Using OptiSTEM+/AutoSTEM in Velox and Sherpa

Welcome to our latest tutorial on achieving atomic resolution STEM imaging using OptiSTEM+ and AutoSTEM within Velox and Sherpa UIs. This video helps to learn how to acquire high-resolution, atomically resolved STEM images with powerful capabilities of OptiSTEM+ on the Spectra 200, Spectra 300, Spectra Ultra, Spectra Iliad and other Thermo Scientific microscopes.
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We start by demonstrating how to orient your sample in STEM mode using the SmartCam and click-tilt functionality within Velox. Next, we call OptiSTEM from Velox to automatically stigmate and focus the image, resulting in high-quality atomic resolution STEM images. To enhance the images further, we utilize the DCFI feature in Velox.
In the second part of the video, we explore the more advanced capabilities of OptiSTEM within the Sherpa UI. Here, users can also correct coma and three-fold astigmatism, offering even greater precision in the imaging process.
This tutorial is recorded on the Spectra Ultra microscope with GdGaO samples. OptiSTEM/OptiSTEM+ are available for Spectra and Themis TEMs, while Talos customers can use AutoSTEM. AutoSTEM similarly corrects focus and astigmatism from Velox but does not support coma and three-fold astigmatism correction, as these require a probe corrector.
OptiSTEM/AutoSTEM is an automated and robust alignment software designed for the correction of first-order aberrations (defocus and 2-fold astigmatism). With the STEM imaging package, you can achieve atomic resolution Z contrast imaging more quickly and reliably, minimizing the uncertainty of manual alignment. This ensures lower electron doses on specimens, making it easier to image beam-sensitive samples. OptiSTEM includes the functionality to abort tuning and return to the original optical settings. OptiSTEM+ provides automated, reliable, fast and robust correction of first and second order aberrations (defocus, 2-fold astigmatism, coma and 3-fold astigmatism). This makes atomic resolution Z contrast, BF, ADF and iDPC imaging at the ultimate resolution of the system, more accessible to less experienced users and empowers more experienced users with faster time to result. OptiSTEM+ is optimized for use with a S-CORR probe corrector.
Join us as we explore these advanced imaging techniques and improve your STEM imaging capabilities!
Special thanks to Minda Chen from the Thermo Scientific Americas NanoPort for making this video.
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[@dvivek07]

The automation has reached to the point where even high school students can operate the microscope and obtain nice atomic resolution images.