I'm an aspiring hobby mycologist as of now but I'm working on studying and learning more. I'm excited because I'm getting my first laminar flow hood this week. It's only a 12" x 12" but I'm happy to be able to have it in order to do agar work. I've been watching your videos trying to learn as much as I can because I truly believe you have the most intelligent and informative videos pertaining to mycology. Mush love.
enjoy man. make sure to sterilize everything thoroughly I just built a 2x4 flow hood and its kick ass finally got fully contaminant free transfers and pours
Thank you brother for the inspiring and helpful videos that you share,those educational videos about mushroom growing are very helpful and inspiring.God bless you
Long ago, before all surgical rooms were made as pos pressure rooms, only the designated orthopedic suites had +laminar flow. I worked with travel nurse companies for many years and if the shift was all travelers and we weren’t absolutely sure which suite had laminar, we just went room to room pushing the door open… when we got blasted backwards by the air, “Found it guys!” Picturing it as water is the best way to explain it! (The reason that once we started a total joint surgery, no one was allowed to go in and out of the room until the dressing was on. Mess with the room air ebb & flow… and the surgeon will make you wish you had never been born! 😂)
Agreed. +1 to the advice of having negative pressure in your grow room. If you have positive pressure spores will be pushed out and get everywhere, inside vents, filters, etc. Negative pressure will suck the air into the grow room and it will be properly exhausted where you want all the spores and co2 to go. Thanks for the video Gary. What a beautiful sunrise you have!
Dude don't take this the wrong way, but I love you man. I got my first grow kits 4 days ago and I've never been this excited about anything in my life. Mycology is my calling and this is my future. Just want you to know that I have learned a lot from you already. Sorry I didn't leave likes like I should have on all your vids, but I hope to make that up to you. I want in dude. I am interested in establishing a relationship with you as soon as possible. You are without a doubt one of the best sources I have found. Thank you for everything you are doing to grow this community. Please tell me the best way to keep in touch with you. I need help getting started. As a disabled person I have limited resources, but want to contribute any way I can. ps I chose Lion's mane and Red Reishi to start with, but hope to expand into the oysters and then next year MORELS. PSS I am in Iowa. We have a morel farm here in Vinton. I want to compete.
Gary: "I'm sure you are familiar with Archimedes principle" Me."ahh...sure, which one did you have in mind? He had so many". :) Great stuff as always....thanks for all your contributions to this community. Not many people can go to sleep at night knowing they are helping make the world a little happier, healthier, and more connected....you are.
I would like to note that in a BSL 3 lab there is a designated biosafety cabinet and air lock to prevent the spread of pathogenic organisms, but the overall pressure of the lab is positive pressure 👍🙂
Negative. If you have positive pressure then any unseen gap or hole or open door( even to an air lock) is a potential escape route for a potentially pathogenic organism. Negative protects the worker from the culture, positive protects the culture from the worker.
@@ghz24 Positive pressure in the room to protect the material being used from contaminants - negative pressure in the biosafety cabinet to protect the technician - air locks into the lab to stop any potential air leaks and negative pressure overall lab being filtered to contain any contaminants that may escape the workspace. It is more complex than just one or the other, but for a mushroom farm there should be positive pressure in the lab.
Morning Gary! I really like how you break things down in a science minded way. So I asked about creating positive pressure the other day after watching your video on adding a HEPA filter on the door in your lab. [After watching your video, I ordered an 8" filter to accommodate an 8" VIVOSUN inline fan I bought for another project] I have two different exhaust fans in my lab, much like you would have in a bathroom. They pulll the air up and out through the ceiling.[not real powerful, but do pull some out. Would you suggest that these be on when I am in the lab, while the flow hood is running and the HEPA filter I am installing today [pushing positive pressure in] is running? Thanks for all your help! Rob
it depends on the flow rate of those fans, it could be creating negative pressure and suck air in from under the door, I would just make sure the intake air is more powerful than the exhaust to create a positive pressure, otherwise turn them off
thank you a lot this was baducly the one video actually describing what i need to do with all the ventilation i was really stuck up on it for a little bit 😂
Thank you! I'm new to mushrooms, with the help of a friend who doesn't grow the same species, and the internet. I'm building my first set up, and I'm a measure three times cut once person. At least now I am; I'm enthralled. This video cleared a lot up for me, because I've read some new growers' experiences and they had positive pressure in their grow rooms. While researching ventilation, someone much more experienced mentioned spore lung, so in looking up positive vs negative, I was lead here. Well, the short version of this story is a question, do you filter the air that gets sucked into your negative pressure grow room? My gut says yes. My brain asks how. I'm planning on using a grow tent for the time being. I hope you can answer! Thank you again, for doing what you do.
