Counting cells using a hemocytometer video protocol
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- Опубликовано: 7 фев 2019
- View all our video protocols with written procedures at: www.abcam.com/tag/video-protocols
Watch our cell counting video protocol that takes you through the stages of preparing hemocytometer and cell suspension, counting the cells and checking them for viability.
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6:04 If my calculations are correct, if you have a viable cell count of 200,00 cells/mL in a total volume of 20 mL, then that should be 4,000,000 cells. If you then want to to seed 10,000 cells into the new flask (0.0025 or 0.25% of the total cells), you should transfer 0.05 mL (50 mcL) of the original sample into the new flask.
totally agree
Plz tell me
Total procedure of platelets counting
Uhhhhhhhhh Abcam made a mistake
How to prepare a trypan solution 0.4% starting from powder 60% trypan blue ?
Wow, counting cell with inverter microscop. Please mention the type of hemocyometer ?
ruclips.net/video/GCohewzhD3k/видео.html, For the 2nd fomular, are you sure you want to use count1 to divide by 4, not count5 divided by 4?
I think is count5 divided by 4
For anybody stressing over the calculations at the end, these are wrong.
6.07/6:05: Formula= Desired number of cells to seed/Total number of cells=Volume to transfer/Volume of the cell suspension
10000/4000000=volume to transfer/20mL
10000/4000000*20=0.05mL which is equal to 50 microliter
Further
Total cells=4000000 and total volume is 20000ul so 1ul contains= 4000000/20000=200 which multiply by 50= 50*200=10000 cells