Easiest way to create POSITIVE PRESSURE in your Mycology Lab

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  • Опубликовано: 22 окт 2024

Комментарии • 89

  • @MrStevekilla
    @MrStevekilla 2 года назад +13

    I do this in a sealed off 3'x3' grow tent as a newbie mushroom grower, I haven't had any contamination. Thanks for showing us!

    • @ranjanty
      @ranjanty 2 года назад +2

      Thanks for commenting it encourages me. I'm just starting also, haven't done anything yet but ordering supplies and waiting for a 4'x4' grow tent to arrive, also lions mane LC in a syringe. I was thinking about trying to use the grow tent to do everything in because I'm very limited in space. I will have to set it up in my bedroom. It will be my lab, inoculation room and grow room. So I guess if it worked for you it should for me.
      It amazes me that there are so many different ways of doing this, from making agar to substrate mixes. seems like every RUclips channel uses a different method. I do have one ? Do you use a S.A.B. inside the grow tent?

    • @MrStevekilla
      @MrStevekilla 2 года назад

      @Randy L No I personally don't use a S.A.B. (I treated the grow tent before I had positive pressure as a still air box) I just wear a mask to stop my breath, and clean everything with 70% iso and never had any contamination. I'm in a similar spot I live in a apartment so I have very limited room it works very well for my situation! Happy growing!

    • @ranjanty
      @ranjanty 2 года назад

      @@MrStevekilla when you say you treated the tent. I was thinking about spraying the tent with silicon spray to make it water proof especially on all the seams. Is that what you mean by "treating"? I'm also going to use a IFD ( ionized)air filter I already have to clean the air.

    • @Shitstain951
      @Shitstain951 Год назад

      @@ranjanty human California prop 65 cancer warning on that ozone “ionized air” or o3. Will cause cancer if breathed

  • @probiogr3863
    @probiogr3863 2 года назад +5

    This guy always has the answer before i even ask!

  • @fatkorn
    @fatkorn Год назад +1

    You need to run that 24/7 or sterilize the room every time you start up but by looking at you not in a suit with your hair covered you only need to air curtain your work space or use a clean box. We use grow tents, you can throw out tents if needed an a abrasives cabinet for a glove box an a hydrogen peroxide fogger acquired from a hospital, nothing escapes hydrogen peroxide vapor. Keep growing broX, the much love is classy.

  • @jessepayne4832
    @jessepayne4832 2 года назад +2

    As an HVAC professional and a mushroom newb. I am going to experiment with the positive pressure needs of my lab by ducting a 6" pipe from my suction side of my flow hood to outside of the lab. It will be sealed and have a Merv 13 pre filter. This way the flow hood is mostly recirculating in the lab, but it will also be pulling in prefiltered fresh air creating positive pressure in the lab. Mossy creeks old setup had something similar. 2 birds with 1 stone(fan). Hopefully this doesn't negatively affect the longevity of my flow hood HEPA filter.
    Thoughts?

  • @josephpatton1757
    @josephpatton1757 2 года назад

    Yeah I’ve been using these for years, I also use them for my grow tents also. So that way clean filtered air is going into my tent, less contamination. Maybe overkill, but it works well.

  • @necrophagus9
    @necrophagus9 Год назад

    Vivosun products are dope. Im using a 10x10 grow tent as my mycology lab 😂 their fans aren't necessarily the best you can buy but for the price I've not had any problems with them. Definitely work as advertised.

  • @ThePinkBinks
    @ThePinkBinks 2 года назад +4

    Perfect timing! Exactly what I was going to research today. 😁
    Not just the Mushroom King but the best teacher on actual lab work. Awesome!
    Hey are those H14’s or H13’s? (I’m having trouble getting H14’s in my part of the world but H13 should be plenty for the room rather than my flow hood, right?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      you would want to add this in addition to a flow hood but it could work just be clean and do more than expected to make up for any contamination

  • @jodysephus6922
    @jodysephus6922 2 года назад +1

    Love your videos and have obtained much info from you over the past several months so thanks a heap.