yes I like using the purple automotive hepa filters going into the resevoir and it’s important to exhaust the grow tent outside. With that setup you won’t have issues because the grow space will be completely separated by the tent and contained air
ill be grabbing some of those Blue Oysters from you for sure. I bought some Pink Oyster from you off Etsy a couple months ago, they are fruiting now and they are absolutely GORGEOUS! my wife will be making a dish out of them for Thanksgiving dinner, I can't wait! Fast growers and very stable. your QC is top f'n notch. thanks brother! Im looking forward to seeing your lab when its done, you really know your stuff, and thank you for sharing your knowledge with us!
Electrical outlets are often overlooked as sources of dirty air sneaking into a space. Sealing them with Silicone is quite effective. Take the faceplate off, remove the outlet from the box and seal all air holes in the box and around it. Then reinstall the outlet and faceplate.
I have a question that maybe you can answer. Can mycelium overpower contamination or even eat the contamination? I've seen heated debates on this but I'd like to know your opinion as you're clearly educated in the field. I have had jars of mycelium show signs of contamination in which I quarantined them and it looked like the mycelium won in the long run so I went ahead and fruited them successfully for 2-3 flushes without contamination appearing. I've read that once bacteria sporulates within the mycelium it's there to stay but I'm just wondering how safe it is to actually fruit mycelium that this has happened to. I saw someone doing a study where they were trying to train mycelium to have greater resistance to contamination by allowing the contamination and mycelium go on agar until the mycelium took over and then transferring and then fruiting a generation and then running the same experiment again with hopes of building up a resistance. Is this futile or does it have merit? If I was closer to where you are located I would 100% attend your classes that you do but unfortunately I'm not. Thanks for taking the time to answer (if you do). Mush love.
I will need to research more to have a clear answer on this - there are exudates that mitigate contamination (i.e. antibiotics) but Im not sure if the mycelium eats the bacteria or not - there are mushrooms that consume worms in soil (nematodes eaten by king stropharia) but they are larger in size than bacteria. I would not use contaminated mushrooms of any kind because there is the potential for getting sick or even consuming mycotoxins from pathogenic molds etc. my philosophy is “when in doubt throw it out” hope that helps!
@@FreshfromtheFarmFungi Did you ghost my reply where I disagreed with you about the BSL-3 lab being negative not positive. pressure that you stated? i am sorry you got confused on this point but you are wrong. Positive pressure would cause a possible escape route for the potentially infectious agent at every leak or crack in the walls/enclosure. Instead negative pressure assures that all the air escaping the room goes through the HEPA filter and gets trapped before it can escape. Please look it up.
@@ghz24 You are wrong, there is a tool called a manometer that we used every day to check pressure for our clean rooms in the last BSL-3 Lab that I worked at. Every room was positive pressure, but the overall "core" of the lab was filtered and had air locks to prevent any air from escaping. It is more specific than I stated in the video, yes, but all rooms were positive pressure to maintain the sterility and cleaned thoroughly after each procedure and tested regularly to maintain the status of BSL-3.
Dommage qu'il n'ait pas laissé le père répondre à la question "vous n'avez pas peur que votre fille vous reproche un jour ce choix?" Comment un enfant de 8-9 ans peut-il savoir ce qu'il va faire dans 10-15 ans ? Est-ce que ce choix ne limite pas les possibilités de choix pour ces enfants quand ils seront plus grands?
I'm an aspiring hobby mycologist as of now but I'm working on studying and learning more. I'm excited because I'm getting my first laminar flow hood this week. It's only a 12" x 12" but I'm happy to be able to have it in order to do agar work. I've been watching your videos trying to learn as much as I can because I truly believe you have the most intelligent and informative videos pertaining to mycology.
Mush love.
enjoy man. make sure to sterilize everything thoroughly I just built a 2x4 flow hood and its kick ass finally got fully contaminant free transfers and pours
Thank you brother for the inspiring and helpful videos that you share,those educational videos about mushroom growing are very helpful and inspiring.God bless you
You are killing it with all of the videos. I appreciate how detailed and concise you are. Keep it up man! Can't wait to see what's next.
