Biochemical Tests for Bacterial Identification (Part-1)_TSI, MIU, MR-VP, Lysin, Urea, Citrate
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- Опубликовано: 9 июл 2024
- Biochemical tests are very conventional & popular method for the detection and confirmation of bacteria after isolation from any sample. This video presents a detailed procedure of several common Biochemical Tests (TSI, MIU, MR-VP, Lysin, Urea, Citrate) those are needed to confirm, specially, a gram negative bacteria according to ISO 6579-1:2017.
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Beautifully crafted and articulated video. Thank you so much.
You are welcome. Stay with us.
Ur explanation is really a great one and very useful for my research work....thank you so much...
You are most welcome
thank you....this video helps me alot in my open-ended lab
completion
Thank you
Great❤ clear explanation
You are awesome!! Thank you for sharing!
Thanks for being with us.
Nicely described... easy to understand. Thank you
You are most welcome
Masha Allah Superb Explanation. Thank you 🌹
Thank you
i dont have innoculation needle can i do this using Loop?
and also i have methyl red indicator directly how do i use it?
Excellent and clear definitions.. good effort
Glad you liked it
Very interesting, I’m gonna have to practice soon cos we have a upcoming practical exam about this
Best of luck!
This is very helpful thank youuu
You're so welcome!
Thank you I have understood 😍
You are most welcome
Well done.
Thanks
Hello, can I know what is the volume of media are needed in broth tube for the culture before biochemical test is performed?
It varies on type of media. Some are 5 ml per tube, others are 10 ml for each tube
Very nice
Thanks
Very good 👍
Thank you 👍
GOOD CLARIFICATION
Thank you
thanks
You are welcome. Stay with us.
Would you please prepare a video teaching how to calculate Measurements Uncertainty for any test. For eg. Moisture / FAT / pH etc.
Ok we will. Stay with us.
Great video presentation!, May i ask why is there a need to loosen the caps of the tube before incubating it?
To facilitate oxygen transport into the tube (to make the aerobic condition).
Thank you!
@@user-kq7ri6zd9t You are welcome
In the step three: pure colture - 01, need to inoculate the colonies from XLD to TSA plate. Which XLD plates need to choose? Those previously obtained from selective enrichment of RVS broth or MKTTn broth, or both?
Thank you for your videos and for your work.
Subculture to TSA from XLD is important. You should choose colonies all XLD plates (from MKTTN and RVS, both)
@@MicroChemsExperimentsI would have another question. The ISO does not specify the non-selective medium to be used, can alternative media to the TSA be used? For example plate count agar or yeast extract agar?
@@charliefasurf5098 You can use plate count agar
@@MicroChemsExperiments To confirm the presence of salmonella, all biochemical tests must be positive (in particular TSI, Urea and Lysin). Is it possible the tests give a positive result for colonies obtained from RVS selective enrichment and a negative result for colonies obtained from MKTTn selective enrichment (or the reverse)? In this case what would the outcome be?
Mam kindly givens in v cholera have whatreacion we can found in biochemical .
You can use these Biochemical Tests for Vibrio cholera too.
Mam I have 45 yr female from fever case patient blood culture after growth only bap only rod and after biochemical test indole- ,urease+,cit-,tsi yellow with black ,and mha have mucoid around the antibiotics disc uti have greenish colony can u identify pls
Sorry we can't interpret this way
Hello. If I want to test for E.coli, what media is suitable as sources of inoculum for biochemical test?
Culture your suspected colonies on Trypton Soya Agar, Nutrient Agar or Plate Count Agar. Then used colonies for biochemical tests
@@MicroChemsExperiments Can I use Chromocult Coliform Agar for smear and then streak on TSA agar?
@@islaserena1599 Yes. You can.
Where are you from?
@@MicroChemsExperiments Thank you so much. I am from Malaysia.
@@islaserena1599 Okay. Thank you for staying with us
i need your help for salmonella ,satph.aureus,e.coli0157,bacillus cereus for food microbiology analysis
We uploaded video for Salmonella and E. coli.
We will work on Staph and bacillus soon
Ok Thank you very much
Can you share your Mobile Number plz
Thank you very much, Madam.
May you help me...about Test method of Staplylocuccus Aureus or any video link.
Will upload soon
Are you a food microbiologist?
@@my11account222 Yes.
@@SirajulIslam-vp9gp thanks god, can i ask you some questions sir?
@@my11account222 ok
Thsnks,, so much enjoyed
Welcome
Hello can you tell me about LiM medium LiM stand for ?
LIM stand for Lysine Indole Motility
@@MicroChemsExperiments thank you for timely response
Can you recommend me
1 dilution Media
2 slective enrichment broth media
3 slective culture media for staphylococus aerus & pseudomonas ariginosa for food analysis?
Great, thank you. Can you upload detection test for salmonella species specific test ?
All of the test in this video are also applicable for Salmonella detection. ruclips.net/video/BGoueEDj6VQ/видео.html
@@MicroChemsExperiments Thank you, very informative. What are the sample I can used? I want to use swab from life birds and egg yolk to detect salmonella spp is it possible by using your protocol.
Yes. You can apply this procedure for swab samples.
@@MicroChemsExperiments great...I will design a project protocol based on the methods...it would be appreciated if I get some written notes about the whole procedure/methods with list of lab equipment and chemical/reagent. Thanks
@@md.mostafijurrahman7561 Sorry. We don't provide any document. Please make a note from our video. Thank you
Pls mam.. Post Listeria testing procedure ..in Oxford media ...🙏🙏
We will upload it by the next week.
@@MicroChemsExperiments Tq mam 🙏
@@rumisahoo6961 Stay with us
Mam can u suggest for cholera and salmonella confirmation biochemical test
This video is for Salmonella detection by biochemical tests. You can also apply for Vibrio cholera.
Y tsa? Y not direct ?
plz upload the link of biochemical identification methods for bacteria
Part-2 will be uploaded soon
Please upload the 2nd part
Soon
Kya aap muze salmonella biochemical test kaise karte hai pdf mail lar sakte hai.. ???
Sorry we don't provide any document.
plz,different types of water test parameter,iron,zinc,chloride,mg,fluoride etc.
Noted. We will.
@@MicroChemsExperiments waiting for ur drinking water test parameter&procedure.
@@mehedihasanrabbi342 Thanks for your patience
Please do a video on Aflatoxin b1 test for aqua feed or on raw materials
@@rakeshkumarsonti9319 Noted.
May I get Biochemical Test Part 2?
We will make video later. Stay with us
Genome sequencing video?
In future we will upload
If we pay for identification of bacteria how would these cost?
Please write us at mail.mic.chem@gmail.com
hello i am syed from bahrain medical lab
Welcome to this channel
Can you share your Mobile Number plz
free fatty acid definition,plz
Free fatty acids (FFA) are produced by the hydrolysis of oils and fats. Since FFA are less stable than neutral oil, they are more prone to oxidation and to turning rancid. Thus, FFA is a key feature linked with the quality and commercial value of oils and fats.
Wow
You made it not simplified
Not simplified? You mean hard? Opss sorry
Thanks...oh hellow...where are you now...please answer me
India
@@MicroChemsExperiments may apse bangla mai bat karsat ta ho
@@prince_ranaofficial398 No. We dont speak bangla.
@@MicroChemsExperiments okey...one question...What it used Selenite Broth chemical test of microbiology
@@prince_ranaofficial398 Selenite Broth is used for the selective enrichment of Salmonella spp.
E. Coli and other coliforms are inhibited in this broth.