  • @Sgtspork
    @Sgtspork 2 года назад +4

    I see a major flaw. I use almost exact same set up for my clean box.. for the exact same reason. Just a different HEPA filter and I use a prefilter.
    But it IS IMPORTANT that the HEPA filter is LAST in the chain.
    One thing, put the HEPA filter last, so the air is pushing through it. I also use a 4" inline vivosun fan and 4" clear PVC/Vinyl ducting.. on the intake, I have a steel carbon prefilter used for grow tents (vivosun, etc), they are about $35 bucks or so if I recall. You can use a port thru the door for the flex PVC duct..
    The reason you want the HEPA last is because contaminates can get into the works past the filter.. this actually happened to me with my clean box version 1.0 and took me forever to figure out.. contam had somehow got into some nook or cranny that was being missed by a thorough disinfection (full bleach and alcohol cleaning)!!! So I was ending up infecting my samples just by using the clean box! (Thinking things HAD to be sterile or near sterile, well when I poured agar plates and had a 70-80% contam rate.. I figured it out that it was the air system. I bought the prefilter to affix to the air intake side of the inline fan, switched to flexible PVC/vinyl ducting which was more durable and easier to clean and put the HEPA filter at the end of the air system chain, with the air flow pushing through it rather than sucking it in.. and my contam rate went down to less than 1% to zero even on my last pour.. and this is a large 120qt sterilite tote with two 5" open air arm holes (plastic toilet flanges for strength), and a slightly modified lid where I put window stripping (foam material that compresses) and three ratcheting flat tie down straps. To create a positive air pressure barrier with the sterile HEPA filter air system. It's basically a diy laminar flow box.. that uses $20 air purifier h13 HEPA filters which I can replace every six months.. not the most sterile you would think but this works like a charm..
    See, here's the reason this works best..
    First, having the prefilter removes all the larger particulates, such as dust, bugs, etc which would further clog the HEPA filter.. now with the prefilter, all the HEPA filter has to do is filter the really fine .2 micron stuff, germs, spores, etc.. and will last longer before cleaning or replacement.. the chrome round grill filters common for grow tents are really good as well and cheap and you can replace the internal filtering materials (carbon, etc) when the time comes..
    Second and most important, having the HEPA filter last in the chain means that if somehow a contaminate does get into your closed air system somehow past your prefilter or through damage to the ducting or other random reason... You are still protected as unless there is a failure with the HEPA filter itself, it's the last thing in the chain catching everything going thru the system.. it removes all of the real estate of the air system as a potential contamination point since it is now the terminus for the air.. if you had the filter first and a bad seal where the fan is joined, it defeats the point of the HEPA filter as you now have a secondary intake, if you have the same fault where the prefilter is bypassed, there is no harm as the HEPA filter will still be doing its job. Hell, the whole positive air system could get contaminated by spores and you'd still have the HEPA filter terminus doing its job protecting the lab from them. It is just a more robust logical design that I found out the hard way about in my home lab. HEPA filter first as intake isn't good, it should be the terminus as they are in flowhoods and the like. And the cost is minimal to make the adjustment. I used a variable speed inline fan to have more control to dial in what I needed in air flow better.
    Hope this helps, don't make the same design mistake I did.. plus the clear flexible ducting and prefilter will offer better mounting solutions as well..
    I can send you pictures and direct links to products if you like, just post an email where I can send it if interested. Cheers!

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      there would be issues placing the filter on the other end as well as over time there may be leaks that happen where the clamp is connected to the fan and then unfiltered air can be pushed into the lab - this method is not perfect by any means and like I said in the beginning there are better ways to make positive pressure that are the golden standards in all professional labs this is just a cheap and easy way to ad an extra layer of protection to the lab.

