How a mini CRISPR Cas9 system is revolutionizing gene therapy!

CRISPR saCas9: It's the greatness of spCas9 "miniaturized"!
➜ Dear fellow gene editing enthusiasts, thanks for following our Molecular Minutes series--we hope you've had many gene editing adventures since! Have you heard of saCas9? It's a newly discovered nuclease that could change the game for CRISPR-based gene therapy. This "little" nuclease is small enough to fit into an adeno-associated virus and has already been busy editing the genomes of living organisms. Exciting, right? Watch our video to learn more about this CRISPR Cas9 system!
Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse: info.abmgood.co...
abm now carries saCas9:
➜ Proteins: www.abmgood.co...
➜ Vectors/viruses: www.abmgood.co...
For more info on the CRISPR Cas9 system, including the spCas9 and saCas9 nucleases, please visit:
➜ Our Knowledge Base: info.abmgood.c...
➜ All CRISPR Resources: info.abmgood.c...
Check out our other video series:
➜ Polymerase Chain Reaction (PCR) - An Introduction: • 1) PCR (Polymerase Cha...
➜ CRISPR Cas9: • CRISPR Cas9 - A Brief ...
➜ Adeno Associated Virus: • 1) Adeno Associated Vi...
➜ Cell Culture: • Cell Culture
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➜ Google+: goo.gl/5bRNwC

[@newzealandgold4734]

Love the video, harder than it looked to make.

[@the_encephalonaire]

Please make a video on magnetic bead DNA extraction methods and steps.

[@abmgood]

That sounds really an interesting topic to cover. We will definitely let the video production team know! Thanks for your suggestion.

[@the_encephalonaire]

@@abmgood ok thanks.

[@edpaek]

Good job on the video. This was a fun update. Could you leave links for PubMed references to the papers you reference?

[@abmgood]

Hi Daewon774,
We're glad you like the video we made! And sure, here are the links to the reference papers:
Chen, Baohui et al. "Expanding The CRISPR Imaging Toolset With Staphylococcus Aureus Cas9 For Simultaneous Imaging Of Multiple Genomic Loci". Nucleic Acids Res 44.8 (2016): e75-e75. goo.gl/sjj5hj
Kaminski, R et al. "Excision Of HIV-1 DNA By Gene Editing: A Proof-Of-Concept In Vivo Study". Gene Therapy 23.8 & 9 (2016): 690-695. goo.gl/oduIID
Ran, F. Ann et al. "In Vivo Genome Editing Using Staphylococcus Aureus Cas9". Nature 520.7546 (2015): 186-191. goo.gl/vM8mpu
As well as our AAV Knowledge Base: goo.gl/F7lvrL

[@johnluzano4724]

subscribed, cool content.

[@abmgood]

Thanks :)

[@samuelzev4076]

Question, can Criapr be applied as a treatment for conditions like myopia?

[@abmgood]

Hello Samuel,
Unfortunately, myopia can not be corrected in an adult eye by genetic editing. Modifying the instructions/DNA can stop the problem in future, but it is not possible to fix things which are already defected.

[@samuelzev4076]

Applied Biological Materials - will there ever be a cure for myopia using gene editing?

[@rahulpaul2976]

Is it possible to change skin color by using crispr cas9 gene editing system??

[@abmgood]

Hello Raul,
Yes, theoretically the CRISPR gene editing system could be used to modify melanin expression levels.

[@nebiu9662]

is crispr cas 9a device?

[@abmgood]

Hi Nebiu, thanks for your comments! Cas9 is a nuclease protein that is currently popularly used in CRISPR gene editing. You can read more about Cas9 variants at our knowledge base: goo.gl/df55ap

[@KingJimmyC]

Who knows how to do use a crispr cas 9 device?

[@abmgood]

Hi James! Thanks for watching and commenting -- there are many scientists in the field as well as companies that now offer CRISPR Cas9-related services. At abm, for instance, we offer sgRNA design services as well as a variety of cells that express Cas9. You can find our CRISPR products/services here: www.abmgood.com/CRISPR-Cas9-sgRNA.html