How to Grow Mushrooms: Using Liquid Culture to Make Grain Spawn
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- Опубликовано: 16 июл 2024
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Quick tip: You don’t need the needle on top if you’re spraying into the top!
why not use the port because that is what it is for
Small advice: new seringes are sterile. No need to heat them before innoculating
I'm so happy I found your channel. I had been talking about growing mushrooms but never tried it. In the summer my grandma gave me a pink oyster kit just to try out how fun it was. My 6 year old son and I loved it so much that I have been trying to learn how to grow my own. I wish I had watched your cloning videos before we ate them all lol. Thank you so much for all you share about this amazing hobby (for me anyway). We really love watching. I've learned more from your videos than the many hours I spent reading prior to finding you. Thank you so much!
Watching is 10x faster than reading.
Ive had a few successes my first tries, then a few failures. Brf cakes i ruined by using contaminated water in a self made spore syringe.
It’s pretty easy too👇🏻🫡🇺🇸🇺🇸🇺🇸
Great Advice!
Usually when people tells you, your wrong, then you know your on something Big”
Yep! We agree!
Thanks for explaining what you did with the rice and showing us how to handle/transfer the final product.
I really like your thorough use of spray alcohol: can never be too careful. Thanks for the video.
Thanks!
I like to inoc 1 to 2 qt jars then multiply them into larger grain bags. With all the strong mycelium points they colonize quite fast for me. I have a lot to learn myself. Next for me is single strain agar to grain. Cant wait!!
Would love to see you review your old videos live and provide a bit of commentary on what you do differently
You're a good teacher. Thank you.
Thank you for that!
Lol, I just found out about grow kits and I’m already obsessed. I love the no-nonsense hobby versus business sides of your how to videos and also your failures are even more important because that way those of us who have never done it won’t be discouraged if our first few trying out runs have issues. We know to expect mistakes and what may solve them. So thank you.
Hey can u tell me the difference between buying what he said and this method I don’t really understand this small part of the process.
I've been watching so many videos, re: mycology... This is the first one, I've watched by you.... You are the man... Thanks...
Thank you for watching!
Great video ... really like that you showed yourself going crazy with alcohol. I had forgotten if that could harm the culture and i take this video as a *Safer is better than a little culture* ..... you are officially my 1st bell i will follow.
Excellent tutorial. Thank You!
Thank you!
Humble guy.. gentleman and a scholar.. looking forward to more.. new sub
Very happy to see a professional share this priceless information. Very much appreciated.
You Sir are a Boss. Nice thanks for the Gr8 video and the MOSSY! Cheers friend!
Thank you, and right back at you.
REALLY appreciate you sharing your knowledge! Spawing away here...
Awesome, engaging video
NICE! MOSSy. Got your back in WI.
Woooah, I didn't expect to see that the m\Mycelium Emporium hails from my home state. That's pretty cool
youre awesome dude :D thanks
Thank you quite helpful.
You are very welcome, thank you for watching!
Thank you so much for sharing your knowledge. Could I suggest a How to store video or even some information in comment? How, where and for how long to spore prints, spore syringes,spawn agar dishes and seed bags etc. Thank you.
Thank you 😊
You remind me a lot of a very good friend of mine, definitely subscribing
Andrew - great videos! I met you at a Thai restaurant on your birthday trip last year. Your wife is great in so many ways.
Thor is awesome. I wish I could find pelletized soy hulls.
In your cleaning video it appeared you were wiping with bleach - and no gloves.
Bleach is much worse on my hands than isopropyl.
Keep up with the great sharing. I will be trying the pathfinder oyster.
Mossy Creek Mushrooms
It was the Thai restaurant in Telluride
I was the guy dressed like Paul Stamets.
I am so glad you are becoming a Rockstar RUclipsr.
Next time I go to the Telluride mushroom parade I can go as you.
That is such a relief - I don’t need to lose 80 lbs to pull off a realistic TR Davis costume :)
@@Mossycreekmushroomsofficial oh my god a video on thor ?
iam SO excited for that one
litteraly just posted a comment with a request for more detail over your bagger (thor) and now i read this ! :D
Thank you.
Where do you get your spawn and fruiting bags? Awesome video, I'm a newbie and trying to wrap my head around mushroom growing
I am very happy to have discovered you and enjoy the informative videos! Do you have a coupon code for your own website? Thanks
Do you have a video on how you load your bags in the PC, and your process.
I'm just getting in to bags, and I'm looking to do it the new way (no tyvek, or popped bags).
