Nice lecture. For the hard question. I assume at low pH the ester at the bottom will become protonated. If you move a pair of electrons from one of the sugar oxygens attached to the tricyclic rings at the top of the molecule. You can move them around (curly arrows) to release the carboxy group at the bottom to give you the extended conjugated structure which will now fluoresce. The freed up carboxyl group can then be used for further conjugation reactions. Very neat.
This is one of the lectures I found it very informative. Especially the HOMO and LUMO relation. Most lectures they just skip this fundamental principle. By the way, why not call HOMO highest occupied ELECTRON orbital instead of molecular? after all, it is the electron causing such phenomena. Thanks!
+Wulin Teo This is an old comment, but since no one responded, the reason is because the term comes from molecular orbital theory which makes distinction between atomic orbitals and molecular orbitals. Since these are molecules the behaviour of the molecular orbital is what they are working with.
Nice lecture. For the hard question. I assume at low pH the ester at the bottom will become protonated. If you move a pair of electrons from one of the sugar oxygens attached to the tricyclic rings at the top of the molecule. You can move them around (curly arrows) to release the carboxy group at the bottom to give you the extended conjugated structure which will now fluoresce. The freed up carboxyl group can then be used for further conjugation reactions. Very neat.
Wow! Real life Sheldon!
sir i need a little help in selecting probe for my research can u please help me by ur kind suggestion
Amazing video, totally need this for flow cytometry!
This is one of the lectures I found it very informative. Especially the HOMO and LUMO relation. Most lectures they just skip this fundamental principle.
By the way, why not call HOMO highest occupied ELECTRON orbital instead of molecular? after all, it is the electron causing such phenomena.
Thanks!
+Wulin Teo This is an old comment, but since no one responded, the reason is because the term comes from molecular orbital theory which makes distinction between atomic orbitals and molecular orbitals. Since these are molecules the behaviour of the molecular orbital is what they are working with.
+bv Thanks!
Thanks a lot sir...
Wonderful video
Which probes can be used for targeting of lysosomes
Are Fluorescence labels and Fluorescence probes are same ??
What software can be used to create a video like this?
pecatum666 adobe after effects with a screencapture software and a greenscreen as a background.
GREAT!!!