Kurt Thorn (UCSF): Confocal Microscopy

GET UPDATED VERSION OF THIS TALK AT: www.ibiology.org/talks/confoc...
Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.

[@mrmatz408]

Extremely thorough and well-prepared discussion of a topic in which I had no previous understanding. Totally great!

[@nirmaljayadev]

That's just extra ordinary talent. To make such a complicated thing so simple

[@andrewareva4605]

If only this video was in higher resolution...

[@scicommlab]

+Andrew Areva We re-recorded this video a couple of years ago with higher resolution. Check it (and a lot of other microscopy videos) here: ruclips.net/video/1Q5V442V7xI/видео.html

[@brandond8488]

@@scicommlab Great use of super-resolution imaging 👀

[@ErichAmMeer]

try confocal

[@sylviahadai5291]

Thank you so much for this excellent presentation!!! ❤

[@rob66181]

Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks

[@xadroit]

Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.

[@mrunalsakharkar5463]

Excellent video. Very easy to follow. Thank you!

[@hectorjosecardona]

Excellent presentation, very didactic, thanks Kurt

[@priye19]

Thank you. Very precise and informative.

[@Nanomachines5on]

Good explanation of the diagrams. Thank you!

[@KeyManiacLad]

Excellent presentation!

[@MohammadAlKobaisi]

Very helpful, thanks!

[@sylviahadai5291]

Thank you so much!!

[@amarmirza08]

Thank you for making this video.

[@mindlessgreen]

Fantastic. Very well explained.

[@nishanthsurianarayanan296]

Thank you for the amazing explanation

[@maxencehannard1988]

Thanks for this wonderful video

[@scientia_potentia_est]

awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though.
Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512x512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe

[@djinnahwell]

Thanks, Thanks ! So great video.

[@aman0311tisf]

Excellent work Kurt!!! I've addad a link on my blog -

[@garrystewart3836]

Great help. Thanks!

[@painlessvoodoochild]

Thanks for great explanation

[@Maryam85ful]

Very nice video! thanks for uploading :)

[@caminhodeperfeicao273]

Thank you is very much for the video!

[@charme2377]

excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

[@MN-mj9ok]

Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?

[@dolet8]

explained very clear.

[@peterhartman55]

Great video.

[@sorrzietgiest]

very clear... helped me a lot... thanks..:)

[@paolofagherazzi1684]

Thank You very much

[@9haylee]

Excellent!

[@VSerman13GR]

Thank you!

[@leixiao169]

fantastic!!

[@djalitanaful]

I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?

[@antoniodimaggio4779]

this is such a good one! Thank you so much

[@BushCampingTools]

Confocal and multi-photon way to go! Spent years in research using, and later selling LOL!

[@ahmadnabawi5060]

a life saver !

[@psamrai7]

is there a difference between the dichromatic mirror and dichrioic mirror?

[@gregrobertson9732]

Nice one!

[@zhangcanding3263]

Great! Thanks

[@sasamaster]

Excelente! Muchas gracias, esto me ayudó mucho. :-)

[@uvesca]

yes... Awesome!

[@vman049]

Awesome!

[@xtremetom180]

very interesting thanks

[@xplorer9]

awesome!

[@megatog2011]

Very nice presentation there. However, if it was in HD (720p or better), I would have seen and maybe understood more ;)

[@tactileslut]

This sounds inspired by early innovations in the history of television.

[@FryChicken]

Confocal Microscopy starts at 3:50

[@FatemahRezayee]

Thanks a lot

[@anonviewerciv]

13:45 Apparatus.
18:28 Spinning disk.
22:30 Multi-photon.

[@sorrzietgiest]

wait.. working of spiral spinning disc is not very clear..

[@doktonic1370]

hell yes

[@edwindeleon1130]

need elctron microscope for hiv diagnoses

[@q0L0b]

LIKE.

[@sorrzietgiest]

spinning spiral mirror working is not very clear

[@kevinggyy]

like

[@humbertosmith6985]

Opino. Que. Diyegnnoñojia. Es. Maravillosa. Pero. Soy. Una. Simple. S🎃😊😅😂😂

[@lotharmayring6063]

unsharp unsharp unsharp unsharp unsharp ..................................

[@mmartin5816]

If only microscopists and histology people added scale bars. Don't show me an image without context!

[@venividiviolini6459]

"you can see that there is stuff that is clear and sharp and in focus.." yeah.. nah not really :'D

[@preynoldssco]

Why is this potato quality?

[@darlantro]

uploaded 5+ years ago. RUclips had harsher compression requirements back then.

[@darlantro]

also, probably an old camera

[@sylviahadai5291]

Thank you so much for this excellent presentation!!! ❤

[@sylviahadai5291]

Thank you so much!!

[@lizs7827]

Very helpful, thanks!

[@hitgucvkj]

Awesome!

[@redpinkgreenandblue]

very helpful, thank you :)

[@xplorer9]

Awesome!