Can b-cells be extracted from a human spleen? The monoclonal antibodies they are capable of binding and neutralizing antigens are they also able to become immune cell receptors ? The rate of replication and deterioration of the lungs of CV19 results in antibody deficiency in part to the delivery of immune cells and cytokines to the lungs a result of accumulative debris and waste constricting capillaries and arterioles leading to the lungs. Antibodies are not mobile, immune cells are mobile. Constriction of the capillaries and arterioles leading to the lungs inhibits the delivery of larger immune cells restricting travel through the capillaries due to their size disregulating the innate response and increasing leukocyte count in the bloodstream .
a really like your lecture, but i have a doubt: what would it be hose fused b cells and fuses myeloma cells? just cells from the same type that had suffered fusion? thanks for your help
Hi! Your video explains really good the hybridoma technology and makes it easy to understand! I am a student of medical laboratories and i am writing a project about the monoclonal antibodies and i was wondering if you could send me the pictures you have in the video so i post them in the project as i want to explain the hybridoma thechnology too. It would be very nice if you were able to reply ASAP. Thank you
Thank you for your valuable feedback. Regarding your query, I am afraid that right now I'm not distributing the content of these videos in any kind of format. Thanks.
No, it's not to increase specificity of antibodies. Antibody producing B cells (Plasma cells) have a short life span in cell culture medium. Our requirement is to produce large quantities of monoclonal antibodies. So, by fusing these B cells with myeloma cell we make these Plasma cells immortal.
There are two reasons for this... 1) cells for fusion are exposed to PEG for a very short duration 2) And only few cells actually fuse in this process Moreover, "fusions between two or more cells are unstable." (reference Owen Kuby Immunology)
But why do I feel bad when you say the mouse is injected several times and is sacrificed😥😥. Of course I know it is very necessary but the mouse you are showing is so cute
Your lectures are very good but it would b really nice if u use human voice :)
+AYUSH thank you for your feedback.
Yes a human voice would be absolutely splendid.
This is the best video, well explained. God bless you sir
I have an exam tomorrow. This was immensely helpful. Thank you.
Just keep going man ...one day your hard work will pay off 🙌u make perfect explanation step by step🙏💕
Thanks
Thanks. I got some knowledge from this .
u literally saved my semester keep up the xllent work :D
excellent description of topic
Your videos are the best!!!! Keep up the good work...! Really helpful man! Kudos
Elaborative, Specific and cristal clear conception. Thanks
Loving ur video starting tune😍😍😍
Outstanding! Love it!!
I just love all ur explaination its so precise and yet so detailed and gud... Thank you
Thank you for your feedback.
Absolutely smashing lectures!
Thank you for your valuable feedback.
Thank you so much! That was a great explanation!
+Nour Khattab Thank you for your feedback.
Easily comprehensible!
Thank you very much!
Awesome vdo ..well understood ..thks a lot ❣️💫
good work
Beautiful presentation. Pls do more videos on life science subjects
Thank you for your feedback.
Supub.... Thanks.. It's very helpful for deep understanding... Carry on the good work... Hats off to you
Thank you for your feedback.
Very veryyyy niceeeeeeee.... Reallly helped a lot 👌🏻
thank you so so much it was very well explained ^-^ !!!
excellent explanation!
Very nice analysis
Great lecture
Glad to know this. Thank you for your feedback.
Thanks for a Very good video
Excellent. Keep it up. Please make more videos on biotechnology. This is my first video watching of your channel..n subscribed the channel.
Nicely explained
Amazing I understant it🥺🤩thanqu
Thank you🙏
Really helpful 👍👍
Mam please explain. Chimeric monoclonal antibody
aren't the "myeloma cells" from *_human embryo_* lines like HEK293?
Very good knowledge...
Thanks
Nice one
Thanks for your video...
This was so easy😭😭❤️❤️❤️ thanks a tonne
Thank you for your valuable feedback.
Thanks for such a good video ...this one is way better than shomu's biology video of same topic
Very easily explained
Can b-cells be extracted from a human spleen? The monoclonal antibodies they are capable of binding and neutralizing antigens are they also able to become immune cell receptors ? The rate of replication and deterioration of the lungs of CV19 results in antibody deficiency in part to the delivery of immune cells and cytokines to the lungs a result of accumulative debris and waste constricting capillaries and arterioles leading to the lungs. Antibodies are not mobile, immune cells are mobile. Constriction of the capillaries and arterioles leading to the lungs inhibits the delivery of larger immune cells restricting travel through the capillaries due to their size disregulating the innate response and increasing leukocyte count in the bloodstream .
Very well explained. Keep up the good work!
Monotonal Antibody discussing Monoclonal Antibodies!!
May Allah bless you
Superb explanation
Thank you for your feedback.
Why spleen cell?
plz make a vedio on class switching(immunoglobulins)
Good video 👍
Can't thank you enough.
You're Welcome
Perfect😛😜😜
vry vry.....helpful....thnx a ton
Thanks
Thanks alot, it is excellent
+Ibrahimnabil Nabil thank you for your feedback.
The mouse is SACRIFICED 2:25
a really like your lecture, but i have a doubt: what would it be hose fused b cells and fuses myeloma cells? just cells from the same type that had suffered fusion? thanks for your help
Hi! Your video explains really good the hybridoma technology and makes it easy to understand! I am a student of medical laboratories and i am writing a project about the monoclonal antibodies and i was wondering if you could send me the pictures you have in the video so i post them in the project as i want to explain the hybridoma thechnology too. It would be very nice if you were able to reply ASAP. Thank you
Thank you for your valuable feedback. Regarding your query, I am afraid that right now I'm not distributing the content of these videos in any kind of format. Thanks.
@@FrankLectures It's ok! The video helped me a lot so thank you anyway.
Thanks
please upload some video on antibiotics
Videos on antibiotics will be uploaded later when I'll begin lecture series on Microbiology.
Thank you
Ok . Thank you ...Sir
are the monoclonal antibodies of same type?like only IgG or Ig M or others.
Nice lecture but what is the time frame between each step?
Ultimate
Thank you so much
thank u very much
Tell me one thing... the reason of adding myloma cell is to only increase the specificity of antibodies isn't it
No, it's not to increase specificity of antibodies.
Antibody producing B cells (Plasma cells) have a short life span in cell culture medium. Our requirement is to produce large quantities of monoclonal antibodies. So, by fusing these B cells with myeloma cell we make these Plasma cells immortal.
@@FrankLectures It's FUCKING METAL AS FUCK DUDE, WE HUMANS ARE BRUTAL
the way of demonstrating via figures is so great whereas the sound is not good, use human voice !
thanks a lot
Baby shalini
It's very nice exaplanition mam
But use your voice to explain mam
I am understand clearly mam
please can you tell me why doesn't cell fusion by PEG occur between more then two cells
There are two reasons for this...
1) cells for fusion are exposed to PEG for a very short duration
2) And only few cells actually fuse in this process
Moreover, "fusions between two or more cells are unstable." (reference Owen Kuby Immunology)
its excellent but pls change voice make it realistic
But why do I feel bad when you say the mouse is injected several times and is sacrificed😥😥. Of course I know it is very necessary but the mouse you are showing is so cute
I felt stupid for not developing this tech lol
Tq
You're Welcome.
thanks a lot