Well done. However, there are two issues in this demonstration. One is that it is not specified how much inoculum was added to each plate. I assume it was 0.1 ml, which is standard. Second, the calculated number of CFUs that you report at the end is not per gram of the original sample, but rather per 0.1 ml of the solution. To convert that number to g/ml, you would have to multiple the 8.9 X10^6 by the total volume in the test tube, in which the 1g of sample was added. Am I incorrect?
Actually she's doing pour plate technique in which inoculum is 1ml (which is greater than spread plate technique whose inoculum is 0.1ml) hence cfu/ml (= colonies on agar plate * dilution factor / volume of inoculum (in ml)) will only be colonies * D.F becoz denominator is 1. For cfu/gram we have to know the density of sample so that we can divide the cfu/ml with density to know cfu/gram.
Hi, I really find your video very useful. I hope you will notice me. I dilute my samples so that I can count the colony and treated it with disinfectant. Do I need to dilute again after treating it?
Very helpful and explained in easiest way ma'am... thank u ☺♥
Brilliant.. this has been quite difficult for me but watching this video made it very understandable... Thank You 😊
very well done. This has been the best video that I saw on this material.
Very well explained. Can you please discuss/ tell in the comment how the results are reported in terms of log?
Well explained. Thanks 🙏
Nice we support researcher batter then RUclips Gyan provider for CSIR-NET/GATE.
This is good... I learnt alot
What is perfect formula for total coliform if sample is diluted
Thank you. Great presentation 👍
Well done. However, there are two issues in this demonstration. One is that it is not specified how much inoculum was added to each plate. I assume it was 0.1 ml, which is standard. Second, the calculated number of CFUs that you report at the end is not per gram of the original sample, but rather per 0.1 ml of the solution. To convert that number to g/ml, you would have to multiple the 8.9 X10^6 by the total volume in the test tube, in which the 1g of sample was added. Am I incorrect?
Actually she's doing pour plate technique in which inoculum is 1ml (which is greater than spread plate technique whose inoculum is 0.1ml) hence cfu/ml (= colonies on agar plate * dilution factor / volume of inoculum (in ml)) will only be colonies * D.F becoz denominator is 1. For cfu/gram we have to know the density of sample so that we can divide the cfu/ml with density to know cfu/gram.
This was super easy, nothing like what the demonstrator said in our lab
Hi, I really find your video very useful. I hope you will notice me. I dilute my samples so that I can count the colony and treated it with disinfectant. Do I need to dilute again after treating it?
Hellow mam , 10-3 dilution containing petriplate in approximately how many colony will be formed?
hello, how did you count the 89 colonies?
How to count yeast and mould in water
3.21 cfu/g is my reading
.: Then how many colonies are there
i know that voice
Thank you so much for this video!
thanks a lot