Biohacking the Oral Microbiome
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- Опубликовано: 16 июл 2022
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Papers referenced in the video:
Interconnections Between the Oral and Gut Microbiomes: Reversal of Microbial Dysbiosis and the Balance Between Systemic Health and Disease pubmed.ncbi.nlm.nih.gov/33652...
A Brief Introduction to Oral Diseases: Caries, Periodontal Disease, and Oral Cancer
tinyurl.com/z45d6djt
Xylitol, mutans streptococci, and dental plaque
pubmed.ncbi.nlm.nih.gov/19717...
Growth Inhibition of Streptococcus mutans with Low Xylitol Concentrations
pubmed.ncbi.nlm.nih.gov/18176...
The antimicrobial activity of essential oils and essential oil components towards oral bacteria
pubmed.ncbi.nlm.nih.gov/7478759/
Nitrate as a potential prebiotic for the oral microbiome
pubmed.ncbi.nlm.nih.gov/32732... 
Наука
Mouthwash recipe:
1000 mL water
10g xylitol
10g baking soda
2 drops peppermint oil
2g potassium nitrate
CORRECTED, see Michael's comments 👇
Also in other's comments here I see that sensitivity toothpaste contains high levels of potassium nitrate (but along with other chemicals), and some suggest that clean Listerine might also be beneficial for killing bacteria.
Almost right, Donna!
For 1000 mL of water, 10g each of xylitol and baking soda, 2 drops of peppermint oil, and 2g of potassium nitrate.
@@conqueragingordietrying1797 whoops! Thank you!
Nice summary! I might suggest pinning this comment for others' benefit.
Thank you
What toothpaste do you use?
What a great presentation!!
Interesting to know about the beneficial bacteria involved. I like reading about these bacteria. Even before the pandemic I’ve been rinsing and gargling with clove oil. So far so good. I would really like to keep all my teeth (and taste buds) till a ripe old age because I like eating^^
Super cool video and research!
Will be waiting for the results!
Excellent! Thanks Michael. Will be making some changes. And testing. Already eating a high nitrate diet and use higher pH water (~8) to make tea and coffee.
Wow! What a great video.
Beets look like a supperfood now :)
Great content. I have long-standing gum disease and it is controlled by
A) regular dental hygiene visits, 4 per year
B)using a waterpik machine
C) adding hydrogen peroxide to above
D) taking a prebiotic supplement
I will be encouraging my dentist and functional medicine doc to view this video.
Many thanks.
My dentist knows nothing about what's in the video, unfortunately. I asked them about optimizing my oral microbiome and got a blank stare.
What prebiotic are you using?
Quit eating grain, sugar, starch and high glycemic fruit at the very least until you can get the gum disease in check or you will loose your teeth. High glycemic diets promote oral disease and all the brushing, oral rinsing,dental cleanings will be a waste of time if you continue eating a high glycemic diet. The plant based diet people are completely wrong if you want healthy teeth eat mostly animal based food and low starch vegetables and you will heal your mouth. Dentists are largely a part of the problem.
@@rhyothemisprinceps1617crazy how people make these incomplete comments and never answer back
@@hardwiredtoselfdestruction3401 My favorites are Amazon reviews - "Didn't do anything" - "Works great!". What were they taking it for? What did it / didn't do? How much did they take? How long?
Can't wait dr Lustgarten.
I liked it already because I just know I will. Can’t wait.
I always get excited for videos, but I enjoyed the process even more for this one. Plus (imo), an interesting story!
Very interesting about pH buffering ability, thanks!
I've been brushing with Auromere toothpaste, it has many ayurvedic herbs traditionally used for oral health, many of which have effects on the oral microbiome (e.g. Neem). I wonder if your rinse could be improved further with additions from traditional pharmacopeia...
I drink teas throughout the day.☕ In the morning I do a chia type tea with cinnamon and cloves. Later I switch to peppermint tea. I used to add xylitol to the peppermint tea, but discovered that ingesting xylitol raises my blood glucose (contrary to common opinion, based on my CGM).
😊
Thanks!
Thanks Gokhan!
