Fluorescence Filters: Imaging Multiple Fluorophores & Dealing with Bleed-through

*This video walks you through best practices for imaging more than one fluorophore, including critical controls experiments, why bleed-through can occur when imaging multiple fluorophores and various strategies for reducing bleed-through. Enjoy!
* Visit FPbase.org/microscopes to generate a custom spectraviewer for your microscope.
* Visit Image.sc for expert help with calculating bleedthrough coefficient and image arithmetic.
* Visit Forum.microlist.org for expert help on the microscopy and sample preparation methods discussed in this Microcourse.
* Jost & Waters, 2019: doi.org/10.1083/jcb.201812109
* French subtitles were provided by BioImaging North America.

[@conehenge77]

Thank you so much!! That was very helpful.

[@woongyoungso4838]

Thanks for the awesome content! Looking forward to more!

[@jessebrunsveld1]

Great channel and explanation, thanks!

[@Walaa918]

Very helpful

[@hosseinghasemi1659]

awesome

[@rachanasomaiya8032]

Thank you!!!!

[@lotharmayring6063]

that is not the prolem of FM. The problem is that the images ar so unsharp. DIC is much better