Thanks MC
Check out our grain spawn video, that shows how I do a lot of it. Thank you!
Super helpful. What is the brand or type of bag you use for your grain spawn here? Thanks!
Great video. You said you don’t need to use the whole syringe but what do you consider to be a sufficient ratio of millilitres of liquid culture to grams of hydrated grain?
You might be able to look into some aeroponics nozzels and just have them spray alcohol over the operation for you so you don't have to keep grabbing the bottle.
Once you have used the entire syringe of liquid culture can you draw 5cc of unpopulated liquid cutler to see if there is a few mycelium are still hanging out in the syringe? Maybe they will regenerate from the few that are in the syringe?
I’m kinda curious. What do you do with the substrate after you’re done growing your mushrooms? Does it go to a nursery as mushroom compost? Do you dump it somewhere? I couldn’t imagine you’d have the space to compost it yourself since you work out of your home in a neighborhood. (?) Not sure what your living situation is.
Great. Can you put a tear of the liquid culture in a petri dish, to keep it in your mushroom library ?
What temperature do you keep your bags at after injecting spores?
Hi. I just came across this video. Thanks for taking the time to make it! I want to try growing my own mushrooms and had previously come across the liquid cultures which is what led me to this video. Just a quick question... You flamed the needle instead of using the alcohol spray. Is the spray not enough to sterilize the needle or is flaming it because you don't want alcohol to contaminate the culture as you're injecting it into the grain?
Is there any other hygienic way around the hepa filter besides the hygienic port you mentioned...for the culture to substrate transfer?
Great Video! Where do you buy your grain from? Is it better to buy local ya think? Great video, appreciate your expertise!
Are those bags you use microperforated? when the seal is without air intake?
I'd love to get your input
I usually drop agar but when I do use a liquid I make a LI with agar
Basically:
Drop agar into sterilized water with a ball of foil and just shake the crap out of it
I can usually make 50-100cc of inoculate with it
Just something fun that I'd love to have your input on
Hey mossy greek mushrooms, thanx for the vid, Is that brown rice? And should it be cooked or not, before we inoculate them... Thanx
Hello, im glad i can find some info about this, can you tell me the proccedure for the grain, i mean, what proccedure they gone tru when/how you put them in the bag and in the pot, etc.
Thank you so much !
Pasteurise dung or sterilise substrate. Search those things on yt
I caught my still air box on fire today. I'm just glad I didn't have more fuel out lol
Was it bad? Did it burn you to shit? I'm always worried about that. I did an experiment to see if it would explode and it did and burnt my facial hair off.
@@bradpotetz9844 Use 70% and it will be less of a problem. You can get burnt, I didn't, I got lucky
@@PG1000. dats what I did!!! It was lowkey awesome tho, listen. I had my LC jars and needles and shiet in a box , sprayed alcohol wiped it down waited a minute, sparked my lighter to sterilize the needle 🤣 and it made a weird big flame , and I let the lighter go so that it would go off, AANNNDDDD the flame stayed lit in the center of the box and kinda flickered like 2-3 times, (paralyzed in awe) watching the swirling turning a little more bright yellow and then poof the entire box spiraled into a fireball and I dropped my red hot needle and ruined one of the bags and burned the hairs on my arm trying to pick up the needle while it’s swirling on fire 🤣
what is the shelf life for grain spawn?
is there hole or any air medium for inner bag is needed for the grain spawn
Helll yeah.
Since you did not inject into the bag do you really need a needle if you are opening the bag and squirting the liquid culture in?
Sorry for lame question, where you wrote the strain is that tape to let it breath? Or are the bags completely sealed
Thank you for another great video! I have to ask, what's that new mushroom I see you wearing? I can't tell if it's just an ornament, a whistle or a pipe? Either way, I'm sure it would do well as merch looks good, I'd buy one.
how long do you leave the bags in the pressure canner
Is this the same steps to grow mushrooms from a syringe? Im just looking to grow some myself and I'm celiac so can I use wood chips?
This bags that your using...where can I get those? Assuming that lil patch on them allows it to breathe?
So i am New to all of this. I am trying to learn all the different methods. For stating good tek. Using premade up ones while i take in everything and learn good starting places. What you said in one of your video is people holding back information. Which is hard to understand myself. When regardless of making your own spawns or liquids or ajars your always going to have to reach out to one another at some time for some kind of product to help you get your product to were you want it to be
Im just looking for good guides so. What up with the sugar to sugar transfers can you go into some detail with that for me
Great videos. Thank you.