I'm making the mouth wash , thanks dude
Great video...very objective data. Ill be chewing on "beets" now throughout the day.
Thank you for another great contribution, and I really appreciate the siding of all the papers in the links, your logic behind the levels and how you approach the application of the knowledge to your own personal situation. It's a great example of how to apply with wisdom accurate knowledge. It would be interesting to see if environmental factors in the oral biome affect Downstream biome and then second order effects from that in terms of health and other processes in the body. I have read about biome transplants and that the younger biome being transplanted into an older patient can make that organism mimic a younger one, and it would be interesting to see if reducing our oral biome age, so that it resembled a Young Person's most idyllic oral biome, would that be a factor in reducing our biologic age?
Thanks David. Based on my oral biome and blood biomarker data, I don't think that the oral biome affects the blood biomarkers until it's too late (i.e. advanced gum disease/periodontitis). But, once oral health is bad, it may be too late to reverse it, so optimizing it in (relative) youth is now a key component of my approach.
I use a prebiotic, vitamin C, and D3, K2-MK7 and Co Q10 toothpaste and this keeps it up to a 5.5 ph.
Thank you very much for this great content.
I have been using so far a moothwash with sage tea, sodium bicarbonate, erythrit and salt brine, but I will definitely adjust it, thanks to you.
Do you know any study who showed similar effects like xylitol for erythrit?
And have you read anything positive about salt or might it have adverse effects?
Did you thought also about remineralization with silicium or other trace minerals?
I'm looking forward to an update.
Thanks Ni Cole. I haven’t studied erythritol yet-in terms of remineralization, calcium nitrate may be better, as calcium would be available.
The most important question that no one asks. Will Xylitol, sodium bicarbonate, clove oil, peppermint kill GOOD BACTERIA???? Will it kill everything. If it does, you do more damage then benefits.
Hey @nebojsa1976, the story has evolved a lot since this test, as I've tested 8x since. Here's the most recent video in the series:
ruclips.net/video/DkOceKds2a8/видео.html
Doctor, how do we eliminate the bacteria that cause chronic bad breath and replace them with beneficial bacteria?
I just watched you present this data at A4M - fascinating! thank-you. Do you use non fluoride toothpaste and do you measure NO production at all? (asking as Dr. Nathan Bryan says that NO made by mouth bacteria and mouthwash with alcohol and fluoride toothpaste inhibit). Thanks again - all the best - Sarah
Thanks Sarah! I use fluoride-based toothpaste, and haven't yet measures NO production. My homemade mouthwash doesn't contain alcohol-can you please post some links that fluoride inhibits nitrate metabolism?
my goal this year is to have more neisseria
Nitrates in food- Are they organic or inorganic salts like K & Na? Great original content.
Thanks D Dutton. I'm guessing inorganic salts, but I could be wrong.
I thought nitrite is converted into nitrate. Can you please explain. Love your channel
Hi. Maybe you cover it in another video (I've only seen this one)? You don't mention Vitamin D3/K2 (some say take 10,000 IU of D3 with 100 IU of K2 daily), generally for immunity and bones - which would further assist the Oral Microbiome. Just thought I'd mention it.
Thank you Michael. This is very interesting. You should turn your mouthwash into a commercial product and sell it.
Thanks Just Saying. I had that thought, too, but I think my strength is in guiding people to determine their own formula. Although that mouthwash may be best for me, it may not be optimal for others…
Could you link a source of the calcium/potassium nitrate if you find one? All the ones I see are for gardening purposes as fertilizer. And I'm not sure if they're safe to put in your mouth.
This is exactly what I bought (not sponsored!):
www.walmart.com/ip/Potassium-Nitrate-Powder-KNO3-1-2-Lb/730344998
Great topic and video. Do you shake your mouthwash solution each time before use? Do you floss, brush and use mouthwash immediately after each meal? Is potassium nitrate the only new addition after initial bristle test?
Thanks Sath Math. I do shake it each time before use!
The system is mouthwash (to remove any acids before brushing)-floss-brush-water rinse 3x, then mouthwash, spit, no rinse. Yep, potassium nitrate is the only addition (I'm at 4g/L, up from 2g/L in the video), but I've also doubled the peppermint oil (from 2-4 drops). Retest is coming soon, maybe another week.
