Detection of Salmonella species in Different Samples (Part-1)_A Complete Procedure (ISO 6579-1:2017)
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- Опубликовано: 23 июл 2024
- Detection of Salmonella species in Different Samples_A Complete Procedure (ISO 6579-1:2017)
Detection of Salmonella spp. is a very important microbiological testing parameter for Food, Feed, Water and Environmental samples analysis. This video represents a detailed procedure of for the detection of Salmonella in a sample. __________________________________________________________________________________________
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Detection of Salmonella (Part-2): • Biochemical Tests for ...
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Related Tag:
#Salmonella
#Salmonellatyphi
#microbiology
Thank you for uploading this video.. and it's really helpful and understandable too.. please do video for detection of staphylococcus spp and vibrio spp.
Thank you. We will make video later.
Thank you so much, this video was very helpful
Thank you. Stay with us
Very on point and interesting video.
Much appreciated
We have to take pre-incubated plates pf XLD or BSA?
Thank u so much mam ...its very informative
You are welcome
Good job, I've got a cultured plate with distinct colonies and I'll need your help in identifying the bacterium and medium used. Thank you
Its a long task. Its difficult to explain here. Neverthless you can subculture your colonies on different selective & differential culture media like MSA, Cetrimide Agar, XLD, SS agar, Chromogenic Coliform Agar, MRS, MYP agar, etc. After that do biochemical tests of the selected colonies for identification.
Excellent explanation thanks 💐👍
Thank you
Very info .thanks
It's pleasure.
Hi! I just wanna ask if it is necessary to use both RVS and MKTTn for selective enrichment? as well as XLD and BSA for Isolation? thank you so much
Yes. Both needed for selective broth. And you can use only XLD for isolation
Tomorrow i wll face auditor in micro lab its very helpful to me thnkyo so muchh
All the bestttt
@@MicroChemsExperiments i will try to my best
Please,give us the link for part 2 and do the video for bacillus cereus
Mam what sample do you use?
Thanks a lot make more videos for E. Coli, and zeldahl test protein testing and show serial dilution also
Okay. We will make it. Stay with us.
Thank you so much. I try to find part 2 but cannot find. Please..
This vedio help me alot
Thank you. Stay with us.
lot of thanks
It's our pleasure...
thank a lot 😍
Most welome
Thanks for this video ! I couldn't find Part 2, are you going to upload it ? Thanks !
Already uploaded. Link: ruclips.net/video/afpSDDvdGMc/видео.html
Good job
Thanks
Thank you....
You're welcome!
hi your video is very resourceful but can we use selenite broth instead of RVS and MKTTn broth? and also another question, what is the name of this method used? thank you
Yes you can. Method reference is mentioned in the video title
Please make a video on Bacillus cereus as per ISO 7932:2004 and Staphylococci aureus as per ISO 6888-2:1999 If possible.
Ok we will work on it soon.
Thank u so much❤
You're welcome 😊
Waiting for part 2.
We will upload soon
Thank youuuu
My pleasure
Very useful
Thank you
What are the advantages and disadvantages of this method as compared to others detection methods?
1. Advantage: This method involves double enrichment steps which increases detection possibility and facilitates to detect Salmonella more selectively, thus this is the more accurate method compared with other methods.
2. Disadvantage: MKTTN media, frequently, becomes not available in local market and there is no replacement media of it.
Very informative. Can I use distilled instead of peptone water?
No. Peptone water is used for Non-selective enrichment. Non-selective enrichment can not be achieved by distilled water. So you must use Peptone Water.
Do you have video on testing of pseudomonas Aureginosal. Please share
We will make it soon
With the exception of RVS/MKTT, any proposed salmonella enrichment broth? Thank you
Not at all
Salanite broth
Can you name simpler tests for detection of Salmonella with close accuracy?
No other simpler method to be known with close accuracy.
Do you have a video for preparing xld agar plate? Because xld agar can not autoclave
We will recheck
Do you have a xld agar plate preparing? I don't know why my xld agar always in a liquid
Don't cover the lid of plate until medium is solidified
N make sure the agar is completely dissolved in Duran bottle while heating in microwave
This can be achieved when u see the medium is boiling clearly i.e bubbles formation.
Excuse me, may I ask?
What type of another sample that is easy to detect salmonella ?
Test poultry feces, that has the high possibility to detect salmonella
Thanks! Is part 1 already uploaded?
Both parts are already uploaded.
Pls why did u use 1ml of peptone in mkTTn broth and 0.1ml in RVS broth
Method said so to do that for the selective enrichment of Salmonella spp.
Well done my teacher.
Please can I use this method for pharmaceutical samples?
Yes. You can
Mam, what kind of sample you r using?
Animal feed sample
Wauu. This is really a good video. Can anyone share with me a full document where I can find medium types used to enumerate bacteria founds in food. Please help if possible. Have a nice weekend
We can't provide any document. We are sorry. Thanks for your appreciation
Plz read bam manual
Can please confirm 6:16 mints its 0.1 ml or 100ml its confusing
0.1ml (100 microliter)
Is it compulsory to use all the reagents mentioned?
Yes
Can we substitute XLD with SS agar
Yes. You can.
What ois the name of the sample taken for the experiment?
Poultry Feed Sample
Do we need to make 225ml of BPW in the non-selective isolation stage? Why not less than that?
Ma'am plz make a video on yeast and mould determination
Noted.
Why does solid foods
need 24hrs for incubation after homogenized with buffered peptone water???..Not only for salmonella detection but also for enumeration of TPC??
24 hours incubation in buffered peptone water is needed for the enrichment of salmonella. But this enrichment is not applicable for TABC or TPC
@@MicroChemsExperiments then what to do for TPC after homogenized with buffered peptone water???
@@istiakmostofa7167 ruclips.net/video/IlTnLEgivts/видео.html
What sample did you use??
Food Sample
how much broth we have to take in the test tube?
10 ml
i dont have a stomachercan i use blender?@@MicroChemsExperiments
What was the sample used?
Animal feed
Can I prepare buffer peptone water from peptone
You can prepare according to the composition of Buffered Peptone Water.
2:42 what sample is that??
food
name of sample ,?
Animal Feed
Part 2?
ruclips.net/video/afpSDDvdGMc/видео.html
Refrence?
Iso 6579
Good video but the method is time consuming.
Yes, you are right
E-coli o157 plzz selective Agar name
HiCrome EC O157:H7 Selective Agar Base, Himedia
@@MicroChemsExperiments thank you dear
@@ajitpatil2153 You are most welcome
@@MicroChemsExperiments hicrome coliform Agar ???????
Everything is okay, except the narration.
The speech is not clear.
Please work upon that.
Rest, I love your channel.
Thank you for your observations. We will try to improve.