃🙂😁😃 its so usefull
Thanks
Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder
Noted
Nice one. Thanks
Thank you for your excellent explanation
Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?
To avoid condensation of water vapour on the lid of the dish because the media is still hot
I find this content helpful as a student doing her project.
Your video's always awesome 😘😘😘
Thank you
It's really helpful thank you
Thank you
I would like to know what justification has to be provided to the USFDA for more bacterial count to the first 4 plates and reject them. Reason for fixing last dilutions to the fda
Can you tell me what is the standard method you use to do this procedure? Thank you 🙏
Fda bam chapter 3 for Total aerobic count
FDA BAM - CHAPTER 3
this is amazing thank you
Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?
Same methods can we use for Drinking water or juice analysis?
One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water.
Thank you very much.
Same method is for Drinking water or juice analysis.
We will make video for yeast and Molds and Magnesium analysis
best video 😍
Very clear explanation, really helped me alot thankssss 🥹💖
Very useful vdo
Thank you
very well explained
Thank you
nice peresentation
Thanks
can you help me with what TPC count should be considered in acceptable range
for frozen fruits and vegetables?
Hello ma'am, this is same for anaerobic bacteria also
Which microbes present in these PCA plates please tell me name of microbes
Please share the determination of ammonium acetate assay method
Noted
Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?
Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate
Why using phosphate buffer?
Can one use distilled water instead?
Sir, do you turn on the blower when working in the laminar?
Same doubt ..do you know the answer?
Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.
To avoid condensation of water vapour on the lid of the dish because the media is still hot
Can this procedure use to butter sample?
No
What is the procedure used to butter sample ? 😢
Please upload video for disinfectation using chlorine or which is suitable
Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?
Yes
Can we use peptone instead of buffer you mentioned
You can use peptone salt solution
Mam can I use this technique for khoaya,Chenna or any milk based sweets?
Yes you can
Does the same method used in industries?
Yes. You can use
sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?
Will make leter
Is this method used for testing shrimp?
Yes, applicable
hi. may i know the reference for this procedure?
BAM, Chapter 3
Can you please make video on MPN method for detection of Esheresia coli.
Noted
Can you guide me how to test tri basic lead sulphate testing method
Will upload soon
I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.
Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks
@@MicroChemsExperimentsthank you
Can you please upload a video about enrerobacteriacea (IS 17112 part ll
@@neethuaneethua941 noted
I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.
Could you please upload the quantification video of beta carotenoid from carrot sample by UHPLC?
Noted.
Can I replaced by nutrient agar media?????
You can but without any reference
Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths
Noted
Ma'am how to do antimicrobial activity of polymer films?
Same as shown in the video
@@MicroChemsExperiments polymeric film is solid
@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole
would you please make a video on the hanna ph meter
Already made. Link: ruclips.net/video/PRxJgmzd5jE/видео.html
I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3
Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml
The accepted count is
There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit
I loved it
Thanks
When we can use the words Absent and Less than 10 if there is no growth...and if there is no growth can we mention Zero or not...
How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?
Make report as
What if my acceptable counts is in the first dilution, will the formula be C1/1xd?
Yes. Please share your final results here
how many sample is done in the same time ?
Why we make two/more petri plates of same dilution ?
There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates
Can i use Nutrient agar and peptone water in cookies? Is it acceptable?
You can use and will get results
Of which sample you had calculated this cfu
Soyabean meal
Best 👌
Which medium should be used total viable count of milk
Plate Count Agar
Can I use plate count agar and peptone water? Is that acceptable?
Yes. You can use.
Nice
Thank you
In the formula, how did you get 1 and 0.1 please.
Thank you
The formula is given by BAM, CHAPTER 3
Why can't we take the average of trial 1 and 2 and consider it as Cfu ? Do any one know??
You can just average of two trials
please whose protocol is it?
Reference Method is already mentioned. Please check again
Which method use? Pls send the method name
Reference is given in this video
Can you pls tell the limit for total bacterial count in the case of biscuits
100CFU/g
Very easy planas
Thanks
Adrenergic receptors activators
Adrenergic receptors activators
could you please share the reference for this work, thank you
BAM chapter 3
@@MicroChemsExperiments thank you
Ky ap ye video hindi me nhi bna skte hain aur 🙏❤️
If there is a video that calculates the histamine level in a fish sample using the Elisa method, can you please provide the link?
No video was made on this test yet.
Can I analyze the total bacterial count in sewage sludge by this method?@@MicroChemsExperiments
How to convert cfu/g to cfu/ml
These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample
3.21 cfu/g is my reading
.: Then how many colonies are there
There are about 3 colonies in 1g of your sample.
How to perform B.et test
Please write the full name of the test
Use natural accent
Ok. Will try
thanks but you should avoid fake accent
3.21 cfu/g is my reading
.: Then how many colonies are there
There are about 3 colonies in 1g of your sample.