@@conqueragingordietrying1797 Eager to see your progress and how you adjust. Thanks again for sharing your journey.
This is very informative. Based on this, I take it that you have data that Xylitol does not damage the "good" oral bacteria, that is, the bacterial involved in nitric oxide production.
Ah, the most recent part of this story is here:
ruclips.net/video/lMKycj_cp4U/видео.html
Bryan Johnson uses a tea tree oil mouthwash.
My oral care routine after meals is to rinse for two minutes with a concentrated green tea solution (1/2 tsp powdered sencha in 60ml 165f / 75c water), then chew xylitol gum that uses a natural chicle / non-synthetic gum base (Tree Hugger) for 20-30 minutes, and then brush with a toothpaste using xylitol (Bite). What are your thoughts on this routine?
A lot of xylitol studies use xylitol gum after meals where the chewing action / saliva circulation post meal may be just as important if not more so than the xylitol itself. Why do you think a xylitol mouthwash is a better option than a xylitol gum or toothpaste?
It'd be way cheaper and less hassle if I could just add 1g xylitol to the green tea solution and skip the gum, but I'm not convinced I'd get similar benefits.
I've seen that green tea may have oral benefits, but I'm hesitant to use it as a mouthwash because it can stain teeth. That said, I drink 20oz of green tea every day. Xylitol is a good add, but I'd recommend oral microbiome testing to see how your approach is performing. Everything else is just a guess.
could I get a brand name commercialy available with this type of focus . I am always settling on gum . its all poison , just use for saliva production
Really cool idea to add nitrate to your mouthwash. What do you think about using calcium nitrate instead of potassium nitrate so that you can get double benefit by having the calcium ions remineralize your enamel too?
Thanks John, and I hadn't thought about that! That's a good idea, I'll look to see if calcium nitrate is commercially available.
www.ncbi.nlm.nih.gov/pmc/articles/PMC5493431/
It seems like the potassium counter-ion on the nitrate might actually be actively bad? I would be interested in your opinion on this.
@@JohnSlack89 Hey John, why do you think that potassium in the mouthwash would be bad?
@@conqueragingordietrying1797 Hi, sorry, the article I just linked there: Potassium is a key signal in host-microbiome dysbiosis in periodontitis
Tipp: When you eat a ketogenic diet your oral health will be perfect in 2-3 Weeks without understanding this complicated stuff.
Any comment on why use potassium nitrate? Why not sodium nitrate for example?
Also where did you get potassium nitrate?
Sodium nitrate should work equally well-note that sodium is already in the mouthwash via sodium bicarbonate, so I went with the potassium salt to limit too much oral sodium.
Alternatively, calcium nitrate may work best, as you'd also have available calcium ions for remineralization.
I bought the KNO3 from walmart.
Where do you get potassium nitrate for this? Seems it's a controlled substance (saltpeter).
I got it from Walmart this time. It's hard to find, although not controlled in the US. It may be better to get calcium nitrate, as the calcium can potentially be used for tooth remineralization, but so far that's even tougher to find/may be less safe to use orally.
Wonderful !..................question: I have NAC induced arthritis............can you do some videos about the trouble you can get into with the RUclips Longevity Protocols !!!
Hey agaragar21, sorry to thear that, but the focus on this channel is diet+ exercise +blood testing as the primary longevity strategy, with a minimal role for supplements.
Never heard of this before. Could you please elaborate. I'm also taking NAC.
@@rajbista1478 I talk about that approach frequently on this channel. Search "Quantifying Biological Age diet".
What is NAC induced arthritis, how do you know you have it, how do you know what caused it?
How big of a batch do you make, how long does it last, and do you store it in a certain manner?
1 liter, it lasts about a week, stored in a regular water bottle
I did some crude experiments of my own to measure salivary nitrate and nitrite and learned that it matters how you collect the sample. I also read somewhere that bacteria have preference about where in the mouth they reside. I wonder if the test kit they send you instructs you how to swab or do you just spit in a vial? Didn't see any details on their website...
It's a saliva sample...