Where do you get grain from?
Thank you, I get it from the co-op.
Hi! Besides Rye rice what other sustrate can I use to innoculate the liquid culture? Thanks!
What size bag do you use? Where do you buy them? Can you give me the bag specifications? Thanks!
Excellent thank you...what is the temperature of your spawn bag grow room please and is it constant?
26.5c & yes
Is spawn necessary to have? Like could one just use liquid culture on cardboard to grow blue oyster mushrooms?
I’m just starting my mushroom journey. I ordered 7 different liquid cultures & inoculated Uncle Bens ready brown rice bags. The mycelium is growing so fast! I’ll use those original bags to inoculate several other bags before putting them into my substrate. I’m glad I’ve got some time because I don’t have everything set up yet. Just trying to have what I need before I need it, but not before. I’m really excited!
I'm new to this and using Uncle Ben's
Did you make holes at the top for gas exchange, and if so, how?
Everything was going fine with mine, and then they crashed and started to have this empty strange smell.
I don't think my GE holes were big enough, or I used the wrong micropore tape to seal them.
Hope you've had some luck!! :)
@@RealBradMiller I’ve had a lot of contamination not having a clean room, but I’ll persist.
I cut the top corner off & used micropore tape to cover it. Make sure you’re using paper tape.
Sounds like me right now . 😅How’s it going? Been 2 years.🤞🏻
@@remingtonfrog3170 It didn’t work out so well for me. Mold was a huge issue, so after spending hundreds of dollars, I gave up 😟
What temperatures do you have the grain bags in?
You should just have a pool of isopropyl that you dip everything in lol. You go crazy with spraying that
I want to grow some portobellos. Any advice?
I hope you are still able to read all these comments, because I could use some help! At 12:40, you are discussing why you don’t prefer using LC commercially, and mention the high contam rate once the GS hits the high nutrients in the master’s mix. This has been my experience consistently after dozens of attempts, with a variety of manure loving LCs (I’ve had better luck with hard wood lovers.) I was only pressure sterilizing for 90 minutes, though, and wonder if that could be why? I’ve pressure canned food for decades and have never gone over 90 minutes at 15 psi, so I figured that was good, but maybe not? Or should I try a less nutrient rich substrate mix, perhaps? Thoughts much appreciated!
(I binge watch your content periodically, I’ve learned a lot from you! Thank you for sharing your knowledge!)
what are you spraying with? alcohol or 70 % peroxide?
How many quarts is that pressure cooker?
Can I inject the liquid culture directly into the substrate and bypass the grain spawn procedure??
water and honey work if you dont have grain?
do i have to prep store bought grain bags?
Whats the spray bottle mix? Is it 4tspns per qt of bleach to water?
Please can you do (or have you ever done) a video on recognizing failures? What does contamination in a bag look like? What does a failure to seal the bag look like? In one video you talked about the mix being too hot and it killed the growth, and the bag looked dark inside. What was going on there?
Great ideas~ Thank you! Added to the list!
@@Mossycreekmushroomsofficial I've been totally amazed by your videos. Finally assured that I've seen all 51 of them. I'm in New Zealand so I can't become a customer sorry. You guys have been an inspiration for me. Thank you so much.
Hi , why you cook your substrate ? do you not think that will be so easy when you sterilize using the Hydrated lim ?
Can you use 151 grain alcohol
you prefer agar to grain in general? I'm a total noob and it seems like most people prefer LC for speed.
May i ask if we can used Grain spawn transfer to Liquid culture ?
How much water do you put in your 23qt presto with 4 bags in it?
How do your bags not melt in the pc when they touch the sides?
I have a serious question. I just accidently injected 2 different liquid spawn into a grain jar. Is this bad news? What's the end result/issues? Not 2 different jars. I accidently put 1 cc of 2 different into 1 jar
That’s easier,quicker,safer and efficient way , 👍
U can cut life circle of contaminating bacteria very easy
Where do you get the stuff for the master mix? All im finding is really expensive stuff that makes it not even worth it for me..
Find your nearest farmer and make a deal to clean up spillage.
bro can i germinates spores directly in liquid culture?
Which plastic bags did you use? Can regular plastic bags tolerate the temperature and pressure from the sterelisation?
Great video!
Best regards
You'll want to get the pre-sterilized grain bags if you're beginning.
What kind of grain is that?
Hey man, sorry for asking random question.