🍄❤️
Long ago, before all surgical rooms were made as pos pressure rooms, only the designated orthopedic suites had +laminar flow.
I worked with travel nurse companies for many years and if the shift was all travelers and we weren’t absolutely sure which suite had laminar, we just went room to room pushing the door open… when we got blasted backwards by the air, “Found it guys!”
Picturing it as water is the best way to explain it! (The reason that once we started a total joint surgery, no one was allowed to go in and out of the room until the dressing was on. Mess with the room air ebb & flow… and the surgeon will make you wish you had never been born! 😂)
Agreed. +1 to the advice of having negative pressure in your grow room. If you have positive pressure spores will be pushed out and get everywhere, inside vents, filters, etc. Negative pressure will suck the air into the grow room and it will be properly exhausted where you want all the spores and co2 to go. Thanks for the video Gary. What a beautiful sunrise you have!
Dude don't take this the wrong way, but I love you man. I got my first grow kits 4 days ago and I've never been this excited about anything in my life. Mycology is my calling and this is my future. Just want you to know that I have learned a lot from you already. Sorry I didn't leave likes like I should have on all your vids, but I hope to make that up to you. I want in dude.
I am interested in establishing a relationship with you as soon as possible. You are without a doubt one of the best sources I have found. Thank you for everything you are doing to grow this community.
Please tell me the best way to keep in touch with you. I need help getting started. As a disabled person I have limited resources, but want to contribute any way I can.
ps I chose Lion's mane and Red Reishi to start with, but hope to expand into the oysters and then next year MORELS.
PSS I am in Iowa. We have a morel farm here in Vinton. I want to compete.
You're truly an inspiration and I find your videos absolutely fascinating and of course, always informative.
Gary: "I'm sure you are familiar with Archimedes principle" Me."ahh...sure, which one did you have in mind? He had so many". :) Great stuff as always....thanks for all your contributions to this community. Not many people can go to sleep at night knowing they are helping make the world a little happier, healthier, and more connected....you are.
Super sunrise action with gary positively pushing that tam from the mycosphere- brilliant-thanks
Excellent explanation, Gary. Yes, please, more like this. Thank you. 😊
I would like to note that in a BSL 3 lab there is a designated biosafety cabinet and air lock to prevent the spread of pathogenic organisms, but the overall pressure of the lab is positive pressure 👍🙂
Negative.
If you have positive pressure then any unseen gap or hole or open door( even to an air lock) is a potential escape route for a potentially pathogenic organism.
Negative protects the worker from the culture, positive protects the culture from the worker.
@@ghz24 Positive pressure in the room to protect the material being used from contaminants - negative pressure in the biosafety cabinet to protect the technician - air locks into the lab to stop any potential air leaks and negative pressure overall lab being filtered to contain any contaminants that may escape the workspace. It is more complex than just one or the other, but for a mushroom farm there should be positive pressure in the lab.
Morning Gary! I really like how you break things down in a science minded way. So I asked about creating positive pressure the other day after watching your video on adding a HEPA filter on the door in your lab. [After watching your video, I ordered an 8" filter to accommodate an 8" VIVOSUN inline fan I bought for another project] I have two different exhaust fans in my lab, much like you would have in a bathroom. They pulll the air up and out through the ceiling.[not real powerful, but do pull some out. Would you suggest that these be on when I am in the lab, while the flow hood is running and the HEPA filter I am installing today [pushing positive pressure in] is running? Thanks for all your help! Rob
it depends on the flow rate of those fans, it could be creating negative pressure and suck air in from under the door, I would just make sure the intake air is more powerful than the exhaust to create a positive pressure, otherwise turn them off
Thank you for this simplistic explanation.
thank you a lot this was baducly the one video actually describing what i need to do with all the ventilation i was really stuck up on it for a little bit 😂
Thank you! I'm new to mushrooms, with the help of a friend who doesn't grow the same species, and the internet. I'm building my first set up, and I'm a measure three times cut once person. At least now I am; I'm enthralled. This video cleared a lot up for me, because I've read some new growers' experiences and they had positive pressure in their grow rooms. While researching ventilation, someone much more experienced mentioned spore lung, so in looking up positive vs negative, I was lead here. Well, the short version of this story is a question, do you filter the air that gets sucked into your negative pressure grow room? My gut says yes. My brain asks how. I'm planning on using a grow tent for the time being. I hope you can answer! Thank you again, for doing what you do.
yes I like using the purple automotive hepa filters going into the resevoir and it’s important to exhaust the grow tent outside. With that setup you won’t have issues because the grow space will be completely separated by the tent and contained air
ill be grabbing some of those Blue Oysters from you for sure. I bought some Pink Oyster from you off Etsy a couple months ago, they are fruiting now and they are absolutely GORGEOUS! my wife will be making a dish out of them for Thanksgiving dinner, I can't wait! Fast growers and very stable. your QC is top f'n notch. thanks brother!