Seems like techniques for microbiome analysis have become more sophisticated. They don't seem to be looking under a microscope anymore but rather doing genetic analysis - so the saliva test might be fine in terms of detecting what is in between the papillae of the tongue. There's a gut microbiome test from Thorne that uses a rear end wipe to take the sample - much more convenient that collecting stool.
Hi there I'm just curious but what is the problem with conventional mouth wash? Isn't it also designed to reduce mouth bacteria and sometimes has the ingredients you mentioned?
Many conventional mouthwashes have additives to sit on the shelf, and are acidic, which is terrible for cavity risk. For example, see Table 3 in this paper: Listerine, for example, has a pH of 4.2:
pubmed.ncbi.nlm.nih.gov/24648842/
Use of OTC mouthwash is associated with increased risk of hypertension; it kills off nitrate reducing oral bacteria. There's a 2020 article on the topic by Joshipura et al., 'Over-the-counter mouthwash use, nitric oxide and hypertension risk'
Great video!
What percentage of potassium nitrate do you estimate you're adding? As a data point, commercial sensitivity toothpaste often has 5%.
Thanks Paul. It's 2/1000, so 0.2% Some toothpastes have 8% nitrate, but also other chemicals that I wouldn't want in my mouth hours after brushing. With the homemade mouthwash, I avoid that issue.
@@conqueragingordietrying1797 Do you supplement the mouthwash shortly after the final brush of the day?
@@rapamune In the morning, after brushing, mid-afternoon, and after brushing and flossing at night. So at least 3x/d, sometimes more depending on if I'm at home or not. No rinsing the mouthwash, just spitting it out, to potentially maximize any prebiotic effects.
@@conqueragingordietrying1797 Appreciate the protocol, looking forward to the update
@@rapamune Thanks Rama C. I'm thinking I'll send another salivary microbiome sample in about 2 weeks, and with a 3-week turnaround to get that data, we're about 6 weeks (1-week to make the video) from the update.
Thank you Michael, super interesting!
Sorry it's off topic, but I saw you ate 234g of mushrooms per day. Do you get side effects from that dose? Also mushrooms are a good source of lysine, with 0.1g/100g. So 234g has 0.234g, and I read the recommanded daily dose is 1-2g. I was wondering if it would be better to eat the mushrooms as powder in order to reduce the formation of MGL created by the cooking. And eat the powder throughout the day, not in one shot, as that might be too much for the liver and other things.
No side effects for me. Wait until you see my next blood test video-it's my best data of 2022, and that's with all the mushrooms. I boil them with barley or oats, so there's little MGL that will be formed. > 212F forms AGE products.
@@conqueragingordietrying1797 Ok I see, there shouldn't be much glycation.
If I am not mistaken 234g of mushroom provides 20mg of spermidine, plus you must get 15mg or more from the rest of your diet, so you must be at 35-40mg, that must generate a lot of autophagy! I think I'm gonna increase my mushrooms...
Looking forward to your next blood test video!
@@Battery-kf4vu Note that I've upped my mushroom intake in part because of spermidine and ergothioneine, but mushrooms are also a rich source of B3 and B5, which are significantly correlated with more biomarkers going in the right direction than wrong in my data.
@@conqueragingordietrying1797 Does the type of mushroom matter? Or do white button ones works?
@@jskweres2 I’m not sure if the type of mushroom matters, but as we’ll see in an upcoming video, that many mushrooms/day is a part of my approach for optimizing blood biomarkers.
Taking a sample of saliva does not have to be representative of the bacteria on the teeth, because they are contained in biofilm with it's own environment. Of course you know this but not every viewer knows this and it wasn't pointed out in the video.
Do we know whether any of your mouthwash ingredients can kill off "good" bacteria and potentially decrease NO production,e.g., the peppermint oil. I wonder to what degree, if any, natural bactericides decrease NO production similarly to commercial mouthwash. I have been a xylitol user for awhile now
Yes, that's a good point. What's toxic vs beneficial can be a fine line. The amounts that I'm currently using are conservative, nothing too crazy in terms of concentration.
