Can b-cells be extracted from a human spleen? The monoclonal antibodies they are capable of binding and neutralizing antigens are they also able to become immune cell receptors ? The rate of replication and deterioration of the lungs of CV19 results in antibody deficiency in part to the delivery of immune cells and cytokines to the lungs a result of accumulative debris and waste constricting capillaries and arterioles leading to the lungs. Antibodies are not mobile, immune cells are mobile. Constriction of the capillaries and arterioles leading to the lungs inhibits the delivery of larger immune cells restricting travel through the capillaries due to their size disregulating the innate response and increasing leukocyte count in the bloodstream .
But why do I feel bad when you say the mouse is injected several times and is sacrificed😥😥. Of course I know it is very necessary but the mouse you are showing is so cute
Hi! Your video explains really good the hybridoma technology and makes it easy to understand! I am a student of medical laboratories and i am writing a project about the monoclonal antibodies and i was wondering if you could send me the pictures you have in the video so i post them in the project as i want to explain the hybridoma thechnology too. It would be very nice if you were able to reply ASAP. Thank you
Thank you for your valuable feedback. Regarding your query, I am afraid that right now I'm not distributing the content of these videos in any kind of format. Thanks.
a really like your lecture, but i have a doubt: what would it be hose fused b cells and fuses myeloma cells? just cells from the same type that had suffered fusion? thanks for your help
No, it's not to increase specificity of antibodies. Antibody producing B cells (Plasma cells) have a short life span in cell culture medium. Our requirement is to produce large quantities of monoclonal antibodies. So, by fusing these B cells with myeloma cell we make these Plasma cells immortal.
There are two reasons for this... 1) cells for fusion are exposed to PEG for a very short duration 2) And only few cells actually fuse in this process Moreover, "fusions between two or more cells are unstable." (reference Owen Kuby Immunology)
Your lectures are very good but it would b really nice if u use human voice :)
+AYUSH thank you for your feedback.
Can b-cells be extracted from a human spleen? The monoclonal antibodies they are capable of binding and neutralizing antigens are they also able to become immune cell receptors ? The rate of replication and deterioration of the lungs of CV19 results in antibody deficiency in part to the delivery of immune cells and cytokines to the lungs a result of accumulative debris and waste constricting capillaries and arterioles leading to the lungs. Antibodies are not mobile, immune cells are mobile. Constriction of the capillaries and arterioles leading to the lungs inhibits the delivery of larger immune cells restricting travel through the capillaries due to their size disregulating the innate response and increasing leukocyte count in the bloodstream .
Just keep going man ...one day your hard work will pay off 🙌u make perfect explanation step by step🙏💕
Thanks
Monotonal Antibody discussing Monoclonal Antibodies!!
Mam please explain. Chimeric monoclonal antibody
Thanks. I got some knowledge from this .
Beautiful presentation. Pls do more videos on life science subjects
Thank you for your feedback.
Thank you so much! That was a great explanation!
+Nour Khattab Thank you for your feedback.
good work
Baby shalini
It's very nice exaplanition mam
But use your voice to explain mam
I am understand clearly mam
I have an exam tomorrow. This was immensely helpful. Thank you.
aren't the "myeloma cells" from *_human embryo_* lines like HEK293?
Yes a human voice would be absolutely splendid.
its excellent but pls change voice make it realistic
excellent description of topic
I felt stupid for not developing this tech lol
This is the best video, well explained. God bless you sir
Your videos are the best!!!! Keep up the good work...! Really helpful man! Kudos
Loving ur video starting tune😍😍😍
The mouse is SACRIFICED 2:25
plz make a vedio on class switching(immunoglobulins)
Elaborative, Specific and cristal clear conception. Thanks
Awesome vdo ..well understood ..thks a lot ❣️💫
This was so easy😭😭❤️❤️❤️ thanks a tonne
Thank you for your valuable feedback.
Supub.... Thanks.. It's very helpful for deep understanding... Carry on the good work... Hats off to you
Thank you for your feedback.
Easily comprehensible!
Thank you very much!
Outstanding! Love it!!
excellent explanation!
But why do I feel bad when you say the mouse is injected several times and is sacrificed😥😥. Of course I know it is very necessary but the mouse you are showing is so cute
u literally saved my semester keep up the xllent work :D
May Allah bless you
Hi! Your video explains really good the hybridoma technology and makes it easy to understand! I am a student of medical laboratories and i am writing a project about the monoclonal antibodies and i was wondering if you could send me the pictures you have in the video so i post them in the project as i want to explain the hybridoma thechnology too. It would be very nice if you were able to reply ASAP. Thank you
Thank you for your valuable feedback. Regarding your query, I am afraid that right now I'm not distributing the content of these videos in any kind of format. Thanks.
@@FrankLectures It's ok! The video helped me a lot so thank you anyway.
Thanks
Nicely explained
Perfect😛😜😜
Excellent. Keep it up. Please make more videos on biotechnology. This is my first video watching of your channel..n subscribed the channel.
thank you so so much it was very well explained ^-^ !!!
a really like your lecture, but i have a doubt: what would it be hose fused b cells and fuses myeloma cells? just cells from the same type that had suffered fusion? thanks for your help
I just love all ur explaination its so precise and yet so detailed and gud... Thank you
Thank you for your feedback.
are the monoclonal antibodies of same type?like only IgG or Ig M or others.
the way of demonstrating via figures is so great whereas the sound is not good, use human voice !
Absolutely smashing lectures!
Thank you for your valuable feedback.
Very nice analysis
Great lecture
Glad to know this. Thank you for your feedback.
Amazing I understant it🥺🤩thanqu
Very good knowledge...
Thanks
Nice lecture but what is the time frame between each step?
Very veryyyy niceeeeeeee.... Reallly helped a lot 👌🏻
please upload some video on antibiotics
Videos on antibiotics will be uploaded later when I'll begin lecture series on Microbiology.
Thank you
Ok . Thank you ...Sir
Thank you🙏
Really helpful 👍👍
Thanks for your video...
Thanks for a Very good video
Can't thank you enough.
You're Welcome
Superb explanation
Thank you for your feedback.
Why spleen cell?
Very easily explained
Tell me one thing... the reason of adding myloma cell is to only increase the specificity of antibodies isn't it
No, it's not to increase specificity of antibodies.
Antibody producing B cells (Plasma cells) have a short life span in cell culture medium. Our requirement is to produce large quantities of monoclonal antibodies. So, by fusing these B cells with myeloma cell we make these Plasma cells immortal.
@@FrankLectures It's FUCKING METAL AS FUCK DUDE, WE HUMANS ARE BRUTAL
Thank you so much
thanks a lot
thanks a lot
Good video 👍
Ultimate
please can you tell me why doesn't cell fusion by PEG occur between more then two cells
There are two reasons for this...
1) cells for fusion are exposed to PEG for a very short duration
2) And only few cells actually fuse in this process
Moreover, "fusions between two or more cells are unstable." (reference Owen Kuby Immunology)
Tq
You're Welcome.
vry vry.....helpful....thnx a ton
Thanks
Thanks alot, it is excellent
+Ibrahimnabil Nabil thank you for your feedback.
Very well explained. Keep up the good work!
Thanks for such a good video ...this one is way better than shomu's biology video of same topic