    • @Sgtspork
      @Sgtspork 2 года назад +1

      @@FreshfromtheFarmFungi sorry, I do tend to be wordy and overly dramatic even, lol, and my main point gets lost often..
      let me sum up more clearly.
      adding a prefilter on the intake and a change in the direction of the airflow so the Hepa is the terminus of the air vent system will help strengthen the contamination filtering properties of your positive air flow system as well as reduce overall filter maintenance as it is a very cheap and minimal upgrade for the effectiveness it gives.
      is basically only just what I meant to say, it just got caught up in a bunch of emotion and frustration from my long journey this year to find this out..
      please excuse my seeming dramatic hyperbole in my prior post, it was well intentioned but there was a lot of emotion and frustration built up behind it due to the issue I had described in my former post in having a HEPA as intake design to my similar system.
      it literally caused me a ton of money, time, and effort (and thus major frustration) as a contaminate hiding within the system itself was unable to be filtered as it was already past the HEPA on the intake.
      literally eight months of hell and many lost grows and grain spawn while I systematically tried to track down the issue by reviewing and refining all other aspects of my technique from grain spawn media production, inoculation techniques, pasteurization of substrate methods, etc..
      I literally had to tear my whole system down and rebuild it, only to find it was the one place I hadn't considered and if I had had a better design, it wouldn't have occurred at all. I am just now literally about to have my first successful harvest after eight months in the next few days.. after months and months of failure and going to considerable and sometimes ridiculous lengths to ensure contamination did not occur..
      it would happen at various stages, not just one, sometimes grain spawn would be fine and mature properly only to have contam set in when I spawned a bin.. other time large batches of spawn bags would go sour.. and I wasn't new at this.. not claiming to be an authority but I knew I was already operating within acceptable tolerances. I changed vendors for materials. any vector that this level of contam could be regularly getting into my grow cycles, I looked at except till I narrowed it down and had proof that it was my HEPA air system.
      it would be similar to finding a hidden fault in your laminar flood hood as it's just something you trust works if you follow proper technique and it looks in working order and you follow good maintenance procedure. you wouldn't think at first that it might be the issue.. it just goes to show that you can't take anything for granted.. and I offer up my story here as cautionary tale to those that read it and also to explain why I stressed it so vehemently in my initial post as I had a lot of frustration and such built up over it..
      of course, do as you see fit and you had a specific instructional purpose for your vid and it was a good one. it just intersected with my own long standing issue and the fact that I'm finally just on the literal cusp of putting a long nightmare behind me... almost holding my breath because I'm worried something will go wrong, lol. as it had so many times in the past month..
      I did intend just to give the hard won design advice but I did not mean to overemphasize or become hyperbolic while doing it and my apologies for that. my intentions were good, my execution not so much. it's been a bit of a rough year.
      one boon tho of having to track this issue down, despite all the frustration it's brought me was that I was able to review and refine many parts of my grow cycle techniques and really up my efficiency and make improvements.. so there's at least that.
      mush love.

    • @positivevibesveda
      @positivevibesveda 2 года назад +1

      @@Sgtspork i think your original message was shorter😅

    • @Sgtspork
      @Sgtspork 2 года назад

      @@positivevibesveda 🤣

    • @dpratte
      @dpratte 2 года назад

      @@Sgtspork A very good and helpful discussion. Thanks!

  • @fungifocus
    @fungifocus 10 месяцев назад

    thank you so much for all of your super informative videos!❤

  • @georgecampos7119
    @georgecampos7119 Год назад

    How big is your lab.
    Nice work brother keep the good work and mush love God bless you

  • @highlandmary33
    @highlandmary33 2 года назад +2

    Oh these fans are MUCH more economical method than an intensive & expensive pro HVAC system! Probably uses less energy resources too.
    Thank you!!

  • @BunnerzLoverz
    @BunnerzLoverz Год назад

    Love your videos Gary , im also in Denver , im making a positive pressure room now and following your instructions 😁

  • @mattnsim
    @mattnsim 2 года назад +1

    I’ve got this setup in my hydroponic tent lab. It’s only 8m2 and I use a 6” fan. It literally blows it up like a balloon

  • @Mariagonzalez-vp4lh
    @Mariagonzalez-vp4lh Год назад

    hello Gary, i cant find the hepa filter you recommend. could you tell me whats the brand? thank you!