I think this is an important video! So we agree that nitrates from you diet that end up in your the mouth are a very good thing? Nitrates in the saliva can be reduced to nitrites by bacteria such as Neisseria and Rothia and the when these nitrites are transferred to the stomach (when you swallow) nitric oxide is formed in the acidic environment and this is a VERY good thing. If I hear you correctly then, your mouth wash is effective at preventing pathogenic bacteria and allow necessary and beneficial bacteria to survive? I've been looking for just such a thing! Do you think that peppermint oil serves a purpose or is it for flavor? Thanks!!
Thanks @Mandolin1944. Yes on dietary nitrates for the oral microbiome. This story has evolved a lot since this video-I'd consider watching the other videos in the oral microbiome playlist, as the story (unfortunately) became more complicated...
I hope you have something to say about periodontal disease.
Only the bacteria that are linked with it..
Where are you getting your potassium nitrate?
I got 1/2 lb from Walmart
Hi Dr. Lustgarten,
Do you eat collard green raw?
Hi Richi, nope, cooked-I mix it with tomato, coconut butter, and 1g of salt.
@@conqueragingordietrying1797 Thank you for the reply and info.
Any thoughts on Xylitol wiping out beneficial bacteria?
Unfortunately, I can't assess that yet, because Serratia has been almost my entire microbiome for about half the tests...
See this most recent video for that data:
ruclips.net/video/DkOceKds2a8/видео.html&t=!s
Have you looked at how Nitric Oxide plays in the oral cavity?
Not yet-got any papers that I should check out?
@@conqueragingordietrying1797 Chemical generation of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate
C Duncan 1, H Dougall, P Johnston, S Green, R Brogan, C Leifert, L Smith, M Golden, N Benjamin
Affiliations expand
PMID: 7585121 DOI: 10.1038/nm0695-546
I take Human N Nitric Oxide powder that gives me optimal NO!
How do I restore my oral dysbiosis permanently?
Optimizing the oral microbiome, outside of a high nitrate diet (i.e. beets) is my toughest challenge to date.
Do you have any gut health test you would recommend?
I used Thryve last year, but I’m not sure that any guy microbiome tests are better than the others. In theory, VIOME could be, but it costs 4x more.
Look into nano-hydroxyapatite
yes, will do
I get migraines and avoid sodium nitrate in foods. I'm pretty clueless about chemistry but I assume that nitrates is fine for me considering I don't have a reaction beyond cured meats, but what's the difference between them?
How are your migranes when you eat lettuce, beets, or carrots?
@@conqueragingordietrying1797 Hmm, I don't eat beets, only have maybe a cup of lettuce max in a day (prefer spinach) And only eat cooked carrots. But no reactions from them!
@@SilverHawk214 Lettuce, spinach, and carrots have nitrates-if nitrate was the problem, I'd expect migranes after eating them, too...
You know toothpastes made for sensitive teeth have 5% potassium nitrate, I wonder if that is good enough? Are there no commercial mouthwashes you like? I know listerine has no alcohol mouthwashes with an essential oil blend that is suppose to kill pathogenic bacteria. I think I might add xylitol to it.
I've seen those toothpastes, but the difference is that I'm using the mouthwash as a prebiotic (no rinse after use), whereas I'm not sure if that's a good idea with the other chemicals that are found in conventional toothpaste (even the ones with nitrate).
@@conqueragingordietrying1797 Interesting. I've also had dentist tell me it's better to use mouthwash before brushing so that fluoride stays in contact with enamel longer.
@@MisterProducts For potential remineralization, that makes sense, but it from my experience, most dentists completely ignore or are unaware about how to optimize the oral microbiome.
@@conqueragingordietrying1797 I imagine it's a very new field of research without enough evidence to set medical standards are guidelines yet. Thank you for the tip about the xylitol, I will definitely use it. I'm assuming xylitol is the sugar-acohol with the most evidence for dental health compared to others like sorbitol and erythritol.
Still using this mouthwash recipe? :D
Hey @pyrgakis, off and on. The full story is in the oral microbiome playlist:
ruclips.net/video/EnkJqACCEE4/видео.html
@@conqueragingordietrying1797 Mercii