I recently bought mushroom spawn that are growing in barley grain.
I am wondering whether can I transfer and grow the mushroom spawn into liquid culture containing glucose and yeast?
Normally, people will grow it in liquid culture and transfer it into the grain.
I am just wondering if it is workable if do it in otherway round.
You could do that, but it would be easier to take colonized grain and put it on Agar first, then innoculate the LC with the agar.
can i use cane sugar instead of honey?
Jaggery be used instead of honey?
Could you please explain solid to solid transfer. A question from a newbie. Thank you.
Agar to Agar. Meaning that I would cut a piece of agar from a petri dish, and use that to inoculate a new plate.
@@Mossycreekmushroomsofficial its super easy to make an agar dish from liquid culture though.
What is solid to solid transfer?
Brother mushroom spawn packet is available in Qatar market
Instead of grain 🌾 can I shoot lequid culture directly into the substrate.
Has anyone tried out?
Awesome! What grain are you using? Unrelated question, do you have a cooler to store product until delivery day? We were out knocking on kitchens yesterday, picked up 3 new restaurants, woohoo.
could it be oats? it would be nice to know some millet spawn preparation tips, I like to use millet for master inoculated by LC and then g2g to rye. I soak millet for 6h and then sterilize, but colonization of millet master jar is very slow compared to rye :(
@@Mossycreekmushroomsofficial Just read your blog, awesome!
Good video man I still say that Paul Stamets fermented mycelium culture technique is the most effect transfer tek I have ever studied. True liquid culture manufacturing requires expensive lab equipment that most of us can’t afford. As long as you develop a good scientific method for sterilization has down grain to grain transfers are the pinnacle of mushroom growing for any style of grow
I like your Mushroom Pendant (and your Videos :D)
bagus mana untuk penyubur panen 2;3
needs more alcohol!!
Always.
If contamination is a big concern there are also sterile sleeves you can wear.
Makes sense.
I want to start growing, I want to use straw and the bags. Where should I get my spores to start out
@@Mossycreekmushroomsofficial can I have your website, i really like how you started out. I'm wanting to make it a full time business. Starting off small. I have a fruiting room all setup and rdy.
So is the whole point of not using liquid culture for production block that if there is a contamination, you just inoculated a bunch of blocks with a contamination?
Out of curiosity, why do you put a needle on it? Can you not just squirt it from the syringe directly?
Contaminants. Unless you're in a clean room (and even then, because even in clean rooms shit happens), good lab procedure is to sterilize the syringe between bags, jars, etc. There's spores and bacteria floating around everywhere, and using it without a metal syringe makes it harder to sterilize (you could spray the nozzle with alcohol, but then you're risking getting some alcohol in the culture, which is no good either.)
It's better to spend a minute now practicing good lab procedures than risking the hours you spent working on spawn that goes bad.
It seems like if you’re not using the needle adding a needle only adds potential contaminants?
Go through extensive lab procedures, only to not wear a face mask and talk to an audience on camera. Haha. I love this guy though he's awesome, but the mouth is probably the dirtiest part of a person besides the colon and anus. Lots of fungi and bacteria in the mouth. Also ppl are so afraid of their cultures ruining. On grain it makes sense cause that's more ideal for bacteria and mold then mushrooms. I'm fruiting oyster mushrooms in a bag I didn't sterile, on cardboard I didn't sterilize, cloned from stem buds I obtained none sterile. Also had it culture in a none sterile room with holes poked in it none sterile needle and no filter patch. Some mushrooms are tougher than we give them credit. We treat them like they're biologically weak, but think of the places they grow in nature. They're not weak, they just need a good head start and the right growing materials and conditions. We make them weak by constantly sheltering them from life.
Germ x advanced has isopropyl alcohol, not ethanol. More than likely most hand sanitizer are going to have isopropyl or n -propanol rather than ethanol. All to say, there is no point in avoiding using isopropyl as a skin disinfectant, hand sanitizer is the same thing, especially germ x brand. Probably be better off getting 5 gallons from somewhere that does wholesale deals. I get a gallon of 99% for 12$ and cut it to 70%, and that last a while. Ps don't grow mushrooms yet currently produce tissue cultures of plants and animals , but do have lots of lab experience about to go to grad school for genetics. Starting to get very interested in mycology. Not sure how well-advanced genetics fits in to this field I've read a lot of studies on mushroom genetics but they seem to lack the technical difficulties I require to stay interested. Might just be a hobby to do some home genetic modifications or somatic hybridization.