  • @user-fe8gk2xb2t
    @user-fe8gk2xb2t 4 месяца назад

    thanks for posting this! recently got some of you Blue oyster commercial LC. trying to get a lab built up before it arrives. one quaetion: can that filter be used fobr exaust out of a tent to catch spore load. ill have a tent in a basement.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  4 месяца назад +1

      I wouldn’t recommend it - if possible vent outside it will save you tons of headaches with contamination issues and the health of your labspace/grow space

  • @javierross7441
    @javierross7441 10 месяцев назад

    Is that fan fitted to drag air through a closed off area like a water closet? If so it's going to force the fan to work too hard, increase the noise it makes sucking, and drastically shorten it's life.

  • @glennforister84
    @glennforister84 2 года назад +1

    Awesome idea

  • @jennymckinney7175
    @jennymckinney7175 2 года назад

    What a great simple solution

  • @trevorfichtner3539
    @trevorfichtner3539 Год назад

    thats a nice filter, they cost like $170 though, personally i chose to metal tape a hepa filter insert ($30) sealed to the wall of the tent, forcing all the air only through the filter before going in the inline vent fan, and sealed the sleeve around the vent, with about 8 or 10 inches of venting sealed to the vent inside the sleeve, to keep the negative pressure from pulling the sleeves inward, restricting airflow. saved $140 this way. although i am a newbie, first time with a tent, i see no reason this will be an issue, but i will post an update to this comment should a problem arise from this.
    btw, to FFTFF, i left a question in comments today in another video of yours (titled "deep cleaning the shroom room" reguarding bleach concentration, if you see this please answer that other one asap, i need a little clarification that i think could make a big difference, thank you!

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Год назад +1

      cool idea! Yes that would also work, I just like the replacement process with the tube clamp it makes it easy and when I got these they were like 45$ to replace, there are other brands now too for less

    • @trevorfichtner3539
      @trevorfichtner3539 Год назад +1

      @@FreshfromtheFarmFungi what brand are you using for that?

  • @cestlaviehappner1872
    @cestlaviehappner1872 Год назад

    I can't find those filters on Amazon have any way you can shoot me a link

  • @suneettalukdars7256
    @suneettalukdars7256 2 года назад +1

    Do we still need to use lanimnar flow for culture works, If there is positive airflow in the lab ?

  • @theruralgardener123
    @theruralgardener123 2 года назад

    Thanks for the info Gary! Is the air that you are pulling in to your lab to create positive pressure have to be air from outside or just a room outside the lab? My lab is inside an enclosed space inside a big metal barn. Thanks, Rob

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      The air is from the stairwell which comes from the upstairs which comes in through seams in the windows/doors and probably other areas too - It will eventually make its way in from outside so I think the barn would work it’s more of a temperature issue at that point (would outside air be too cold/warm for the lab) Hope that makes sense! 🍄❤️

  • @F3PIZZA
    @F3PIZZA Год назад

    Where do you have the Bludri integrated?

  • @F3PIZZA
    @F3PIZZA Год назад

    So an inoculation station needs to be positive pressure and a fruiting station needs negative pressure?

  • @spacetimearchitect3318
    @spacetimearchitect3318 2 года назад

    Great video as always!
    Did you take the listing down for your Lions Mane cultures on etsy? I want to make an order today.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +2

      they sold out over the weekend I posted another batch though this morning 👍 they are in high demand

    • @spacetimearchitect3318
      @spacetimearchitect3318 2 года назад

      @@FreshfromtheFarmFungi that's awesome man I'm glad you're doing well. 🍄❤

  • @funghi-farm
    @funghi-farm Год назад

    Oh wow yes perfect good idea!

