Indirect ELISA
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- Опубликовано: 25 апр 2010
- ( www.abnova.com ) - The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample. In the assay, the antigen of interested is immobilized by direct adsorption to the assay plate. Detection of the antigen can then be performed by using a matched set of primary antibody and conjugated secondary antibodies. More videos at Abnova www.abnova.com
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Holding the pipette vertical and pre-rinsing the tip will help you get more accurate results and reduce the variation between wells.
I like the way the video shows in details on how to carry out indirect ELISA test
this video with that song was much of a life changing journey as it was an educational video, haha thx for the video !
We don't have name for background music but we like it as well. Thanks for your support!
excuse-me but after coating can we incubate at room temperature for 3 hours or 4°C overnight for wichever proteins? Or this incubation is specific for protein that you're working in this video?
Thanks for video! It gives me more information about indirect ELISA tech.! Very useful!
@0102murphy No, the worst scenario would be cracking your plate if you're hitting extremely hard (which is unnecessary) but it is very unlikely for the Antigen/antibody bonds to break.
@MalagaJolu No, it really depends on the assay you are doing. For example it can be an antibody in Blocker Casein/PBS.
could u help me plz !!
we need to know the cytotoxicity of Macrophages and PBMC which are stimulated with LPS,PMA/PHA .. so we used MTT test and after to detect absorbance, we used enzyme linked immunosorbent assay(ELISA) plate reader at 570...
how we did this ELISA test !!??
What is the purpose of sealing the plate with parafilm? Thanks!
Will the coated antigen in the plate being shaked out if you hit the plate against the table so vigorously??
thank you
NEED HELP ASAP! WHICH ELISA TEST SHOULD I USED TO CHECK THE PREVELANCE OF JOHNES DISEASE IN CATTLE?? ILL BE USING BLOOD / SERUM SAMPLES. AND WHAT KIT # ALSO?
the coating buffer allways have to be 0.1 M Carbonate pH 9?
OK well, but Cual es la función de cada reactivo utilizado en ese experimento? : )
Why antigen needs to be diluted in coating buffer??
what is the name of the music?
nice one very advantageous
11 years ago ? dayum
thanks . 👍👍
I like . Good technic
thx u for viedio . tomorrow i must do elisa T_T for project . this viedio i understand >^
Nice
I like the video
Song is a banger a 2x speed
After coating antigen, why do you add the blocking buffer?
to prevent unspecified binding
why does the song go crazy at 1.75x speed
Interesting
By now Im able to know direct eliza
Ok, for example I have my purificated Ag ( I want to perform a indirect ELISA) in Tris buffer 50 Mm, 100Mm Kcl, do u recomend me hibrid my antigen to the microplates with that buffer?