Im looking forward to seeing your lab when its done, you really know your stuff, and thank you for sharing your knowledge with us!
glad they worked out for you! Thanks for watching and following along! MUSHLOVE
thank you for such a beautiful briefing appreciate your efforts. much love to you too.
Electrical outlets are often overlooked as sources of dirty air sneaking into a space. Sealing them with Silicone is quite effective. Take the faceplate off, remove the outlet from the box and seal all air holes in the box and around it. Then reinstall the outlet and faceplate.
nice tip! thanks a ton 🍄❤️
Mate great video. Thanks for helping me out
Nice new set up
I have a question that maybe you can answer. Can mycelium overpower contamination or even eat the contamination? I've seen heated debates on this but I'd like to know your opinion as you're clearly educated in the field.
I have had jars of mycelium show signs of contamination in which I quarantined them and it looked like the mycelium won in the long run so I went ahead and fruited them successfully for 2-3 flushes without contamination appearing. I've read that once bacteria sporulates within the mycelium it's there to stay but I'm just wondering how safe it is to actually fruit mycelium that this has happened to.
I saw someone doing a study where they were trying to train mycelium to have greater resistance to contamination by allowing the contamination and mycelium go on agar until the mycelium took over and then transferring and then fruiting a generation and then running the same experiment again with hopes of building up a resistance. Is this futile or does it have merit?
If I was closer to where you are located I would 100% attend your classes that you do but unfortunately I'm not.
Thanks for taking the time to answer (if you do).
Mush love.
I will need to research more to have a clear answer on this - there are exudates that mitigate contamination (i.e. antibiotics) but Im not sure if the mycelium eats the bacteria or not - there are mushrooms that consume worms in soil (nematodes eaten by king stropharia) but they are larger in size than bacteria. I would not use contaminated mushrooms
of any kind because there is the potential for getting sick or even consuming mycotoxins from pathogenic molds etc. my philosophy is “when in doubt throw it out” hope that helps!
@@FreshfromtheFarmFungi Absolutely. Thank you very much for taking the time to answer.
I like your videos thank you so much
Gold
Good video buddy
The best choice is positive pressure
The device that creates suction from running watet is a venturi.
cool thanks! I had a brain lapse but at least someone knew what I was talking about hah 🍄❤️
@@FreshfromtheFarmFungi Did you ghost my reply where I disagreed with you about the BSL-3 lab being negative not positive.
pressure that you stated?
i am sorry you got confused on this point but you are wrong.
Positive pressure would cause a possible escape route for the potentially infectious agent at every leak or crack in the walls/enclosure. Instead negative pressure assures that all the air escaping the room goes through the HEPA filter and gets trapped before it can escape.
Please look it up.
@@ghz24 You are wrong, there is a tool called a manometer that we used every day to check pressure for our clean rooms in the last BSL-3 Lab that I worked at. Every room was positive pressure, but the overall "core" of the lab was filtered and had air locks to prevent any air from escaping. It is more specific than I stated in the video, yes, but all rooms were positive pressure to maintain the sterility and cleaned thoroughly after each procedure and tested regularly to maintain the status of BSL-3.
Was it on here that I heard about the water inside hyphae being at 4 atmospheres of pressure??
Not here but that is a cool
fact if it’s true! 🍄❤️
Dommage qu'il n'ait pas laissé le père répondre à la question "vous n'avez pas peur que votre fille vous reproche un jour ce choix?"
Comment un enfant de 8-9 ans peut-il savoir ce qu'il va faire dans 10-15 ans ?
Est-ce que ce choix ne limite pas les possibilités de choix pour ces enfants quand ils seront plus grands?
I like the shirt!!
thanks! They are available on our ETSY etsy.com/shop/freshfungi
@@FreshfromtheFarmFungi
Sweet Thanks!!