  • @dhaval4570
    @dhaval4570 2 года назад

    nice solution easy to execute! by the way garry I recently collected a spore print from a bag that had bacterial contam, cause the fruits were miniature but I managed to get a spore print from it. Is this spore print safe to use or will the previous history have some effects on these spores? like will the fruits from this print be small too ?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      there should be enough variation from the print to produce some larger some small and some medium sized fruits - then you should clone the ones you like best for consistency after 👍🙂 hope that makes sense!

    • @dhaval4570
      @dhaval4570 2 года назад

      @@FreshfromtheFarmFungi yupp! Variation as its a multispore growth. I was mainly worried about the bacterial fruit the print came from, thanks for clearing the doubt!

  • @joellerondeau4009
    @joellerondeau4009 Год назад

    Do you have the Amazone link for the filter ?

  • @TayyabMohammadtay
    @TayyabMohammadtay 2 года назад

    hi the clip your were willing use to attache hepa filter with 4 inch fan is called the jubilee clip. please pardon me if it hurts your feelings , knowledge belongs the one who seeks it. much love to you too.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад

      thanks for the info! feelings were not hurt - I know very little in the grand scheme of things 👍

  • @eyesdontsee
    @eyesdontsee 2 года назад

    Mush love from Lakewood!

  • @gabriellelapalme1417
    @gabriellelapalme1417 2 года назад

    Does the positive pressure need to run 24/7 or just when you walk in or use the lab space?

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      24/7 is best to prevent insects from getting in so it depends on your lab location and environment

  • @TecraTube
    @TecraTube 2 года назад

    forgive my ignorance in fluid dynamics, but isn't air coming into the room considered negative air pressure and not positive? I thought positive air pressure was when the inner air of the space was being evacuated outwards.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад

      opposite, because the air being forced in creates a pressure against the walls of the room so air gets pushed out through cracks and crevices like under the door or window frame. You want to eliminate contaminants from entering the system

  • @warlock1005
    @warlock1005 Год назад

    What are your thoughts on a fresh air exchange?

  • @ranjanty
    @ranjanty 2 года назад

    what are the measurements of your room? I ask to know if the 4in. inline fan would work in my room. thanks

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      it’s a small lab about 10’x10’ with a nook for the other flow hood

    • @ranjanty
      @ranjanty 2 года назад

      @@FreshfromtheFarmFungi Gary thanks for replying and sharing your knowledge with the Mycologist wannabe's like me. I'm strictly doing this for myself to make LM tincture, I'm 68 and find this to be fascinating like Beekeeping in my younger days and it's not as physical.

    • @josealvarez8566
      @josealvarez8566 2 года назад

      @@FreshfromtheFarmFungi
      Hi Gary, 10x10 inches or 10x10 feet? thank you for your video.

  • @lirg123
    @lirg123 2 года назад

    Cool air filters, super charger, heat power turbo

  • @RichardBronosky
    @RichardBronosky 5 месяцев назад

    1:11 you call it a "utility door". Is it a utility room (aka closet)? If it's a sealed closet, it's not causing positive pressure, because the air that fan removes from the closet it getting replaced by air in the room. You should reply to clarify and pin this comment to save others from making a mistake [that I presume you wisely avoided].

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  5 месяцев назад +1

      it is not a closet - it opens up to the other side of the basement but it is a separated space because the water tank is blocking access from the other side, I should have filmed the inside of the closet to show but yes you are right that if it was sealed it wouldn’t be positive pressure but just a filter

  • @ericbarrus4455
    @ericbarrus4455 9 месяцев назад

    Cmf per cubic feet?

  • @josefhuber5699
    @josefhuber5699 2 года назад

    Could I take a activated carbon filter?

  • @josephpatton1757
    @josephpatton1757 2 года назад

    This what I’ve been doing for awhile now actually

  • @klaymistic4810
    @klaymistic4810 Год назад

    Doesn’t putting a fan in your lab, create turbulent airflow, which causes contamination

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Год назад

      it can effect airflow but if it’s filtered it shouldn’t effect contams, ideally these would be in the ceiling though away from the workspace

  • @MyHomeUSA
    @MyHomeUSA 3 месяца назад

    I'm sorry to be the bearer of bad news but, if it's a closed closet to which you applied the positive pressure HEPA filter/blower, then It's a closed loop, and you've added neither positive nor negative pressure to affect in your lab! The closet will draw air from your lab into the vacuum environment within, through every conceivable draft opening around the door. In fact, you've added an Ineffective air purifier to the lab, and that's because it will steer up resting closet particles which will become airborne and which the HEPA filter will need to suck up...

