Immunopharmacology (Part-12) Monoclonal Antibodies (01) = Introduction and Production Techniques.
HTML-код
- Опубликовано: 23 авг 2024
- Participate in the FREE online test for this lecture- forms.gle/HZu6...
Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - play.google.co...
An antibody is a protein produced by the body's immune system in response to antigens, which are harmful substances. Antigens include bacteria, fungi, parasites, viruses, chemicals, and other substances the immune system identifies as foreign. Sometimes the body mistakenly identifies normal tissues as foreign and produces antibodies against the tissue. This is the underlying cause of autoimmune conditions such as rheumatoid arthritis and multiple sclerosis or MS.
Antibodies are naturally produced by the immune system. However, scientists can produce antibodies in the lab that mimic the action of the immune system. These man-made (synthetic) antibodies act against proteins that attack normal tissues in people with autoimmune disorders. Man-made antibodies are produced by introducing human genes that produce antibodies into mice or other suitable mammal. The mice than are vaccinated with the antigen that scientists want to produce antibodies against. This causes the immune cells of the mice to produce the desired human antibody. The term monoclonal antibody means that the man-made antibody is synthesized from cloned immune cells, and the identical monoclonal antibody produced binds to one type of antigen. Polyclonal antibodies are synthesized from different immune cells and the antibodies produced to bind to multiple antigens.
Steps -
(1) Immunization = Inject antigen in mouse
(2) Screen ing of antibody production and removal of spleen when antibody levels are high
(3) The fusion of myeloma cells with spleen cells using polyethene glycol to produce hybridoma cells
(4) Selection of hybridoma cells by culturing in HAT media
(5) Checking the hybridoma cells and subculturing them
(6) Cloning of hybridoma cell
Get in touch with the solution by just clicking following links-
Facebook Group- / solutionpharamcy
Facebook Page- / pharmavideo
New channel (Pharmacy Dictionary) - / @pharmacydictionary
Instagram- / solutionpharmacy
E-Mail for official and other work - solutionpharmacy@gmail.com
LinkedIn- / pushpendrakpatel
#solutionpharmacy #Pharmacologyclass #GPATonlinetest
This the best u tube channel ever ...!!no one can replace u sir hats off to u and your work💐💐💐
Thank you
Sir aap bahot padhate hai...mai aapke lec dekh ke hi gpat ki stydy kr rahi hu... aise hi achhe padhate rahiye;sir🎉🎉
Thank you so much for your kind feedback and support
Best teacher
My score 8/10,nice explanation sir
Badiya aadmi h👍👍👍👍👍👍
Thank you sir my score is 10/10
Good
Nice explanation sir
Thank u so much
I got 9/10
Thanku so much sir.....
Thank you so much sir❤❤❤❤
Best explanation sir.. Tqsm😊😊
Congratulations 🎉 sir
Our channel solutions pharmacy reached 500k friends
TQ soooooo much sir god bless you 🙏
10/10 thank you so much sir 🙏🙏🙏🙏
Very good
Thank you very much sir
Nice class sir
Sir 🙏
Great efforts ✌
Well explained
आप साक्क्षात भगवान का रूप है sir
Nahi nahi. Hum to sadharan se insaan hai sir
Sir please make video on how to report making after an internship..or please share report as pdf sir please we student need to discussion on that topic...
Very well explained.thnks
Thank you
Sir aap sach m bahut acha padhate or smjate h ,
Sir pls antibiotic padha digiye , or uske antibiotic ka use ,
Thank you. Already finished. Check
Solution- Pharmacy # sir ek bar agar repeat kr dete to bahut acha hota ..class of antibiotics, nd un antibiotics ka role kya kya h
Afa Ullah bhai video to uloaded hai to dobara kaise video banau. Aap pura seriese dekh lijiye
Solution- Pharmacy #ok sir☺️
Nice video Sir
Nice information sir ji 🙏🙏🙏🙏🙏
Thank you
Thank you sir my score is 8/10
Thank you sir... 👌👏👏👌
My pleasure
Excellent sir 🌹
Thank you
Very nice sir
Ramlakhan pal Pal thank you
Nice 👍
Thank you
10/10 scored🥰
Very good
ये क्लास d फार्मा की ही है क्या
one thing i didn't got. Sir ..myleloma cell kisey fuse hoga?? Spleen cell lekha huwa hey board par..spleen ka cell sey hoga ki yo b cell sey hoga??? susbcriber frm Nepal nepal
Spleen cell ko myloma cell se
Sir hum d.pharma me admission kara liye hai hum Aapke video se first se reading Karna Chahta hu .. lockdown ke ujah se college Nahi khul Raha hai .. aap samjhane Ka kripa kare sir ..
Abhi 1 year me aapke jyada kaam ke bus practicals hain is channel me
🙏🙏🙏🙏🙏🙏🙏
श्री मान जी
पशुओं में पेरगनैन्सी सेफ और अन सेफे दवा
को कैसे पता करें सर् किसी भी वीडियो में बता दे आप का आभारी रहूंगा।
Scored 10 out of 10..
Kya speed hai 25 minute ki video sirf 6 minute mai puri dekhli aur test bhi de diya
But that's not right way.
gaurav kumar yahi to maine inko kai baar reply kia. This is not a good practice. He should know this.
Sir , can u help me .
I am in final yr b.pharmacy, I want to know the scope and future in both economical and growth aspects ....
Pls guide me please ....humble request
Sir background me please play button ko laga lijiye please sir 🙏🙏
Thank you for nice idea. I will do that once get golden with your support
Sir to covid19 ki bhi vaccine monoclonal antibody se ban sakti hai h kya?
Sambhawna to hai baki exactly nahi kah sakta
Sir, 7th semester ka NDDS start kra do please 🙏 🙏🙏🙏
Hamari online classes start ho gyi hai but wha kuch samajh nhi aa raha hai.
Aap krao na please 🙏🙏🙏🙏
Sir jab koi bhi drug hamari body me enter kerti h as aantigen to hamara immune system activate hoker us drug ke against antibody kyu nhi banati ?
Antigen ke pass antigenic activity honi chahiye. Sab drugs unsafe nahi hote ek limit dose tak. Usse aage jane pe problem to hoti hai.
Sir I want a help pls sir
Ham mause ka use hi jyada kyo krte hai habridoma technology me