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  3 месяца назад +1

      @@MyHomeUSA it’s not a closet I should have shown what’s behind the door lol it leads into the larger area of the basement which has access to the upstairs so the air comes from above and down through the stairwell, through the “closet” which is a utility area through the filter and into the room. Hope that makes sense!

  • @maple2962
    @maple2962 Год назад

    not incredibly idle as youll be bending your airflow.

  • @tylerallen9856
    @tylerallen9856 2 года назад

    Lol, definitely consult with the wife

  • @jacobfriedrich835
    @jacobfriedrich835 6 месяцев назад

    Consult your wife 😂

  • @LongDefiant
    @LongDefiant 2 года назад

    Where's the FLOW hood, bro? Still down? 😿

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +1

      it is right behind me 👍 both are operational this is just an additional layer of protection to the lab

  • @quadlawnmowerman
    @quadlawnmowerman 2 года назад +3

    Lol, clearly not a hepa filter, someone just whacked a "hepa" name on the filter, have a closed look champ.
    Should of been obvious to you when they also put the word "organic" on the box as branding as well.......

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 года назад +2

      thanks for the algorithm boost! Unfortunately I don’t have a particle counter to challenge this statement and have to trust the manufacturer- there are other more traditional looking HEPA filters that fit on these fans as well - it’s more the concept that will help others achieve a cleaner lab and it only took 10 minutes to set up - there are obviously better ways to go about this, that is not the point of the video 🙂🍄❤️

    • @EV0L13
      @EV0L13 2 года назад +1

      The guy with all the brains is here!!🧠 He’s smarter than you and has 30 yrs of experience to back it up.😂
      Always one of these azzzhats posting nonsense if it’s a quality video.
      Lawnmowerman has zero created content online unfortunately. I’d love to checkout the skillz 😝
      You ever had a DUI on your quad-lawntravelerrr??

    • @jermlittle1970
      @jermlittle1970 Год назад

      Earnadd he can’t afford a riding mower but he’s so stupid he could get a dui pushing his lawn mower! I agree I can’t stand these morons and their 2 cent opinions.

  • @MutumDevarishi
    @MutumDevarishi 2 года назад

    This guy had earlier work for alien?🤔

  • @louisel-khouri1015
    @louisel-khouri1015 5 месяцев назад

    HAVE U EVER GREW PLANTS IN A GROW TENT?
    IF U USE POSITIVE PRESSURE UR BRINGING IN AIR FROM A LUNG ROOM THAT ITS IN & THATS NOT GOOD. U CANT CONTROL TEMP & HUMIDITY THAT WAY, U NEED NEGETIVE PRESSURE TO DO THAT & ALLSO KEEP MOLD ECT.
    WHY DO U THINK ALL HOSPITALS & SCHOOLS & SHOPPING CENTRES HAVE NEGETIVE PRESSURE & THE OFFICE I WORK IN!
    FILTERS ARE USED TO KEEP ALL IN CHECK FROM TEMPS & HUMIDITY & DIALED IN TO UR ENVIRO. POSITIVE PRESSURE, U WILL MEVER FIND UR SWEET SPOT ECT

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  5 месяцев назад

      these are good points - I think as a whole building yes, but in a micro condition and in most clean rooms positive pressure is ideal so contaminants don’t get into the system. I have grown many plants in tents and negative pressure is better suited so you can exhaust heat where in mushroom farming this might make sense in the fruiting rooms, if you had negative pressure in the lab you would be concentrating contaminants and spores into your lab. Hope that makes sense!

  • @ryanguy7399
    @ryanguy7399 4 месяца назад

    Lol hack